Difference between revisions of "Team:East Chapel Hill/Human Practices"

 
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  <a href="#Introduction" class="active">Introduction</a>
 
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  <a href="#Solution">Solution</a>
 
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<h1>Description</h1>
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<h1>Human Practices</h1>
 
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<h1>Introduction</h1>
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<h2> Human Practices Framework </h2>
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In nature, the riboswitch regulates the expression of genes that help the organism deal with high levels of fluoride. These genes are often pumps that allow fluoride to be exported out of the cell (<b>Figure 4</b>). In <i>E. coli</i> the gene crcB encodes a fluoride efflux channel that removes excess fluoride from the cell so that it is no longer toxic. In <i>E. coli</i> when the crcB gene is genetically deleted (ΔcrcB), the phenotype is increased sensitivity to fluoride and concentrations above 500μM are lethal. In our experiments we needed to utilize the ΔcrcB <i>E. coli</i> strain so that fluoride could accumulate intracellularly.
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<br>
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1. Identifying the Problem
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<br> - <a href="https://2018.igem.org/Team:East_Chapel_Hill/MaikoSuzuki"><b><u>Interview with Maiko Suzuki from Ohio University regarding fluorosis. </u></b></a>
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<br><br>
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2. Assessing Current Technologies
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<br> - Interview with Tewodros Godebo, an in-field fluoride management expert.
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<br><br>
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3. Understanding Public Perception
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<br> - <a href="https://2018.igem.org/Team:East_Chapel_Hill/Collaborations"><b><u>Public forum held at Duke University. </u></b></a>
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<br> - <a href="https://2018.igem.org/Team:East_Chapel_Hill/Public_Engagement"><b><u>Fundraisers at the Chapel Hill Public Library.</u></b></a>
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<br> - <a href="https://2018.igem.org/Team:East_Chapel_Hill/Public_Engagement"><b><u>Education and Engagement</u></b></a>
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<br> - <a href="https://2018.igem.org/Team:East_Chapel_Hill/SchoolActivitise"><b><u>CRISPR worksheet for high schoolers and Presentation for Advanced Placement Environmental Science classes</u></b></a>
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<br> - Activities at KidZu Museum and Life and Sciences Museum
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<br> - <a href="https://2018.igem.org/Team:East_Chapel_Hill/MaikoSuzuki"><b><u>Short educational Youtube video. </u></b></a>
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<h1>References</h1>
 
  
  
 
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Latest revision as of 03:11, 18 October 2018


East Chapel Hill

Human Practices


Human Practices Framework


1. Identifying the Problem
- Interview with Maiko Suzuki from Ohio University regarding fluorosis.

2. Assessing Current Technologies
- Interview with Tewodros Godebo, an in-field fluoride management expert.

3. Understanding Public Perception
- Public forum held at Duke University.
- Fundraisers at the Chapel Hill Public Library.
- Education and Engagement
- CRISPR worksheet for high schoolers and Presentation for Advanced Placement Environmental Science classes
- Activities at KidZu Museum and Life and Sciences Museum
- Short educational Youtube video.