Difference between revisions of "Team:Cornell/Parts"

 
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     <title>Team:Cornell/Parts - 2018.igem.org</title>
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     <title>Team:Cornell - 2018.igem.org</title>
 
     <!-- CSS -->
 
     <!-- CSS -->
 
     <link rel="stylesheet" type="text/css" href="https://2018.igem.org/Team:Cornell/styles?action=raw&ctype=text/css">
 
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        <!------------------------ NAV BAR START ------------------------>
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<!------------------------ NAV BAR START ------------------------>
 
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                         <a href="home"><img src="https://static.igem.org/mediawiki/2018/6/63/T--Cornell--OscillateLogo.jpg" alt="Oscillate"></a>
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                         <a href="https://2018.igem.org/Team:Cornell"><img src="https://static.igem.org/mediawiki/2018/a/af/T--Cornell--OscillateLogo.png" alt="Oscillate"></a>
 
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                     <div class="nav-bar-main-menu">
 
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                                <a href="https://2018.igem.org/Team:Cornell/Team">BIOS</a>
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                                <a href="https://2018.igem.org/Team:Cornell/Sponsors">SPONSORS</a>
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                            <a href="https://2018.igem.org/Team:Cornell/Sponsors">SPONSORS</a>
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                                <a href="https://2018.igem.org/Team:Cornell/Attributions">ATTRIBUTIONS</a>
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                            <a href="https://2018.igem.org/Team:Cornell/Attributions">ATTRIBUTIONS</a>
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                            </div>
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                                <a href="#">PUBLIC ENGAGEMENT</a>
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                            </div>
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                            <a href="https://2018.igem.org/Team:Cornell/Public_Engagement">PUBLIC ENGAGEMENT</a>
 
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                                <a href="https://2018.igem.org/Team:Cornell/Entrepreneurship">ENTREPRENEURSHIP</a>
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                            </div>
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                            <a href="https://2018.igem.org/Team:Cornell/Entrepreneurship">ENTREPRENEURSHIP</a>
 
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                            <a href="https://2018.igem.org/Team:Cornell/Model">MODELING</a>
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                                <a href="https://2018.igem.org/Team:Cornell/Design">DESIGN</a>
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                            <a href="https://2018.igem.org/Team:Cornell/Testing">TESTING</a>
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                            </div>
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                            <button class="dropbtn active-page">TOOLKIT</button>
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                        <button class="dropbtn active-page">TOOLKIT</button>
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                                <ul>
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                        <div class="dropdown-content toolkit-dropdown-content">
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                                    <div class="nav-first-col">
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                            <ul>
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                                        <li class = "wet-lab-list-title"><b>WET LAB</b></li>
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                                <div class="nav-first-col">
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                                        <li><a href="https://2018.igem.org/Team:Cornell/Foundations">FOUNDATIONS</a></li>
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                                    <li class = "wet-lab-list-title"><b>WET LAB</b></li>
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                                        <li><a href="https://2018.igem.org/Team:Cornell/Demonstrate">DEMONSTRATE</a></li>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/Foundations">FOUNDATIONS</a></li>
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                                        <li><a href="https://2018.igem.org/Team:Cornell/InterLab">INTERLAB</a></li>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/Demonstrate">DEMONSTRATE</a></li>
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                                        <li><a href="hhttps://2018.igem.org/Team:Cornell/Parts">PARTS</a></li>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/InterLab">INTERLAB</a></li>
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                                        </li>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/Parts">PARTS</a></li>
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                                     </div>
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                                     <li><a href="https://2018.igem.org/Team:Cornell/Basic_Part">BASIC PARTS</a></li>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/Composite_Part">COMPOSITE PARTS</a></li>
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                                    <div class="nav-second-col">
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                                    </li>
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                                        <li class = "doc-list-title"><b>DOCUMENTATION</b></li>
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                                </div>
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                                        <li><a href="https://2018.igem.org/Team:Cornell/Notebook">NOTEBOOK</a></li>
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                                 <div class="nav-second-col">
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                                        <li><a href="https://2018.igem.org/Team:Cornell/Safety">SAFETY</a></li>
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                                    <li class = "doc-list-title"><b>DOCUMENTATION</b></li>
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                                        </li>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/Notebook">NOTEBOOK</a></li>
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                                    </div>
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                                    <li><a href="https://2018.igem.org/Team:Cornell/Safety">SAFETY</a></li>
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                                 </ul>
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                                    </li>
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                            </div>
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                            </ul>
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                        <div class="dropdown">
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                            <button class="dropbtn" onclick="location.href='https://2018.igem.org/Team:Cornell/Description '">ABOUT</button>
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                        </div>
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−
                        <div class="dropdown">
+
−
                            <button class="dropbtn" onclick="location.href='https://2018.igem.org/Team:Cornell'">HOME</button>
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                         </div>
 
