Difference between revisions of "Team:Tacoma RAINmakers/Parts"

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{{Tacoma_RAINmakers}}
 
{{Tacoma_RAINmakers}}
 
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            background-image: url(https://static.igem.org/mediawiki/2018/c/c7/T--Tacoma_RAINmakers--notebookbackground.png);
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<h1>Parts</h1>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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        .container{
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
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<div class="button_link">
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
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ADD PARTS
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</a>
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<ul>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h1>Our Biobricks</h1>
 
</div>
 
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<div class="container">
  
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<h3>Parts Overview</h3>
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<br>
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    The Tacoma RAINMakers have built two biological arsenic circuits that use a combination of native E. coli and coral genes to detect arsenic ions in solution. The circuits vary in which reporter they produce, either the chromoprotein spisPink (from S. pistillata) or amilCP (from A.millepora). Each circuit contains an Arsenic regulator known as ArsR. The ArsR protein binds to a promoter with an ArsR binding site (PArsR) and inhibits the transcription of the downstream reporter gene. Only in the presence of a transcription factor, which in our case is arsenic, does the reporter become derepressed and the reporter is expressed. This circuit allows us to control chromoprotein expression using a protein sensitive to, and capable of binding with, arsenic ions.
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<br><br>
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</p>
  
  
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<h3>Submitted Parts</h3>
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<p>
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    <br>
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    </p>
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<h4>BBa_K2673000  pSB1C3/PcArsR_PArsR-spisPink</h4>
 +
    <p>
 +
 
 +
  <b>Part Type: Composite</b> <br>   
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  <b>Description: </b>
 +
    pSB1C3/PcArsR_PArsR-spisPink is an arsenic detection circuit. It is a two-part circuit made up of a series of promoter-gene sequences. The first part of the circuit, PcArsR, contains an E. coli-derived constitutive promoter (Pc), a Ribosome Binding Site, Arsenic Regulator (ArsR), and a Double Terminator. This sequence creates unregulated expression of ArsR. The second part of the circuit contains an E. coli-derived Arsenic Regulator promoter (PArsR), a Ribosome Binding Site, the spisPink chromoprotein, and a Double Terminator. The PArsR sequence has a binding site for ArsR, which blocks expression of the reporter in the absence of Arsenic ions. When Arsenic ions are present the spisPink chromoproteins are produced.
 +
        <br>
 +
  <b>Website:</b> <a href = http://parts.igem.org/Part:BBa_K2673000>http://parts.igem.org/Part:BBa_K2673000</a> <br> 
 +
      <img class= "resize" src="https://static.igem.org/mediawiki/2018/0/07/T--Tacoma_RAINmakers--SpisPinkFullCircuit.png"  alt="Spispink full circut">
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    </p>
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<h4>BBa_K2673001  pSB1C3/PcArsR_PArsR-amilCP</h4>
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    <p>
  
 +
  <b>Part Type: Composite</b> <br>   
 +
  <b>Creator: Tacoma RAINmakers</b> <br>
 +
  <b>Description: </b>
 +
    pSB1C3/PcArsR_PArsR-amilCP is an arsenic detection circuit. It is a two-part circuit that combines two series of promoter-gene sequences on one plasmid. The first part of the circuit, PcArsR, contains an E. coli-derived constitutive promoter (Pc), a Ribosome Binding Site, Arsenic Regulator (ArsR), and a Double Terminator. This sequence allows for unregulated expression of ArsR. The second part of the circuit contains an E. coli-derived Arsenic Regulator promoter (PArsR), a Ribosome Binding Site, the amilCP chromoprotein, and a Double Terminator. The PArsR sequence has a binding site for ArsR which blocks expression of the reporter in the absence of Arsenic ions. When Arsenic ions are present  amilCP chromoproteins are produced.
  
