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− | <source src="https://static.igem.org/mediawiki/2018/ | + | <source src="https://static.igem.org/mediawiki/2018/1/1b/T--Lambert_GA--hi.mp4" type="video/mp4"> |
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<div id="sidebar"> | <div id="sidebar"> | ||
− | <br><br><br> | + | <br><br> |
− | + | <div id="link"> | |
− | + | <b><a style="color:black; text-decoration: none; line-height:1.1;" href="#target1">RESEARCHERS</a></b> | |
− | + | </div> | |
+ | <br><br> | ||
+ | <div id="link"> | ||
+ | <b><a style="color:black; text-decoration: none; line-height:1.1;" href="#target2">PROJECT FOUNDATION</a></b> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div id="link"> | ||
+ | <b><a style="color:black; text-decoration: none; line-height:1.1;" href="#target3">SUPPORT</a></b> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div id="link"> | ||
+ | <b><a style="color:black; text-decoration: none; line-height:1.1;" href="#target4">REFERENCES</a></b> | ||
+ | </div> | ||
+ | <br><br> | ||
</div> | </div> | ||
</div> | </div> | ||
+ | |||
<div id="maincontent"> | <div id="maincontent"> | ||
− | <br><br>< | + | <br><br> |
− | + | <div id="target1"></div> | |
− | <div | + | <div id="subheading1"> |
− | < | + | <b>Researchers</b> |
− | < | + | </div> |
− | < | + | <br><br> |
+ | <div id="content2"> | ||
+ | <center> | ||
+ | <table> | ||
+ | <tr> | ||
+ | <th>Abby Bell <br> <i>Wet Lab</i></th> | ||
+ | <th>Annie Pei<br> <i>Human Practices</i></th> | ||
+ | <th>Ashtyn Cauffman <br> <i>Human Practices</i></th> | ||
+ | <th>Christina Lee<br> <i>Human Practices</i></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Courtney Taing<br> <i>Human Practices</i></th> | ||
+ | <th>Ellie Kim<br> <i>Wet Lab</i></th> | ||
+ | <th>Gaurav Byagathvalli<br> <i>ElectroPen</i></th> | ||
+ | <th>Katie Barr<br> <i>Wet Lab</i></th> | ||
+ | </tr> | ||
+ | <th>Kevin Li<br> <i>Wiki</i></th> | ||
+ | <th>Krishi Kishore<br> <i>Software</i></th> | ||
+ | <th>Megan Hong<br> <i>Wet Lab</i></th> | ||
+ | <th>Michelle Wu<br> <i>Human Practices</i></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Nithik Balachandran<br> <i>Wiki</i></th> | ||
+ | <th>Rohil Badkundri<br> <i>CALM Lead</i></th> | ||
+ | <th>Sahil Jain<br> <i>Software</i></th> | ||
+ | <th>Sai Mettupalli<br> <i>Hardware</i></th> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th>Shanthi Hegde<br> <i>Graphic Design</i></th> | ||
+ | <th>Srikusumanjali Pinnamareddy<br> <i>CALM</i></th> | ||
+ | <th>Tapasya Katta<br> <i>Human Practices</i></th> | ||
+ | <th>Victor Valbuena<br> <i>CALM</i></th> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </center> | ||
</div> | </div> | ||
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<br><br> | <br><br> | ||
− | + | <div id="target2"></div> | |
+ | <div id="subheading1"> | ||
+ | <b>Project Foundation</b> | ||
+ | </div> | ||
+ | <br><br> | ||
+ | <div id="content2"> | ||
+ | This year's project toehold switch circuit design was a result of collaborative discussions with Dr. Styczysnki, Ms. Zhang and Ms. McNerney from Georgia Institute of Technology. Lambert iGEM worked extensively with third parties for this year's project, and we would like to offer our most sincere thanks for their help. Attributions are listed below by subproject. | ||
