Difference between revisions of "Team:HZAU-China/Human Practices"

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{{HZAU-China}}
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{{HZAU-China/Bootstrap}}
 
<html>
 
<html>
  
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<div class="column full_size judges-will-not-evaluate">
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<head>
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<h3>★  ALERT! </h3>
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    <meta charset="UTF-8">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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    <meta name="viewport" content="width=device-width, initial-scale=1.0, minimum-scale=1.0, maximum-scale=1.0, user-scalable=no">
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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    <link href="bootstrap.min.css" rel="stylesheet">
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</div>
+
    <!-- 导航栏CSS -->
 +
    <style type="text/css">
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        #sideMenu,
 +
        #top_title,
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 +
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 +
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<h1>Human Practices</h1>
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<p>
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            height: 16px;
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At iGEM we believe societal considerations should be upfront and integrated throughout the design and execution of synthetic biology projects. “Human Practices” refers to iGEM teams’ efforts to actively consider how the world affects their work and the work affects the world. Through your Human Practices activities, your team should demonstrate how you have thought carefully and creatively about whether your project is responsible and good for the world. We invite you to explore issues relating (but not limited) to the ethics, safety, security, and sustainability of your project, and to show how this exploration feeds back into your project purpose, design and execution.
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        }
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</p>
+
  
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 +
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 +
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<p>For more information, please see the <a href="https://2018.igem.org/Human_Practices">Human Practices Hub</a>. There you will find:</p>
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        .daohang {
−
+
            background-color: #323643;
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<ul>
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            position: relative;
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<li> an <a href="https://2018.igem.org/Human_Practices/Introduction">introduction</a> to Human Practices at iGEM </li>
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            color: #ffffff;
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<li>tips on <a href="https://2018.igem.org/Human_Practices/How_to_Succeed">how to succeed</a> including explanations of judging criteria and advice about how to conduct and document your Human Practices work</li>
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            height: 64px;
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<li>descriptions of <a href="https://2018.igem.org/Human_Practices/Examples">exemplary work</a> to inspire you</li>
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            top: -2px;
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<li>links to helpful <a href="https://2018.igem.org/Human_Practices/Resources">resources</a></li>
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        }
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<li>And more! </li>
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−
</ul>
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−
+
  
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<p>On this page, your team should document all of your Human Practices work and activities. You should write about the Human Practices topics you considered in your project, document any activities you conducted to explore these topics (such as engaging with experts and stakeholders), describe why you took a particular approach (including referencing any work you built upon), and explain if and how you integrated takeaways from your Human Practices work back into your project purpose, design and/or execution. </p>
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        .daohang .caidan {
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            position: absolute;
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<p>If your team has gone above and beyond in work related to safety, then you should document this work on your Safety wiki page and provide a description and link on this page. If your team has developed education and public engagement efforts that go beyond a focus on your particular project, and for which would like to nominate your team for the Best Education and Public Engagement Special Prize, you should document this work on your Education and Education wiki page and provide a description and link here. </p>
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<p>The iGEM judges will review this page to assess whether you have met the Silver and/or Gold medal requirements based on the Integrated Human Practices criteria listed below. If you nominate your team for the <a href="https://2018.igem.org/Judging/Awards">Best Integrated Human Practices Special Prize</a> by filling out the corresponding field in the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>, the judges will also review this page to consider your team for that prize.
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 +
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 +
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 +
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<div class="column full_size">
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        .daohang .xsjPic {
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            display: inline-block;
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<h3>Silver Medal Criterion #3</h3>
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            width: 10px;
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<p>Convince the judges you have thought carefully and creatively about whether your work is responsible and good for the world. Document how you have investigated these issues and engaged with your relevant communities, why you chose this approach, and what you have learned. Please note that surveys will not fulfill this criteria unless you follow scientifically valid methods. </p>
+
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 +
            vertical-align: middle;
 +
            background-size: 100% 100%;
 +
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 +
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 +
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 +
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 +
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 +
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 +
            vertical-align: middle;
 +
            background-size: 100% 100%;
 +
            background-image: url("https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg")
 +
        }
  
−
<h3>Gold Medal Criterion #1</h3>
+
        .daohang a:hover span {
−
<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the purpose, design and/or execution of your project. Document how your project has changed based upon your human practices work.
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−
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−
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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−
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+
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 +
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 +
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−
<div class="column full_size">
+
        .daohang .caidan>li:hover .item {}
−
<h3>Best Integrated Human Practices Special Prize</h3>
+
  
−
<p>To compete for the Best Integrated Human Practices prize, please describe your work on this page and also fill out the description on the judging form. </p>
 
  
−
<p>How does your project affect society and how does society influence the direction of your project? How might ethical considerations and stakeholder input guide your project purpose and design and the experiments you conduct in the lab? How does this feedback enter into the process of your work all through the iGEM competition? Document a thoughtful and creative approach to exploring these questions and how your project evolved in the process to compete for this award!</p>
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        .daohang .shortName .item:before {
−
<p>You must also delete the message box on the top of this page to be eligible for this prize.</p>
+
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 +
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            bottom: 5px;
 +
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 +
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−
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            border-right: 3px solid #323643;
 +
        }
 +
 
