Difference between revisions of "Team:H14Z1 Hangzhou"

 
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{{H14Z1_Hangzhou}}
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<h1> Welcome to iGEM 2018! </h1>
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            <img src="https://static.igem.org/mediawiki/2018/4/45/T--H14Z1_Hangzhou--head_home.png" alt="" class="head_div_img" />
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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                <h3 class="content_subtitle" style="text-align:center">Title</h3>
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                    Smart Yogurt：A possible alternative supply of two liver-protective factors with
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                    colonization-prone <i>Lactococcus lactisa</i>
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                </p>
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                <h3 class="content_subtitle" style="text-align:center">Abstract</h3>
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                    A variety of liver diseases occur throughout the world irrespective of age, sex, region or
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                    race. Two important liver-protective factors (glutathione and S-adenosyl methionine) has been
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                    applied to prevent and treat many different liver diseases and damage. Due to low stability and
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                    short half-life, oral table supply of GSH or SAM might be replaced by a novel strategy of
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                    supplying these molecules continuously using our synthetic biology design of smart yogurt. In
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                    the experiment, two-functional GSH synthetase gene (<i>gshF</i>) and SAM synthetase gene (<i>metK</i>) were
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                    in tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ-GM) was
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                    further employed to construct the target vector pNZ-GMcA by introducing adhesion factor gene
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                    (<i>cwaA</i>). This target vector was confirmed by gene sequencing and transformed into food-grade
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                    <i>Lactococcus lactis</i>. Further studies showed that the synthetic levels of SAM and GSH were
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                    improved greatly with <i>Lactococcus lactis</i> NZ9000/pNZ-GMcA, and the colonization-prone ability
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                    was also increased simultaneously. Finally, this recombinant <i>Lactococcus lactis</i> was inoculated
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                    to produce our smart yogurt separately or combined with <i>Lactobacillus delbruecki</i>. The present
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                    work paved one road to supply liver-protective agents using smart yogurt concept thanks to
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                    synthetic biology.
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Latest revision as of 03:04, 18 October 2018

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Title

Smart Yogurt：A possible alternative supply of two liver-protective factors with colonization-prone Lactococcus lactisa

Abstract

A variety of liver diseases occur throughout the world irrespective of age, sex, region or race. Two important liver-protective factors (glutathione and S-adenosyl methionine) has been applied to prevent and treat many different liver diseases and damage. Due to low stability and short half-life, oral table supply of GSH or SAM might be replaced by a novel strategy of supplying these molecules continuously using our synthetic biology design of smart yogurt. In the experiment, two-functional GSH synthetase gene (gshF) and SAM synthetase gene (metK) were in tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ-GM) was further employed to construct the target vector pNZ-GMcA by introducing adhesion factor gene (cwaA). This target vector was confirmed by gene sequencing and transformed into food-grade Lactococcus lactis. Further studies showed that the synthetic levels of SAM and GSH were improved greatly with Lactococcus lactis NZ9000/pNZ-GMcA, and the colonization-prone ability was also increased simultaneously. Finally, this recombinant Lactococcus lactis was inoculated to produce our smart yogurt separately or combined with Lactobacillus delbruecki. The present work paved one road to supply liver-protective agents using smart yogurt concept thanks to synthetic biology.