Difference between revisions of "Team:H14Z1 Hangzhou"

 
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            <div class="content_conts" style="text-align:center">
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            <h1>Lorem Ipsum 1</h1>
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                    <img class="slideshow_picture" style="margin:0 auto" src="https://static.igem.org/mediawiki/2018/4/4f/T--H14Z1_Hangzhou--home_first.jpg">
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                <p>Phage text</p>
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                    <img class="slideshow_picture" src="https://static.igem.org/mediawiki/2018/9/96/T--H14Z1_Hangzhou--home_four.jpg">
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                <b>Lorem ipsum dolor sit amet, consectetur adipiscing elit.</b> Morbi et faucibus leo. Nullam gravida nibh
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                    <h1>Lorem Ipsum 4</h1>
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                        <p>Black IGEM text</p>
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                        Nullam in hendrerit lacus. Interdum et malesuada fames ac ante ipsum primis in faucibus. Aliquam
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                    Hide
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            <div class="content_box" style="margin-top:100px">
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                <h3 class="content_subtitle" style="text-align:center">Title</h3>
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                    Smart Yogurt：A possible alternative supply of two liver-protective factors with
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                    colonization-prone <i>Lactococcus lactisa</i>
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        <div class="paragraph_img_left">
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                 <h3 class="content_subtitle" style="text-align:center">Abstract</h3>
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            <h1>Lorem Ipsum 2</h1>
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                <p class="content_context" style="line-height:32px; font-size: 18px; text-align: justify; text-indent: 2em">
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            <div>
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                    A variety of liver diseases occur throughout the world irrespective of age, sex, region or
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                 <img src="https://static.igem.org/mediawiki/2017/d/d7/T--NTNU_Trondheim--Phage_under_construction.png">
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                    race. Two important liver-protective factors (glutathione and S-adenosyl methionine) has been
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                <p>Standard IGEM text</p>
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                    applied to prevent and treat many different liver diseases and damage. Due to low stability and
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                    short half-life, oral table supply of GSH or SAM might be replaced by a novel strategy of
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                    supplying these molecules continuously using our synthetic biology design of smart yogurt. In
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                    the experiment, two-functional GSH synthetase gene (<i>gshF</i>) and SAM synthetase gene (<i>metK</i>) were
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                    in tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ-GM) was
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                    further employed to construct the target vector pNZ-GMcA by introducing adhesion factor gene
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                    (<i>cwaA</i>). This target vector was confirmed by gene sequencing and transformed into food-grade
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                    <i>Lactococcus lactis</i>. Further studies showed that the synthetic levels of SAM and GSH were
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                    improved greatly with <i>Lactococcus lactis</i> NZ9000/pNZ-GMcA, and the colonization-prone ability
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                    was also increased simultaneously. Finally, this recombinant <i>Lactococcus lactis</i> was inoculated
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                    to produce our smart yogurt separately or combined with <i>Lactobacillus delbruecki</i>. The present
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                    work paved one road to supply liver-protective agents using smart yogurt concept thanks to
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                    synthetic biology.
 +
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                <sup>Vestibulum facilisis aliquam accumsan. Phasellus facilisis lacinia dapibus.</sup> Ut dictum purus sed risus
 
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                porta lobortis. Suspendisse maximus posuere commodo. Duis pharetra sit amet sapien in convallis. Proin
 
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                ultricies luctus massa. Morbi tristique turpis eget turpis vulputate, a scelerisque magna scelerisque.
 
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                amet. Sed sit amet nunc sagittis, tincidunt dui ac, euismod sem. Class aptent taciti sociosqu ad litora
 
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                torquent per conubia nostra, per inceptos himenaeos. Praesent convallis mollis semper. Praesent accumsan
 
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                massa sed nulla faucibus, laoreet faucibus ante vestibulum. Nulla id enim a felis porttitor fermentum
 
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                nec ac velit. Etiam dapibus molestie turpis a sodales.
 
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        <div class="image" style="width: 50%;margin-top: 5em">
 
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            <!-- The width of the page the picture will occupy. The height is calculated from it to honor the source dimensions. Should NOT exceed 70%-->
 
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            <img src="https://static.igem.org/mediawiki/2017/1/1f/T--NTNU_Trondheim--Jump_with_Sheida.jpg">
 
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            <p>Jump text</p>
 
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            <h1>Lorem Ipsum 3</h1>
 
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            <p>
 
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                Duis porta pulvinar orci ac accumsan. Curabitur sagittis risus nibh, nec sodales velit posuere id. Sed
 
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                eu lobortis orci, in sodales ligula. Sed vel diam vel ante tempus viverra eu et turpis. Aenean porta,
 
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Latest revision as of 03:04, 18 October 2018

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Title

Smart Yogurt：A possible alternative supply of two liver-protective factors with colonization-prone Lactococcus lactisa

Abstract

A variety of liver diseases occur throughout the world irrespective of age, sex, region or race. Two important liver-protective factors (glutathione and S-adenosyl methionine) has been applied to prevent and treat many different liver diseases and damage. Due to low stability and short half-life, oral table supply of GSH or SAM might be replaced by a novel strategy of supplying these molecules continuously using our synthetic biology design of smart yogurt. In the experiment, two-functional GSH synthetase gene (gshF) and SAM synthetase gene (metK) were in tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ-GM) was further employed to construct the target vector pNZ-GMcA by introducing adhesion factor gene (cwaA). This target vector was confirmed by gene sequencing and transformed into food-grade Lactococcus lactis. Further studies showed that the synthetic levels of SAM and GSH were improved greatly with Lactococcus lactis NZ9000/pNZ-GMcA, and the colonization-prone ability was also increased simultaneously. Finally, this recombinant Lactococcus lactis was inoculated to produce our smart yogurt separately or combined with Lactobacillus delbruecki. The present work paved one road to supply liver-protective agents using smart yogurt concept thanks to synthetic biology.