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{{NEU_China_B}} | {{NEU_China_B}} | ||
<html> | <html> | ||
− | < | + | <style> |
+ | .slider-box{position:relative} | ||
+ | .slider_cube,.slider_inner_a,.slider_inner_b | ||
+ | {position:absolute;width:100%;height:100%;overflow:hidden} | ||
+ | .slider_nav_box{position:absolute;right:0;bottom:25%} | ||
+ | .slider_nav_title{background:url(../../../i1.tietuku.com/87ec913b390798fa.png) repeat center right;min-width:150px;height:100px} | ||
+ | .slider_nav{background:url(http://i1.tietuku.com/87ec913b390798fa.png) no-repeat;height:18px;min-width:100px} | ||
+ | .slider_bar{position:absolute;width:100%;text-align:center;bottom:10px;height:20px} | ||
+ | .slider_bar span{ | ||
+ | display:inline-block;margin:0 10px;width:14px;height:14px; | ||
+ | background:#B7B7B7;cursor:pointer; | ||
+ | border-radius:20px; | ||
+ | box-shadow:inset 0 0 3px rgba(0,0,0,0.3); | ||
+ | -webkit-transition:.3s; | ||
+ | -moz-transition:.3s; | ||
+ | -ms-transition:.3s; | ||
+ | -o-transition:.3s; | ||
+ | transition:.3s | ||
+ | } | ||
+ | .slider_bar span:hover{background:#333;background:rgba(0,0,0,0.7)} | ||
+ | .slider_bar span.active{background:#F40;background:rgba(255,68,0,0.9);cursor:default} | ||
+ | </style> | ||
+ | <script> | ||
+ | jQuery.easing["jswing"]=jQuery.easing["swing"];jQuery.extend(jQuery.easing,{def:"easeOutQuad",swing:function(x,t,b,c,d){return jQuery.easing[jQuery.easing.def](x,t,b,c,d)},easeOutQuad:function(x,t,b,c,d){return -c*(t/=d)*(t-2)+b},easeOutBounce:function(x,t,b,c,d){if((t/=d)<(1/2.75)){return c*(7.5625*t*t)+b}else{if(t<(2/2.75)){return c*(7.5625*(t-=(1.5/2.75))*t+0.75)+b}else{if(t<(2.5/2.75)){return c*(7.5625*(t-=(2.25/2.75))*t+0.9375)+b}else{return c*(7.5625*(t-=(2.625/2.75))*t+0.984375)+b}}}}});String.prototype.format=function(){var args=arguments;var reg=/\{(\d+)\}/g;return this.replace(reg,function(g0,g1){return args[+g1]})};(function($){$.fn.will=function(callBack,type){type=type||"fx";return $.each(this,function(index,ele){$(ele).queue(type,function(next){callBack.call(this);next()})})}})(jQuery);(function($){function Slider(option){this.opt=$.extend(true,{},option);this.ele=$("#"+this.opt.id);this.inIt();this.begin()}Slider.prototype={cubeSize:{x:0,y:0},nowIndex:0,canChose:true,nextIndex:function(){var newIndex=this.nowIndex+1;if(newIndex>=this.opt.imgs.length){newIndex=0}return newIndex},inIt:function(){this.preLoad();this.fill();this.resize();var self=this;$(window).resize(function(){self.resize()})},preLoad:function(){var arr=this.opt.imgs;$.each(arr,function(index,url){var img=document.createElement("img");img.src=url})},begin:function(){var self=this;this.timer=setInterval(function(){var newIndex=self.nowIndex+1;if(newIndex>=self.opt.imgs.length){newIndex=0}self.choseImg(newIndex)},self.opt.interval)},stop:function(){clearInterval(this.timer)},fill:function(){var result='<div class="slider-box">';for(var x=0;x<this.opt.x;x++){for(var y=0;y<this.opt.y;y++){result+='<div id="{0}" class="{1}"><div class="slider_inner_a"></div><div