Difference between revisions of "Team:Jiangnan"

 
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<li><a href="#">protocols</a></li>
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<div class="Jnavtitle">
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<li><a href="#">interlab</a></li>
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<a href="https://2018.igem.org/Team:Jiangnan/Safety">Safety</a>
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<li><a href="#">lab journal</a></li>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Hardware">Hardware</a>
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<a href="https://2018.igem.org/Team:Jiangnan/Team">Team</a>
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<img id="6img" src="https://static.igem.org/mediawiki/2018/e/e4/T--Jiangnan--normnavbar--team.png">
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<div class="Jnavdrag">
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<ul id="teamdropdown" class="dropdown-content">
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<li><a href="https://2018.igem.org/Team:Jiangnan/Team">Team Members</a></li>
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<li><a href="#">about us</a></li>
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<li class="divider"></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Attributions">Attribution</a></li>
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<li><a href="#">attribution</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Collaborations">Collaboration</a></li>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Human Practice</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Human_Practices">Overview</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Silver">Silver</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Gold</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Public_Engagement">Public Engagement</a></li>
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<li class="divider"></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Entrepreneurship</a></li>
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</ul>
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<div class="Jnavtitle">
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<a href="https://2018.igem.org/Team:Jiangnan/Model">Model</a>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan/Notebook">Notebook</a>
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<div class="Jnavdrag">
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<ul>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Notebook">Lab Book</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Protocol">Protocol</a></li>
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<div class="Jnavtitle" onmouseover="Jnavshow(this)" onmouseleave="Jnavhide(this)">
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<a href="https://2018.igem.org/Team:Jiangnan">Project</a>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Background">Background</a></li>
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<li><a href="https://2018.igem.org/Team:Jiangnan/Design">Design</a></li>
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<div style="position: relative;">
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<img src="https://static.igem.org/mediawiki/2018/f/f3/T--Jiangnan--main--logo.png" width="100">
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<img src="https://static.igem.org/mediawiki/2018/0/0a/T--Jiangnan--main_top.png" width="100%">
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<h5>Project Description</h5>
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<div style="position: absolute;top: 4em;width: 100%;">
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<br>
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<div style="text-align: center;">
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<p style="text-align:center">The goal of our project is to enable 3 features of our chassis cell, i.e.</p>
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<img src="https://static.igem.org/mediawiki/2018/a/a1/T--Jiangnan--main_logo.png" width="10%">
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<p style="text-align:center">high titration, suspension cultivation, and broad spectrum to reduce the production cost and increase the yield of viruses for vaccine production.</p>
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<h4 style="color: white;">Project Description</h4>
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<p style="text-align:center">The chassis cell we used here is. MDBK cells.</p>
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<div style="margin-bottom: 0; width: 60%; display: inline; position: relative;">
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</div>
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<p style="text-align: center; color: white;">The limiting factors of most vaccine companies, according to our previous investigation and survey, are
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<div class="row" style="width: 100%; position: absolute; top: 1200px; margin-bottom: 0px;">
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mulitifaceted costs in actual production process and long product lift cycle and other issues due
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<div class="col s4 center" style="display: inline;">
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to complex application process of new vaccines(cell line).<br/>
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<img id="sus" src="https://static.igem.org/mediawiki/2018/c/cf/T--Jiangnan--main--sus.png" width="150" style="position: relative;">
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The goal of our project is to enable three features of our chassis cell, i.e.,high titer, suspension cultivation, and
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<p></p>
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broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here is
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<img src="https://static.igem.org/mediawiki/2018/d/dd/T--Jiangnan--main--sustitle.png" width="150" style="position: relative;bottom: 20px;">
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MDBK cell.</p>
 
</div>
 
</div>
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<div style="width: 33.3%;float: left;">
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<img id="broad" src="https://static.igem.org/mediawiki/2018/b/b1/T--Jiangnan--main--broad.png" width="150" style="position: relative;">
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<a href="#broad"><img class="animated infinite pulse" src="https://static.igem.org/mediawiki/2018/0/06/T--JiangNan--broad.png" width="50%"></a>
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<p></p>
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<div><h4 style="color: white">Broad Spectrum</h4></div>
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<img src="https://static.igem.org/mediawiki/2018/a/ab/T--Jiangnan--main--broadtitle.png" width="150" style="position: relative;bottom: 20px;">
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</div>
 
