Difference between revisions of "Team:Lethbridge HS/Experiments"

 
(22 intermediate revisions by 2 users not shown)
Line 69: Line 69:
  
 
table {
 
table {
     font-family: arial, sans-serif;
+
     font-family:'open sans', sans-serif; font-size: 18px;
 
     border-collapse: collapse;
 
     border-collapse: collapse;
 
     width: 100%;
 
     width: 100%;
Line 103: Line 103:
  
 
<div id="allContent">
 
<div id="allContent">
 
+
<br>
<h1> PROTOCOLS</h1>  
+
<h1 style="font-size: 3vw; font-family:Montserrat;"class="w100" ><b>PROTOCOLS</b></h1>
  
 
<table>
 
<table>
 
   <tr>
 
   <tr>
     <th></th>
+
     <th><a href="https://static.igem.org/mediawiki/2018/e/e9/T--Lethbridge_HS--agarose.pdf">Agarose Gel Extraction</a></th>
     <th></th>
+
     <th><a href="https://static.igem.org/mediawiki/2018/5/5b/T--Lethbridge_HS--colony.pdf">Colony PCR</a></td>
  </tr>
+
</tr>
 
    
 
    
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/9/9c/T--Lethbridge_HS--lbmedia.pdf">LB Media</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/8/89/T--Lethbridge_HS--dna.pdf">DNA Purification from Enzymatic Reactions</a></td>
 +
</tr>   
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/9/90/T--Lethbridge_HS--ligations.pdf">Ligation Reactions</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/6/63/T--Lethbridge_HS--ligationtoinsert.pdf">Ligation to Insert DNA to Vector DNA</a></th>
 +
 
 +
  </tr>
 
   <tr>
 
   <tr>
     <td></td>
+
     <th><a href="https://static.igem.org/mediawiki/2018/7/70/T--Lethbridge_HS--pcramplification.pdf">PCR Amplification</a></th>
    <td></td>
+
     <th><a href="https://static.igem.org/mediawiki/2018/3/35/T--Lethbridge_HS--pcrproduct.pdf">PCR Product Purification</a></th>
      
+
  <tr>
+
    <td></td>
+
    <td></td>
+
 
    
 
    
 
   </tr>
 
   </tr>
 
   <tr>
 
   <tr>
     <td></td>
+
     <th><a href="https://static.igem.org/mediawiki/2018/2/29/T--Lethbridge_HS--phenol.pdf">Phenol/Chloroform Extraction and Ethanol Precipitation</a></th>
     <td></td>
+
     <th><a href="https://static.igem.org/mediawiki/2018/2/21/T--Lethbridge_HS--phusion.pdf">Phusion PCR Protocol</a></th>
 
    
 
    
 
   </tr>
 
   </tr>
    
+
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/a/a8/T--Lethbridge_HS--ppjet.pdf">pJET Cloning</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/1/1b/T--Lethbridge_HS--plasmid.pdf">Plasmid Miniprep</a></th>
 +
 
 +
   </tr>
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/d/d2/T--Lethbridge_HS--preperation.pdf">Preparation of Chemically Competent Cells</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/8/8c/T--Lethbridge_HS--purification.pdf">Protein Overexpression</a></th>
 +
 
 +
  </tr>
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/b/b0/T--Lethbridge_HS--restriciton.pdf">Restriction Enzyme Digest and Dephosphorylation</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/3/3e/T--Lethbridge_HS--transformation.pdf">Transformation</a></th>
 +
 
 +
  </tr>
 +
 
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/e/e2/T--Lethbridge_HS--histagged.pdf">Batch purification of his-tagged E.coli proteins with Ni-Sepharose </a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/b/b7/T--Lethbridge_HS--copper.pdf">Copper binding Assay for CutA</a></th>
 +
 
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/a/a8/T--Lethbridge_HS--ohGWE.pdf">Phage Infection of E. coli</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/a/ae/T--Lethbridge_HS--SDS.pdf">SDS PAGE of proteins</a></th>
 +
 
 +
  </tr>
 +
 
 +
<tr>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/2/24/T--Lethbridge_HS--TRUEE.pdf">Purification of his-tagged TruB</a></th>
 +
    <th><a href="https://static.igem.org/mediawiki/2018/8/85/T--Lethbridge_HS--ecoli.pdf">Expression of proteins in
 +
E. coli cells</a></th>
 +
 
 +
  </tr>
 +
  </tr>
 
</table>
 
</table>
  

Latest revision as of 03:19, 18 October 2018