Difference between revisions of "Team:NEU China B"

 
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{{NEU_China_B}}
 
{{NEU_China_B}}
 
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<html>
 +
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.slider-box{position:relative}
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.slider_cube,.slider_inner_a,.slider_inner_b
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{position:absolute;width:100%;height:100%;overflow:hidden}
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.slider_nav_box{position:absolute;right:0;bottom:25%}
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.slider_nav_title{background:url(../../../i1.tietuku.com/87ec913b390798fa.png) repeat center right;min-width:150px;height:100px}
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.slider_nav{background:url(http://i1.tietuku.com/87ec913b390798fa.png) no-repeat;height:18px;min-width:100px}
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.slider_bar{position:absolute;width:100%;text-align:center;bottom:10px;height:20px}
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.slider_bar span{
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    box-shadow:inset 0 0 3px rgba(0,0,0,0.3);
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    -webkit-transition:.3s;
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    -moz-transition:.3s;
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    -ms-transition:.3s;
 +
    -o-transition:.3s;
 +
    transition:.3s
 +
}
 +
.slider_bar span:hover{background:#333;background:rgba(0,0,0,0.7)}
 +
.slider_bar span.active{background:#F40;background:rgba(255,68,0,0.9);cursor:default}
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</style>
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<script>
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 +
</script>
 +
<div style="margin-bottom: 20px;overflow: hidden;background-color:#fff;height:360px;">
 +
        <div id="imgBar" style="margin:0 0;">
 +
        </div>
 +
    </div>
 +
<!-- 启动 -->
 +
<script type="text/javascript">
 +
    $("#imgBar").slider({
 +
        imgs: [
 +
            "https://static.igem.org/mediawiki/2018/0/07/T--NEU_China_B--ast0.jpg",
 +
"https://static.igem.org/mediawiki/2018/4/41/T--NEU_China_B--ast1.jpg",
 +
"https://static.igem.org/mediawiki/2018/9/92/T--NEU_China_B--ast2.jpg"],
 +
        scale: 4,
 +
        border: true,
 +
        delay: 2200,
 +
        x: 10,
 +
        y: 3
 +
    });
 +
</script>
 +
 +
<canvas id="base_page" width="800" height="400" style="">
 +
您的浏览器不支持 HTML5 canvas 标签。
 +
</canvas>
 +
<script>
 +
var width = 800;
 +
var height = 400;
  
 +
var c = document.getElementById("base_page");
  
−
project description
+
$("#base_page").attr("width", c.parentElement.clientWidth);
−
Wine is the most common drink in people's lives. However, during the production of alcohol, lactic acid is often produced, which not only affects the taste of the wine, but also inhibits the viability of the fermenting yeast, significantly reducing the efficiency of alcohol fermentation. Therefore, NEU China B is dedicated to the construction of a lactic acid biosensor that uses E. coli's group-sensing effect to sense lactic acid during alcohol fermentation and monitor it in real time. When the lactic acid content reaches its peak, it will alarm.
+
  
 +
var ctx = c.getContext("2d");
 +
that = ctx;
 +
var i = 0;
 +
var pi = Math.PI;
 +
var basex = c.width / 2;
 +
var basey = c.height / 2;
 +
width = c.width;
 +
height = c.height;
 +
var imgx;
 +
var imgy;
  
 +
var w1 = "https://static.igem.org/mediawiki/2018/a/a8/T--NEU_China_B--w1.png";
 +
var w2 = "https://static.igem.org/mediawiki/2018/f/f9/T--NEU_China_B--w2.png";
 +
var w3 = "https://static.igem.org/mediawiki/2018/2/27/T--NEU_China_B--w3.png";
 +
var w4 = "https://static.igem.org/mediawiki/2018/9/96/T--NEU_China_B--w4.png";
 +
var w5 = "https://static.igem.org/mediawiki/2018/7/7a/T--NEU_China_B--w5.png";
 +
var w6 = "https://static.igem.org/mediawiki/2018/6/66/T--NEU_China_B--w6.png";
  
−
<div class="column full_size" >
+
var img1 = new Image();
−
<h1> Welcome to iGEM 2018! </h1>
+
var img2 = new Image();
−
<p>Your team has been approved and you are ready to start the iGEM season! </p>
+
var img3 = new Image();
 +
var img4 = new Image();
 +
var img5 = new Image();
 +
var img6 = new Image();
 +
img1.src = w1;
 +
var during = 40;
 +
var t1 = 0;
 +
var base_t1 = 0;
 +
var t2 = 0;
 +
var base_t2 = base_t1 + during;
 +
var t3 = 0;
 +
var base_t3 = base_t2 + during;
 +
var t4 = 0;
 +
var base_t4 = base_t3 + during;
 +
var t5 = 0;
 +
var base_t5 = base_t4 + during;
 +
var t6 = 0;
 +
var base_t6 = base_t5 + during;
  
