Difference between revisions of "Team:Jiangnan China"

Latest revision as of 03:06, 18 October 2018

    Lactic acid bacteria are the most promising microorganisms to act as live vaccines as well as microbial cell factory which can produce various chemicals. During fermentation processing, they suffer from various stress conditions, especially acid and cold stress. Therefore, we aim to develop an ideal food-safe grade microorganism with enhanced acid and cold tolerance.
    Genome mutagenesis combined with high-throughput technologies was performed on Lactococcus lactis NZ9000 to screen acid tolerance strain. Next, comparative transcriptomics analysis was performed on mutant and parent strain to investigate the response mechanisms of microbial cells during acid stress. Based on the proposed acid tolerance mechanisms, one new anti-acid component—msmK was discovered. Also, an anti-cold gene cspD2 was selected. The constructed recombinant strain Lactococcus lactis NZ3900/pNZ 8149-msmK-cspD2 shows a significant survival advantage compared with Lactococcus lactis NZ3900/pNZ 8149, which means our product exhibited enhanced acid and cold tolerance.
    This study provides valuable insight into the development of robust industrial strains.

Click here for more details.

The video below intends to introduce the adventurer trip.

@@ Line 1: / Line 1: @@
 {{Jiangnan_China}}
 <html lang="en">
    <body style="background-color: #ccc">
      <nav class="site-header py-2" style="position: fixed;width: 100%;z-index: 999">
@@ Line 7: / Line 8: @@
            <img src="https://static.igem.org/mediawiki/2018/8/84/T--jiangnan_china--home--icon-logo.png" width="36px" height="36px">
          </a>
-         <a class="nav-link py-2 d-none d-md-inline-block" href="#"><i class="fa fa-home"></i> Home</a>
+         <a class="nav-link py-2 d-none d-md-inline-block" href="https://2018.igem.org/Team:Jiangnan_China"><i class="fa fa-home"></i> Home</a>
          <div class="dropdown">
            <a class="nav-link dropdown-toggle" href="#" id="navbarDropdown" role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
@@ Line 13: / Line 14: @@
            </a>
            <div class="dropdown-menu" aria-labelledby="navbarDropdown">
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/TeamMembers">Team Members</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/TeamMembers">Team Members</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Sponsors">Sponsors</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Sponsors">Sponsors</a>
              <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Attributions">Attributions</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Collaborations">Collaborations</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Collaborations">Collaborations</a>
            </div>
          </div>
@@ Line 24: / Line 25: @@
            </a>
            <div class="dropdown-menu" aria-labelledby="projectDropdown">
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Description">Description</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Description">Description</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Design">Design</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Design">Design</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Experiments">Experiments</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Experiments">Experiments</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Notebook">Notebook</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Notebook">Notebook</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Protocols">Protocols</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Protocols">Protocols</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Results">Results</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Results">Results</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Demonstrate">Demonstrate</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Demonstrate">Demonstrate</a>
+            <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Model">Model</a>
            </div>
          </div>
@@ Line 38: / Line 40: @@
            </a>
            <div class="dropdown-menu" aria-labelledby="projectDropdown">
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Training">Training</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Safety">Training</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Protection">Protection</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Safety#Protection">Protection</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Material">Material</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Safety#Material">Material</a>
            </div>
          </div>
@@ Line 48: / Line 50: @@
            </a>
            <div class="dropdown-menu" aria-labelledby="partsDropdown">
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Overview">Overview</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Human_Practices">Silver_Human Pactices</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Socialresearch">Social Research</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Human_Practices#gold">Gold_Human Pactices</a>
