Difference between revisions of "Team:Jilin China"

 
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{{:Team:Jilin_China/CSS}}
 
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{{:Team:Jilin_China/CSS_Details}}
 
 
{{:Team:Jilin_China/Navbar}}
 
{{:Team:Jilin_China/Navbar}}
 
{{:Team:Jilin_China/Js}}
 
{{:Team:Jilin_China/Js}}
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<html><!--For CSS and Js-->
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{{:Team:Jilin_China/Home_CSS}}
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<section class=“s1” style=“height:50vh;”></s1>
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{{:Team:Jilin_China/Footer_CSS}}
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<h1>Home page is under construction. To view what we have already done, try navigation bar on the top.<h1>
+
<html>
 +
<style>
 +
 
 +
 
 +
</style>
 
</html>
 
</html>
 +
 +
<html>
 +
<div class="s1" style="display:none;height:99999%;"></div>
 +
<section class="ss1 home_banner">
 +
  <div class="banner_logo"></div>
 +
  <h1><span>The Collection of</span><br><span>SynRT</span><br><span>with Different Sensing Temperatures</span></h1>
 +
  <div class="bg-giraffe">
 +
  <div>
 +
    <div></div>
 +
    <div></div>
 +
    <div></div>
 +
    <div></div>
 +
  </div>
 +
  </div>
 +
</section>
 +
<section class="ss2 normal abstract">
 +
  <div>
 +
  <h1>ABSTRACT</h1>
 +
  <p>Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'-
 +
untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.</p>
 +
  <p>However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their application. To address this issue, we have created a synthetic RNA-based thermosensor (SynRT) toolkit with different sensing temperatures. Additionally, we built a matched search engine for users.
 +
  <p>The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:</p>
 +
  </div>
 +
</section>
 +
<section class="ss3 normal home_design">
 +
  <div class="bacteria">
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 +
  <center><p><b>Heat-inducible RNA-based Thermosensor</b></p></center>
 +
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  <center><p><b>Heat-repressible RNA-based Thermosensor</b></p></center>
 +
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 +
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 +
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 +
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 +
    </g>
 +
  </g>
 +
</svg>
 +
  <center><p><b>Cold-inducible RNA-based Thermosensor</b></p></center>
 +
 +
 +
  <script type="text/javascript">
 +
    $(document).ready(function(){
 +
      $("#checked_3").click(function(){
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    });
 +
  </script>
 +
  </div>
 +
  <div class="stem-loop cold-repressive">
 +
   