</div> | </div> | ||
+ | <br><br> | ||
+ | <div id="target3"></div> | ||
+ | <div id="subheading1"><b>Support</b></div> | ||
+ | <br><br> | ||
+ | <div id="content2"> | ||
+ | <b style="font-size:20px;">Scientific</b> | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>Dr. Mark Styczynski from Georgia Institute of Technology</li> | ||
+ | <li>Dr. Saad Bhamla from Georgia Institute of Technology</li> | ||
+ | <li>Ms. Yan Zhang from Georgia Institute of Technology</li> | ||
+ | <li>Ms. Monica McNerney from Georgia Institute of Technology</li> | ||
+ | </ul> | ||
+ | <br> | ||
+ | Dr. Styczynski, Dr. Bhamla, Ms. Zhang and Ms. McNerney have assisted Lambert iGEM throughout their project by acting as mentors. They have helped the team troubleshoot and revise its project, constantly providing advice to improve it. In addition, they have generously provided materials that a high school team would not have access to. We would like to thank them for helping us complete our project. | ||
+ | <br><br> | ||
+ | <b style="font-size:20px;">Parts</b> | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>Sequences and initial DNA from Styczynski Lab at Georgia Tech</li> | ||
+ | <li>Snapgene- software for sequence analysis</li> | ||
+ | <li>IDT-Generous DNA synthesis</li> | ||
+ | </ul> | ||
− | < | + | <br><br> |
+ | <b style="font-size:20px;">CALM Model</b> | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>The AI Day One Challenge, coordinated by Mr. Scott Henderson through Sandbox ATL, also allowing Lambert iGEM to meet with the following:</li> | ||
+ | <li>Dr. Eric Mintz</li> | ||
+ | <li>Mr. Chris Waites</li> | ||
+ | <li>Mr. Luigi Ray-Montanez</li> | ||
+ | <li>Mr. Jackson Morgan</li> | ||
+ | <li>Mr. TJ Muehlemen</li> | ||
+ | <li>Mr. Shane Matthews</li> | ||
+ | <li>Mr. Michael Koohang</li> | ||
+ | <li>Mrs. Alexa Morse</li> | ||
+ | <li>Mr. Greg Torre</li> | ||
+ | <li>Mr. Daniel Youmans</li> | ||
+ | <li>Ms. Winne Luo</li> | ||
+ | <li>Tanishk Sinha (a student at Lambert High School) | ||
+ | </ul> | ||
+ | <br><br> | ||
+ | <b style="font-size:20px;">ElectroPen</b> | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>The ElectroPen project would not have been possible without our collaboration with the Bhamla Lab at the Georgia Institute of Technology:</li> | ||
+ | <li>Dr. Saad Bhamla</li> | ||
+ | <li>Soham Sinha</li> | ||
+ | </ul> | ||
+ | <br><br> | ||
+ | <b style="font-size:20px;">Advisor</b> | ||
+ | <br> | ||
+ | <ul> | ||
+ | <li>Mrs. Standeven</li> | ||
+ | <li>Dr. Cantrell</li> | ||
+ | </ul> | ||
− | < | + | <br><br> |
− | < | + | <b style="font-size:20px;">Outreach</b> |
+ | <br> | ||
+ | <ul> | ||
+ | <li>Dr. Rainyer- Dominican Republic</li> | ||
+ | <li>Girl Scout Troops</li> | ||
+ | <li>Riverwatch and South Forsyth Middle Schools</li> | ||
+ | <li>Atlanta Science Festival</li> | ||
+ | <li>University of Georgia (UGA) iGEM Team</li> | ||
+ | <li>Georgia State University (GSU) iGEM </li> | ||
+ | <li>Emory University iGEM </li> | ||
+ | <li>TASTaipei iGEM </li> | ||
+ | </ul> | ||
+ | <br><br> | ||
+ | <b style="font-size:20px;">Community</b> | ||
+ | <br> | ||
<ul> | <ul> | ||
− | <li> | + | <li>Open House- family members, local business owners and professionals</li> |