</div>
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<div class="col s4 center" style="display: inline;">
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<div style="width: 33.3%;float: left;">
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<img id="high" src="https://static.igem.org/mediawiki/2018/9/96/T--Jiangnan--main--high.png" width="150" style="position: relative;">
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<a href="#sus"><img class="animated infinite swing" src="https://static.igem.org/mediawiki/2018/c/cf/T--Jiangnan--main--sus.png" width="50%"></a>
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<p></p>
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<div><h4 style="color: white">Suspension Culture</h4></div>
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<img src="https://static.igem.org/mediawiki/2018/d/d8/T--Jiangnan--main--hightitle.png" width="150" style="position: relative;bottom: 20px;">
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</div>
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<div style="width: 33.3%;float: left;">
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<a href="#high"><img class="animated infinite rubberBand" src="https://static.igem.org/mediawiki/2018/9/96/T--Jiangnan--main--high.png" width="50%"></a>
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<div><h4 style="color: white">High Titer</h4></div>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
 
</div>
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<div id="high_titer" class="scrollspy">
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</div>
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<div style="color:white;width: 100%;height: 700px"></div>
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<div id="broad" class="row">
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<img src="https://static.igem.org/mediawiki/2018/6/69/T--Jiangnan--main--high_titerforw.png" width="100%" height="700" style="position: absolute;top: 1400px;">
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<div class="col s7 offset-s1">
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<div style="position: absolute;top: 1600px; left: 40px">
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<h5 style="color: #2e7d32">Broad Spectrum</h5>
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<img src="https://static.igem.org/mediawiki/2018/d/d8/T--Jiangnan--main--hightitle.png" width="300">
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<p>Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.</p>
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<br>
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</div>
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<br>
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<div class="col s3">
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<p style="width: 700px;">Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titration of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titration. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titration was further increased in response to plasma irradiation. </p>
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<img src="https://static.igem.org/mediawiki/2018/4/42/T--Jiangnan--main--broadmove.png" width="100%">
 
</div>
 
</div>
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<img id="highmove" src="https://static.igem.org/mediawiki/2018/9/96/T--Jiangnan--main--high.png" width="400" height="400" style="position: absolute;top: 1550px;right: 40px;">
 
 
</div>
 
</div>
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<div id="suspension" class="scrollspy">
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<div id="sus" class="row">
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<div style="color:white;width: 100%;height: 700px"></div>
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<div class="col s3 offset-s1">
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<img src="https://static.igem.org/mediawiki/2018/e/e9/T--Jiangnan--main--suspensionforw.png" width="100%" height="700" style="position: absolute;top: 2100px;">
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<img src="https://static.igem.org/mediawiki/2018/0/00/T--Jiangnan--main--susmove.png" width="100%">
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<div style="position: absolute;top: 2200px; left: 40px">
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</div>
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<img src="https://static.igem.org/mediawiki/2018/d/dd/T--Jiangnan--main--sustitle.png" width="300">
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<div class="col s7">
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<br>
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<h5 style="text-align: right;color: #ff9100">Suspension Culture</h5>
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<br>
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<p>Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.</p>
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<p style="width: 700px;">Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation. </p>
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</div>
 
</div>
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<img id="susmove" src="https://static.igem.org/mediawiki/2018/0/00/T--Jiangnan--main--susmove.png" width="400" style="position: absolute;top: 2200px;right: 40px;">
 
 
</div>
 
</div>
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<div id="broadspectrum" class="scrollspy">
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<div id="high" class="row">
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<div style="color:white;width: 100%;height: 700px"></div>
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<div class="col s7 offset-s1">
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<img src="https://static.igem.org/mediawiki/2018/0/0b/T--Jiangnan--main--broad_specmforw.png" width="100%" height="700" style="position: absolute;top: 2800px;">
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<h5 style="color: #03a9f4">High Titer</h5>
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<div style="position: absolute;top: 3000px;left: 40px">
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<p>Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titer of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titer. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titer was further increased in response to plasma irradiation.</p>
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<p style="width: 700px;">Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.
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        <li><a href="#high_titer">High titration</a></li>
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Latest revision as of 03:01, 18 October 2018

Safety
Hardware
Team
Human Practice
Model
Notebook
Project

Project Description

The limiting factors of most vaccine companies, according to our previous investigation and survey, are mulitifaceted costs in actual production process and long product lift cycle and other issues due to complex application process of new vaccines(cell line).
The goal of our project is to enable three features of our chassis cell, i.e.,high titer, suspension cultivation, and broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here is MDBK cell.

Broad Spectrum

Suspension Culture

High Titer

Broad Spectrum

Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.

Suspension Culture

Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.

High Titer

Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titer of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titer. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titer was further increased in response to plasma irradiation.