 +
        .laoshi img {
 +
            width: 100%;
 +
            height: 50vh;
 +
            border-top: 3px solid #323643;
 +
            border-left: 3px solid #323643;
 +
            border-right: 3px solid #323643;
 +
        }
 +
 
 +
        .duiyuan .xingming {
 +
            width: 100%;
 +
            height: 50px;
 +
            line-height: 50px;
 +
            background-color: #323643;
 +
            border-left: 3px solid #323643;
 +
            border-right: 3px solid #323643;
 +
            font-size: 16px;
 +
            color: white;
 +
            box-shadow: 0 1px 3px #CCCCCC;
 +
            font-family: Cambria, Cochin, Georgia, Times, 'Times New Roman', serif;
 +
            font-size: 24px;
 +
            font-weight: bold;
 +
            text-align: center;
 +
        }
 +
 
 +
        .laoshi .xingming {
 +
            width: 100%;
 +
            height: 50px;
 +
            line-height: 50px;
 +
            background-color: #323643;
 +
            border-left: 3px solid #323643;
 +
            border-right: 3px solid #323643;
 +
            font-size: 16px;
 +
            color: white;
 +
            box-shadow: 0 1px 3px #CCCCCC;
 +
            font-family: Cambria, Cochin, Georgia, Times, 'Times New Roman', serif;
 +
            font-size: 24px;
 +
            font-weight: bold;
 +
            text-align: center;
 +
        }
 +
 
 +
        .duiyuan .jieshao {
 +
            width: 100%;
 +
            height: 200px;
 +
            background-color: #f6eee0;
 +
            border-bottom: 3px solid #323643;
 +
            border-left: 3px solid #323643;
 +
            border-right: 3px solid #323643;
 +
            /* border-bottom-left-radius: 5px;
 +
            border-bottom-right-radius: 5px; */
 +
        }
 +
 
 +
        .duiyuan .jieshao .saohua {
 +
            padding: 10px 4px 0px 4px !important;
 +
            color: black;
 +
            font-family: Arial;
 +
            font-style: italic;
 +
            font-size: 15px !important;
 +
            list-style-type: none;
 +
            text-align: justify;
 +
            line-height: 18px;
 +
        }
 +
    </style>
 +
    <!-- 滚动菜单栏CSS -->
 +
    <style>
 +
        #float01 {}
 +
 
 +
        #float02 {}
 +
 
 +
        div.floatCtro {
 +
            width: 100%;
 +
            /* height: auto; */
 +
            margin: 0;
 +
            position: relative;
 +
            background: #fff;
 +
 
 +
        }
 +
 
 +
        .daohangyi {
 +
            display: block;
 +
            width: 100%;
 +
            /* height: auto; */
 +
            /* text-align: left !important; */
 +
            color: #ffffff !important;
 +
            font-size: 16px;
 +
            padding:0;
 +
            background-color: #323643;
 +
            border-bottom: 1px solid black;
 +
            padding:0 3%;
 +
            text-decoration: none !important;
 +
        }
 +
 
 +
        div.floatCtro .daohanger {
 +
            width: 100%;
 +
            text-align: left !important;
 +
            height: auto;
 +
            line-height: 25px;
 +
            font-family: Arial;
 +
            font-size: 14px !important;
 +
            color: #676767;
 +
            margin: 0;
 +
            padding:10px 8%;
 +
            cursor: pointer;
 +
            background: #fff;
 +
        }
 +
 
 +
        div.floatCtro a {
 +
            display: inline-block;
 +
            display: none;
 +
            width: 100%;
 +
            height: 40px;
 +
            padding:10px 8%;
 +
            /* margin: 10px 0 0 0; */
 +
            background: #FFF;
 +
            color: #000 !important;
 +
            vertical-align: middle;
 +
            cursor: pointer;
 +
        }
 +
 
 +
        div.floatCtro a span {
 +
            display: block;
 +
            height: auto;
 +
            text-align: left !important;
 +
            line-height: 18px;
 +
            font-family: Arial;
 +
            font-size: 16px;
 +
        }
 +
 
 +
        div.floatCtro a:hover {
 +
            /* background: #76b39d; */
 +
            color: #EA0D04 !important;
 +
            zoom: 1;
 +
        }
 +
 
 +
        /* .cebian a:hover {
 +
            color: #f6eee0 !important;
 +
        } */
 +
 
 +
        div.floatCtro .daohanger:hover {
 +
            /* background: #76b39d; */
 +
            color: #1fa67a;
 +
            text-decoration: underline !important;
 +
        }
 +
 
 +
        div.floatCtro .daohanger.cur {
 +
            /* background: #1fa67a; */
 +
            color: #1fa67a;
 +
        }
 +
 
 +
        .biaoti {
 +
            display: inline-block;
 +
            margin: 8px 0;
 +
            /* width: 50%; */
 +
            /* margin-left: 6%; */
 +
            /* text-align: center; */
 +
            text-decoration: none !important;
 +
        }
 +
 
 +
        .daohangyi img {
 +
            display: inline-block;
 +
            width: 12px;
 +
            height: 12px;
 +
            vertical-align: middle;
 +
            background-size: 100% 100%;
 +
 
