Difference between revisions of "Team:Lethbridge HS/Improve"

 
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background-image: url("https://static.igem.org/mediawiki/2018/0/00/T--Lethbridge_HS--parts2.png");text-align:center;
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background-image: url("https://static.igem.org/mediawiki/2018/6/6a/T--Lethbridge_HS--improve3.png");text-align:center;
 
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<div class="container-fluid;" style="margin-left:10%; margin-right:10%;">
 
<div class="container-fluid;" style="margin-left:10%; margin-right:10%;">
 
<br><br>
 
<br><br>
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<h1  style="font-size: 3vw; font-family:Montserrat;"class="w100" ><b>IMPROVED PART</b></h1>
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<p style="font-size: 40px; font-family: 'Open Sans'"> Cup 1</p>
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<center><img class="img-fluid"style="float:right; margin-left:10px;margin-bottom:5px;
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    width:  400px; height: ;" src="https://static.igem.org/mediawiki/2018/e/e9/T--Lethbridge_HS--Cup1.png"></center>
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<p style="font-size: 18px; font-family: 'Open Sans'"> <b>Original Part:</b><a href= "http://parts.igem.org/Part:BBa_K586000"> BBa_K586000 </a> </p>
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<p style="font-size: 18px; font-family: 'Open Sans'"><b>Submitted Part:</b> GreenfieldINUSA 2011</p>
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<p style="font-size: 18px; font-family: 'Open Sans'"><b>Designed by: </b>Chris Thompson</p>
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<p style="font-size: 18px; font-family: 'Open Sans'"><b>Rationale behind improvement:</b> We have improved this part by introducing a hexahistidine tag to it, this has proven to be the optimal length for purification. Adding the histidine tag creates the opportunity to use a simpler method of purification, as well as increase selectivity for the desired protein.</p>
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<p><h1></h1> <b></b>
 
<p><h1></h1> <b></b>
 
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Latest revision as of 03:51, 18 October 2018



IMPROVED PART

Cup 1

Original Part: BBa_K586000

Submitted Part: GreenfieldINUSA 2011

Designed by: Chris Thompson

Rationale behind improvement: We have improved this part by introducing a hexahistidine tag to it, this has proven to be the optimal length for purification. Adding the histidine tag creates the opportunity to use a simpler method of purification, as well as increase selectivity for the desired protein.