Difference between revisions of "Team:Jiangnan China"

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{{Jiangnan_China}}
 
{{Jiangnan_China}}
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    <nav class="site-header py-2" style="position: fixed;width: 100%;z-index: 999">
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      <div class="container d-flex flex-column flex-md-row justify-content-between" style="max-width: 1300px;">
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        <a class="navbar-brand" href="#">
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          <img src="https://static.igem.org/mediawiki/2018/8/84/T--jiangnan_china--home--icon-logo.png" width="36px" height="36px">
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        </a>
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        <a class="nav-link py-2 d-none d-md-inline-block" href="https://2018.igem.org/Team:Jiangnan_China"><i class="fa fa-home"></i> Home</a>
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        <div class="dropdown">
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          <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
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            <i class="fa fa-group"></i> Team
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          </a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/TeamMembers">Team Members</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Description">Description</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Design">Design</a>
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Experiments">Experiments</a>
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            <i class="fa fa-bell"></i> Safety
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          <div class="dropdown-menu" aria-labelledby="projectDropdown">
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Safety">Training</a>
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            <i class="fa fa-part"></i> Parts
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            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Parts">Basic Parts</a>
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          </div>
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        </div>
  
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        <a class="nav-link py-2 d-none d-md-inline-block" href="https://2018.igem.org/Team:Jiangnan_China/InterLab"><i class="fa fa-interlab" aria-hidden="true"></i> InterLab</a>
  
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<h1> Welcome to iGEM 2018! </h1>
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      <img src="https://static.igem.org/mediawiki/2018/3/35/T--jiangnan_china--wzh.jpg" width="100%">
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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      <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
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        <!-- <div align="center" style="font-family: 'spr';font-size:40px;"><strong>Summary</strong></div> -->
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        <br>
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      &nbsp;&nbsp;&nbsp;&nbsp;Lactic acid bacteria are the most promising microorganisms to act as live vaccines as well as microbial cell factory which can produce various chemicals. During fermentation processing, they suffer from various stress conditions, especially acid and cold stress. Therefore, we aim to develop an ideal food-safe grade microorganism with enhanced acid and cold tolerance.<br>
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      &nbsp;&nbsp;&nbsp;&nbsp;Genome mutagenesis combined with high-throughput technologies was performed on <span class="font-italic">Lactococcus lactis</span> NZ9000 to screen acid tolerance strain. Next, comparative transcriptomics analysis was performed on mutant and parent strain to investigate the response mechanisms of microbial cells during acid stress. Based on the proposed acid tolerance mechanisms, one new anti-acid component—<span class="font-italic">msmK</span> was discovered. Also, an anti-cold gene <span class="font-italic">cspD2</span> was selected. The constructed recombinant strain <span class="font-italic">Lactococcus lactis</span> NZ3900/pNZ 8149-<span class="font-italic">msmK-cspD2</span> shows a significant survival advantage compared with <span class="font-italic">Lactococcus lactis</span> NZ3900/pNZ 8149, which means our product exhibited enhanced acid and cold tolerance.<br>
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      &nbsp;&nbsp;&nbsp;&nbsp;This study provides valuable insight into the development of robust industrial strains.<br>
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      <br><br>
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      <p style="text-align: center;"><a href="https://2018.igem.org/Team:Jiangnan_China/Description" class="btn btn-info">Click here for more details. </a></p>
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<h3>Before you start</h3>
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      <video controls="controls" src="https://static.igem.org/mediawiki/2018/3/3c/T--jiangnan_china--intro1.mp4" width="75%" style="margin-left:12.5%; margin-top:50px; border:5px solid #000000;">
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<p> Please read the following pages:</p>
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      </video>
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<li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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        <a href="http://www.jiangnan.edu.cn/" title="江南大学"><img src="https://static.igem.org/mediawiki/2018/1/1d/T--jiangnan_china--jiangnanlogo.png"></a>
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        <a href="http://biotech.jiangnan.edu.cn/" title="江南大学生物工程学院"><img src="https://static.igem.org/mediawiki/2018/a/a6/T--jiangnan_china--shenggonglogo.png"></a>
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        <p>Copyright &copy; jiangnan_China 2018</p>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Latest revision as of 03:06, 18 October 2018


    Lactic acid bacteria are the most promising microorganisms to act as live vaccines as well as microbial cell factory which can produce various chemicals. During fermentation processing, they suffer from various stress conditions, especially acid and cold stress. Therefore, we aim to develop an ideal food-safe grade microorganism with enhanced acid and cold tolerance.
    Genome mutagenesis combined with high-throughput technologies was performed on Lactococcus lactis NZ9000 to screen acid tolerance strain. Next, comparative transcriptomics analysis was performed on mutant and parent strain to investigate the response mechanisms of microbial cells during acid stress. Based on the proposed acid tolerance mechanisms, one new anti-acid component—msmK was discovered. Also, an anti-cold gene cspD2 was selected. The constructed recombinant strain Lactococcus lactis NZ3900/pNZ 8149-msmK-cspD2 shows a significant survival advantage compared with Lactococcus lactis NZ3900/pNZ 8149, which means our product exhibited enhanced acid and cold tolerance.
    This study provides valuable insight into the development of robust industrial strains.


Click here for more details.

The video below intends to introduce the adventurer trip.

Copyright © jiangnan_China 2018