 +
        }
 +
        .cebian div:hover img{
 +
            transform: rotateY(180deg);           
 +
            -webkit-transform: rotateY(180deg);           
 +
            -moz-transform: rotateY(180deg);           
 +
            -o-transform: rotateY(180deg);           
 +
            -ms-transform: rotateY(180deg);
 +
            transition: all 0.5s ease-in-out;           
 +
            -webkit-transition: all 0.5s ease-in-out;           
 +
            -moz-transition: all 0.5s ease-in-out;           
 +
            -o-transition: all 0.5s ease-in-out;
 +
        }
 +
        .cebian a:hover img{
 +
            transform: rotate(360deg);           
 +
            -webkit-transform: rotate(360deg);           
 +
            -moz-transform: rotate(360deg);           
 +
            -o-transform: rotate(360deg);           
 +
            -ms-transform: rotate(360deg);
 +
            transition: all 0.5s ease-in-out;           
 +
            -webkit-transition: all 0.5s ease-in-out;           
 +
            -moz-transition: all 0.5s ease-in-out;           
 +
            -o-transition: all 0.5s ease-in-out;
 +
        }
 +
 
 +
        @media screen and (max-width: 1200px) {           
 +
         
 +
            .cebian {
 +
            width: 15%;
 +
            }
 +
 
 +
            .zhengwen {
 +
            margin: 20px 20px 0 0;
 +
 
 +
            .daohangyi {
 +
                font-size: 14px;
 +
            }
 +
            .daohangyi img {
 +
                display: none;
 +
            } 
 +
        }
 +
 
 +
        @media screen and (max-width: 900px) {
 +
 
 +
            .daohangyi img {
 +
                display: none;
 +
            } 
 +
           
 +
            .laoshi img {
 +
                height: 30vh;
 +
            }
 +
        }
 +
    </style>
 +
    <!-- 折叠框CSS -->
 +
    <style>
 +
        .collapseDiv {
 +
          color: #333;
 +
          border-radius: 4px;
 +
          background-color: #F3F3F3;
 +
          border: 1px solid transparent;
 +
          border-color: #ddd;
 +
          box-shadow: 0 1px 1px #F3F3F3;
 +
          margin-top: 5px;
 +
          margin-bottom: 0;
 +
        }
 +
        .collapseDiv label {
 +
          cursor: pointer;
 +
          font-size: 24px  !important;
 +
          /* font-weight: bold; */
 +
          color: black  !important;
 +
          border-color: #F3F3F3  !important;
 +
          padding: 5px 15px 5px 18px  !important;
 +
          background-color: #f5f5f5  !important;
 +
          margin: 0  !important;
 +
          text-align: center !important;
 +
          border-radius: 5px  !important;
 +
        }
 +
 
 +
        #zhedie-toggle1,
 +
        #zhedie-toggle2,
 +
        #zhedie-toggle3,
 +
        #zhedie-toggle4 {
 +
            display: none;
 +
        }
 +
 
 +
        #zhedie1,
 +
        #zhedie2,
 +
        #zhedie3,
 +
        #zhedie4 {
 +
            display: none;
 +
            font-size: 18px;
 +
            padding: 0 50px 0 50px;
 +
            width: 100%;
 +
            margin: 0;
 +
            color: black;
 +
        }
 +
 
 +
        .zhedie{
 +
            font-size: 18px;
 +
            padding: 0 50px 0 50px;
 +
            width: 100%;
 +
            margin: 0;
 +
            color: black;
 +
        }
 +
 
 +
        #zhedie-toggle1:checked+#zhedie1,
 +
        #zhedie-toggle2:checked+#zhedie2,
 +
        #zhedie-toggle3:checked+#zhedie3,
 +
        #zhedie-toggle4:checked+#zhedie4 {
 +
            display: block;
 +
        }
 +
 
 +
       
 +
 
 +
 
 +
        .collapse_body {
 +
          background-color: #fff;
 +
          position: relative;
 +
          height: 0;
 +
          overflow: hidden;
 +
          -webkit-transition-timing-function: ease;
 +
          -o-transition-timing-function: ease;
 +
          transition-timing-function: ease;
 +
          -webkit-transition-duration: .35s;
 +
          -o-transition-duration: .35s;
 +
          transition-duration: .35s;
 +
          -webkit-transition-property: height, visibility;
 +
          -o-transition-property: height, visibility;
 +
          transition-property: height, visibility
 +
        }
 +
        .collapse_content {
 +
          font-size: 20px;
 +
          border-top: 1px solid #ddd;
 +
          background-color: #fff;
 +
          padding: 15px;
 +
        }
 +
    </style>
 +
 