− | + | <li>Lambert Administrators and teachers</li> | |
− | + | <li>Dr. Christian Epe, Dr. Sammy Bell, Dr. Marcus Terneus, Dr. Dave Lowery, Dr. Tong and Boeheim Ingelheim Animal Health R&D</li> | |
− | <li> | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | <li> | + | |
</ul> | </ul> | ||
− | |||
− | < | + | <br><br> |
− | + | <b style="font-size:20px;">Fundraising and Financial Support</b> | |
+ | <br> | ||
<ul> | <ul> | ||
− | <li> | + | <li>National Science Foundation Grant #1254382</li> |
− | <li> | + | <li>Johns Creek Chipotle</li> |
− | <li> | + | <li>Teen Arnold</li> |
− | <li> | + | <li>Gibson Software</li> |
− | + | ||
− | + | ||
</ul> | </ul> | ||
− | |||
− | + | <br><br> | |
− | < | + | <b style="font-size:20px;">Presentation</b> |
− | < | + | <br> |
− | + | ||
− | + | ||
− | < | + | |
<ul> | <ul> | ||
− | <li> | + | <li>Dr. Christian Epe, Dr. Sammy Bell, Dr. Marcus Terneus, Dr. Dave Lowery, and Dr. Tong from Boeheim Ingelheim</li> |
− | <li>< | + | <li>Mike Gibson from Gibson Software</li> |
− | <li> | + | <li>Mark Styzinski, Yan Zhang, and Saad Bhamla from Georgia Tech</li> |
− | <li> | + | <li>Paul Standeven from Philips Healthcare North America</li> |
+ | <li>Dr. Dana Boyd Barr, Dr. P. Barry Ryan, Dr. Christine L Moe, and Dr. Parinya Panuwet from Emory Healthcare</li> | ||
+ | <li>Dr. Davison, Principal Lambert High School</li> | ||
</ul> | </ul> | ||
− | |||
</div> | </div> | ||
+ | <br><br> | ||
+ | <div id="target4"></div> | ||
+ | <div id="subheading2"><b>References</b></div> | ||
+ | <br><br> | ||
+ | <div id="content2"> | ||
+ | [1] Wholey, W. Y., & Jakob, U. (2012, March). Hsp33 confers bleach resistance by protecting elongation factor Tu against oxidative degradation in Vibrio cholerae. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pubmed/22296329 | ||
+ | <br> | ||
+ | [2] Cholera - Vibrio cholerae infection. (2018, July 20). Retrieved October 8, 2018, from https://www.cdc.gov/cholera/diagnosis.html | ||
+ | <br> | ||
+ | [3] Nelson, E. J., Harris, J. B., Morris, J. G., Calderwood, S. B., & Camilli, A. (2009, October). Cholera transmission: The host, pathogen and bacteriophage dynamic. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842031/ | ||
+ | <br> | ||
+ | [4] Kaye N. Ballantyne, Renato Salemi, Fabio Guarino, James R. Pearson, Dale Garlepp, Stephen Fowler & Roland A.H. van Oorschot (2015) DNA contamination minimisation – finding an effective cleaning method, Australian Journal of Forensic Sciences, 47:4, 428-439, DOI: <u>10.1080/00450618.2015.1004195</u> | ||
+ | <br> | ||
+ | [5] 10.3 - Sensitivity, Specificity, Positive Predictive Value, and Negative Predictive Value. (2018). Retrieved October 8, 2018, from https://onlinecourses.science.psu.edu/stat507/node/71/ | ||
+ | <br> | ||
+ | [6] Cholera - Vibrio cholerae infection. (2018, July 20). Retrieved from https://www.cdc.gov/cholera/diagnosis.html | ||
+ | <br> | ||
+ | [7] Nelson, E. J., Harris, J. B., Morris, J. G., Calderwood, S. B., & Camilli, A. (2009, October). Cholera transmission: The host, pathogen and bacteriophage dynamic. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842031/ | ||
+ | <br> | ||
+ | [8] Bjerketorp, J., Håkansson, S., Belkin, S., & Jansson, J. K. (2006, February). Advances in preservation methods: Keeping biosensor microorganisms alive and active. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pubmed/16368231 | ||