                         </div>
 
                     </div>
 
                     </div>
 +
                    <div class="dropdown">
 +
                        <button class="dropbtn" onclick="location.href='https://2018.igem.org/Team:Cornell/About'">ABOUT</button>
 +
                    </div>
 +
                    <div class="dropdown">
 +
                        <button class="dropbtn" onclick="location.href='https://2018.igem.org/Team:Cornell'">HOME</button>
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                 </div>
 
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             </div>
 
             </div>
 
         </nav>
 
         </nav>
 
         <!------------------------ NAV BAR END ------------------------>
 
         <!------------------------ NAV BAR END ------------------------>
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        <!------------------------ PARTS PAGE BANNER START ------------------------>
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<!------------------------ WET LAB PAGE BANNER START ------------------------>
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         <header class="standard-page-banner parts-page-banner">           
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         <header class="parts-page-banner standard-page-banner">           
 
             <svg viewBox="0 0 100 100" width=100% height=100%>
 
             <svg viewBox="0 0 100 100" width=100% height=100%>
 
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         </header>
 
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         <!------------------------ PARTS PAGE BANNER END ------------------------>
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            <!------------------------ WET LAB PAGE CONTENT START ------------------------>
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            <div class="parts-page-content-wrapper">
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<div class="parts-page-content-section-last">
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<table class="tg" bordercolor = "#000000">
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  <tr>
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<th class="tg-0lax">BioBrick Number</th>
 +
<th class="tg-0lax">Name</th>
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<th class="tg-0lax">Type</th>
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<th class="tg-0lax">Description</th>
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<th class="tg-0lax">Designer</th>
 +
  </tr>
 +
  <tr>
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<td class="tg-0lax">BBa_K2561000</td>
 +
<td class="tg-0lax">RNA Thermometer</td>
 +
<td class="tg-0lax">Basic</td>
 +
<td class="tg-0lax">An RNA thermometer active around 37°C</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561001</td>
 +
<td class="tg-0lax">σ<sup>F</sup></td>
 +
<td class="tg-0lax">Basic</td>
 +
<td class="tg-0lax"><i>B. subtilis</i> sigma factor for orthogonal expression in <i>E. coli</i></td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561002</td>
 +
<td class="tg-0lax">P<sub>F2</sub></td>
 +
<td class="tg-0lax">Basic</td>
 +
<td class="tg-0lax">σ<sup>F</sup> promoter</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
                                          <tr>
 +
<td class="tg-0lax">BBa_K2561008</td>
 +
<td class="tg-0lax">hrpR</td>
 +
<td class="tg-0lax">Basic</td>
 +
<td class="tg-0lax">Enhancer binding protein as hrpRS dimer. Part of an AND logic gate</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
                                          <tr>
 +
<td class="tg-0lax">BBa_K2561009</td>
 +
<td class="tg-0lax">hrpS</td>
 +
<td class="tg-0lax">Basic</td>
 +
<td class="tg-0lax">Enhancer binding protein as hrpRS dimer. Part of an AND logic gate</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
                                          <tr>
 +
<td class="tg-0lax">BBa_K2561010</td>
 +
