Difference between revisions of "Team:NEU China B/Notebook"

 
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{{NEU_China_B}}
 
{{NEU_China_B}}
 
<html>
 
<html>
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<div class="center-block" style="max-width:800px;">
 
<h1>
 
<h1>
−
    Notebook
+
Notebook
−
</h1>
+
</h1>
−
<h2>
+
<h2>
−
    2018.1
+
2018.1
−
</h2>
+
</h2>
−
<p>
+
<div class="p">
−
    Team construction, ensure the duty in lab, take a test about security of lab, start to take regular weekly meeting.
+
Team construction, ensure the duties in lab, take a test about security in lab, start to take regular weekly meetings.
−
</p>
+
</div>
−
<h2>
+
<h2>
−
    2018.2
+
2018.2
−
</h2>
+
</h2>
−
<p>
+
<div class="p">
−
    We tried to be familiar with the rules of IGEM. In addition, according to the previous project, we discussed our
+
We tried to be familiar with the rules of IGEM. In addition, according to the previous project, we discussed our own project and we decided to plan the project roughly.
−
    own project and we decided to plan the project roughly.
+
</div>
−
</p>
+
<h2>
−
<h2>
+
2018.3
−
    2018.3
+
</h2>
−
</h2>
+
<div class="p">
−
<p>
+
We detailed the plan of our experiment. We choose E. coli K-12 strain, plasmid pET28a and pCDFDuet-1.
−
    We detailed the plan of our experiment. We choose e.coli K-12, plasmid pET28a and pCDFDuet-1.
+
</div>
−
</p>
+
<h2>
−
<h2>
+
2018.4
−
    2018.4
+
</h2>
−
</h2>
+
<div class="p">
−
<p>
+
Bought materials, investigation, Genomic extraction
−
    Bought materials, investigation, Genomic extraction
+
</div>
−
</p>
+
<h2>
−
<h2>
+
2018.5
−
    2018.5
+
</h2>
−
</h2>
+
<div class="p">
−
<p>
+
We tried to extract our genome and PCR the first gene----LsrACDBFG( 6x)
−
    We tried to extract our genome and pcr the first gene----LsrACDBFG
+
</div>
−
</p>
+
<h2>
−
<h2>
+
2018.6
−
    2018.6
+
</h2>
−
</h2>
+
<h3>
−
<h3>
+
1st week(6/1/2018-6/7/2018)
−
    1st week(6/1/2018-6/7/2018)
+
</h3>
−
</h3>
+
<div class="p">
−
<p>
+
Extracted genome of E. coli.  
−
    Extracted genome of e.coli.
+
<br> Designed new primer.  
−
    <br> Designed new primer.
+
<br> PCR fragment LsrACDBFG with minimal and maximum system respectively.
−
    <br> PCR fragment LSRACDBFG with mm and Max system respectively.
+
</div>
−
</p>
+
<h3>
−
<h3>
+
2nd week(6/8/2018-6/14/2018)
−
    2nd week(6/8/2018-6/14/2018)
+
</h3>
−
</h3>
+
<div class="p">
−
<p>
+
<br>PCR  target gene LsrACDBFG in three parts  
−
    <br>Pcr target gene LsrACDBFG in three parts
+
<br>Human practice  
−
    <br>Human practice
+
                                                                        <br>Enzyme-cut plasmid pCDFDuet-1 and target fragment GFP  
−
    <br>Enzyme-cut plasmid PCDF and target fragment GFP
+
                                                                        <br>Constructed GFP expression Vectors
−
    <br>Constructed GFP expression Vectors
+
−
</p>
+
−
<h3>
+
−
    3rd week(6/15/2018-6/21/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>PCR fragment lsrACDBFG with mm system
+
−
    <br>Pcr target fragment lsrACDBFG and plasmid pet-28(a)
+
−
    <br>Enzyme-cut target sequence.
+
−
    <br>Constructed 6x plasmid
+
−
    <br>Transformed 6x plasmid vectors into pet-28 (a) competent cells
+
−
    <br>Extracted 6x plasmid, enzyme-cut identification to verify the effect of the build
+
−
    <br>Sequencing
+
−
    <br>Detection of recombinant strain 6x gene protein expression
+
−
</p>
+
−
<h3>
+
−
    4th week(6/22/2018-6/28/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>Pcr target gene LsrACDBFG in three parts
+
−
    <br>Connecting 6x-1, 6x-2, 6x-3
+
−
    <br>Purified fragments
+
−
    <br>Constructed 6x plasmid
+
−
    <br>Transformed 6x plasmid vectors into pet-28 (a) competent cells
+
−
    <br>Extracting 6x plasmid from recombinant strains
+
−
    <br>Enzyme-cut identification to detect build effects
+
−
</p>
+
  