class="slider_inner_b"></div></div>'.format(this.opt.id+"_"+x+"_"+y,"slider_cube")}}if(this.opt.showBar){result+='<div class="slider_bar">';result+=new Array(this.opt.imgs.length+1).join("<span></span>");result+="</div>"}result+="</div>";this.ele.html(result);this.ele.find(".slider_bar span").eq(this.nowIndex).addClass("active");this.eleBox=this.ele.children(".slider-box");this.bindEvents()},bindEvents:function(){var self=this;if(this.opt.showBar){this.eleBox.children(".slider_bar").off().on("click","span",function(){var eleSpan=$(this);self.choseImg(eleSpan.index())})}if(this.opt.urls.length<=0){return}self.eleBox.find(".slider_cube").off().click(function(){window.location.href=self.opt.urls[self.nowIndex]||"javascript:void(0);"}).css("cursor","pointer");self.eleBox.off().mouseenter(function(){self.stop()}).mouseleave(function(){self.begin()})},resize:function(){this.eleBox.height(this.eleBox.width()/this.opt.scale);var eleWid=this.eleBox.width(),eleHei=this.eleBox.height();var xSize,ySize;if(this.opt.border){xSize=(eleWid-this.opt.x+1)/this.opt.x;ySize=(eleHei-this.opt.y+1)/this.opt.y}else{xSize=eleWid/this.opt.x;ySize=eleHei/this.opt.y}this.cubeSize={x:xSize,y:ySize};if(this.opt.x==1){xSize=eleWid}if(this.opt.y==1){ySize=elehei}var borLen=this.opt.border?1:0;for(var x=0;x<this.opt.x;x++){for(var y=0;y<this.opt.y;y++){$("#"+this.opt.id+"_"+x+"_"+y).css({"left":x*xSize+x*borLen+"px","top":y*ySize+y*borLen+"px","width":xSize+"px","height":ySize+"px"}).find(".slider_inner_a,.slider_inner_b").css({"background-image":"url({0})".format(this.opt.imgs[this.nowIndex]),"background-position":"{0}px {1}px".format(-x*xSize,-y*ySize),"background-size":"{0}px {1}px".format(eleWid,eleHei)})}}},choseImg:function(index){var self=this;if(index==self.nowIndex){return}if(!self.canChose){return}self.canChose=false;setTimeout(function(){self.canChose=true},self.opt.delay+200);this.nowIndex=index;var url=this.opt.imgs[index];this.effectIndex=this.effectIndex||0;if(this.effectIndex>=this.effects.length){this.effectIndex=0}this.effects[this.effectIndex](this,url);this.effectIndex++;if(self.opt.showBar){self.eleBox.children(".slider_bar").find("span").removeClass("active").eq(self.nowIndex).addClass("active")}},effects:[function(self,url){var delayItem=self.opt.delay/(self.opt.x*self.opt.y);effectTemplate(self.opt,function(eleA,eleB,x,y){eleA.css({"top":-self.cubeSize.y+"px","background-image":"url({0})".format(url)});var delayTime=(x+(self.opt.x*y))*delayItem*4/5;eleA.delay(delayTime).animate({"top":"0"},{duration:delayItem*10,easing:"easeOutBounce"});eleB.delay(delayTime).animate({"top":self.cubeSize.y+"px"},{duration:delayItem*10,easing:"easeOutBounce"}).will(function(){$(this).css({"top":"0","background-image":"url({0})".format(url)})})})},function(self,url){var delayItem=self.opt.delay/self.opt.x;effectTemplate(self.opt,function(eleA,eleB,x,y){eleA.css({"left":-self.cubeSize.x+"px","background-image":"url({0})".format(url)});var delayTime=x*delayItem;eleA.delay(delayTime).animate({"left":"0"},{duration:delayItem*2});eleB.delay(delayTime).animate({"left":self.cubeSize.x+"px"},{duration:delayItem*2}).will(function(){$(this).css({"left":"0","background-image":"url({0})".format(url)})})})},function(self,url){var delayItem=self.opt.delay/(self.opt.x*self.opt.y); | ||