 +
var x_e = 300;
 +
var y_e = 150;
  
−
<img src="http://placehold.it/1080x320/c4baba/e4dede">
+
var tipsWarpper = {};
−
+
  
−
</div>
 
  
 +
function redraw() {
 +
ctx.clearRect(0, 0, width, height);
  
−
<div class="column full_size" >
+
var i = 0;
 +
ctx.beginPath();
 +
var x = x_e * Math.cos(0 * 180 * 2) + basex;
 +
var y = y_e * Math.sin(0 * 180 * 4) + basey;
 +
ctx.moveTo(x, y);
 +
for (i = 0; i < 0.05; i += 0.00001) {
 +
var x = x_e * Math.cos(i * 180 * 2) + basex;
 +
var y = y_e * Math.sin(i * 180 * 4) + basey;
 +
ctx.lineTo(x, y);
 +
}
 +
ctx.strokeStyle = '#FAF0E6';
 +
ctx.lineWidth = 3;
 +
ctx.stroke();
 +
ctx.closePath();
  
−
<h3>Before you start</h3>
+
}
−
<p> Please read the following pages:</p>
+
−
<ul>
+
−
<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
+
−
<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
+
−
<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
+
−
</ul>
+
−
</div>
+
  
 +
function draw(x, y, r) {
 +
ctx.beginPath();
 +
ctx.arc(x, y, r, 0, Math.PI * 2, true);
 +
ctx.fillStyle = "red";
 +
ctx.fill();
 +
ctx.closePath();
 +
}
 +
function drawTips(x, y, w, h, msg) {
 +
// ctx.beginPath();
 +
// ctx.rect(x, y, w, h);
 +
// ctx.fillStyle = 'white';
 +
// ctx.fill();
 +
// ctx.closePath();
 +
ctx.beginPath();
 +
ctx.font = 'italic 40pt Calibri';
 +
ctx.fillStyle = 'yellow';
 +
ctx.fillText(msg, x, y);
 +
ctx.fill();
 +
ctx.closePath();
 +
}
  
−
<div class="clear extra_space"></div>
+
function drawImg(imgWarpper, x, y) {
−
<div class="line_divider"></div>
+
var img = imgWarpper.img;
−
<div class="clear extra_space"></div>
+
var drawWidth = img.width / 4;
 +
var drawHeight = img.height / 4;
 +
var imx = x - drawWidth / 2;
 +
var imy = y - drawHeight / 2
 +
imgWarpper.x = imx;
 +
imgWarpper.y = imy;
 +
ctx.drawImage(img, imx, imy, drawWidth, drawHeight);
 +
}
  
 +
var imgWarpper1 = {};
 +
var imgWarpper2 = {};
 +
var imgWarpper3 = {};
 +
var imgWarpper4 = {};
 +
var imgWarpper5 = {};
 +
var imgWarpper6 = {};
  
 +
imgWarpper1.img = img1;
 +
imgWarpper2.img = img2;
 +
imgWarpper3.img = img3;
 +
imgWarpper4.img = img4;
 +
imgWarpper5.img = img5;
 +
imgWarpper6.img = img6;
  
−
<div class="column full_size" >
+
img1.onload = function () {
−
<h3> Styling your wiki </h3>
+
img2.src = w2;
−
<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
+
img2.onload = function () {
−
<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
+
img3.src = w3;
 +
img3.onload = function () {
 +
img4.src = w4;
 +
img4.onload = function () {
 +
img5.src = w5;
 +
img5.onload = function () {
 +
var timer = setInterval(function () {
 +
redraw();
 +
i += 0.00001;
  
−
</div>
+
t1 = i + base_t1;
 +
t2 = i + base_t2;
 +
t3 = i + base_t3;
 +
t4 = i + base_t4;
 +
t5 = i + base_t5;
  
 +
var img1_x = x_e * Math.cos(t1 * 180 * 2) + basex;
 +
var img1_y = y_e * Math.sin(t1 * 180 * 4) + basey;
  
 +
drawImg(imgWarpper1, img1_x, img1_y);
  
 +
var img2_x = x_e * Math.cos(t2 * 180 * 2) + basex;
 +
var img2_y = y_e * Math.sin(t2 * 180 * 4) + basey;
 +
imgWarpper2.x = img2_x;
 +
imgWarpper2.y = img2_y;
 +
// imgWarpper2.w = i
 +
drawImg(imgWarpper2, img2_x, img2_y);
  
−
<div class="clear extra_space"></div>
 
  
 +
var img3_x = x_e * Math.cos(t3 * 180 * 2) + basex;
 +
var img3_y = y_e * Math.sin(t3 * 180 * 4) + basey;
 +
drawImg(imgWarpper3, img3_x, img3_y);
  