+ | <br> | ||
+ | [9] <i>EXAMINATION OF FOOD AND ENVIRONMENTAL SAMPLES</i> [PDF]. (n.d.). Centers for Disease Control.London School of Hygiene & Tropical Medicine. (2018, May 03). Upcoming rainy season likely to trigger renewed cholera outbreak in Yemen. Retrieved from https://www.lshtm.ac.uk/newsevents/news/2018/upcoming-rainy-season-likely-trigger-renewed-cholera-outbreak-yemen | ||
+ | <br> | ||
+ | [10] Drinking-water. (2018, February 7). Retrieved from http://www.who.int/news-room/fact- sheets/detail/drinking-water | ||
+ | (n.d.). Retrieved from http://www.who.int/water_sanitation_health/takingcharge.html | ||
+ | <br> | ||
+ | [11] Berman, J. (2009, October 29). WHO: Waterborne Disease is World's Leading Killer. Retrieved from https://www.voanews.com/a/a-13-2005-03-17-voa34-67381152/274768.html | ||
+ | <br> | ||
+ | [12] Learn How to Use the Crystal VC Dipstick Test to Detect Vibrio Cholera in Our New Video | DOVE: Stop Cholera. (n.d.). Retrieved from https://www.stopcholera.org/blog/learn-how-use-crystal-vc-dipstick-test-detect-vibrio-cholera-our-new-video | ||
+ | <br> | ||
+ | [13] Cholera - Vibrio cholerae infection. (2018, May 14). Retrieved from https://www.cdc.gov/cholera/diagnosis.html | ||
+ | <br> | ||
+ | [14]The Burden of Soil-transmitted Helminths (STH). (2011, June 06). Retrieved from https://www.cdc.gov/globalhealth/ntd/diseases/sth_burden.html | ||
+ | <br> | ||
+ | [15] Water. (2016, April 22). Retrieved from https://www.cdc.gov/parasites/water.html | ||
+ | <br> | ||
+ | [16] Collender, P. A., Kirby, A. E., Addiss, D. G., Freeman, M. C., & Remais, J. V. (2015, December). Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4679500/ | ||
+ | <br> | ||
+ | [17] Action Against Worms. (2008, February). Retrieved from http://www.who.int/neglected_diseases/preventive_chemotherapy/pctnewsletter11.pdf | ||
+ | <br> | ||
+ | [18] Pilotte, N., Papaiakovou, M., Grant, J. R., Bierwert, L. A., Llewellyn, S., McCarthy, J. S., & Williams, S. A. (n.d.). Improved PCR-Based Detection of Soil Transmitted Helminth Infections Using a Next-Generation Sequencing Approach to Assay Design. Retrieved from http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004578 | ||
+ | <br> | ||
+ | [19] <i>Detection of Cholera Toxin</i> [PDF]. (n.d.). Atlanta: Centers for Disease Control and Prevention. | ||
+ | https://www.cdc.gov/cholera/pdf/laboratory-methods-for-the-diagnosis-of-vibrio-cholerae-chapter-7.pdf | ||
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</div> | </div> | ||
+ | |||
+ | <div id="footer"> | ||
+ | <br><br> | ||
+ | <a href="https://www.facebook.com/groups/198318606966726/"><i class="fa fa-facebook" id="flogo"></i></a> | ||
+ | |||
+ | <a href="https://twitter.com/lamberths_igem?lang=en"><i class="fa fa-twitter" id="tlogo"></i></a> | ||
+ | |||
+ | <a href="https://www.instagram.com/lambertigem/"><i class="fa fa-instagram" id="ilogo"></i></a> | ||
+ | |||
</div> | </div> | ||
Latest revision as of 21:26, 2 November 2018
A T T R I B U T I O N S
Researchers
Abby Bell Wet Lab |
Annie Pei Human Practices |
Ashtyn Cauffman Human Practices |
Christina Lee Human Practices |
---|---|---|---|
Courtney Taing Human Practices |