 +
 
 +
</head>
 +
 
 +
<body>
 +
    <!-- 导航栏 -->
 +
    <nav class="daohang">
 +
        <a href="https://2018.igem.org/Team:HZAU-China"><img src="https://static.igem.org/mediawiki/2018/7/75/T--HZAU-China--logo-nav.png"
 +
                class="logo-daohang"></a>
 +
        <ul class="caidan">
 +
            <li class="hiLight shortName">
 +
                <a class="nav_head" href="https://2018.igem.org/Team:HZAU-China">
 +
                    <span>Project</span>
 +
                    <span class="xsjPic"></span>
 +
                </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Description">Description</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Design">Design</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Results">Results</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Demonstrate">Demonstrate</a>
 +
            </li>
 +
            <li class="hiLight shortName">
 +
                <a class="nav_head" href="#">
 +
                    <span>Wetlab</span>
 +
                    <span class="xjtPic"></span>
 +
                </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Experiments">Experiments</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Improve">Improve</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/InterLab">InterLab</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Notebook">Notebook</a>
 +
 
 +
            </li>
 +
            <li class="hiLight shortName">
 +
                <a class="nav_head" href="#">
 +
                    <span>Drylab</span>
 +
                    <span class="xjtPic"></span>
 +
                </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Model">Model</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Software">Software</a>
 +
            </li>
 +
            <li class="hiLight longName">
 +
                <a class="nav_head" href="#">
 +
                    <span>Human&nbsp;Practices</span>
 +
                    <span class="xjtPic"></span>
 +
                </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Human_Practices">Human Practices</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Public Engagement</a>
 +
            </li>
 +
            <li class="hiLight shortName">
 +
                <a class="nav_head" href="#">
 +
                    <span>About Us</span>
 +
                    <span class="xjtPic"></span>
 +
                </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Team">Team</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Attributions">Attributions</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Collaborations">Collaborations</a>
 +
            </li>
 +
            <li class="hiLight shortName">
 +
                <a class="nav_head" href="#">
 +
                    <span>Parts</span>
 +
                    <span class="xjtPic"></span>
 +
                </a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Basic_Part">Basic</a>
 +
                <a class="item" href="https://2018.igem.org/Team:HZAU-China/Composite_Part">Composite</a>
 +
            </li>
 +
        </ul>
 +
        <div class="shade"></div>
 +
    </nav>
 +
    <!-- 内容 -->
 +
    <div class="neirong clearfix">
 +
        <!-- 正文 -->
 +
        <div class="zhengwen">
 +
 
 +
            <div id="float01" class="cur">
 +
                <div class="h1">Overview</div>
 +
                <p>As the Chinese saying goes, “know the enemy and know yourself, and you can fight a hundred battles
 +
                    with no danger of defeat”. In the battle with cancer, we have gained great knowledge about the
 +
                    “enemy”, as well as our own immune system. However, despite the success in curing early and middle
 +
                    stage cancers we have made, the lack of public comprehension is still likely to cause panic. We
 +
                    sensed that our mission is to help the public learn more about cancer and its treatment, as well as
 +
                    the difference that synthetic biology can make in future approach. To accomplish that, we held a
 +
                    live seminar to share our idea and then started to blog newly emerged cancer therapies, basic
 +
                    aspects of synthetic biology and our project. Meanwhile, letting the public know that their voices
 +
                    have been heard is also very important, so we collected their opinions carefully through public
 +
                    survey, designed new circuits for their safety concerns, and invited them for discussion on our
 +
                    team blog. To explore the further application and the potential risk, we also communicated with
 +
                    some cancer experts. We want to be a communicating bridge during this battle where information is
 +
                    accessible to everyone who needs it.</p>
 +
                <div style="width: 70%; margin: 30px auto">
 +
                    <img src="https://static.igem.org/mediawiki/2018/a/ab/T--HZAU-China--hp1.png" width=100% alt="">
 +
                </div>
 +
 
 +
            </div>
 +
            <div id="float02">
 +
                <div class="h1">Silver Human Practice</div>
 +
                <div class="h2"><a href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a></div>
 +
                <p>This year, we have done a lot of work in order to meet the requirement of biosafety. After
 +
                    collecting opinions from experts and the public, we learned that the general concerns are about:
 +
                    How is it safe to apply bacteriotherapy against cancer without causing unexpected damage or
 +
                    infection? We took these concerns seriously and have found some practical solutions.</p>
 +
 
 +
 
 +
                <div class="h2">EXPERT interviews</div>
 +
 
 +
 
 +
                <div style="margin: 20px 0;" class="row">
 +
 
 +
                    <div class="col-xs-12 col-sm-3 col-md-3 col-lg-3">
 +
                        <img style="border:4px solid #E0CCAE; box-shadow: 0 1px 3px #676767; " src="https://static.igem.org/mediawiki/2018/1/16/T--HZAU-China--laoshihe.jpg"
 +
                            width=100% alt="">
 +
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 +
 
