Difference between revisions of "Team:US AFRL CarrollHS/Human Practices"

 
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      <img src="https://static.igem.org/mediawiki/2018/6/6e/T--US_AFRL_CarrollHS--HumanPracticesHeader.jpg" alt="Human Practices" style="margin-top: -7px">
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<div class="row"><h1>Outreach</h1></div>
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<div class="row"><p>As a team, we presented at many of our school events such as schedule pick up day, various club days, and parent-teacher conferences. We had a poster station and actively passed out flyers at these events to spread the word about our team. These events are an effective way to reach an audience that is interested in the work we are doing. These flyers are also a way to raise interest in next year's team so that we can continue the team in future years. We also reach parents this way, and we get to address many of their with the superstitions of genetically modified organisms. These types of open-ended conversation give the public a better understanding of the benefits of using our engineered microbe and synthetic biology in general.</p></div>
  
<div class="column full_size judges-will-not-evaluate">
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<h3>★  ALERT! </h3>
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  <div class="col-md-12 text-center"><img src="https://static.igem.org/mediawiki/2018/f/f5/T--US_AFRL_CarrollHS--PickupDay.jpeg" style="transform:rotate(90deg); width:70%; margin-top: 11%; margin-bottom: 9%;"></div></div>
<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<div class="row">
<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="col-sm-12 label"><p class="text-center">First year member Chris Guptil talking to a student at our high school about Synthetic Biology!</p></div>
 
</div>
 
</div>
  
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<div class="row"><h2>Advanced BioFuels USA</h2></div>
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<div class="row"><p>Along with educating students at our high school and local grade schools, we also made an effort to educate the public as a whole about synthetic biology and iGEM. Thanks to Advanced Biofuels USA, we were able to do this by publishing an article on their website. Additionally, we made a music video about synthetic biology and iGEM to further raise public awareness of synthetic biology. We posted this on our social media, and Advanced Biofuels USA also linked to the video on their website.</p></div>
  
<div class="clear"></div>
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<div class="row"><p><a href="https://advancedbiofuelsusa.info/eliminating-biofilms-in-biofuels-a-bacterial-approach">Advanced Biofuels Article</a></p></div>
  
 +
<div class="row"><p><a href="https://advancedbiofuelsusa.info/labpats-lose-yourself-video-especially-for-biologists-the-igem-edition">Music Video on Advanced Biofuels Website</a></p></div>
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<div class="row"><p>You can also watch the video on our <a href="https://2018.igem.org/Team:US_AFRL_CarrollHS">home page</a>.</p></div>
  
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<div class="column full_size">
 
  
<h1>Human Practices</h1>
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<img src="https://static.igem.org/mediawiki/2018/e/e1/T--US_AFRL_CarrollHS--_WhiteNavyDNA.png" style="width: 100%;">
<p>
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At iGEM we believe societal considerations should be upfront and integrated throughout the design and execution of synthetic biology projects. “Human Practices” refers to iGEM teams’ efforts to actively consider how the world affects their work and the work affects the world. Through your Human Practices activities, your team should demonstrate how you have thought carefully and creatively about whether your project is responsible and good for the world. We invite you to explore issues relating (but not limited) to the ethics, safety, security, and sustainability of your project, and to show how this exploration feeds back into your project purpose, design and execution.
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<div class="background2">
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<div class="row"><h1>Starting a new iGEM team</h1></div>
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<div class="row"><p>Virginia Tech contacted our team about trying to start a team of their own. We discussed what iGEM is, how to create a successful team, the difficulties we faced as a first year team, and how we overcame those issues last year. We also talked about Virginia Tech's reasons for starting an iGEM team, one of them being that their science department emphasizes the team aspect of science in their courses, making iGEM a natural fit for their school. Finally, we told them about our current project and our project from last year, and explained how working on those projects in the context of iGEM affected their final outcome.</p></div>
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</div>
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<img src="https://static.igem.org/mediawiki/2018/6/6a/T--US_AFRL_CarrollHS--_NavyWhiteDNA.png" style="width: 100%;">
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<div class="background">
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<div class="row"><h1>Input from Industry</h1></div>
 +
<div class="row"><p>
 +
Over the summer, the team traveled and spoke with various companies and experts who work with biofuels.  One such person is Scott Fenwick, who is the technical director of the National Biodiesel Board. He expressed concerns about having more microbiology in the fuel, and he gave us many big picture ideas about how he believed that the contamination of tanks would be eliminated within about ten years. In light of this concern, we decided to try to use our microbe to prevent biofilms from forming to keep concentrations both of the organisms in the fuel and of our engineered microbe as low as possible. This proposal did allay some of his fears, but he still emphasized his earlier points about adding more microbiology, and he said he would feel better about adding our microbe if there was some control over the growth of the engineered microbe.
 