+ | effectTemplate(self.opt,function(eleA,eleB,x,y){eleA.css({"opacity":"0","background-image":"url({0})".format(url)});var delayTime=(x+(self.opt.x*y))*delayItem;eleA.delay(delayTime).animate({"opacity":"1"},{duration:delayItem*4,});eleB.delay(delayTime).animate({"opacity":"0"},{duration:delayItem*4,}).will(function(){$(this).css({"opacity":"1","background-image":"url({0})".format(url)})})})},function(self,url){var delayArr=[0];var delayItem=self.opt.delay/(self.opt.x*self.opt.y);for(var i=1,len=self.opt.x*self.opt.y;i<len;i++){delayArr[i]=delayArr[i-1]+delayItem}delayArr.sort(function(a,b){return Math.random()>0.5?1:-1});effectTemplate(self.opt,function(eleA,eleB,x,y){var directionIndex=Math.floor(Math.random()*4);var cssName=["top","left","top","left"][directionIndex];var cssValue=[-self.cubeSize.y,self.cubeSize.x,self.cubeSize.y,-self.cubeSize.x][directionIndex];var obj={};obj[cssName]=cssValue+"px";obj["background-image"]="url({0})".format(url);eleA.css(obj);var delayTime=delayArr[x+y*self.opt.x];obj={};obj[cssName]="0";eleA.delay(delayTime*3/4).animate(obj,delayItem*4);obj={};obj[cssName]=-cssValue+"px";eleB.delay(delayTime*3/4).animate(obj,delayItem*4).will(function(){obj={};obj[cssName]="0";obj["background-image"]="url({0})".format(url);$(this).css(obj)})})}]};function effectTemplate(opt,callback){for(var y=0;y<opt.y;y++){for(var x=0;x<opt.x;x++){var eleBox=$("#{0}_{1}_{2}".format(opt.id,x,y));var eleA=eleBox.children(".slider_inner_a");var eleB=eleBox.children(".slider_inner_b");callback(eleA,eleB,x,y)}}}function getRandomId(){return Math.random().toString(36).substr(2).replace(/\d/g,"")}$.fn.slider=function(option,obj){if(typeof option=="object"){var defaults={imgs:[],urls:[],x:2,y:2,scale:4/3,delay:800,interval:5000,border:false};return $.each(this,function(index,ele){var opt=$.extend({},defaults,option);if(!ele.id){ele.id=getRandomId()}opt.id=ele.id;var slider=new Slider(opt);$(ele).data("slider",slider)})}else{if(typeof option=="string"){var slider=this.data("slider");slider.opt=$.extend({},slider.opt,obj||{});slider.inIt();slider.stop();slider.begin();if(typeof obj=="object"){slider[option]()}else{slider[option](obj)}return this}else{return this}}}})(jQuery); | ||
+ | </script> | ||
+ | <div style="margin-bottom: 20px;overflow: hidden;background-color:#fff;height:360px;"> | ||
+ | <div id="imgBar" style="margin:0 0;"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!-- 启动 --> | ||
+ | <script type="text/javascript"> | ||
+ | $("#imgBar").slider({ | ||
+ | imgs: [ | ||
+ | "https://static.igem.org/mediawiki/2018/0/07/T--NEU_China_B--ast0.jpg", | ||
+ | "https://static.igem.org/mediawiki/2018/4/41/T--NEU_China_B--ast1.jpg", | ||
+ | "https://static.igem.org/mediawiki/2018/9/92/T--NEU_China_B--ast2.jpg"], | ||
+ | scale: 4, | ||
+ | border: true, | ||
+ | delay: 2200, | ||
+ | x: 10, | ||
+ | y: 3 | ||
+ | }); | ||
+ | </script> | ||