 +
 
 +
 
 +
                    <div class="col-xs-12 col-sm-9 col-md-9 col-lg-9">
 +
                        <div class="collapseDiv">
 +
                            <label for="zhedie-toggle1">Prof. Jin He</label>
 +
                            <input type="checkbox" id="zhedie-toggle1" />
 +
                            <div id="zhedie1" class="text-success text-left">
 +
                                <b>College of Life Science and Technology, Huazhong Agricultural University.</b> <br>
 +
                                Professor Jin He has given us a lot of useful suggestions and instructions. As an
 +
                                expert in
 +
                                microbes, he points out that <i>Salmonella</i> is a kind of safe bacterium which is
 +
                                widely
 +
                                used in
 +
                                researches around the world. After knocking out a series of genes, the harm of
 +
                                <i>Salmonella</i>
 +
                                is
 +
                                acceptable. He also points out that <i>Salmonella</i> has very common with <i>E.coli</i>
 +
                                on behalf
 +
                                that
 +
                                both
 +
                                bacteria are in Enterobacteriaceae. This means that we can incubate <i>Salmonella</i>
 +
                                on the
 +
                                same
 +
                                condition and cultivation with <i>E. coli</i>. In addition, <i>Salmonella</i> is very
 +
                                similar to <i>E.
 +
                                    coli</i>
 +
                                in
 +
                                genetics, which means many metabolic reactions happen in <i>E. coli</i> would also
 +
                                happen in
 +
                                <i>Salmonella</i>. Besides, he tells us that there are many intracellular parasites
 +
                                well
 +
                                studied
 +
                                such
 +
                                as Listeria , so we have many choices in the future. In conclusion, his indispensable
 +
                                instructions have made up our minds to engineer on <i>Salmonella</i>.
 +
                                <br><br>
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
                <div style="margin: 20px 0;" class="row">
 +
                    <div class="col-xs-12 col-sm-3 col-md-3 col-lg-3">
 +
                        <img style="border:4px solid #E0CCAE; box-shadow: 0 1px 3px #676767; " src="https://static.igem.org/mediawiki/2018/0/08/T--HZAU-China--laoshijia.jpg"
 +
                            width=100% alt="">
 +
                    </div>
 +
                    <div class="col-xs-12 col-sm-9 col-md-9 col-lg-9">
 +
 
 +
                        <div class="collapseDiv">
 +
                            <label for="zhedie-toggle2">Dr. Qike Jia</label>
 +
                            <input type="checkbox" id="zhedie-toggle2" />
 +
                            <div id="zhedie2" class="text-success text-left">
 +
                                <b>Doctor of Hubei Tumor Hospital, Wuhan, Hubei Province, PRC.</b> <br>
 +
                                Doctor Qike Jia is a young doctor works in Hubei Tumor Hospital and has some experiences
 +
                                in
 +
                                tumor treatment. He is familiar with all popular tumor therapies in China and has given
 +
                                us
 +
                                an
 +
                                overview of tumor treatments. When asked about what the most used therapy at present
 +
                                is,
 +
                                the
 +
                                doctor directly pointed out that it is surgery without hesitate. He thinks that
 +
                                operating
 +
                                on
 +
                                patients is always the first thing to do when a doctor is facing a tumor in medium or
 +
                                late
 +
                                stage. After cutting off tumor issues, radiation and chemical therapies can be carried
 +
                                out
 +
                                for
 +
                                further clearance of tumor cells. When asked about the opinion to our project, he
 +
                                encouraged us
 +
                                with a big smile on his face. He said our project is quite good and expectable,
 +
                                although we
 +
                                may
 +
                                have faced some trouble. Cell cultivation is a really hard work taking a lot of time
 +
                                and
 +
                                spirits. Even if we could complete the project, we still need an enormous amount of
 +
                                efforts
 +
                                to
 +
                                push our therapy to the market. He hoped that we could overcome all the difficulties
 +
                                and
 +
                                gain
 +
                                the final success.
 +
                                <br><br>
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
                <div style="margin: 20px 0;" class="row">
 +
                    <div class="col-xs-12 col-sm-3 col-md-3 col-lg-3">
 +
                        <img style="border:4px solid #E0CCAE; box-shadow: 0 1px 3px #676767; " src="https://static.igem.org/mediawiki/2018/f/f1/T--HZAU-China--laoshiqi.jpg"
 +
                            width=100% alt="">
 +
                    </div>
 +
                    <div class="col-xs-12 col-sm-9 col-md-9 col-lg-9">
 +
 
 +
                        <div class="collapseDiv">
 +
                            <label for="zhedie-toggle3">Dr. Yingchun Qi</label>
 +
                            <input type="checkbox" id="zhedie-toggle3" />
 +
                            <div id="zhedie3" class="text-success text-left">
 +
                                <b>HZAU biological experimental teaching center deputy director
 +
                                </b>
 +
                                Dr. Yingchun Qi is one of the people who have the closest connection with our lab.
 +
                                She
 +
                                is
 +
                                majored in cell culture and genetic transformation. Every year, she will conduct safety
 +
                                education to us when we first build the team. At the same time, she also supervises
 +
                                the
 +
                                daily
 +
                                hygiene practices of our laboratories. In the conversation with her, we understand the
 +
                                importance of many security systems and their sources. And, she often comes to the lab
 +
                                to
 +
                                see
 +
                                us and cares about our lives.
 +
                                <br><br>
 +
                            </div>
 +
                        </div>
 +
 
 +
                    </div>
 +
                </div>
 +
                <div style="margin: 20px 0;" class="row">
 +
                    <div class="col-xs-12 col-sm-3 col-md-3 col-lg-3">
 +
                        <img style="border:4px solid #E0CCAE; box-shadow: 0 1px 3px #676767; " src="https://static.igem.org/mediawiki/2018/7/77/T--HZAU-China--laoshicao.jpg"
 +
                            width=100% alt="">
 +
                    </div>
 +
                    <div class="col-xs-12 col-sm-9 col-md-9 col-lg-9">
 +
 