Ellie Kim Wet Lab |
Gaurav Byagathvalli ElectroPen |
Katie Barr Wet Lab |
Kevin Li Wiki |
Krishi Kishore Software |
Megan Hong Wet Lab |
Michelle Wu Human Practices |
Nithik Balachandran Wiki |
Rohil Badkundri CALM Lead |
Sahil Jain Software |
Sai Mettupalli Hardware |
Shanthi Hegde Graphic Design |
Srikusumanjali Pinnamareddy CALM |
Tapasya Katta Human Practices |
Victor Valbuena CALM |
Project Foundation
This year's project toehold switch circuit design was a result of collaborative discussions with Dr. Styczysnki, Ms. Zhang and Ms. McNerney from Georgia Institute of Technology. Lambert iGEM worked extensively with third parties for this year's project, and we would like to offer our most sincere thanks for their help. Attributions are listed below by subproject.
Support
Scientific
Dr. Styczynski, Dr. Bhamla, Ms. Zhang and Ms. McNerney have assisted Lambert iGEM throughout their project by acting as mentors. They have helped the team troubleshoot and revise its project, constantly providing advice to improve it. In addition, they have generously provided materials that a high school team would not have access to. We would like to thank them for helping us complete our project.
Parts
CALM Model
ElectroPen
Advisor
Outreach
Community
Fundraising and Financial Support
Presentation
- Dr. Mark Styczynski from Georgia Institute of Technology
- Dr. Saad Bhamla from Georgia Institute of Technology
- Ms. Yan Zhang from Georgia Institute of Technology
- Ms. Monica McNerney from Georgia Institute of Technology
Dr. Styczynski, Dr. Bhamla, Ms. Zhang and Ms. McNerney have assisted Lambert iGEM throughout their project by acting as mentors. They have helped the team troubleshoot and revise its project, constantly providing advice to improve it. In addition, they have generously provided materials that a high school team would not have access to. We would like to thank them for helping us complete our project.
Parts
- Sequences and initial DNA from Styczynski Lab at Georgia Tech
- Snapgene- software for sequence analysis
- IDT-Generous DNA synthesis
CALM Model
- The AI Day One Challenge, coordinated by Mr. Scott Henderson through Sandbox ATL, also allowing Lambert iGEM to meet with the following:
- Dr. Eric Mintz
- Mr. Chris Waites
- Mr. Luigi Ray-Montanez
- Mr. Jackson Morgan
- Mr. TJ Muehlemen
- Mr. Shane Matthews
- Mr. Michael Koohang
- Mrs. Alexa Morse
- Mr. Greg Torre
- Mr. Daniel Youmans
- Ms. Winne Luo
- Tanishk Sinha (a student at Lambert High School)
ElectroPen
- The ElectroPen project would not have been possible without our collaboration with the Bhamla Lab at the Georgia Institute of Technology:
- Dr. Saad Bhamla
- Soham Sinha
Advisor
- Mrs. Standeven
- Dr. Cantrell
Outreach
- Dr. Rainyer- Dominican Republic
- Girl Scout Troops
- Riverwatch and South Forsyth Middle Schools
- Atlanta Science Festival
- University of Georgia (UGA) iGEM Team
- Georgia State University (GSU) iGEM
- Emory University iGEM
- TASTaipei iGEM
Community
- Open House- family members, local business owners and professionals
- Lambert Administrators and teachers
- Dr. Christian Epe, Dr. Sammy Bell, Dr. Marcus Terneus, Dr. Dave Lowery, Dr. Tong and Boeheim Ingelheim Animal Health R&D
Fundraising and Financial Support
- National Science Foundation Grant #1254382