 +
var img4_x = x_e * Math.cos(t4 * 180 * 2) + basex;
 +
var img4_y = y_e * Math.sin(t4 * 180 * 4) + basey;
 +
drawImg(imgWarpper4, img4_x, img4_y);
  
−
<div class="column third_size" >
 
  
−
<h3> Uploading pictures and files </h3>
+
var img5_x = x_e * Math.cos(t5 * 180 * 2) + basex;
−
<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
+
var img5_y = y_e * Math.sin(t5 * 180 * 4) + basey;
 +
drawImg(imgWarpper5, img5_x, img5_y);
  
  
−
<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
+
if (tipsWarpper.valid == true)
 +
drawTips(tipsWarpper.x, tipsWarpper.y, 100, 100, tipsWarpper.msg);
 +
// if (y > 400) {
 +
// clearInterval(timer);
 +
// }
 +
}, 50);
 +
}
 +
}
 +
}
 +
}
 +
}
  
−
<div class="button_link">
+
function contains(imgWarpper, x, y) {
−
<a href="https://2018.igem.org/Special:Upload">
+
var img = imgWarpper.img;
−
UPLOAD FILES
+
var rect = {};
−
</a>
+
var drawWidth = img.width / 4;
−
</div>
+
var drawHeight = img.height / 4;
 +
rect.x = imgWarpper.x;
 +
rect.y = imgWarpper.y;;
 +
rect.x2 = drawWidth + rect.x;
 +
rect.y2 = drawHeight + rect.y;
 +
if (rect.x < x)
 +
if (rect.x2 > x)
 +
if (rect.y < y)
 +
if (rect.y2 > y)
 +
return true;
 +
return false;
 +
}
  
−
</div>
+
c.addEventListener('click', function (event) {
 +
var x = event.clientX - c.getBoundingClientRect().left;
 +
var y = event.clientY - c.getBoundingClientRect().top;
 +
// console.log(x, y);
 +
if (contains(imgWarpper1, x, y)) {
 +
console.log("img 1");
 +
window.location.href = 'https://2018.igem.org/Team:NEU_China_B/background';
 +
}
 +
else if (contains(imgWarpper2, x, y)) {
 +
console.log("img 2");
 +
window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Basic_Part';
 +
}
 +
else if (contains(imgWarpper3, x, y)) {
 +
console.log("img3");
 +
window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Team';
 +
}
 +
else if (contains(imgWarpper4, x, y)) {
 +
console.log("img4");
 +
window.location.href = 'https://2018.igem.org/Team:NEU_China_B/InterLab';
 +
}
 +
else if (contains(imgWarpper5, x, y)) {
 +
console.log("img5");
 +
window.location.href = 'https://2018.igem.org/Team:NEU_China_B/Human_Practices';
 +
}
 +
});
 +
c.onmousemove = function (event) {
 +
var x = event.clientX - c.getBoundingClientRect().left;
 +
var y = event.clientY - c.getBoundingClientRect().top;
 +
if (contains(imgWarpper1, x, y)) {
 +
tipsWarpper.x = x;
 +
tipsWarpper.y = y;
 +
tipsWarpper.valid = true;
 +
tipsWarpper.msg = "Background";
 +
}
 +
else if (contains(imgWarpper2, x, y)) {
 +
console.log("img 2");
 +
tipsWarpper.x = x;
 +
tipsWarpper.y = y;
 +
tipsWarpper.valid = true;
 +
tipsWarpper.msg = "Basic Part";
 +
}
 +
else if (contains(imgWarpper3, x, y)) {
 +
console.log("img3");
 +
tipsWarpper.valid = true;
 +
tipsWarpper.x = x;
 +
tipsWarpper.y = y;
 +
tipsWarpper.msg = "Team";
 +
}
 +
else if (contains(imgWarpper4, x, y)) {
 +
console.log("img4");
 +
tipsWarpper.x = x;
 +
tipsWarpper.y = y;
 +
tipsWarpper.msg = "InterLab";
 +
tipsWarpper.valid = true;
 +
}
 +
else if (contains(imgWarpper5, x, y)) {
 +
console.log("img5");
 +
tipsWarpper.x = x;
 +
tipsWarpper.y = y;
 +
tipsWarpper.msg = "Human_Practices";
 +
tipsWarpper.valid = true;
 +
}
 +
else {
 +
tipsWarpper.valid = false;
 +
}
 +
}
 +
</script>
 +
 +
 +
<hr>
 +
<style>
 +
.abstract{
 +
background-color:#FAF0E6;
 +
};
 +
</style>
 +
<div class="text-center abstract">
 +
<div class="container">
 +
<h2>
 +
Abstract
 +
</h2>
 +
<hr>
 +
The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing.
  
