Difference between revisions of "Team:Lethbridge HS/Experiments"

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<h1>Experiments</h1>
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<p>Describe the research, experiments, and protocols you used in your iGEM project. These should be detailed enough for another team to repeat your experiments.</p>
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Please remember to put all characterization and measurement data for your parts on the corresponding Registry part pages.
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<h3>What should this page contain?</h3>
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<li> Protocols </li>
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<li> Experiments </li>
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<li> Documentation of the development of your project </li>
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<li><a href="https://2014.igem.org/Team:Colombia/Protocols">2014 Colombia </a></li>
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<li><a href="https://2014.igem.org/Team:Imperial/Protocols">2014 Imperial </a></li>
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<li><a href="https://2014.igem.org/Team:Caltech/Project/Experiments">2014 Caltech </a></li>
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      <div style="font-family: 'Montserrat' , serif;font-size: 10vw;text-align:center;margin-top:50px;"><center>EXPERIMENTS</center></div>
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<h1  style="font-size: 3vw; font-family:Montserrat;"class="w100" ><b>PROTOCOLS</b></h1>
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    <th><a href="https://static.igem.org/mediawiki/2018/e/e9/T--Lethbridge_HS--agarose.pdf">Agarose Gel Extraction</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/5/5b/T--Lethbridge_HS--colony.pdf">Colony PCR</a></td>
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    <th><a href="https://static.igem.org/mediawiki/2018/9/9c/T--Lethbridge_HS--lbmedia.pdf">LB Media</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/8/89/T--Lethbridge_HS--dna.pdf">DNA Purification from Enzymatic Reactions</a></td>
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    <th><a href="https://static.igem.org/mediawiki/2018/9/90/T--Lethbridge_HS--ligations.pdf">Ligation Reactions</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/6/63/T--Lethbridge_HS--ligationtoinsert.pdf">Ligation to Insert DNA to Vector DNA</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/7/70/T--Lethbridge_HS--pcramplification.pdf">PCR Amplification</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/3/35/T--Lethbridge_HS--pcrproduct.pdf">PCR Product Purification</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/2/29/T--Lethbridge_HS--phenol.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/2/21/T--Lethbridge_HS--phusion.pdf">Phusion PCR Protocol</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/a/a8/T--Lethbridge_HS--ppjet.pdf">pJET Cloning</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/1/1b/T--Lethbridge_HS--plasmid.pdf">Plasmid Miniprep</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/d/d2/T--Lethbridge_HS--preperation.pdf">Preparation of Chemically Competent Cells</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/8/8c/T--Lethbridge_HS--purification.pdf">Protein Overexpression</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/b/b0/T--Lethbridge_HS--restriciton.pdf">Restriction Enzyme Digest and Dephosphorylation</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/3/3e/T--Lethbridge_HS--transformation.pdf">Transformation</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/e/e2/T--Lethbridge_HS--histagged.pdf">Batch purification of his-tagged E.coli proteins with Ni-Sepharose </a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/b/b7/T--Lethbridge_HS--copper.pdf">Copper binding Assay for CutA</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/a/a8/T--Lethbridge_HS--ohGWE.pdf">Phage Infection of E. coli</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/a/ae/T--Lethbridge_HS--SDS.pdf">SDS PAGE of proteins</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/2/24/T--Lethbridge_HS--TRUEE.pdf">Purification of his-tagged TruB</a></th>
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    <th><a href="https://static.igem.org/mediawiki/2018/8/85/T--Lethbridge_HS--ecoli.pdf">Expression of proteins in
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E. coli cells</a></th>
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<p><h1></h1> <b></b>
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Latest revision as of 03:19, 18 October 2018