−
<h2>
+
</div>
−
    2018.7
+
<h3>
−
</h2>
+
3rd week(6/15/2018-6/21/2018)
−
<h3>
+
</h3>
−
    1st week(7/1/2018)
+
<div class="p">
−
</h3>
+
<br>PCR fragment LsrACDBFG with mm system
−
<p>
+
<br>PCR target fragment LsrACDBFG and plasmid pet-28(a)+
−
    <br>Pcr target gene GFP
+
<br>Enzyme-cut target sequence.
−
    <br>Constructed plasmid GFP
+
<br>Constructed 6x plasmid
−
</p>
+
<br>Transformed 6x plasmid vectors into pet-28 (a)+ competent cells
−
<h3>
+
<br>Extracted 6x plasmid, enzyme-cut identification to verify the effect of the build
−
    2nd week
+
<br>Sequencing
−
</h3>
+
<br>Detection of recombinant strain 6x gene protein expression
−
<p>
+
−
    <br>Pcr target gene LsrACDBFG in three parts
+
−
    <br>Pcr target gene GFP
+
−
</p>
+
  
−
<h3>
+
</div>
−
    3rd week
+
<h3>
−
</h3>
+
4th week(6/22/2018-6/28/2018)
−
<p>
+
</h3>
−
    <br>Connected lsrA and GFP
+
<div class="p">
−
    <br>Constructed plasmid 6x + GFP
+
<br>PCR target gene LsrACDBFG in three parts
−
    <br>Transformed 6x plasmid vectors into pet-28 (a) competent cells
+
<br>Connecting 6x-1, 6x-2, 6x-3 ( 3 repeated tubes)
−
    <br>Extracting 6x + GFP plasmid from recombinant strains
+
<br>Purified fragments
−
    <br>Enzyme-cut identification to detect build effects
+
<br>Constructed 6x plasmid
 +
<br>Transformed 6x plasmid vectors into pet-28 (a)+ competent cells  
 +
<br>Extracting 6x plasmid from recombinant strains  
 +
<br>Enzyme-cut identification to detect build effects
  
−
</p>
+
</div>
−
<h3>
+
−
    4th week(7/22/2018-7/28/2018)
+
<h2>
−
</h3>
+
2018.7
−
<p>
+
</h2>
−
    <br>Pcr plasmid strain 6x + GFP, proved that we construct plasmid 6x + GFP successfully
+
<h3>
−
    <br>Detection of recombinant strains 6X+GFO gene expression protein
+
1st week(7/1/2018)
−
</p>
+
</h3>
−
<h2>
+
<div class="p">
−
    2018.8
+
<br>PCR target gene GFP  
−
</h2>
+
<br>Constructed plasmid GFP
−
<h3>
+
−
    1st week(8/1/2018-8/7/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>Extracted plasmid pet-28(a)
+
−
    <br>Pcr target fragment Luxs
+
−
</p>
+
−
<h3>
+
−
    2nd (8/8/2018-8/14/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>Extracted total DNA from Escherichia coli
+
−
    <br>Lldr and plasmid PCDF of enzyme-cut target fragments
+
−
    <br>Constructed plasmid Lldr
+
−
</p>
+
−
<h3>
+
−
    3rd (8/15/2018-8/21/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>Pcr target fragment Luxs
+
−
    <br>Constructed plasmid O+Luxs+Lldr
+
−
    <br>Transformed O+Luxs+Lldr plasmid vectors into DH5-aα competent cells
+
−
    <br>Extraction of O+LUXS+LLDR plasmid, enzyme-cut identification to verify the effect of construction
+
−
    <br>Sequencing
+
−
    <br>Detection of recombinant strains of protein in the target gene expression
+
−
</p>
+
−
<h3>
+
−
    4th (8/22/2018-8/28/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>Pcr target fragment LsrA, GFP
+
−
    <br>Connected LsrA and GFP
+
−
    <br>Constructed plasmid LsrA+GFP
+
−
    <br>Pcr target fragment Op
+
−
    <br>Connected Op and GFP
+
−
    <br>Constructed plasmid Op+GFP
+
−
    <br>Constructed plasmid 6x+GFP
+
−
</p>
+
−
<h2>
+
−
    2018.9
+
−
</h2>
+
−
<h3>
+
−
    (9/1/2018-9/15/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>Transformed LsrA+GFP, Op+GFP, 6x+GFP plasmid vectors into DH5-aα competent cells
+
−
    <br>Extraction of LSRA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid from recombinant strains
+
−
    <br>Enzyme-cut identification of LSRA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid to verify the effect of
+
−
    construction
+
−
    <br>Sequencing
+
−
    <br>We combined the various plasmids to confirm the function of the whole system.
+
−
    <br>Detection of recombinant strain protein expression, verifying the function of constructed parts in recombinant
+
−
    strains
+
−
</p>
+
−
<h3>
+
−
    (9/16/2018-9/30/2018)
+
−
</h3>
+
−
<p>
+
−
    <br>We combined the various plasmids to confirm the function of the whole system
+
−
    <br>Refined and optimized the project parts
+
−
</p>
+
  