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<div class="column third_size" >
 
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<h3> Wiki template information </h3>
 
−
<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
 
  
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</div>
 
  
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<div class="column third_size" >
 
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<div class="highlight decoration_B_full">
 
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<h3> Editing your wiki </h3>
 
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
 
−
<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
 
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<div class="button_link">
 
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<a href="https://2018.igem.org/wiki/index.php?title=Team:NEU_China_B&action=edit">
 
−
EDIT PAGE
 
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</a>
 
 
</div>
 
</div>
 +
<footer id="footer" class="footer section">
 +
<!-- Footer Top -->
 +
<div class="footer-top">
 +
<div class="container">
 +
<div class="row">
  
 +
</div>
 +
</div>
 +
</div>
 +
<!--/ End Footer Top -->
  
−
</div>
+
<!-- Copyright -->
−
</div>
+
<div class="copyright">
 +
<div class="container">
 +
<div class="row">
 +
<div class="copyright-content">
  
 +
<div class="row">
  
 +
<div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/2/28/T--NEU_China_B--tb1.png/799px-T--NEU_China_B--tb1.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/6/60/T--NEU_China_B--tb0.png/800px-T--NEU_China_B--tb0.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/5/52/T--NEU_China_B--tb3.png" alt="" class="sr">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/4/4f/T--NEU_China_B--tb2.png" alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/4/48/T--NEU_China_B--tb4.png" alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/b/be/T--NEU_China_B--tb5.png/799px-T--NEU_China_B--tb5.png"
 +
alt="" class="src">
  
 +
</div>
  
 +
<div class="col-xs-6 col-sm-6 col-md-6 col-lg-6">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/b/b9/T--NEU_China_B--tb6.png/320px-T--NEU_China_B--tb6.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/d/d9/T--NEU_China_B--tb7.png/320px-T--NEU_China_B--tb7.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/4/4a/T--NEU_China_B--tb8.png/320px-T--NEU_China_B--tb8.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/4/47/T--NEU_China_B--tb9.png/799px-T--NEU_China_B--tb9.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/2/2d/T--NEU_China_B--tb10.png/202px-T--NEU_China_B--tb10.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/thumb/b/be/T--NEU_China_B--tb5.png/320px-T--NEU_China_B--tb5.png"
 +
alt="" class="src">
 +
<img height=20 src="https://static.igem.org/mediawiki/2018/4/4f/T--NEU_China_B--tb2.png" alt="" class="src">
  
−
<div class="clear extra_space"></div>
+
</div>
−
<div class="line_divider"></div>
+
−
<div class="clear extra_space"></div>
+
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+
−
 
+
−
 
+
−
<div class="column two_thirds_size" >
+
−
<h3>Tips</h3>
+
−
<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
+
−
<ul>
+
−
<li>State your accomplishments! Tell people what you have achieved from the start. </li>
+
−
<li>Be clear about what you are doing and how you plan to do this.</li>
+
−
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
+
−
<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
+
−
<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
+
−
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
+
−
<li>Have lots of fun! </li>
+
−
</ul>
+
−
</div>
+
−
 
+
−
 
+
−
<div class="column third_size">
+
−
<div class="highlight decoration_A_full">
+
−
<h3>Inspiration</h3>
+
−
<p> You can also view other team wikis for inspiration! Here are some examples:</p>
+
−
<ul>
+
−
<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
+
−
<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
+
−
<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
+
−
<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
+
−
</ul>
+
−
</div>
+
−
</div>
+
−
 
+
  
 +
</div>
  
 +
</div>
 +
<ul class="social">
 +
</ul>
 +
</div>
 +
</div>
 +
</div>
 +
<!--/ End Copyright -->
 +
</footer>
  
 
</html>
 
</html>

Latest revision as of 03:39, 18 October 2018

Ruby - Responsive Corporate Tempalte

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Abstract

The role of L-lactate is not always beneficial for the yogurt fermentation due to excessive L-lactate can provide an optimized growth condition for yeast and mold. Therefore, it is important to detect the concentration of L-lactate. Acid-base titration is a common method for it, but this method is complicated and time-consuming. In order to monitor L-lactate quickly and conveniently, we designed a biosensor for detecting L-lactate concentration by using the lldPRD L-lactate operon and QS system in E. coli. One of these parts is able to induce the lldPRD genes expression, LuxS protein, in the presence of L-lactate. LuxS protein catalyzes the SAM cycle and produces a small signaling molecule AI-2 that motivates our second part promoter of LsrA&K to promote GFP expression. The optic fiber is able to detect the GFP signal and convert it into current. Simultaneously, the entire device container will be made by 3D printing.