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  <input id="checked_4" type="checkbox" class="switch" />
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  <svg class="svg_animate" xmlns="http://www.w3.org/2000/svg" viewBox="0 0 599.75 278.7">
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    <defs>
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    <style>
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    fill: none;
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    stroke-width: 11px;
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    fill: #900;
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 +
    </style>
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    <title>Stroke Version_1</title>
 +
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 +
    <g class="hydrogen-bond">
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    <g id="text">
 +
      <text class="d" transform="translate(429.01 185.45)"></text>
 +
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 +
 +
    </g>
 +
 +
    <text id="ribosome_text" class="h" transform="translate(429.01 165.45)">RNAse III</text>
 +
 +
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 +
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 +
    </g> 
 +
<g id="RNAse_3">
 +
      <path class="g" d="M405.71,98.59h-51a12,12,0,0,0-12,12V176.7L381.85,188l-39.14,11.29v16.31a12,12,0,0,0,12,12h51a12,12,0,0,0,12-12v-105A12,12,0,0,0,405.71,98.59Z"/>
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 +
  </g>
 +
  </svg>
 +
  <center><p><b>Cold-repressible RNA-based Thermosensor</b></p></center>
 +
 +
 +
  <script type="text/javascript">
 +
    $(document).ready(function(){
 +
      $("#checked_4").click(function(){
 +
        if($(this).is(':checked')){
 +
          $(".cold-repressive").addClass("svg_checked");
 +
        }else{
 +
          $(".cold-repressive").removeClass("svg_checked");
 +
      }
 +
      });
 +
    });
 +
  </script>
 +
  </div>
 +
    <center><a href="https://2018.igem.org/Team:Jilin_China/Design">Learn More ></a></center>
 +
  </div>
 +
<div style="clear:both"></div>
 +
</section>
 +
<section class="ss3 normal hp">
 +
  <h1>Human Practices</h1>
 +
  <div>
 +
    <p>Several Exploration Improved</p>
 +
    <p>OUR TOOLKITs</p>
 +
    <p>Meanwhile Inspiring Us to Make</p>
 +
    <p>A DECADE <br>WORKFLOW</p>
 +
  </div>
 +
  <img src="https://static.igem.org/mediawiki/2018/e/e1/T--Jilin_China--Home--HP--1.svg" />
 +
    <a href="https://2018.igem.org/Team:Jilin_China/Human_Practices">Hear Our Stories</a>
 +
  <br clear="all"/>
 +
  <div>
 +
    <p>Several Innovative Kits</p>
 +
    <p>Promoting Us to</p>
 +
    <p>SPREAD and DISCUSS</p>
 +
    <p>Synthetic Biology</p>
 +
  </div>
 +
  <img src="https://static.igem.org/mediawiki/2018/b/bc/T--Jilin_China--Home--HP--2.svg"/>
 +
  <a href="https://2018.igem.org/Team:Jilin_China/Public_Engagement">Hear Our Stories</a>
 +
  <br clear="all"/>
 +
</section>
 +
<section class="ss4 normal model">
 +
  <h1>Model</h1><img src="https://static.igem.org/mediawiki/2018/b/b2/T--Jilin_China--Home_Model-curve.png" width="100%"></img>
 +
  <a href="https://2018.igem.org/Team:Jilin_China/Model">Learn More > </a>
 +
</section>
 +
<section class="ss5 normal">
 +
  <h1>More</h1>
 +
  <div>
 +
    <div>
 +
    <div class="ss5_background">
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    <a href="https://2018.igem.org/Team:Jilin_China/Background"><h3>Background</h3></a>
 +
    </div>
 +
    <div class="ss5_project">
 +
    <a href="https://2018.igem.org/Team:Jilin_China/Project/Overview"><h3>Project</h3></a>
 +
    </div>
 +
    </div>
 +
    <div>
 +
    <div class="ss5_result">
 +
    <a href="https://2018.igem.org/Team:Jilin_China/Result/Version_1"><h3>Results</h3></a>
 +
    </div>
 +
    <div class="ss5_engine">
 +
    <a href="https://2018.igem.org/Team:Jilin_China/Part/Search_Engine"><h3>Explorer</h3></a>
 +
    </div>
 +
    </div>
 +
    <div>
 +
    <div class="ss5_part">
 +
    <a href="https://2018.igem.org/Team:Jilin_China/Parts"><h3>Parts</h3></a>
 +
    </div>
 +
    <div class="ss5_team">
 +
    <a href="https://2018.igem.org/Team:Jilin_China/Team"><h3>Team</h3></a>
 +
    </div>
 +
    </div>
 +
  </div>
 +
</section>
 +
<style>
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Latest revision as of 03:54, 18 October 2018

The Collection of
SynRT
with Different Sensing Temperatures

ABSTRACT

Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'- untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.

However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their application. To address this issue, we have created a synthetic RNA-based thermosensor (SynRT) toolkit with different sensing temperatures. Additionally, we built a matched search engine for users.

The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:

SD Sequence anti-SD Sequence Ribosome

Heat-inducible RNA-based Thermosensor

RC anti-RC RNAse E

Heat-repressible RNA-based Thermosensor

Cold-inducible RNA-based Thermosensor

Stroke Version_1 RC RNAse III

Cold-repressible RNA-based Thermosensor

Learn More >

Human Practices

Several Exploration Improved

OUR TOOLKITs

Meanwhile Inspiring Us to Make

A DECADE
WORKFLOW

Hear Our Stories

Several Innovative Kits

Promoting Us to

SPREAD and DISCUSS

Synthetic Biology

Hear Our Stories

Model

Learn More >

More