 +
                        <div class="collapseDiv">
 +
                            <label for="zhedie-toggle4">Prf. Cao Gang</label>
 +
                            <input type="checkbox" id="zhedie-toggle4" />
 +
                            <div id="zhedie4" class="text-success text-left">
 +
                                <b>Professor of Huazhong Agricultural University, doctoral tutor, head of Department of
 +
                                    Basic Veterinary Science, deputy director of Biomedical Center.</b> <br>
 +
                                In July, we had a nice symposium with Cao. At that time, our team didn’t come up with a
 +
                                mature
 +
                                project. Since Cao is major in Biomedical, we asked him for help and he responded us
 +
                                warmly. In
 +
                                a special symposium, He pointed the weakness is our naïve thoughts and helped us
 +
                                strengthen
 +
                                the
 +
                                advantages. He taught us tremendous things about pyroptosis, including it’s pathway and
 +
                                recent
 +
                                researches in that field, and encouraged us to march on. Cao is so enthusiastic about
 +
                                our
 +
                                project and willing to give us a helping hand.
 +
                                <br><br>
 +
 
 +
                            </div>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
 
 +
 
 +
                <div class="h2"> <a href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Education & Public
 +
                        Engagement</a></div>
 +
                <p>After attending meetings and analyzing questionnaires, we learned that there are many concerns about
 +
                    the use of bacteria to treat cancer. We thought people's scruple is largely because they know
 +
                    little about bacterial therapy and synthetic biology. So, we not only set up a blog for
 +
                    popularizing science about synthetic biology and our project, but also gave lectures to freshmen in
 +
                    our university about our work to get more feedback. Through above activities, we expanded the scope
 +
                    of our survey. By making people more aware of synthetic biology and bacterial therapy, they became
 +
                    more receptive to our project. In the survey results and discussion on our blog, we realized that
 +
                    more knowledge can significantly reduce peoples’ worries. During our education engagement, we also
 +
                    had more safety thinking and better performance in our experimental design.</p>
 +
            </div>
 +
            <div id="float03">
 +
                <div class="h1">Integrated Human Practice</div>
 +
                <p>Pyroptosis therapy, as a new approach derived from cell natural defense mechanism, can bring
 +
                    positive
 +
                    effects to human health with our rightful use. We all know that one day synthetic biologists will
 +
                    develop
 +
                    artificial life forms as vehicles. At that point, pyroptosis carried out by these excellent tools
 +
                    will
 +
                    release its enormous potential, work just like the nanorobots in science fiction movies, leaving
 +
                    cancer cells
 +
                    nowhere to hide. Within the right hands, it can provide us with new opportunities of making the
 +
                    world a
 +
                    better place. </p>
 +
                <div style="width: 70%; margin: 30px auto">
 +
                    <img src="https://static.igem.org/mediawiki/2018/2/25/T--HZAU-China--hp2.png" width=100% alt="">
 +
                </div>
 +
                <p>For a new treatment, a person or institution cannot represent all human beings to define it.
 +
                    Therefore, our integrated human practice aims to hear voices from both experts and the public, in
 +
                    order to analyze and improve our project. At the beginning, we participated in two conference
 +
                    meetings in mainland China, and had an in-depth communication with the teams who had conducted the
 +
                    tumor treatment related projects like us. Then, we consulted several experts from Hubei Cancer
 +
                    Hospital and professors of microbiology and biomedical in our school for their professional advice.
 +
                    Besides the communications, we have done an insightful social survey, to gain better understanding
 +
                    about what people concern about cancer, as well as their attitude towards our project. The social
 +
                    survey results inspired us to reconsider the security level and the future application of our
 +
                    project. Meanwhile, we sensed that another mission for us is to help the public learn more about
 +
                    cancer and its treatment, as well as the difference that synthetic biology can make in future
 +
                    approach. To accomplish that, we held a live seminar to share our idea and then started to blog
 +
                    newly emerged cancer therapies, basic aspects of synthetic biology and our project. It is also
 +
                    a platform, in which we can keep offerring people cancer-related information with a
 +
                    more lucid way to express. We also answered their questions in person for the best effects at
 +
                    comment area.</p>
 +
                <div class="h2">Enhanced Safe Properties</div>
 +
                <p>We carried out our project with full compliance with the safety policies and safety rules (see also
 +
                    <a href="https://2018.igem.org/Team:HZAU-China/Safety">Safety</a> for details). In analyzing the
 +
                    results of expert consultation and public investigation (see
 +
                    also <a href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">Education</a> for details),
 +
                    we concluded that an enhancement of safe properties nevertheless
 +
                    should be considered as a first priority. After a careful reconsideration, we introduced two major
 +
                    safety improvement. First, we knocked out the <i>sifA</i> gene from <i>Salmonella Typhimurium</i>
 +
                    str. SL1344
 +
                    genome by conjugation transfer, in order to weaken its pathogenicity. Although <i>Salmonella</i>
 +
                    has
 +
                    already been used in some cancer therapy research, its pathogenic bacteria identity had limited its
 +
                    anticancer application. After collecting opinions from experts, we decided to perform this gene
 +
                    knock-out. Then we demonstrated the effect of this change by using macrophage assay, where Δ<i>sifA</i>
 +
                    strain cannot replicate effectively in the host cell after infection, in contrast with the control
 +
                    group (see also <a href="https://2018.igem.org/Team:HZAU-China/Design">Design</a> and <a href="https://2018.igem.org/Team:HZAU-China/Results">Results</a>
 +
                    for more information). This effect means the Δ<i>sifA</i> strain can be
 +
                    easily cleaned by human macrophage, which can prevent unexpected infection from occurring. On the
 +
                    other hand, even though <i>Salmonella</i> have natural taxis towards tumor microenvironment and
 +
                    attack
 +
                    tumor cells selectively, any destructions done to the non-tumor tissue remain unacceptable. For the
 +
                    purpose of an accurate attack, we displayed RGD motif on the bacteria surface to demonstrate an
 +
                    enhanced targeting specificity towards tumor cells covered by αvβ3 integrin (see also <a href="https://2018.igem.org/Team:HZAU-China/Design">Design</a>
 +
                    and
 +
                    <a href="https://2018.igem.org/Team:HZAU-China/Results">Results</a> for more information).</p>
 +
 