−
<h3>
+
</div>
−
    2018.10
+
<h3>
−
</h3>
+
2nd week
−
<p>
+
</h3>
−
    Sent BioBricks, dedicated to the paperwork of the wiki.
+
<div class="p">
−
</p>
+
<br>PCR target gene LsrACDBFG in three pieces
 +
<br>PCR target gene GFP
  
 +
</div>
 +
 +
<h3>
 +
3rd week
 +
</h3>
 +
<div class="p">
 +
<br>Connected LsrA and GFP
 +
<br>Constructed plasmid 6x + GFP
 +
<br>Transformed 6x plasmid vectors into pet-28 (a)+ competent cells
 +
<br>Extracting 6x + GFP plasmid from recombinant strains
 +
<br>Enzyme-cut identification to detect build effects
  
−
</html>
+
 +
</div>
 +
<h3>
 +
4th week(7/22/2018-7/28/2018)
 +
</h3>
 +
<div class="p">
 +
<br>PCR plasmid strain 6x + GFP, proved that we construct plasmid 6x + GFP successfully
 +
<br>Detection of recombinant strains 6X+GFP gene expression protein
 +
 
 +
</div>
 +
<h2>
 +
2018.8
 +
</h2>
 +
<h3>
 +
1st week(8/1/2018-8/7/2018)
 +
</h3>
 +
<div class="p">
 +
<br>Extracted plasmid pet-28(a)+
 +
<br>PCR target fragment Luxs
 +
 
 +
</div>
 +
<h3>
 +
2nd (8/8/2018-8/14/2018)
 +
</h3>
 +
<div class="p">
 +
<br>Extracted total DNA from Escherichia coli
 +
<br>lldR and plasmid pCDFDuet-1 of enzyme-cut target fragments
 +
<br>Constructed plasmid lldR
 +
 
 +
</div>
 +
<h3>
 +
3rd (8/15/2018-8/21/2018)
 +
</h3>
 +
<div class="p">
 +
<br>PCr target fragment LuxS
 +
<br>Constructed plasmid O+LuxS+lldR
 +
<br>Transformed O+LuxS+lldR sequence into DH5-α competent cells
 +
<br>Extraction of O+LuxS+lldR plasmid, enzyme-cut identification to verify the effect of construction
 +
<br>Sequencing
 +
<br>Detection of recombinant strains of protein in the target gene expression
 +
 
 +
</div>
 +
<h3>
 +
4th (8/22/2018-8/28/2018)
 +
</h3>
 +
<div class="p">
 +
<br>PCR target fragment LsrA, GFP
 +
<br>Connected LsrA and GFP
 +
<br>Constructed plasmid LsrA+GFP
 +
<br>PCR target fragment Op
 +
<br>Connected Op and GFP
 +
<br>Constructed plasmid Op+GFP
 +
<br>Constructed plasmid 6x+GFP
 +
 
 +
</div>
 +
<h2>
 +
2018.9
 +
</h2>
 +
<h3>
 +
(9/1/2018-9/15/2018)
 +
</h3>
 +
<div class="p">
 +
<br>Transformed LsrA+GFP, Op+GFP, 6x+GFP plasmid vectors into DH5-α competent cells
 +
<br>Extraction of LsrA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid from recombinant strains
 +
<br>Enzyme-cut identification of LsrA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid to verify the effect of construction
 +
<br>Sequencing
 +
<br>We combined the various plasmids to confirm the function of the whole system.
 +
<br>Detection of recombinant strain protein expression, verifying the function of constructed parts in recombinant strains
 +
 
 +
</div>
 +
<h3>
 +
(9/16/2018-9/30/2018)
 +
</h3>
 +
<div class="p">
 +
<br>We combined the various plasmids to confirm the function of the whole system
 +
<br>Refined and optimized the project parts
 +
 
 +
</div>
 +
 +
<h3>
 +
2018.10
 +
</h3>
 +
<div class="p">
 +
<br>Sent BioBricks, dedicated to the paperwork of the wiki.
 +
</div>
 +
 +
 +
</html>

Latest revision as of 03:49, 18 October 2018

Ruby - Responsive Corporate Tempalte

Notebook

2018.1

Team construction, ensure the duties in lab, take a test about security in lab, start to take regular weekly meetings.

