Difference between revisions of "Team:H14Z1 Hangzhou"

 
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            <h1>Project Phage Age</h1>
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                 Team NTNU Trondheim 2017 is a team of 8 students from the Norwegian University of Science and Technology
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                 <h3 class="content_subtitle" style="text-align:center">Title</h3>
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                (NTNU) participating in the <a href="https://igem.org/Main_Page">iGEM Competition</a>: the world’s
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                 <p class="content_context" style="line-height:32px; font-size: 18px; text-align: justify">
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                 biggest student
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                    Smart Yogurt：A possible alternative supply of two liver-protective factors with
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                team competition in synthetic biology. <a href="https://2017.igem.org/Team:NTNU_Trondheim/Team">We</a>
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                    colonization-prone <i>Lactococcus lactisa</i>
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                 are glad to present <b>Project Phage Age</b>!
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                 </p>
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                 <br><br>
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                 <h3 class="content_subtitle" style="text-align:center">Abstract</h3>
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                 With our project we aim to face the approaching challenge of antibiotics resistance by taking a
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                 <p class="content_context" style="line-height:32px; font-size: 18px; text-align: justify; text-indent: 2em">
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                closer look into the exciting area of phage-therapy. We have developed a platform for evolving
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                    A variety of liver diseases occur throughout the world irrespective of age, sex, region or
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                bacteriophages into killing specific, target bacteria that were previously resistant towards the phage.
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                    race. Two important liver-protective factors (glutathione and S-adenosyl methionine) has been
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                As bacterial antibiotics resistance mechanisms does not affect bacteriophages, we believe that this
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                    applied to prevent and treat many different liver diseases and damage. Due to low stability and
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                approach can be a promising contribution in the quest for battling multi-antibioticresistant bacterial
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                    short half-life, oral table supply of GSH or SAM might be replaced by a novel strategy of
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                infections.
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                    supplying these molecules continuously using our synthetic biology design of smart yogurt. In
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                    the experiment, two-functional GSH synthetase gene (<i>gshF</i>) and SAM synthetase gene (<i>metK</i>) were
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            </p>
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                    in tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ-GM) was
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        </div>
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                    further employed to construct the target vector pNZ-GMcA by introducing adhesion factor gene
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                    (<i>cwaA</i>). This target vector was confirmed by gene sequencing and transformed into food-grade
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        <div class="break_2">
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                    <i>Lactococcus lactis</i>. Further studies showed that the synthetic levels of SAM and GSH were
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            <img src="https://static.igem.org/mediawiki/2017/5/52/T--NTNU_Trondheim--splitter_2.svg">
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                    improved greatly with <i>Lactococcus lactis</i> NZ9000/pNZ-GMcA, and the colonization-prone ability
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        </div>
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                    was also increased simultaneously. Finally, this recombinant <i>Lactococcus lactis</i> was inoculated
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                    to produce our smart yogurt separately or combined with <i>Lactobacillus delbruecki</i>. The present
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        <div class="paragraph_img_left">
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                    work paved one road to supply liver-protective agents using smart yogurt concept thanks to
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                    synthetic biology.
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                 <img src="https://static.igem.org/mediawiki/2017/1/1d/T--NTNU_Trondheim--Phage.png">
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                 </p>
 
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            <h1 style="width: 60% !important;">Read more about...</h1>
 
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                <br>
 
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                <a href="https://2017.igem.org/Team:NTNU_Trondheim/Background">The problem with antibiotics
 
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                    resistance</a><br><br>
 
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                <a href="https://2017.igem.org/Team:NTNU_Trondheim/Description">Our approach to solving the
 
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                    problem</a><br><br>
 
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                <a href="https://2017.igem.org/Team:NTNU_Trondheim/Hardware">The hardware platform that we
 
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                    developed</a><br><br>
 
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                <a href="https://2017.igem.org/Team:NTNU_Trondheim/Results">The results of our project</a><br><br>
 
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            </p>
 
 
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            <img src="https://static.igem.org/mediawiki/2017/1/1f/T--NTNU_Trondheim--Jump_with_Sheida.jpg">
 
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            <p>Our team, leaping for the sky.</p>
 
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            <h1>Thank you!</h1>
 
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            <p>
 
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                We would like to thank all our sponsors without which we would never have been able to complete our
 
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                project. They have made very important economic contributions, and have also showed both interest and
 
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                support. Special thanks go to the Norwegian University of Science and Technology (NTNU) that supplied
 
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                us with supervisors, as well as providing us with our own lab space and basic equipment. At last we
 
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                would like to thank everyone who helped and supported our project so that we were able to finish it
 
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                and present it at the Giant Jamboree in Boston in November 2017. See <a
 
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                    href="https://2017.igem.org/Team:NTNU_Trondheim/Attributions">Attributions</a>,
 
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                <a href="https://2017.igem.org/Team:NTNU_Trondheim/People/Sponsors">Sponsors</a> and
 
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                <a href="https://2017.igem.org/Team:NTNU_Trondheim/Collaborations">Collaboration</a> to read more about
 
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                the different people and companies who helped and supported our project.
 
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Latest revision as of 03:04, 18 October 2018

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Title

Smart Yogurt：A possible alternative supply of two liver-protective factors with colonization-prone Lactococcus lactisa

Abstract

A variety of liver diseases occur throughout the world irrespective of age, sex, region or race. Two important liver-protective factors (glutathione and S-adenosyl methionine) has been applied to prevent and treat many different liver diseases and damage. Due to low stability and short half-life, oral table supply of GSH or SAM might be replaced by a novel strategy of supplying these molecules continuously using our synthetic biology design of smart yogurt. In the experiment, two-functional GSH synthetase gene (gshF) and SAM synthetase gene (metK) were in tandem inserted into the expression vector (pNZ8148), and the resulted plasmid (pNZ-GM) was further employed to construct the target vector pNZ-GMcA by introducing adhesion factor gene (cwaA). This target vector was confirmed by gene sequencing and transformed into food-grade Lactococcus lactis. Further studies showed that the synthetic levels of SAM and GSH were improved greatly with Lactococcus lactis NZ9000/pNZ-GMcA, and the colonization-prone ability was also increased simultaneously. Finally, this recombinant Lactococcus lactis was inoculated to produce our smart yogurt separately or combined with Lactobacillus delbruecki. The present work paved one road to supply liver-protective agents using smart yogurt concept thanks to synthetic biology.