-            <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Education">Education</a>
            </div>
          </div>
@@ Line 58: / Line 59: @@
            </a>
            <div class="dropdown-menu" aria-labelledby="partsDropdown">
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Bronzes">Bronzes</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Awards">Bronzes</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Silver">Silver</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Awards#Silver">Silver</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Gold">Gold</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Awards#Gold">Gold</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Prizes">Prizes</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Awards#Prizes">Prizes</a>
            </div>
          </div>
@@ Line 69: / Line 70: @@
            </a>
            <div class="dropdown-menu" aria-labelledby="partsDropdown">
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/BasicParts">Basic Parts</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Parts">Basic Parts</a>
-             <a class="dropdown-item" href="https://2018.igem.org/Team:jiangnan_China/Compositeparts">Composite parts</a>
+             <a class="dropdown-item" href="https://2018.igem.org/Team:Jiangnan_China/Parts#Composite">Composite parts</a>
            </div>
          </div>
-         <a class="nav-link py-2 d-none d-md-inline-block" href="https://2018.igem.org/Team:jiangnan_China/interlab"><i class="fa fa-interlab" aria-hidden="true"></i> Interlab</a>
+         <a class="nav-link py-2 d-none d-md-inline-block" href="https://2018.igem.org/Team:Jiangnan_China/InterLab"><i class="fa fa-interlab" aria-hidden="true"></i> InterLab</a>
        </div>
@@ Line 80: / Line 81: @@
      <main class="content-wrap">
-       <img src="https://static.igem.org/mediawiki/2018/6/68/T--jiangnan_china--home--2.jpg" width="100%">
+       <img src="https://static.igem.org/mediawiki/2018/3/35/T--jiangnan_china--wzh.jpg" width="100%">
        <div class="dcpt3" style="font-size:20px;line-height:1.5;font-family: 'spr';">
-     <div align="center" style="font-family: 'spr';font-size:40px;"><strong>Summary</strong></div>
+        <!-- <div align="center" style="font-family: 'spr';font-size:40px;"><strong>Summary</strong></div> -->
-       &nbsp;&nbsp;&nbsp;&nbsp;Aiming at creating an ideal chassis microorganism with good resistance ability and food-safe characteristic by synthetic biology methods. We added four components to model strain Lactococcus lactis NZ3900 to afforded it the anti-acid, anti-cold characteristics.
+        <br>
+       &nbsp;&nbsp;&nbsp;&nbsp;Lactic acid bacteria are the most promising microorganisms to act as live vaccines as well as microbial cell factory which can produce various chemicals. During fermentation processing, they suffer from various stress conditions, especially acid and cold stress. Therefore, we aim to develop an ideal food-safe grade microorganism with enhanced acid and cold tolerance.<br>
+      &nbsp;&nbsp;&nbsp;&nbsp;Genome mutagenesis combined with high-throughput technologies was performed on <span class="font-italic">Lactococcus lactis</span> NZ9000 to screen acid tolerance strain. Next, comparative transcriptomics analysis was performed on mutant and parent strain to investigate the response mechanisms of microbial cells during acid stress. Based on the proposed acid tolerance mechanisms, one new anti-acid component—<span class="font-italic">msmK</span> was discovered. Also, an anti-cold gene <span class="font-italic">cspD2</span> was selected. The constructed recombinant strain <span class="font-italic">Lactococcus lactis</span> NZ3900/pNZ 8149-<span class="font-italic">msmK-cspD2</span> shows a significant survival advantage compared with <span class="font-italic">Lactococcus lactis</span> NZ3900/pNZ 8149, which means our product exhibited enhanced acid and cold tolerance.<br>
+      &nbsp;&nbsp;&nbsp;&nbsp;This study provides valuable insight into the development of robust industrial strains.<br>
        <br><br>
-       <p style="text-align: center;"><a href="https://2018.igem.org/Team:jiangnan_China/Description" class="btn btn-info">Click here for more details. </a></p>
+       <p style="text-align: center;"><a href="https://2018.igem.org/Team:Jiangnan_China/Description" class="btn btn-info">Click here for more details. </a></p>
        </div>
+<div style="background-color: #fffae6;">
        <div style="font-size:28px;line-height:300%;font-weight:bold;margin:0 auto;margin-top:-10px; margin-bottom:-25px;color:#f75000;text-align:center; font-family:fsb;">The video below intends to introduce the adventurer trip.</div>
        <video controls="controls" src="https://static.igem.org/mediawiki/2018/3/3c/T--jiangnan_china--intro1.mp4" width="75%" style="margin-left:12.5%; margin-top:50px; border:5px solid #000000;">
         your browser don't support html5.
         </video>
+</div>
      </main>