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<div class="column full_size">
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        <br>
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  <b>Website:</b> <a href = http://parts.igem.org/Part:BBa_K2673001
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>http://parts.igem.org/Part:BBa_K2673001</a> <br>       
 +
    </p>
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      <img class= "resizes" src="https://static.igem.org/mediawiki/2018/3/39/T--Tacoma_RAINmakers--AmilCPFullCircuit.png"  alt="AmilCP full circut">
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<h4>BBa_K2673002  pSB1C3/PArsR-spisPink</h4>
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    <p>
  
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<h3>What information do I need to start putting my parts on the Registry?</h3>
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  <b>Part Type: Composite</b> <br>  
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<p>The information needed to initially create a part on the Registry is:</p>
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  <b>Creator: Tacoma RAINmakers</b> <br>  
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<ul>
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  <b>Description: </b>  
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<li>Part Name</li>
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  pSB1C3/PArsR-spisPink is used to test part of the complete two-part arsenic sensing circuit. This part contains an E. coli-derived Arsenic Regulator promoter (PArsR), a Ribosome Binding Site, the spisPink chromoprotein, and a Double Terminator. The expression of the spisPink chromoprotein is regulated only if ArsR is present. In this plasmid the chromoprotein expression is unregulated.
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<li>Part type</li>
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<li>Creator</li>
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<li>Sequence</li>
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<li>Short Description (60 characters on what the DNA does)</li>
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<li>Long Description (Longer description of what the DNA does)</li>
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<li>Design considerations</li>
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</ul>
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<p>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
  
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</div>
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        <br>
 +
  <b>Website:</b> <a href =  http://parts.igem.org/Part:BBa_K2673002
 +
> http://parts.igem.org/Part:BBa_K2673002</a> <br>       
 +
    </p>
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 +
    <h4>BBa_K2673003  pSB1C3/PcArsR</h4>
 +
    <p>
  
 +
  <b>Part Type: Composite</b> <br>   
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  <b>Creator: Tacoma RAINmakers</b> <br>
 +
  <b>Description: </b>
 +
  The part contains a Ribosome Binding Site, PcArsR, and a Double Terminator. This sequence creates  unregulated expression of ArsR. There is no reporter.
 +
        <br>
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  <b>Website:</b> <a href =  http://parts.igem.org/Part:BBa_K2673003
  
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        <h4>BBa_K2673004  pSB1C3/PArsR-amilCP</h4>
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    <p>
  
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  <b>Part Type: Composite</b> <br>   
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<h3>Part Table </h3>
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  <b>Creator: Tacoma RAINmakers</b> <br>
 +
  <b>Description: </b>
 +
  pSB1C3/PArsR-amilCP is used to test part of the complete two-part arsenic circuit. This part contains an E. coli-derived ArsR promoter (PArsR), a Ribosome Binding Site, the spisPink chromoprotein, and a Double Terminator. The expression of the amilCP chromoprotein is regulated only if ArsR is present. In this plasmid the chromoprotein expression is unregulated.
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
  
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        <br>
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<groupparts>iGEM18 Tacoma_RAINmakers</groupparts>
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  <b>Website:</b> <a href =  http://parts.igem.org/Part:BBa_K2673004
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> http://parts.igem.org/Part:BBa_K2673004
  
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    </p>
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 +
        <h4>BBa_K2673005  pSB1C3/T7-amilCP
 +
</h4>
 +
    <p>
  
 +
  <b>Part Type: Composite</b> <br>   
 +
  <b>Creator: Tacoma RAINmakers</b> <br>
 +
  <b>Description: </b>
 +
To test the AmilCP promoter, we created a seperate circuit consisting of the AmilCP reporter an a high expression T7 promoter. This should produce unregulated chromoprotien, however in lab testing proved unsuccessful. We have speculated that this is due to a lack of sequence spacing around the promoter, but have yet to test this theory.
 +
 +
        <br>
 +
  <b>Website:</b> <a href =  http://parts.igem.org/Part:BBa_K2673005
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 +
 +
> http://parts.igem.org/Part:BBa_K2673005
 +
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    </p>
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<groupparts>iGEM18 Tacoma_RAINmakers</groupparts>
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Latest revision as of 18:24, 1 November 2018

Team:TacomaRAINmakers/Notebook - 2017.igem.org

Team:ECUST/Lab/Notebook

Our Biobricks

Parts Overview


The Tacoma RAINMakers have built two biological arsenic circuits that use a combination of native E. coli and coral genes to detect arsenic ions in solution. The circuits vary in which reporter they produce, either the chromoprotein spisPink (from S. pistillata) or amilCP (from A.millepora). Each circuit contains an Arsenic regulator known as ArsR. The ArsR protein binds to a promoter with an ArsR binding site (PArsR) and inhibits the transcription of the downstream reporter gene. Only in the presence of a transcription factor, which in our case is arsenic, does the reporter become derepressed and the reporter is expressed. This circuit allows us to control chromoprotein expression using a protein sensitive to, and capable of binding with, arsenic ions.