<td class="tg-0lax">hrpL promoter</td>
 +
<td class="tg-0lax">Basic</td>
 +
<td class="tg-0lax">𝛔<sup>54</sup>-recognized promoter whose activity is mediated by hrpRS EBP</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561003</td>
 +
<td class="tg-0lax">Low Pass (GFP)</td>
 +
<td class="tg-0lax">Composite</td>
 +
<td class="tg-0lax">Low pass filter with sfGFP</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561004</td>
 +
<td class="tg-0lax">Low Pass (AND)</td>
 +
<td class="tg-0lax">Composite</td>
 +
<td class="tg-0lax">Low pass filter with hrpS component of AND gate</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561005</td>
 +
<td class="tg-0lax">High Pass (GFP)</td>
 +
<td class="tg-0lax">Composite</td>
 +
<td class="tg-0lax">High pass filter with sfGFP</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561006</td>
 +
<td class="tg-0lax">High Pass (AND)</td>
 +
<td class="tg-0lax">Composite</td>
 +
<td class="tg-0lax">High pass filter with hrpR component of AND gate</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
  <tr>
 +
<td class="tg-0lax">BBa_K2561007</td>
 +
<td class="tg-0lax">AND Reporter</td>
 +
<td class="tg-0lax">Composite</td>
 +
<td class="tg-0lax">P<sub>hrpL</sub> promoter with sfGFP</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
                                          <tr>
 +
<td class="tg-0lax">BBa_K2561011</td>
 +
<td class="tg-0lax">High Pass (AND) + Reporter</td>
 +
<td class="tg-0lax">Composite</td>
 +
<td class="tg-0lax">High pass filter with hrpR component of AND gate (BBa_K2561006)and reporter construct (BBa_K2561007)</td>
 +
<td class="tg-0lax">Amith Punyala</td>
 +
  </tr>
 +
</table>
 +
</div>
 +
            </div>
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                <p class="parts-body-text">We are a team composed entirely of undergraduates and we pride ourselves on our independence. The concept of a biological band-pass filter based on frequency was brainstormed and implemented by our team. Our wet lab team designed and cloned all sequences into pSB1C3, and transformed them for testing in DH5-α E. Coli. They also designed all experiments and worked to analyze all the data we collected. The product development group worked to create the mathematical model for the system based on Hill Kinetics. The sub-team worked on both the deterministic and stochastic model. The policy and practices team spearheaded our outreach efforts and led initiatives to contact and work with synthetic biology researches on our campus and beyond. To understand how our project might integrate into the field, they conducted interviews to find out about how developing technologies enter a new field.</p>
 
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Latest revision as of 03:25, 18 October 2018

Team:Cornell - 2018.igem.org

Parts
BioBrick Number Name Type Description Designer
BBa_K2561000 RNA Thermometer Basic An RNA thermometer active around 37°C Amith Punyala
BBa_K2561001 σF Basic B. subtilis sigma factor for orthogonal expression in E. coli Amith Punyala
BBa_K2561002 PF2 Basic σF promoter Amith Punyala
BBa_K2561008 hrpR Basic Enhancer binding protein as hrpRS dimer. Part of an AND logic gate Amith Punyala
BBa_K2561009 hrpS Basic Enhancer binding protein as hrpRS dimer. Part of an AND logic gate Amith Punyala
BBa_K2561010 hrpL promoter Basic 𝛔54-recognized promoter whose activity is mediated by hrpRS EBP Amith Punyala
BBa_K2561003 Low Pass (GFP) Composite Low pass filter with sfGFP Amith Punyala
BBa_K2561004 Low Pass (AND) Composite Low pass filter with hrpS component of AND gate Amith Punyala
BBa_K2561005 High Pass (GFP) Composite High pass filter with sfGFP Amith Punyala
BBa_K2561006 High Pass (AND) Composite High pass filter with hrpR component of AND gate Amith Punyala
BBa_K2561007 AND Reporter Composite PhrpL promoter with sfGFP Amith Punyala
BBa_K2561011 High Pass (AND) + Reporter Composite High pass filter with hrpR component of AND gate (BBa_K2561006)and reporter construct (BBa_K2561007) Amith Punyala