</p>
 
</p>
  
 +
<p>
 +
The team also visited Beckett, a Cleveland based company that manufactures combustion burners. We spoke with them about how the push for biofuel is causing a number of problems because the current infrastructure is designed to accommodate traditional fuels, not biofuel. Because of this, many pipes, tanks, or other parts that come into contact with fuel will experience a loss in longevity or function when used with biofuels. Changing this infrastructure is one of the hurdles that would have to be overcome to implement biofuels. One of Beckett's main issues was the contamination of B20 or 20% biodiesel that accelerated the aging of many components they use in their burners. Our team discussed our solution with a Beckett representative, and also asked questions concerning what Beckett or any other company would want to see in a solution to biofuel contamination. Within our project, we spoke about trying to prevent microbial growth early on instead of trying to kill the mix of bacteria and fungi that make up the biofilm.</p>
  
 +
<p>
 +
Finally, the team traveled to a refining plant owned by Marathon, a national petroleum manufacturer, in Cincinnati, Ohio to speak about biofuels. The company in recent years has had an interest starting to manufacture biofuels. The team talked to a chemical engineer on site at Marathon about some of the problems the biofuel industry is facing with contamination. Interestingly, the staff at the plant conveyed that all of the contamination that was happening occurred during the transportation and storage of the fuel. This made us consider when our microbe should be implemented, and, after talking to the refining plant staff, we realized our engineered microbe would not need to be added before the refining process. </p>
  
<p>For more information, please see the <a href="https://2018.igem.org/Human_Practices">Human Practices Hub</a>. There you will find:</p>
 
 
<ul>
 
<li> an <a href="https://2018.igem.org/Human_Practices/Introduction">introduction</a> to Human Practices at iGEM </li>
 
<li>tips on <a href="https://2018.igem.org/Human_Practices/How_to_Succeed">how to succeed</a> including explanations of judging criteria and advice about how to conduct and document your Human Practices work</li>
 
<li>descriptions of <a href="https://2018.igem.org/Human_Practices/Examples">exemplary work</a> to inspire you</li>
 
<li>links to helpful <a href="https://2018.igem.org/Human_Practices/Resources">resources</a></li>
 
<li>And more! </li>
 
</ul>
 
 
  
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  <div class="col-md-6 text-center"><img src="https://static.igem.org/mediawiki/2018/7/7a/T--US_AFRL_CarrollHS--Marathon.png" width="100%"></div>
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<p>On this page, your team should document all of your Human Practices work and activities. You should write about the Human Practices topics you considered in your project, document any activities you conducted to explore these topics (such as engaging with experts and stakeholders), describe why you took a particular approach (including referencing any work you built upon), and explain if and how you integrated takeaways from your Human Practices work back into your project purpose, design and/or execution. </p>
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<div class="row">
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<div class="col-sm-6 label"><p class="text-center">The team visiting Beckett and learning about biofuel related problems in the industry</p></div>
<p>If your team has gone above and beyond in work related to safety, then you should document this work on your Safety wiki page and provide a description and link on this page. If your team has developed education and public engagement efforts that go beyond a focus on your particular project, and for which would like to nominate your team for the Best Education and Public Engagement Special Prize, you should document this work on your Education and Education wiki page and provide a description and link here. </p>
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<div class="col-sm-6 label"><p class="text-center">The team visiting the Cincinnati Refining Division, a subsidiary of Marathon that produces biofuels!</p></div>
 
