Difference between revisions of "Team:TecCEM/Safety"

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<h1> Safety </h1>
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<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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<div id="gif-title" class="text-safety">
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    <img src="https://static.igem.org/mediawiki/2018/8/81/T--TecCEM--Cells--Safety.gif" alt="Cell Gif">
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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    <h1>Safety</h1>
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<div class="container my-4">
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    <div class="row">
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        <div class="col">
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            <p class="lead">Lab work and project safety have to be carefully measured and taken precariously by
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                everyone
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                involved
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                while conducting experiments. Most precautions have to be carried out taking note of all the
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                safety
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                protocols concerned. </p>
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            <p class="semi-lead">Since all our parts were designed to be specific and effective toward cell
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                lines, the only
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                precautions
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                taken were level 1 for safety measures. All the strains used come from E. coli strain and were
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                inactivated as the safety protocols indicate.</p>
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            <div>
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                <figure class="figure text-center w-100">
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                    <img style="max-height: 70vh;" src="https://static.igem.org/mediawiki/2018/b/b9/T--TecCEM--Safety3.jpg"
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                        class="figure-img img-fluid rounded" alt="IMP-1">
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                    <figcaption class="figure-caption"><strong>Figure 1. Safety iGEM TecCEM example</strong></figcaption>
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                </figure>
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            </div>
  
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<div class="column two_thirds_size">
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            <h3 id="general">General lab safety for ourselves</h3>
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<h3>Safe Project Design</h3>
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            <figure class="figure text-center w-50 float-left">
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                <img style="max-height: 40vh;" src="https://static.igem.org/mediawiki/2018/c/c4/T--TecCEM--SafetyRod.jpg"
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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                    class="figure-img img-fluid rounded" alt="IMP-1">
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                <figcaption class="figure-caption"><strong>Figure 2. Member using lab coat.</strong></figcaption>
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<ul>
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            </figure>
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<li>Choosing a non-pathogenic chassis</li>
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            <p>For our own safety, prior to beginning any lab work, a brief introduction to all the safety
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<li>Choosing parts that will not harm humans / animals / plants</li>
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                protocols
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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                was given. A quick course of lab equipment manipulation and lab responsibility was taken by all
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<li>Including an "induced lethality" or "kill-switch" device</li>
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                the
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</ul>
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                team members, helping to give a brief introduction to working in a lab for those that hadn’t
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                worked
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                before in one and as a reinforcement for the others.</p>
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            <p>As a level 1 in biosafety, the right safety protocols were learned and revised. This was done to
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                assure
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                that the right decontamination and disposal of materials were being carried out, the right
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                protective
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                equipment was being used and all the standard lab rules were being followed as indicated by our
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                laboratory technicians.</p>
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            <p>Our laboratory has the standard safety equipment such as emergency showers, eyewash, fire
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                detectors,
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                fire extinguisher, security sheets, waste disposal, autoclaving, laminar flux bells, and glove
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                dispenser. We always made sure to wear the right lab equipment such as lab coats, glasses,
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                closed
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                shoes, gloves, the right clothing, and tied hair.</p>
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            <h3 id="protecting">Protecting the environment protocols</h3>
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            <p>We have a responsibility to take care of the environment. For our experiments, the use of
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                antibiotics
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                for selection had to be carefully disposed of with the right protocols.</p>
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            <p>Some of the antibiotics used were: kanamycin and chloramphenicol. The safety sheet for
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                chloramphenicol
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                these compound can cause respiratory problems, it’s hazardous for ingestion and causes germ
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                cell
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                mutagenicity, reproductive toxicity and aspiration hazard all of level 1 category.</p>
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        </div>
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    </div>
 
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<div class="column third_size">
 
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<h3>Safe Lab Work</h3>
 
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
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<h3>Safe Shipment</h3>
 
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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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</div>
 
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</html>
 
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{{TecCEM/Footer}}

Latest revision as of 03:36, 18 October 2018

Cell Gif

Safety

Lab work and project safety have to be carefully measured and taken precariously by everyone involved while conducting experiments. Most precautions have to be carried out taking note of all the safety protocols concerned.

Since all our parts were designed to be specific and effective toward cell lines, the only precautions taken were level 1 for safety measures. All the strains used come from E. coli strain and were inactivated as the safety protocols indicate.

IMP-1
Figure 1. Safety iGEM TecCEM example

General lab safety for ourselves

IMP-1
Figure 2. Member using lab coat.

For our own safety, prior to beginning any lab work, a brief introduction to all the safety protocols was given. A quick course of lab equipment manipulation and lab responsibility was taken by all the team members, helping to give a brief introduction to working in a lab for those that hadn’t worked before in one and as a reinforcement for the others.

As a level 1 in biosafety, the right safety protocols were learned and revised. This was done to assure that the right decontamination and disposal of materials were being carried out, the right protective equipment was being used and all the standard lab rules were being followed as indicated by our laboratory technicians.

Our laboratory has the standard safety equipment such as emergency showers, eyewash, fire detectors, fire extinguisher, security sheets, waste disposal, autoclaving, laminar flux bells, and glove dispenser. We always made sure to wear the right lab equipment such as lab coats, glasses, closed shoes, gloves, the right clothing, and tied hair.

Protecting the environment protocols

We have a responsibility to take care of the environment. For our experiments, the use of antibiotics for selection had to be carefully disposed of with the right protocols.

Some of the antibiotics used were: kanamycin and chloramphenicol. The safety sheet for chloramphenicol these compound can cause respiratory problems, it’s hazardous for ingestion and causes germ cell mutagenicity, reproductive toxicity and aspiration hazard all of level 1 category.