Submitted Parts


BBa_K2673000 pSB1C3/PcArsR_PArsR-spisPink

Part Type: Composite
Description: pSB1C3/PcArsR_PArsR-spisPink is an arsenic detection circuit. It is a two-part circuit made up of a series of promoter-gene sequences. The first part of the circuit, PcArsR, contains an E. coli-derived constitutive promoter (Pc), a Ribosome Binding Site, Arsenic Regulator (ArsR), and a Double Terminator. This sequence creates unregulated expression of ArsR. The second part of the circuit contains an E. coli-derived Arsenic Regulator promoter (PArsR), a Ribosome Binding Site, the spisPink chromoprotein, and a Double Terminator. The PArsR sequence has a binding site for ArsR, which blocks expression of the reporter in the absence of Arsenic ions. When Arsenic ions are present the spisPink chromoproteins are produced.
Website: http://parts.igem.org/Part:BBa_K2673000
Spispink full circut

BBa_K2673001 pSB1C3/PcArsR_PArsR-amilCP

Part Type: Composite
Creator: Tacoma RAINmakers
Description: pSB1C3/PcArsR_PArsR-amilCP is an arsenic detection circuit. It is a two-part circuit that combines two series of promoter-gene sequences on one plasmid. The first part of the circuit, PcArsR, contains an E. coli-derived constitutive promoter (Pc), a Ribosome Binding Site, Arsenic Regulator (ArsR), and a Double Terminator. This sequence allows for unregulated expression of ArsR. The second part of the circuit contains an E. coli-derived Arsenic Regulator promoter (PArsR), a Ribosome Binding Site, the amilCP chromoprotein, and a Double Terminator. The PArsR sequence has a binding site for ArsR which blocks expression of the reporter in the absence of Arsenic ions. When Arsenic ions are present amilCP chromoproteins are produced.
Website: http://parts.igem.org/Part:BBa_K2673001

AmilCP full circut

BBa_K2673002 pSB1C3/PArsR-spisPink

Part Type: Composite
Creator: Tacoma RAINmakers
Description: pSB1C3/PArsR-spisPink is used to test part of the complete two-part arsenic sensing circuit. This part contains an E. coli-derived Arsenic Regulator promoter (PArsR), a Ribosome Binding Site, the spisPink chromoprotein, and a Double Terminator. The expression of the spisPink chromoprotein is regulated only if ArsR is present. In this plasmid the chromoprotein expression is unregulated.
Website: http://parts.igem.org/Part:BBa_K2673002

BBa_K2673003 pSB1C3/PcArsR

Part Type: Composite
Creator: Tacoma RAINmakers
Description: The part contains a Ribosome Binding Site, PcArsR, and a Double Terminator. This sequence creates unregulated expression of ArsR. There is no reporter.
Website: http://parts.igem.org/Part:BBa_K2673003

BBa_K2673004 pSB1C3/PArsR-amilCP

Part Type: Composite
Creator: Tacoma RAINmakers
Description: pSB1C3/PArsR-amilCP is used to test part of the complete two-part arsenic circuit. This part contains an E. coli-derived ArsR promoter (PArsR), a Ribosome Binding Site, the spisPink chromoprotein, and a Double Terminator. The expression of the amilCP chromoprotein is regulated only if ArsR is present. In this plasmid the chromoprotein expression is unregulated.
Website: http://parts.igem.org/Part:BBa_K2673004

BBa_K2673005 pSB1C3/T7-amilCP

Part Type: Composite
Creator: Tacoma RAINmakers
Description: To test the AmilCP promoter, we created a seperate circuit consisting of the AmilCP reporter an a high expression T7 promoter. This should produce unregulated chromoprotien, however in lab testing proved unsuccessful. We have speculated that this is due to a lack of sequence spacing around the promoter, but have yet to test this theory.
Website: http://parts.igem.org/Part:BBa_K2673005



<groupparts>iGEM18 Tacoma_RAINmakers</groupparts>