2018.2

We tried to be familiar with the rules of IGEM. In addition, according to the previous project, we discussed our own project and we decided to plan the project roughly.

2018.3

We detailed the plan of our experiment. We choose E. coli K-12 strain, plasmid pET28a and pCDFDuet-1.

2018.4

Bought materials, investigation, Genomic extraction

2018.5

We tried to extract our genome and PCR the first gene----LsrACDBFG( 6x)

2018.6

1st week(6/1/2018-6/7/2018)

Extracted genome of E. coli.
Designed new primer.
PCR fragment LsrACDBFG with minimal and maximum system respectively.

2nd week(6/8/2018-6/14/2018)


PCR target gene LsrACDBFG in three parts
Human practice
Enzyme-cut plasmid pCDFDuet-1 and target fragment GFP
Constructed GFP expression Vectors

3rd week(6/15/2018-6/21/2018)


PCR fragment LsrACDBFG with mm system
PCR target fragment LsrACDBFG and plasmid pet-28(a)+
Enzyme-cut target sequence.
Constructed 6x plasmid
Transformed 6x plasmid vectors into pet-28 (a)+ competent cells
Extracted 6x plasmid, enzyme-cut identification to verify the effect of the build
Sequencing
Detection of recombinant strain 6x gene protein expression

4th week(6/22/2018-6/28/2018)


PCR target gene LsrACDBFG in three parts
Connecting 6x-1, 6x-2, 6x-3 ( 3 repeated tubes)
Purified fragments
Constructed 6x plasmid
Transformed 6x plasmid vectors into pet-28 (a)+ competent cells
Extracting 6x plasmid from recombinant strains
Enzyme-cut identification to detect build effects

2018.7

1st week(7/1/2018)


PCR target gene GFP
Constructed plasmid GFP

2nd week


PCR target gene LsrACDBFG in three pieces
PCR target gene GFP

3rd week


Connected LsrA and GFP
Constructed plasmid 6x + GFP
Transformed 6x plasmid vectors into pet-28 (a)+ competent cells
Extracting 6x + GFP plasmid from recombinant strains
Enzyme-cut identification to detect build effects

4th week(7/22/2018-7/28/2018)


PCR plasmid strain 6x + GFP, proved that we construct plasmid 6x + GFP successfully
Detection of recombinant strains 6X+GFP gene expression protein

2018.8

1st week(8/1/2018-8/7/2018)


Extracted plasmid pet-28(a)+
PCR target fragment Luxs

2nd (8/8/2018-8/14/2018)


Extracted total DNA from Escherichia coli
lldR and plasmid pCDFDuet-1 of enzyme-cut target fragments
Constructed plasmid lldR

3rd (8/15/2018-8/21/2018)


PCr target fragment LuxS
Constructed plasmid O+LuxS+lldR
Transformed O+LuxS+lldR sequence into DH5-α competent cells
Extraction of O+LuxS+lldR plasmid, enzyme-cut identification to verify the effect of construction
Sequencing
Detection of recombinant strains of protein in the target gene expression

4th (8/22/2018-8/28/2018)


PCR target fragment LsrA, GFP
Connected LsrA and GFP
Constructed plasmid LsrA+GFP
PCR target fragment Op
Connected Op and GFP
Constructed plasmid Op+GFP
Constructed plasmid 6x+GFP

2018.9

(9/1/2018-9/15/2018)


Transformed LsrA+GFP, Op+GFP, 6x+GFP plasmid vectors into DH5-α competent cells
Extraction of LsrA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid from recombinant strains
Enzyme-cut identification of LsrA+GFP plasmid, OP+GFP plasmid and 6X+GFP plasmid to verify the effect of construction
Sequencing
We combined the various plasmids to confirm the function of the whole system.
Detection of recombinant strain protein expression, verifying the function of constructed parts in recombinant strains

(9/16/2018-9/30/2018)


We combined the various plasmids to confirm the function of the whole system
Refined and optimized the project parts

2018.10


Sent BioBricks, dedicated to the paperwork of the wiki.