</div>
 
</div>
 
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<p>The iGEM judges will review this page to assess whether you have met the Silver and/or Gold medal requirements based on the Integrated Human Practices criteria listed below. If you nominate your team for the <a href="https://2018.igem.org/Judging/Awards">Best Integrated Human Practices Special Prize</a> by filling out the corresponding field in the <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a>, the judges will also review this page to consider your team for that prize.
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<div class="col-sm-4 label"><p class="text-center">The team members and their mentor Chia discuss biofuels with the Beckett representative</p></div>
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<div class="col-sm-4 label"><p class="text-center">An experiment run by Beckett showing contamination of biofuels</p></div>
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<div class="col-sm-4 label"><p class="text-center">Team members Max and Hayley talking to Scott Fenwick, the technical director of the National Biodiesel Board, during a conference call</p></div>
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<div class="column full_size">
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<img src="https://static.igem.org/mediawiki/2018/e/e1/T--US_AFRL_CarrollHS--_WhiteNavyDNA.png" style="width: 100%;">
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<h3>Silver Medal Criterion #3</h3>
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<p>Convince the judges you have thought carefully and creatively about whether your work is responsible and good for the world. Document how you have investigated these issues and engaged with your relevant communities, why you chose this approach, and what you have learned. Please note that surveys will not fulfill this criteria unless you follow scientifically valid methods. </p>
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<div class="row"><h1>Air Force Research Laboratory</h1></div>
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<div class="row"><p>We also discussed our project with Dr. Naik, a chief scientist at the Air Force Research Laboratory (AFRL),  who provided us with feedback on our project and valuable background information. Dr. Wendy Goodson from AFRL was also able to supply us with isolates of fungi and bacteria from actual biofuel fuel tanks, which allowed us to design our microbe to target specifically the organisms found in fuel tanks. For example, we were able to use <i>Psuedomonas aeruginosa</i> as a target for our detect mechanism since we knew it was found in fuel tanks, and we were able to test our destroy mechanism on one of the fungal isolates, <i>Yarrowia lipolytica</i>. Additionally, Dr. Goodson also supplied us with a number of pictures of biofilms, fuel tanks, and pipes, along with a scanning electron microscope image of a biofilm, all of which helped us envision the problem and develop our solution. </p></div>
  
<h3>Gold Medal Criterion #1</h3>
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<div class="row">
<p>Expand on your silver medal activity by demonstrating how you have integrated the investigated issues into the purpose, design and/or execution of your project. Document how your project has changed based upon your human practices work.
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  <div class="col-md-6 text-center"><img src="https://static.igem.org/mediawiki/2018/7/75/T--US_AFRL_CarrollHS--NaikPic.jpg" style="width:90%;"></div>
</p>
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  <div class="col-md-6 text-center"><img src="https://static.igem.org/mediawiki/2018/4/43/T--US_AFRL_CarrollHS--WendyGoodson.jpeg" style="width:90%;"></div>
 
</div>
 
</div>
  
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<div class="row">
  
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<div class="col-sm-6 label"><p class="text-center">The team and mentors with Dr. Naik (first row, far left) after presenting our project!</p>
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</div>
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<div class="col-sm-6 label">
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<p class="text-center">(From the left) Team members Annie, Max, Jonah, and Yazmin with Dr. Wendy Goodson (middle)!</p>
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</div>
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</div>
  