- Johns Creek Chipotle
- Teen Arnold
- Gibson Software
Presentation
- Dr. Christian Epe, Dr. Sammy Bell, Dr. Marcus Terneus, Dr. Dave Lowery, and Dr. Tong from Boeheim Ingelheim
- Mike Gibson from Gibson Software
- Mark Styzinski, Yan Zhang, and Saad Bhamla from Georgia Tech
- Paul Standeven from Philips Healthcare North America
- Dr. Dana Boyd Barr, Dr. P. Barry Ryan, Dr. Christine L Moe, and Dr. Parinya Panuwet from Emory Healthcare
- Dr. Davison, Principal Lambert High School
References
[1] Wholey, W. Y., & Jakob, U. (2012, March). Hsp33 confers bleach resistance by protecting elongation factor Tu against oxidative degradation in Vibrio cholerae. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pubmed/22296329
[2] Cholera - Vibrio cholerae infection. (2018, July 20). Retrieved October 8, 2018, from https://www.cdc.gov/cholera/diagnosis.html
[3] Nelson, E. J., Harris, J. B., Morris, J. G., Calderwood, S. B., & Camilli, A. (2009, October). Cholera transmission: The host, pathogen and bacteriophage dynamic. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842031/
[4] Kaye N. Ballantyne, Renato Salemi, Fabio Guarino, James R. Pearson, Dale Garlepp, Stephen Fowler & Roland A.H. van Oorschot (2015) DNA contamination minimisation – finding an effective cleaning method, Australian Journal of Forensic Sciences, 47:4, 428-439, DOI: 10.1080/00450618.2015.1004195
[5] 10.3 - Sensitivity, Specificity, Positive Predictive Value, and Negative Predictive Value. (2018). Retrieved October 8, 2018, from https://onlinecourses.science.psu.edu/stat507/node/71/
[6] Cholera - Vibrio cholerae infection. (2018, July 20). Retrieved from https://www.cdc.gov/cholera/diagnosis.html
[7] Nelson, E. J., Harris, J. B., Morris, J. G., Calderwood, S. B., & Camilli, A. (2009, October). Cholera transmission: The host, pathogen and bacteriophage dynamic. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3842031/
[8] Bjerketorp, J., Håkansson, S., Belkin, S., & Jansson, J. K. (2006, February). Advances in preservation methods: Keeping biosensor microorganisms alive and active. Retrieved October 8, 2018, from https://www.ncbi.nlm.nih.gov/pubmed/16368231
[9] EXAMINATION OF FOOD AND ENVIRONMENTAL SAMPLES [PDF]. (n.d.). Centers for Disease Control.London School of Hygiene & Tropical Medicine. (2018, May 03). Upcoming rainy season likely to trigger renewed cholera outbreak in Yemen. Retrieved from https://www.lshtm.ac.uk/newsevents/news/2018/upcoming-rainy-season-likely-trigger-renewed-cholera-outbreak-yemen
[10] Drinking-water. (2018, February 7). Retrieved from http://www.who.int/news-room/fact- sheets/detail/drinking-water (n.d.). Retrieved from http://www.who.int/water_sanitation_health/takingcharge.html
[11] Berman, J. (2009, October 29). WHO: Waterborne Disease is World's Leading Killer. Retrieved from https://www.voanews.com/a/a-13-2005-03-17-voa34-67381152/274768.html
[12] Learn How to Use the Crystal VC Dipstick Test to Detect Vibrio Cholera in Our New Video | DOVE: Stop Cholera. (n.d.). Retrieved from https://www.stopcholera.org/blog/learn-how-use-crystal-vc-dipstick-test-detect-vibrio-cholera-our-new-video
[13] Cholera - Vibrio cholerae infection. (2018, May 14). Retrieved from https://www.cdc.gov/cholera/diagnosis.html
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