 +
 
 +
 
 +
 
 +
            </div>
 +
        </div>
 +
        <!-- 侧边 -->
 +
        <div class="cebian">
 +
            <!-- 滚动菜单栏 -->
 +
            <a class="daohangyi" href="https://2018.igem.org/Team:HZAU-China/Safety">
 +
                <span class="biaoti">Safety</span>
 +
                <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/7/7c/T--HZAU-China--ysj.svg" alt=""></span>
 +
            </a>
 +
            <div class="daohangyi">
 +
                <span class="biaoti">Human Practices</span>
 +
                <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/8/8d/T--HZAU-China--xjt.svg" alt=""></span>
 +
            </div>
 +
            <div class="floatCtro">
 +
                <p class="daohanger">Overview</p>
 +
                <p class="daohanger">Silver Human Practice</p>
 +
                <p class="daohanger">Integrated Human Practice</p>
 +
                <a>
 +
                    <span>Back&nbsp;to&nbsp;Top</span>
 +
                </a>
 +
            </div>
 +
            <a class="daohangyi" href="https://2018.igem.org/Team:HZAU-China/Public_Engagement">
 +
                <span class="biaoti">Public Engagement</span>
 +
                <span class="xsjPic"><img src="https://static.igem.org/mediawiki/2018/7/7c/T--HZAU-China--ysj.svg" alt=""></span>
 +
            </a>
 +
            <!-- 滚动菜单栏 -->
 +
        </div>
 +
    </div>
 +
 
 +
    <!-- 滚动菜单栏jQuery -->
 +
    <script src="jquery.js" type="text/javascript"></script>
 +
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 +
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 +
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 +
            $(window).scroll(scrolls)
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            scrolls()
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 +
 
 +
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Latest revision as of 03:39, 18 October 2018

Overview

As the Chinese saying goes, “know the enemy and know yourself, and you can fight a hundred battles with no danger of defeat”. In the battle with cancer, we have gained great knowledge about the “enemy”, as well as our own immune system. However, despite the success in curing early and middle stage cancers we have made, the lack of public comprehension is still likely to cause panic. We sensed that our mission is to help the public learn more about cancer and its treatment, as well as the difference that synthetic biology can make in future approach. To accomplish that, we held a live seminar to share our idea and then started to blog newly emerged cancer therapies, basic aspects of synthetic biology and our project. Meanwhile, letting the public know that their voices have been heard is also very important, so we collected their opinions carefully through public survey, designed new circuits for their safety concerns, and invited them for discussion on our team blog. To explore the further application and the potential risk, we also communicated with some cancer experts. We want to be a communicating bridge during this battle where information is accessible to everyone who needs it.

Silver Human Practice
Safety

This year, we have done a lot of work in order to meet the requirement of biosafety. After collecting opinions from experts and the public, we learned that the general concerns are about: How is it safe to apply bacteriotherapy against cancer without causing unexpected damage or infection? We took these concerns seriously and have found some practical solutions.

EXPERT interviews
College of Life Science and Technology, Huazhong Agricultural University.
Professor Jin He has given us a lot of useful suggestions and instructions. As an expert in microbes, he points out that Salmonella is a kind of safe bacterium which is widely used in researches around the world. After knocking out a series of genes, the harm of Salmonella is acceptable. He also points out that Salmonella has very common with E.coli on behalf that both bacteria are in Enterobacteriaceae. This means that we can incubate Salmonella on the same condition and cultivation with E. coli. In addition, Salmonella is very similar to E. coli in genetics, which means many metabolic reactions happen in E. coli would also happen in Salmonella. Besides, he tells us that there are many intracellular parasites well studied such as Listeria , so we have many choices in the future. In conclusion, his indispensable instructions have made up our minds to engineer on Salmonella.

