Difference between revisions of "Team:NEU China A/Safety"

 
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                 Just shown in the project design chassis section, we chose a safe chassis of E. coli Nissle 1917 and
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                 Just shown in the project design chassis section, we chose a safe chassis of <i>E. coli Nissle</i> 1917 and
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                 hope to use the genome-modified strain of Nissle SYNB1618 in the future. This strain lacks colonization
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                 hope to use the genome-modified strain of <i>Nissle SYNB1618</i> in the future. This strain lacks colonization
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                 ability and will be emptied within 48 hours. Experimentally, we only used the common DH5α and BL21
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                 ability and will be emptied within 48 hours. Experimentally, we only used the common <i>DH5α</i> and <i>BL21</i>
 
                 Escherichia coli strain to test our system.
 
                 Escherichia coli strain to test our system.
 
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                 Meanwhile, all of our experiments are carried out cautiously, and we don't arbitrarily manipulate model
 
                 Meanwhile, all of our experiments are carried out cautiously, and we don't arbitrarily manipulate model
 
                 until we are sure that our parts will perform the correct function. For example, we would first use GFP
 
                 until we are sure that our parts will perform the correct function. For example, we would first use GFP
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                 and blue chromoprotein (amilCP) rather than a real-world anti-inflammation or anti-tumor molecular to
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                 and blue chromoprotein (amilCP) rather than a real-world anti-inflammatory or anti-tumor molecular to
 
                 establish the model, also we would first use fluorescent proteins indicating our responses/outputs
 
                 establish the model, also we would first use fluorescent proteins indicating our responses/outputs
 
                 rather than IL-10 or myrosinase.
 
                 rather than IL-10 or myrosinase.
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                 There are a lot of fears in the general public's mind when it comes to genetics and genetically
 
                 There are a lot of fears in the general public's mind when it comes to genetics and genetically
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                 modified organisms. One problem that arises with most of these systems is not tackling the problem of
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                 modified organisms. One problem that arises with most of these systems are not tackling the problem of
 
                 biorthogonal DNA escaping into the environment after cell death. In view of these security issues, we
 
                 biorthogonal DNA escaping into the environment after cell death. In view of these security issues, we
 
                 added a kill switch device to our project. We use cold shock expression of the toxic protein mazF to
 
                 added a kill switch device to our project. We use cold shock expression of the toxic protein mazF to
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                 All the Labs we used contained the correct collective protective equipment. Before we carried out our
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                 All the Labs we used contain the correct collective protective equipment. Before we carried out our
 
                 experiments, all of our team members had received lab safety training, including standard experimental
 
                 experiments, all of our team members had received lab safety training, including standard experimental
 
                 protocols, proper disposal of biological and chemical waste and so on. Each of us, at the moment we got
 
                 protocols, proper disposal of biological and chemical waste and so on. Each of us, at the moment we got
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Latest revision as of 03:21, 18 October 2018

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Safety

Safety


Safe Project Design


1.Choosing a non-pathogenic chassis

Just shown in the project design chassis section, we chose a safe chassis of E. coli Nissle 1917 and hope to use the genome-modified strain of Nissle SYNB1618 in the future. This strain lacks colonization ability and will be emptied within 48 hours. Experimentally, we only used the common DH5α and BL21 Escherichia coli strain to test our system.



2.Choosing parts that will be environmentally friendly

The interleukin-10 which we choose to produce is a natural immunosuppressive agent that is secreted under controlled conditions, therefore does not cause harm. IL-10 has been used by the past iGEM teams. For myrosinase, it is a natural component in cruciferous plants. It converts glucosinolates into sulforaphane to exert anti-cancer and inflammation-inhibiting effects. Similarly, people will be exposed to sulforaphane when eating vegetables such as broccoli and kale.
Meanwhile, all of our experiments are carried out cautiously, and we don't arbitrarily manipulate model until we are sure that our parts will perform the correct function. For example, we would first use GFP and blue chromoprotein (amilCP) rather than a real-world anti-inflammatory or anti-tumor molecular to establish the model, also we would first use fluorescent proteins indicating our responses/outputs rather than IL-10 or myrosinase.



3.Kill switch

There are a lot of fears in the general public's mind when it comes to genetics and genetically modified organisms. One problem that arises with most of these systems are not tackling the problem of biorthogonal DNA escaping into the environment after cell death. In view of these security issues, we added a kill switch device to our project. We use cold shock expression of the toxic protein mazF to prevent the harm caused by potentially leaking engineering bacteria.



Safe Lab Work


All the Labs we used contain the correct collective protective equipment. Before we carried out our experiments, all of our team members had received lab safety training, including standard experimental protocols, proper disposal of biological and chemical waste and so on. Each of us, at the moment we got into the Lab, attended to the use of the right individual safety equipment and clothing. Furthermore, in order to ensure complete protection, one has to pay attention to personal hygiene, hand washing is required after each completed job and before leaving the lab.