+ | |||
+ | <canvas id="base_page" width="800" height="400" style=""> | ||
您的浏览器不支持 HTML5 canvas 标签。 | 您的浏览器不支持 HTML5 canvas 标签。 | ||
</canvas> | </canvas> | ||
<script> | <script> | ||
− | + | var width = 800; | |
− | + | var height = 400; | |
− | + | var c = document.getElementById("base_page"); | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | $("#base_page").attr("width", c.parentElement.clientWidth); | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | var ctx = c.getContext("2d"); | |
+ | that = ctx; | ||
+ | var i = 0; | ||
+ | var pi = Math.PI; | ||
+ | var basex = c.width / 2; | ||
+ | var basey = c.height / 2; | ||
+ | width = c.width; | ||
+ | height = c.height; | ||
+ | var imgx; | ||
+ | var imgy; | ||
+ | |||
+ | var w1 = "https://static.igem.org/mediawiki/2018/a/a8/T--NEU_China_B--w1.png"; | ||
+ | var w2 = "https://static.igem.org/mediawiki/2018/f/f9/T--NEU_China_B--w2.png"; | ||
+ | var w3 = "https://static.igem.org/mediawiki/2018/2/27/T--NEU_China_B--w3.png"; | ||
+ | var w4 = "https://static.igem.org/mediawiki/2018/9/96/T--NEU_China_B--w4.png"; | ||
+ | var w5 = "https://static.igem.org/mediawiki/2018/7/7a/T--NEU_China_B--w5.png"; | ||
+ | var w6 = "https://static.igem.org/mediawiki/2018/6/66/T--NEU_China_B--w6.png"; | ||
+ | |||
+ | var img1 = new Image(); | ||
+ | var img2 = new Image(); | ||
+ | var img3 = new Image(); | ||
+ | var img4 = new Image(); | ||
+ | var img5 = new Image(); | ||
+ | var img6 = new Image(); | ||
+ | img1.src = w1; | ||
+ | var during = 40; | ||
+ | var t1 = 0; | ||
+ | var base_t1 = 0; | ||
+ | var t2 = 0; | ||
+ | var base_t2 = base_t1 + during; | ||
+ | var t3 = 0; | ||
+ | var base_t3 = base_t2 + during; | ||
+ | var t4 = 0; | ||
+ | var base_t4 = base_t3 + during; | ||
+ | var t5 = 0; | ||
+ | var base_t5 = base_t4 + during; | ||
+ | var t6 = 0; | ||
+ | var base_t6 = base_t5 + during; | ||
+ | |||
+ | var x_e = 300; | ||
+ | var y_e = 150; | ||
+ | |||
+ | var tipsWarpper = {}; | ||
+ | |||
+ | |||
+ | function redraw() { | ||
+ | ctx.clearRect(0, 0, width, height); | ||
+ | |||
+ | var i = 0; | ||
+ | ctx.beginPath(); | ||
+ | var x = x_e * Math.cos(0 * 180 * 2) + basex; | ||
+ | var y = y_e * Math.sin(0 * 180 * 4) + basey; | ||
+ | ctx.moveTo(x, y); | ||
+ | for (i = 0; i < 0.05; i += 0.00001) { | ||
+ | var x = x_e * Math.cos(i * 180 * 2) + basex; | ||
+ | var y = y_e * Math.sin(i * 180 * 4) + basey; | ||
+ | ctx.lineTo(x, y); | ||
+ | } | ||
+ | ctx.strokeStyle = '#FAF0E6'; | ||
+ | ctx.lineWidth = 3; | ||
+ | ctx.stroke(); | ||
+ | ctx.closePath(); | ||
+ | |||
+ | } | ||
+ | |||
+ | function draw(x, y, r) { | ||
+ | ctx.beginPath(); | ||
+ | ctx.arc(x, y, r, 0, Math.PI * 2, true); | ||
+ | ctx.fillStyle = "red"; | ||
+ | ctx.fill(); | ||
+ | ctx.closePath(); | ||
+ | } | ||
+ | function drawTips(x, y, w, h, msg) { | ||
+ | // ctx.beginPath(); | ||
+ | // ctx.rect(x, y, w, h); | ||
+ | // ctx.fillStyle = 'white'; | ||
+ | // ctx.fill(); | ||
+ | // ctx.closePath(); | ||
+ | ctx.beginPath(); | ||
+ | ctx.font = 'italic 40pt Calibri'; | ||
+ | ctx.fillStyle = 'yellow'; | ||