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<img src="https://static.igem.org/mediawiki/2018/6/6a/T--US_AFRL_CarrollHS--_NavyWhiteDNA.png" style="width: 100%;">
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<div class="row"><h1>Meeting with Mike Turner</h1></div>
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<div class="row"><p>
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We were able to reach out and contact Mike Turner, the U.S. Representative for Ohio's 10th congressional district and has served that position since 2003. We were very fortunate to be able to talk to Mike Turner about our project and discuss possible legislature idea based on our research and completed project. Throughout our research, we were surprised to see how little legislature was in place and how slow the change to biofuel was because of various factors such as fouling. One of our goals established at the beginning of the project was to talk to local and state legislature in order to display our work and bring awareness to biofuels. The schedule of the day is shown below. </p>
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  <div class="col-md-12 text-center"><img src="https://static.igem.org/mediawiki/2018/9/91/T--US_AFRL_CarrollHS--_MikeTurnerSchedule2.jpeg"  style="width:70%;"></div></div>
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 +
<div class="col-sm-12 label"><p class="text-center">An agenda for our planned meeting with Mike Turner</p></div>
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<div class="row">
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<p>During the meeting, we hope to give a brief presentation of our project We plan to ask him questions regarding if he knew of the current problems with biofuels, and of Ohio's or the nation's storage of biofuels. Another main question we had in mind was to ask him whether he would be willing to help create jobs in Ohio based on synthetic biology research. Check back after the Jamboree for our results and pictures!
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</p></div>
  
<div class="column full_size">
 
<h3>Best Integrated Human Practices Special Prize</h3>
 
  
<p>To compete for the Best Integrated Human Practices prize, please describe your work on this page and also fill out the description on the judging form. </p>
 
  
<p>How does your project affect society and how does society influence the direction of your project? How might ethical considerations and stakeholder input guide your project purpose and design and the experiments you conduct in the lab? How does this feedback enter into the process of your work all through the iGEM competition? Document a thoughtful and creative approach to exploring these questions and how your project evolved in the process to compete for this award!</p>
 
<p>You must also delete the message box on the top of this page to be eligible for this prize.</p>
 
  
 
</div>
 
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Latest revision as of 03:47, 18 October 2018


Human Practices

Outreach

As a team, we presented at many of our school events such as schedule pick up day, various club days, and parent-teacher conferences. We had a poster station and actively passed out flyers at these events to spread the word about our team. These events are an effective way to reach an audience that is interested in the work we are doing. These flyers are also a way to raise interest in next year's team so that we can continue the team in future years. We also reach parents this way, and we get to address many of their with the superstitions of genetically modified organisms. These types of open-ended conversation give the public a better understanding of the benefits of using our engineered microbe and synthetic biology in general.

First year member Chris Guptil talking to a student at our high school about Synthetic Biology!

Advanced BioFuels USA

Along with educating students at our high school and local grade schools, we also made an effort to educate the public as a whole about synthetic biology and iGEM. Thanks to Advanced Biofuels USA, we were able to do this by publishing an article on their website. Additionally, we made a music video about synthetic biology and iGEM to further raise public awareness of synthetic biology. We posted this on our social media, and Advanced Biofuels USA also linked to the video on their website.

You can also watch the video on our home page.

Starting a new iGEM team

Virginia Tech contacted our team about trying to start a team of their own. We discussed what iGEM is, how to create a successful team, the difficulties we faced as a first year team, and how we overcame those issues last year. We also talked about Virginia Tech's reasons for starting an iGEM team, one of them being that their science department emphasizes the team aspect of science in their courses, making iGEM a natural fit for their school. Finally, we told them about our current project and our project from last year, and explained how working on those projects in the context of iGEM affected their final outcome.

Input from Industry

Over the summer, the team traveled and spoke with various companies and experts who work with biofuels. One such person is Scott Fenwick, who is the technical director of the National Biodiesel Board. He expressed concerns about having more microbiology in the fuel, and he gave us many big picture ideas about how he believed that the contamination of tanks would be eliminated within about ten years. In light of this concern, we decided to try to use our microbe to prevent biofilms from forming to keep concentrations both of the organisms in the fuel and of our engineered microbe as low as possible. This proposal did allay some of his fears, but he still emphasized his earlier points about adding more microbiology, and he said he would feel better about adding our microbe if there was some control over the growth of the engineered microbe.

