Difference between revisions of "Team:US AFRL CarrollHS/Safety"

(Prototype team page)
 
 
(11 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{US_AFRL_CarrollHS}}
+
{{US_AFRL_CarrollHS/WikiStripDown}} {{US_AFRL_CarrollHS/Layout!}} {{US_AFRL_CarrollHS/Bootstrap1}} {{US_AFRL_CarrollHS/Bootstrap2}} {{US_AFRL_CarrollHS/Bootstrap3}} {{US_AFRL_CarrollHS/Bootstrap4}} {{US_AFRL_CarrollHS/Bootstrap8}} {{US_AFRL_CarrollHS/navbar}} {{US_AFRL_CarrollHS/hero}}  
 
<html>
 
<html>
  
 +
<div class="hero">
 +
      <img src="https://static.igem.org/mediawiki/2018/1/1e/T--US_AFRL_CarrollHS--SafetyHeader.jpg" alt="Safety" style="margin-top: 40px;">
 +
</div>
  
−
<div class="column full_size">
 
−
 
−
<h1> Safety </h1>
 
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
−
 
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
  
 +
<div class="background">
 +
<div class="row"><p>Both labs at the Air Force Research Laboratory in which we conducted research this summer were classified as Biosafety Level 2. The majority of the work was performed on open benches, with some occurring under BSL2 hoods. All bacteria chassis used over the summer (DH5 Alpha, <i>E. Coli</i> Nissle 1917, etc.) were considered nonpathogenic.</p>
 +
<p>Experiments and shipment of DNA (to the registry, for sequencing, etc.) were carried out safely and efficiently in accordance with the advice and guidelines of our mentors. Each student underwent Newcomer's Training that instructed them on administrative/office safety, emergency protocols (fire, tornado), how to use standard personal protective equipment (PPE) and emergency equipment (eye wash and shower), and chemical hygiene.</p>
 +
<p>Our labs were supervised by our mentors for day to day lab safety, but training and responding to specific questions were conducted by Tenika DeArmond for Lab 1 (RH), and Vanessa Varaljay and BJ Burke for Lab 2 (RX).</p>
 +
<p>Finally, our labs are routinely inspected and training is completed in accordance with regional safety guidelines. We follow federal (OSHA- 29 CFR 1910.1450, CFR 1910.132(F), CFR 1910.1030, CFR 1910.1096), Air Force Occupational Safety and Health (AFOSH), Wright Patterson Air Force Base Instruction, and lab specific standards.</p>
 
</div>
 
</div>
  
−
 
+
<div class="row"></div>
−
<div class="column two_thirds_size">
+
−
<h3>Safe Project Design</h3>
+
−
 
+
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
−
 
+
−
<ul>
+
−
<li>Choosing a non-pathogenic chassis</li>
+
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
−
 
+
 
</div>
 
</div>
  
−
<div class="column third_size">
 
−
<h3>Safe Lab Work</h3>
 
−
 
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
 
−
 
−
 
−
<h3>Safe Shipment</h3>
 
−
 
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
−
</div>
 
  
  
 
</html>
 
</html>
 +
{{US_AFRL_CarrollHS/footer}}

Latest revision as of 03:47, 18 October 2018

Safety

Both labs at the Air Force Research Laboratory in which we conducted research this summer were classified as Biosafety Level 2. The majority of the work was performed on open benches, with some occurring under BSL2 hoods. All bacteria chassis used over the summer (DH5 Alpha, E. Coli Nissle 1917, etc.) were considered nonpathogenic.

Experiments and shipment of DNA (to the registry, for sequencing, etc.) were carried out safely and efficiently in accordance with the advice and guidelines of our mentors. Each student underwent Newcomer's Training that instructed them on administrative/office safety, emergency protocols (fire, tornado), how to use standard personal protective equipment (PPE) and emergency equipment (eye wash and shower), and chemical hygiene.

Our labs were supervised by our mentors for day to day lab safety, but training and responding to specific questions were conducted by Tenika DeArmond for Lab 1 (RH), and Vanessa Varaljay and BJ Burke for Lab 2 (RX).

Finally, our labs are routinely inspected and training is completed in accordance with regional safety guidelines. We follow federal (OSHA- 29 CFR 1910.1450, CFR 1910.132(F), CFR 1910.1030, CFR 1910.1096), Air Force Occupational Safety and Health (AFOSH), Wright Patterson Air Force Base Instruction, and lab specific standards.

Contact us @iGEMLabPats or iGEM@carrollhs.org