Doctor of Hubei Tumor Hospital, Wuhan, Hubei Province, PRC.
Doctor Qike Jia is a young doctor works in Hubei Tumor Hospital and has some experiences in tumor treatment. He is familiar with all popular tumor therapies in China and has given us an overview of tumor treatments. When asked about what the most used therapy at present is, the doctor directly pointed out that it is surgery without hesitate. He thinks that operating on patients is always the first thing to do when a doctor is facing a tumor in medium or late stage. After cutting off tumor issues, radiation and chemical therapies can be carried out for further clearance of tumor cells. When asked about the opinion to our project, he encouraged us with a big smile on his face. He said our project is quite good and expectable, although we may have faced some trouble. Cell cultivation is a really hard work taking a lot of time and spirits. Even if we could complete the project, we still need an enormous amount of efforts to push our therapy to the market. He hoped that we could overcome all the difficulties and gain the final success.

HZAU biological experimental teaching center deputy director Dr. Yingchun Qi is one of the people who have the closest connection with our lab. She is majored in cell culture and genetic transformation. Every year, she will conduct safety education to us when we first build the team. At the same time, she also supervises the daily hygiene practices of our laboratories. In the conversation with her, we understand the importance of many security systems and their sources. And, she often comes to the lab to see us and cares about our lives.

Professor of Huazhong Agricultural University, doctoral tutor, head of Department of Basic Veterinary Science, deputy director of Biomedical Center.
In July, we had a nice symposium with Cao. At that time, our team didn’t come up with a mature project. Since Cao is major in Biomedical, we asked him for help and he responded us warmly. In a special symposium, He pointed the weakness is our naïve thoughts and helped us strengthen the advantages. He taught us tremendous things about pyroptosis, including it’s pathway and recent researches in that field, and encouraged us to march on. Cao is so enthusiastic about our project and willing to give us a helping hand.

Education & Public Engagement

After attending meetings and analyzing questionnaires, we learned that there are many concerns about the use of bacteria to treat cancer. We thought people's scruple is largely because they know little about bacterial therapy and synthetic biology. So, we not only set up a blog for popularizing science about synthetic biology and our project, but also gave lectures to freshmen in our university about our work to get more feedback. Through above activities, we expanded the scope of our survey. By making people more aware of synthetic biology and bacterial therapy, they became more receptive to our project. In the survey results and discussion on our blog, we realized that more knowledge can significantly reduce peoples’ worries. During our education engagement, we also had more safety thinking and better performance in our experimental design.

Integrated Human Practice

Pyroptosis therapy, as a new approach derived from cell natural defense mechanism, can bring positive effects to human health with our rightful use. We all know that one day synthetic biologists will develop artificial life forms as vehicles. At that point, pyroptosis carried out by these excellent tools will release its enormous potential, work just like the nanorobots in science fiction movies, leaving cancer cells nowhere to hide. Within the right hands, it can provide us with new opportunities of making the world a better place.

For a new treatment, a person or institution cannot represent all human beings to define it. Therefore, our integrated human practice aims to hear voices from both experts and the public, in order to analyze and improve our project. At the beginning, we participated in two conference meetings in mainland China, and had an in-depth communication with the teams who had conducted the tumor treatment related projects like us. Then, we consulted several experts from Hubei Cancer Hospital and professors of microbiology and biomedical in our school for their professional advice. Besides the communications, we have done an insightful social survey, to gain better understanding about what people concern about cancer, as well as their attitude towards our project. The social survey results inspired us to reconsider the security level and the future application of our project. Meanwhile, we sensed that another mission for us is to help the public learn more about cancer and its treatment, as well as the difference that synthetic biology can make in future approach. To accomplish that, we held a live seminar to share our idea and then started to blog newly emerged cancer therapies, basic aspects of synthetic biology and our project. It is also a platform, in which we can keep offerring people cancer-related information with a more lucid way to express. We also answered their questions in person for the best effects at comment area.

Enhanced Safe Properties

We carried out our project with full compliance with the safety policies and safety rules (see also Safety for details). In analyzing the results of expert consultation and public investigation (see also Education for details), we concluded that an enhancement of safe properties nevertheless should be considered as a first priority. After a careful reconsideration, we introduced two major safety improvement. First, we knocked out the sifA gene from Salmonella Typhimurium str. SL1344 genome by conjugation transfer, in order to weaken its pathogenicity. Although Salmonella has already been used in some cancer therapy research, its pathogenic bacteria identity had limited its anticancer application. After collecting opinions from experts, we decided to perform this gene knock-out. Then we demonstrated the effect of this change by using macrophage assay, where ΔsifA strain cannot replicate effectively in the host cell after infection, in contrast with the control group (see also Design and Results for more information). This effect means the ΔsifA strain can be easily cleaned by human macrophage, which can prevent unexpected infection from occurring. On the other hand, even though Salmonella have natural taxis towards tumor microenvironment and attack tumor cells selectively, any destructions done to the non-tumor tissue remain unacceptable. For the purpose of an accurate attack, we displayed RGD motif on the bacteria surface to demonstrate an enhanced targeting specificity towards tumor cells covered by αvβ3 integrin (see also Design and Results for more information).

Safety
Human Practices

Overview

Silver Human Practice

Integrated Human Practice

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Public Engagement