The team also visited Beckett, a Cleveland based company that manufactures combustion burners. We spoke with them about how the push for biofuel is causing a number of problems because the current infrastructure is designed to accommodate traditional fuels, not biofuel. Because of this, many pipes, tanks, or other parts that come into contact with fuel will experience a loss in longevity or function when used with biofuels. Changing this infrastructure is one of the hurdles that would have to be overcome to implement biofuels. One of Beckett's main issues was the contamination of B20 or 20% biodiesel that accelerated the aging of many components they use in their burners. Our team discussed our solution with a Beckett representative, and also asked questions concerning what Beckett or any other company would want to see in a solution to biofuel contamination. Within our project, we spoke about trying to prevent microbial growth early on instead of trying to kill the mix of bacteria and fungi that make up the biofilm.

Finally, the team traveled to a refining plant owned by Marathon, a national petroleum manufacturer, in Cincinnati, Ohio to speak about biofuels. The company in recent years has had an interest starting to manufacture biofuels. The team talked to a chemical engineer on site at Marathon about some of the problems the biofuel industry is facing with contamination. Interestingly, the staff at the plant conveyed that all of the contamination that was happening occurred during the transportation and storage of the fuel. This made us consider when our microbe should be implemented, and, after talking to the refining plant staff, we realized our engineered microbe would not need to be added before the refining process.


The team visiting Beckett and learning about biofuel related problems in the industry

The team visiting the Cincinnati Refining Division, a subsidiary of Marathon that produces biofuels!

The team members and their mentor Chia discuss biofuels with the Beckett representative

An experiment run by Beckett showing contamination of biofuels

Team members Max and Hayley talking to Scott Fenwick, the technical director of the National Biodiesel Board, during a conference call

Air Force Research Laboratory

We also discussed our project with Dr. Naik, a chief scientist at the Air Force Research Laboratory (AFRL), who provided us with feedback on our project and valuable background information. Dr. Wendy Goodson from AFRL was also able to supply us with isolates of fungi and bacteria from actual biofuel fuel tanks, which allowed us to design our microbe to target specifically the organisms found in fuel tanks. For example, we were able to use Psuedomonas aeruginosa as a target for our detect mechanism since we knew it was found in fuel tanks, and we were able to test our destroy mechanism on one of the fungal isolates, Yarrowia lipolytica. Additionally, Dr. Goodson also supplied us with a number of pictures of biofilms, fuel tanks, and pipes, along with a scanning electron microscope image of a biofilm, all of which helped us envision the problem and develop our solution.

The team and mentors with Dr. Naik (first row, far left) after presenting our project!

(From the left) Team members Annie, Max, Jonah, and Yazmin with Dr. Wendy Goodson (middle)!

Meeting with Mike Turner

We were able to reach out and contact Mike Turner, the U.S. Representative for Ohio's 10th congressional district and has served that position since 2003. We were very fortunate to be able to talk to Mike Turner about our project and discuss possible legislature idea based on our research and completed project. Throughout our research, we were surprised to see how little legislature was in place and how slow the change to biofuel was because of various factors such as fouling. One of our goals established at the beginning of the project was to talk to local and state legislature in order to display our work and bring awareness to biofuels. The schedule of the day is shown below.

An agenda for our planned meeting with Mike Turner

During the meeting, we hope to give a brief presentation of our project We plan to ask him questions regarding if he knew of the current problems with biofuels, and of Ohio's or the nation's storage of biofuels. Another main question we had in mind was to ask him whether he would be willing to help create jobs in Ohio based on synthetic biology research. Check back after the Jamboree for our results and pictures!