Difference between revisions of "Team:FSU/Description"

 
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<html>
  
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<!--------------------------- FIRST SECTION --------------------------->
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<h1 style="padding-bottom: 15px;margin-top: 10px;color:#540115;"> OVERVIEW </h1>
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<img src="https://static.igem.org/mediawiki/2018/3/30/T--FSU--djcoli-garnet.png" style="width:7em;">
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<p>
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It is routine to trigger the production of a protein in engineered E. coli using a small molecule such as arabinose, lactose, and rhamnose.  Our project is focused on answering the question, “Can sound be used to induce gene expression in engineered E. coli?”  Prior iGEM projects demonstrated that sound can be used to activate eukaryotic cells.  Review of the scientific literature revealed projects where exposing E. coli to sound correlated with more vigorous growth in cell cultures and increased production of proteins.
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<img src="https://static.igem.org/mediawiki/2018/0/00/T--FSU--speaker-garnet.png" style="width:8em;float:right;padding-left: 1em;">
 +
<p>
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In 2008, the University of California, Berkeley (UC Berkeley) iGEM team contributed five genetic parts to the iGEM community.  The genetic parts were derived from DNA sequences found upstream from genes that were potentially upregulated when the cells were exposed to sound.  The UC Berkeley iGEM team was unable to demonstrate definitively that the genetic parts could be used as sound inducible promoters of gene expression.  We decided to build on their work.  We designed, built, and tested new genetic devices using the Berkeley promoters that were intended to detect sound and stimulate the expression of reporter proteins.
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</p>
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</div>
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<div class="w3-row">
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<img src="https://static.igem.org/mediawiki/2018/8/84/T--FSU--dancer01-garnet.png" style="width:4em;float:left;padding-right:1em">
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<p>
 +
Members of the FSU iGEM team also investigated genes that were up-regulated when exposed to sound, according to other scientific literature. These signaling pathways and promoters were then analyzed and used to build new genetic devices which included reporter proteins that signal different levels of expression. Our new cells were tested at different sound frequencies and amplitudes to see which cells reported the highest level of reporter protein expression.
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</p><p>
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</p></div>
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<div class="w3-row">
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<img src="https://static.igem.org/mediawiki/2018/9/97/T--FSU--speakerHomie.png" style="width:8em;float:right;padding-left:1em;">
 +
<p>
 +
Audio induction has many possible impacts, the limit to which is still unclear.  Our Human Practices team started a discussion in the fields of molecular biology, brewery,  and law enforcement to explore the potential positive and negative impacts of using sound to induce gene expression.
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                                 <h2 class="md-title" style="text-align: center;">Project Description</h2>
 
                                 <h2 class="md-title" style="text-align: center;">Project Description</h2>
 
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                                     <p><b>Question</b><br> Can sound be used to induce <b>gene expression</b> in engineered E. coli?</p>  
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                                     <p><b>Question</b><br> Can sound be used to induce gene expression in engineered E. coli?</p>  
 
                                     <p><b>Approach</b><br> Today, it is routine to induce the expression of a gene in E. coli using a small molecule.  Examples are arabinose, lactose, and rhamnose.  Can sound become a routine means to induce gene expression in engineered E. coli?  Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question but in mammalian cells.  We are studying the details of both projects.  We are characterizing parts submitted by the 2008 UC Berkeley team that are putative promoters at the end of a signal transduction pathway that responds to sound.  In parallel, we are studying genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound.  We will identify promoters that seem to be induced by pathways that respond to sound.  The selected promoters will be used to design new genetic devices that will generate chromogenic or fluorescent reporters when a cell is exposed to sounds.  We will use the new genetic devices to correlate sound frequencies, amplitudes, and exposure times with levels of gene expression.</p>
 
                                     <p><b>Approach</b><br> Today, it is routine to induce the expression of a gene in E. coli using a small molecule.  Examples are arabinose, lactose, and rhamnose.  Can sound become a routine means to induce gene expression in engineered E. coli?  Slovenia’s Sonicell and SUS Tech’s Cearll’s Secret projects pursued a similar question but in mammalian cells.  We are studying the details of both projects.  We are characterizing parts submitted by the 2008 UC Berkeley team that are putative promoters at the end of a signal transduction pathway that responds to sound.  In parallel, we are studying genes that, according to published scientific reports, are up-regulated when bacteria are exposed to sound.  We will identify promoters that seem to be induced by pathways that respond to sound.  The selected promoters will be used to design new genetic devices that will generate chromogenic or fluorescent reporters when a cell is exposed to sounds.  We will use the new genetic devices to correlate sound frequencies, amplitudes, and exposure times with levels of gene expression.</p>
 
                                   <p><b>Impact</b><br> If we are successful in using sound to induce gene express in E. coli, the impact is unclear.  Our Human Practices team has started discussions with molecular biologists, brewmasters, and managers of water treatment systems to explore the potential positive and negative impacts of using sound to induce gene expression.  We will also have design thinking workshops focused on exploring the potential use and misuse of the technology.</p>
 
                                   <p><b>Impact</b><br> If we are successful in using sound to induce gene express in E. coli, the impact is unclear.  Our Human Practices team has started discussions with molecular biologists, brewmasters, and managers of water treatment systems to explore the potential positive and negative impacts of using sound to induce gene expression.  We will also have design thinking workshops focused on exploring the potential use and misuse of the technology.</p>
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Latest revision as of 03:46, 18 October 2018

Untitled-14

OVERVIEW

It is routine to trigger the production of a protein in engineered E. coli using a small molecule such as arabinose, lactose, and rhamnose. Our project is focused on answering the question, “Can sound be used to induce gene expression in engineered E. coli?” Prior iGEM projects demonstrated that sound can be used to activate eukaryotic cells. Review of the scientific literature revealed projects where exposing E. coli to sound correlated with more vigorous growth in cell cultures and increased production of proteins.

In 2008, the University of California, Berkeley (UC Berkeley) iGEM team contributed five genetic parts to the iGEM community. The genetic parts were derived from DNA sequences found upstream from genes that were potentially upregulated when the cells were exposed to sound. The UC Berkeley iGEM team was unable to demonstrate definitively that the genetic parts could be used as sound inducible promoters of gene expression. We decided to build on their work. We designed, built, and tested new genetic devices using the Berkeley promoters that were intended to detect sound and stimulate the expression of reporter proteins.

Members of the FSU iGEM team also investigated genes that were up-regulated when exposed to sound, according to other scientific literature. These signaling pathways and promoters were then analyzed and used to build new genetic devices which included reporter proteins that signal different levels of expression. Our new cells were tested at different sound frequencies and amplitudes to see which cells reported the highest level of reporter protein expression.

Audio induction has many possible impacts, the limit to which is still unclear. Our Human Practices team started a discussion in the fields of molecular biology, brewery, and law enforcement to explore the potential positive and negative impacts of using sound to induce gene expression.