Difference between revisions of "Team:Jiangnan"
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<link rel="stylesheet" href="https://2018.igem.org/Team:Jiangnan/CSSmaterializecss?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2018.igem.org/Team:Jiangnan/CSSmaterializecss?action=raw&ctype=text/css"> | ||
| + | <link rel="stylesheet" href="https://2018.igem.org/Team:Jiangnan/CSSanimatecss?action=raw&ctype=text/css"> | ||
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<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Gold</a></li> | <li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Gold</a></li> | ||
<li class="divider"></li> | <li class="divider"></li> | ||
| − | <li><a href="https://2018.igem.org/Team:Jiangnan/Public_Engagement"> | + | <li><a href="https://2018.igem.org/Team:Jiangnan/Public_Engagement">Public Engagement</a></li> |
<li class="divider"></li> | <li class="divider"></li> | ||
<li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Entrepreneurship</a></li> | <li><a href="https://2018.igem.org/Team:Jiangnan/Entrepreneurship">Entrepreneurship</a></li> | ||
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| − | <div style="width:100%; | + | <div style="width:100%;"> |
<div style="text-align: center;"> | <div style="text-align: center;"> | ||
<video class="responsive-video" controls> | <video class="responsive-video" controls> | ||
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<img src="https://static.igem.org/mediawiki/2018/a/a1/T--Jiangnan--main_logo.png" width="10%"> | <img src="https://static.igem.org/mediawiki/2018/a/a1/T--Jiangnan--main_logo.png" width="10%"> | ||
<h4 style="color: white;">Project Description</h4> | <h4 style="color: white;">Project Description</h4> | ||
| − | <div style="margin-bottom: 0; width: 60%;display: inline;position: relative;"> | + | <div style="margin-bottom: 0; width: 60%; display: inline; position: relative;"> |
| − | <p style="text-align: center;color: white">The | + | <p style="text-align: center; color: white;">The limiting factors of most vaccine companies, according to our previous investigation and survey, are |
| − | mulitifaceted costs in actual production process and long product lift cycle and other issues due | + | mulitifaceted costs in actual production process and long product lift cycle and other issues due |
| − | to complex application process of new vaccines( | + | to complex application process of new vaccines(cell line).<br/> |
| − | The goal of our project is to enable three features of our chassis cell, i.e.,high | + | The goal of our project is to enable three features of our chassis cell, i.e.,high titer, suspension cultivation, and |
| − | broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here | + | broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here is |
| − | + | MDBK cell.</p> | |
</div> | </div> | ||
<div style="width: 33.3%;float: left;"> | <div style="width: 33.3%;float: left;"> | ||
<a href="#broad"><img class="animated infinite pulse" src="https://static.igem.org/mediawiki/2018/0/06/T--JiangNan--broad.png" width="50%"></a> | <a href="#broad"><img class="animated infinite pulse" src="https://static.igem.org/mediawiki/2018/0/06/T--JiangNan--broad.png" width="50%"></a> | ||
| − | <div><h4 style="color: white">Broad | + | <div><h4 style="color: white">Broad Spectrum</h4></div> |
</div> | </div> | ||
<div style="width: 33.3%;float: left;"> | <div style="width: 33.3%;float: left;"> | ||
<a href="#sus"><img class="animated infinite swing" src="https://static.igem.org/mediawiki/2018/c/cf/T--Jiangnan--main--sus.png" width="50%"></a> | <a href="#sus"><img class="animated infinite swing" src="https://static.igem.org/mediawiki/2018/c/cf/T--Jiangnan--main--sus.png" width="50%"></a> | ||
| − | <div><h4 style="color: white">Suspension</h4></div> | + | <div><h4 style="color: white">Suspension Culture</h4></div> |
</div> | </div> | ||
<div style="width: 33.3%;float: left;"> | <div style="width: 33.3%;float: left;"> | ||
<a href="#high"><img class="animated infinite rubberBand" src="https://static.igem.org/mediawiki/2018/9/96/T--Jiangnan--main--high.png" width="50%"></a> | <a href="#high"><img class="animated infinite rubberBand" src="https://static.igem.org/mediawiki/2018/9/96/T--Jiangnan--main--high.png" width="50%"></a> | ||
| − | <div><h4 style="color: white">High | + | <div><h4 style="color: white">High Titer</h4></div> |
</div> | </div> | ||
</div> | </div> | ||
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<div id="broad" class="row"> | <div id="broad" class="row"> | ||
<div class="col s7 offset-s1"> | <div class="col s7 offset-s1"> | ||
| − | <h5 style="color: #2e7d32">Broad | + | <h5 style="color: #2e7d32">Broad Spectrum</h5> |
<p>Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.</p> | <p>Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.</p> | ||
</div> | </div> | ||
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<div class="col s7"> | <div class="col s7"> | ||
| − | <h5 style="text-align: right;color: #ff9100">Suspension</h5> | + | <h5 style="text-align: right;color: #ff9100">Suspension Culture</h5> |
<p>Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.</p> | <p>Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.</p> | ||
</div> | </div> | ||
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<div id="high" class="row"> | <div id="high" class="row"> | ||
<div class="col s7 offset-s1"> | <div class="col s7 offset-s1"> | ||
| − | <h5 style="color: #03a9f4">High | + | <h5 style="color: #03a9f4">High Titer</h5> |
| − | <p>Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus | + | <p>Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titer of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titer. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titer was further increased in response to plasma irradiation.</p> |
</div> | </div> | ||
<div class="col s3"> | <div class="col s3"> | ||
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</div> | </div> | ||
</div> | </div> | ||
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<img src="https://static.igem.org/mediawiki/2018/b/b6/T--Jiangnan--thanks.png" width="100%" style="margin-bottom: -1em;"> | <img src="https://static.igem.org/mediawiki/2018/b/b6/T--Jiangnan--thanks.png" width="100%" style="margin-bottom: -1em;"> | ||
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Latest revision as of 03:01, 18 October 2018
Project Description
The limiting factors of most vaccine companies, according to our previous investigation and survey, are
mulitifaceted costs in actual production process and long product lift cycle and other issues due
to complex application process of new vaccines(cell line).
The goal of our project is to enable three features of our chassis cell, i.e.,high titer, suspension cultivation, and
broad spectrum to reduce the production cost increase the yield of viruses for vaccine production. The chassis cell we used here is
MDBK cell.
Broad Spectrum
Suspension Culture
High Titer
Broad Spectrum
Through text mining, we classified cell receptors according to the Baltimore subtyping of viruses and summarized the primary receptors mediating the entry of different types of viruses. After systematic analysis, we aim to express Nectin 4 and Tfr on our chassis cells to make them possible to a broad spectrum of viruses.
Suspension Culture
Through high throughput sequencing of two pairs of cells with and without the suspension feature, we found a panel of genes responsible for the suspension feature of cells following network construction using computational approach. The top gene was functionally validated before applied to the chassis cell for genomic modulation.
High Titer
Through bioinformatics analysis and mathematical modeling, we constructed the network regulating virus titer of cells. The top gene was selected, functionally validated in vitro and used for genomic modulation in the chassis cells to enable them the feature of increased titer. On the other hand, we manufactured a device to generate cold atmospheric plasma, using which cell titer was further increased in response to plasma irradiation.