+ | ctx.fillText(msg, x, y); | ||
+ | ctx.fill(); | ||
+ | ctx.closePath(); | ||
+ | } | ||
+ | |||
+ | function drawImg(imgWarpper, x, y) { | ||
+ | var img = imgWarpper.img; | ||
+ | var drawWidth = img.width / 4; | ||
+ | var drawHeight = img.height / 4; | ||
+ | var imx = x - drawWidth / 2; | ||
+ | var imy = y - drawHeight / 2 | ||
+ | imgWarpper.x = imx; | ||
+ | imgWarpper.y = imy; | ||
+ | ctx.drawImage(img, imx, imy, drawWidth, drawHeight); | ||
+ | } | ||
+ | |||
+ | var imgWarpper1 = {}; | ||
+ | var imgWarpper2 = {}; | ||
+ | var imgWarpper3 = {}; | ||
+ | var imgWarpper4 = {}; | ||
+ | var imgWarpper5 = {}; | ||
+ | var imgWarpper6 = {}; | ||
+ | |||
+ | imgWarpper1.img = img1; | ||
+ | imgWarpper2.img = img2; | ||
+ | imgWarpper3.img = img3; | ||
+ | imgWarpper4.img = img4; | ||
+ | imgWarpper5.img = img5; | ||
+ | imgWarpper6.img = img6; | ||
+ | |||
+ | img1.onload = function () { | ||
+ | img2.src = w2; | ||
+ | img2.onload = function () { | ||
+ | img3.src = w3; | ||
+ | img3.onload = function () { | ||
+ | img4.src = w4; | ||
+ | img4.onload = function () { | ||
+ | img5.src = w5; | ||
+ | img5.onload = function () { | ||
+ | var timer = setInterval(function () { | ||
+ | redraw(); | ||
+ | i += 0.00001; | ||
+ | |||
+ | t1 = i + base_t1; | ||
+ | t2 = i + base_t2; | ||
+ | t3 = i + base_t3; | ||
+ | t4 = i + base_t4; | ||
+ | t5 = i + base_t5; | ||
+ | |||
+ | var img1_x = x_e * Math.cos(t1 * 180 * 2) + basex; | ||
+ | var img1_y = y_e * Math.sin(t1 * 180 * 4) + basey; | ||
+ | |||
+ | drawImg(imgWarpper1, img1_x, img1_y); | ||
+ | |||
+ | var img2_x = x_e * Math.cos(t2 * 180 * 2) + basex; | ||
+ | var img2_y = y_e * Math.sin(t2 * 180 * 4) + basey; | ||
+ | imgWarpper2.x = img2_x; | ||
+ | imgWarpper2.y = img2_y; | ||
+ | // imgWarpper2.w = i | ||
+ | drawImg(imgWarpper2, img2_x, img2_y); | ||
+ | |||
+ | |||
+ | var img3_x = x_e * Math.cos(t3 * 180 * 2) + basex; | ||
+ | var img3_y = y_e * Math.sin(t3 * 180 * 4) + basey; | ||
+ | drawImg(imgWarpper3, img3_x, img3_y); | ||
+ | |||
+ | var img4_x = x_e * Math.cos(t4 * 180 * 2) + basex; | ||
+ | var img4_y = y_e * Math.sin(t4 * 180 * 4) + basey; | ||
+ | drawImg(imgWarpper4, img4_x, img4_y); | ||
+ | |||
+ | |||
+ | var img5_x = x_e * Math.cos(t5 * 180 * 2) + basex; | ||
+ | var img5_y = y_e * Math.sin(t5 * 180 * 4) + basey; | ||
+ | drawImg(imgWarpper5, img5_x, img5_y); | ||
+ | |||
+ | |||
+ | if (tipsWarpper.valid == true) | ||
+ | drawTips(tipsWarpper.x, tipsWarpper.y, 100, 100, tipsWarpper.msg); | ||
+ | // if (y > 400) { | ||
+ | // clearInterval(timer); | ||
+ | // } | ||
+ | }, 50); | ||
+ | } | ||
+ | } | ||
+ | } | ||
+ | } | ||
+ | } | ||
+ | |||
+ | function contains(imgWarpper, x, y) { | ||
+ | var img = imgWarpper.img; | ||
+ | var rect = {}; | ||
+ | var drawWidth = img.width / 4; | ||
+ | var drawHeight = img.height / 4; | ||
+ | rect.x = imgWarpper.x; | ||
+ | rect.y = imgWarpper.y;; | ||
+ | rect.x2 = drawWidth + rect.x; | ||
+ | rect.y2 = drawHeight + rect.y; | ||
+ | if (rect.x < x) | ||
+ | if (rect.x2 > x) | ||
+ | if (rect.y < y) | ||
+ | if (rect.y2 > y) | ||
+ | return true; | ||
+ | return false; | ||
+ | } | ||
+ | |||
+ | c.addEventListener('click', function (event) { | ||
+ | var x = event.clientX - c.getBoundingClientRect().left; | ||
+ | var y = event.clientY - c.getBoundingClientRect().top; | ||
+ | // console.log(x, y); | ||
+ | if (contains(imgWarpper1, x, y)) { | ||
+ | console.log("img 1"); | ||
+ | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/background'; | ||
+ | } | ||
+ | else if (contains(imgWarpper2, x, y)) { | ||
+ | console.log("img 2"); | ||
+ | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Basic_Part'; | ||
+ | } | ||
+ | else if (contains(imgWarpper3, x, y)) { | ||
+ | console.log("img3"); | ||
+ | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Team'; | ||
+ | } | ||
+ | else if (contains(imgWarpper4, x, y)) { | ||
+ | console.log("img4"); | ||
+ | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/InterLab'; | ||
+ | } | ||
+ | else if (contains(imgWarpper5, x, y)) { | ||
+ | console.log("img5"); | ||
+ | window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Human_Practices'; | ||
+ | } | ||
+ | }); | ||
+ | c.onmousemove = function (event) { | ||
+ | var x = event.clientX - c.getBoundingClientRect().left; | ||
+ | var y = event.clientY - c.getBoundingClientRect().top; | ||
+ | if (contains(imgWarpper1, x, y)) { | ||
+ | tipsWarpper.x = x; | ||
+ | tipsWarpper.y = y; | ||
+ | tipsWarpper.valid = true; | ||
+ | tipsWarpper.msg = "Background"; | ||
+ | } | ||
+ | else if (contains(imgWarpper2, x, y)) { | ||
+ | console.log("img 2"); | ||
+ | tipsWarpper.x = x; | ||
+ | tipsWarpper.y = y; | ||
+ | tipsWarpper.valid = true; | ||
+ | tipsWarpper.msg = "Basic Part"; | ||
+ | } | ||
+ | else if (contains(imgWarpper3, x, y)) { | ||
+ | console.log("img3"); | ||
+ | tipsWarpper.valid = true; | ||
+ | tipsWarpper.x = x; | ||
+ | tipsWarpper.y = y; | ||
+ | tipsWarpper.msg = "Team"; | ||
+ | } | ||
+ | else if (contains(imgWarpper4, x, y)) { | ||
+ | console.log("img4"); | ||
+ | tipsWarpper.x = x; | ||
+ | tipsWarpper.y = y; | ||
+ | tipsWarpper.msg = "InterLab"; | ||
+ | tipsWarpper.valid = true; | ||
+ | } | ||
+ | else if (contains(imgWarpper5, x, y)) { | ||
+ | console.log("img5"); | ||
+ | tipsWarpper.x = x; | ||
+ | tipsWarpper.y = y; | ||
+ | tipsWarpper.msg = "Human_Practices"; | ||
+ | tipsWarpper.valid = true; | ||
+ | } | ||
+ | else { | ||
+ | tipsWarpper.valid = false; | ||
+ | } | ||
+ | } | ||
+ | </script> | ||
+ | |||
+ | |||
<hr> | <hr> | ||
− | <div class="text-center"> | + | <style> |
+ | .abstract{ | ||
+ | background-color:#FAF0E6; | ||
+ | }; | ||
+ | </style> | ||
+ | <div class="text-center abstract"> | ||
+ | <div class="container"> | ||
<h2> | <h2> | ||
Abstract | Abstract | ||
</h2> | </h2> | ||
+ | <hr> | ||
The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing. | The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing. | ||
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Latest revision as of 03:39, 18 October 2018
Abstract
The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing.