Difference between revisions of "Team:Vilnius-Lithuania/Composite"

 
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<h1 class="text-wall-heading">Composite Parts</h1>
 
<h1 class="text-wall-heading">Composite Parts</h1>
 
<div class="text-wall-area-box">
 
<div class="text-wall-area-box">
 
     <h2 class="text-wall-area-box-heading">Making Them Stronger</h2>
 
     <h2 class="text-wall-area-box-heading">Making Them Stronger</h2>
 
     <div class="scroll-area">
 
     <div class="scroll-area">
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         <p class="text-content">Cell-free systems are becoming an increasingly popular in vitro tool to study biological processes as it is accompanied by less intrinsic and extrinsic noise. Relying on fundamental concepts of synthetic biology, we apply a bottom-up forward engineering approach to create a novel cell-free system for unorthodox protein-evolution. The core of this system is cell-sized liposomes that serve as excellent artificial membrane models. By encapsulating genetic material and full in vitro protein transcription and translation systems within the liposomes, we create reliable and incredibly efficient nanofactories for the production of target proteins. Even though there are many alternative proteins that can be synthesized, our main focus is directed towards membrane proteins, which occupy approximately one third of living-cells’ genomes. Considering their significance, membrane proteins are spectacularly understudied since synthesis and thus characterization of them remain prevailing obstacles to this day. We aim to utilize liposomes as nanofactories for directed evolution of membrane proteins. Furthermore, by means of directed membrane protein-evolution, a universal exposition system will be designed in order to display any protein of interest on the surface of the liposome. This way, a system is built where a phenotype of a particular protein is expressed on the outside while containing its genotype within the liposome. To prove the concept, small antibody fragments will be displayed to create a single-chain variable fragment (scFv) library for rapid screening of any designated target.</p>
+
         <p class="text-content">Composite pats that we created are used to build beta-barrel bearing protein synthesis and membrane insertion system. These parts encode recombinant proteins, that are are targeted to lipid bilayers to display different classes of antibodies.</p>
  
 
         </p>
 
         </p>
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     <div class="modal-close"></div>
 
     <div class="modal-close"></div>
 
     <div class="modal-content">
 
     <div class="modal-content">
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        <h1>Description</h1>
+
    <h1>Composite Parts</h1>
−
         <p></p>
+
    <table class="c39">
−
        <p></p>
+
         <tbody>
−
        <h2>What is SynORI?</h2>
+
          <tr class="c9">
−
        <p>SynORI stands for synthetic origin of replication. It is a framework designed to make working with single
+
              <td class="c27" colspan="1" rowspan="1">
−
            and multi-plasmid systems precise, easy and on top of that - more functional.</p>
+
                <p class="c1"><span class="c12 c29">Part Number</span></p>
−
        <p>The SynORI framework enables scientists to build a multi-plasmid system in a standardized manner by:</p>
+
              </td>
−
        <ol>
+
              <td class="c10" colspan="1" rowspan="1">
−
            <li>Selecting the number of plasmid groups</li>
+
                <p class="c1"><span class="c12 c29">Type</span></p>
−
            <li>Choosing the copy number of each group</li>
+
              </td>
−
            <li>Picking the type of copy number control (specific to one group or regulating all of them at once).</li>
+
              <td class="c17" colspan="1" rowspan="1">
−
 
+
                <p class="c1"><span class="c12 c29">Name</span></p>
−
        </ol>
+
              </td>
−
        </p>
+
              <td class="c30" colspan="1" rowspan="1">
−
 
+
                <p class="c1"><span class="c12 c29">Brief description</span></p>
−
        <p></p>
+
              </td>
−
        <p>The framework also includes a possibility of adding a selection system that reduces the usage of antibiotics
+
              <td class="c41" colspan="1" rowspan="1">
−
            (only 1 antibiotic for up to 5 different plasmids!) and an active partitioning system to make sure that low
+
                <p class="c1"><span class="c12 c29">Length, nt</span></p>
−
            copy number plasmid groups are not lost during the division.
+
              </td>
−
        </p>
+
          </tr>
−
        <p></p>
+
          <tr class="c9">
−
        <div class="img-cont">
+
              <td class="c27" colspan="1" rowspan="1">
−
            <img src="https://static.igem.org/mediawiki/parts/8/84/Collect.png" alt="img">
+
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622025"> BBa_2622025</a></span></p>
−
            <div class="img-label">
+
              </td>
−
            </div>
+
              <td class="c10" colspan="1" rowspan="1">
−
        </div>
+
                <p class="c1"><span class="c0">Composite</span></p>
−
        <h2>Applications</h2>
+
              </td>
−
        <p>
+
              <td class="c17" colspan="1" rowspan="1">
−
            <h5>Everyday lab work</h5>
+
                <p class="c1"><span class="c0">GJ6-His-GFP</span></p>
−
            <p>
+
              </td>
−
                A multi-plasmid system that is easy to assemble and control. With our framework the need to limit your
+
              <td class="c30" colspan="1" rowspan="1">
−
                research to a particular plasmid copy number just because there are not enough right replicons to
+
                <p class="c1"><span class="c0">Recombinant N-terminus histidine tagged GFP with temperature-sensitive non-coding RNA 3</span></p>
−
                choose from, is eliminated. With SynORI you can easily create a vector with a desired copy number that
+
              </td>
−
                suits your needs.</li>
+
              <td class="c31" colspan="1" rowspan="1">
−
            </p>
+
                <p class="c4"><span class="c0">809</span></p>
−
            <h5>Biological computing</h5>
+
              </td>
−
            <p>
+
          </tr>
−
                The ability to choose a wide range of copy number options and their control types will make the
+
          <tr class="c20">
−
                synthetic biology engineering much more flexible and predictable. Introduction of plasmid copy number
+
              <td class="c27" colspan="1" rowspan="1">
−
                regulation is equivalent to adding a global parameter to a computer system. It enables the coordination
+
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K26220326"> BBa_2622026</a></span></p>
−
                of multiple gene group expression.
+
              </td>
−
            </p>
+
              <td class="c10" colspan="1" rowspan="1">
−
            <h5>Smart assembly of large protein complexes</h5>
+
                <p class="c1"><span class="c0">Composite</span></p>
−
            <p>
+
              </td>
−
                The co-expression of multi-subunit complexes using different replicons brings incoherency to an already
+
              <td class="c17" colspan="1" rowspan="1">
−
                chaotic cell system. This can be avoided by using SynORI, as in this framework every plasmid group uses
+
                <p class="c1"><span class="c0">Lpp-OmpA-His</span></p>
−
                the same type of control, and in addition can act in a group-specific manner.</p>
+
              </td>
−
 
+
              <td class="c30" colspan="1" rowspan="1">
−
            <h5>Metabolic engineering</h5>
+
                <p class="c1"><span class="c0">Recombinant C-terminus histidine tagged OmpA fused with Lpp </span></p>
−
            <p>
+
              </td>
−
                A big challenge for heterologous expression of multiple gene pathways is to accurately adjust the
+
              <td class="c31" colspan="1" rowspan="1">
−
                levels of each enzyme to achieve optimal production efficiency. Precise promoter tuning in
+
                <p class="c4"><span>597</span></p>
−
                transcriptional control and synthetic ribosome binding sites in translational control are already
+
              </td>
−
                widely used to maintain expression levels. In addition to current approaches, our framework allows a
+
          </tr>
−
                simultaneous multiple gene control. Furthermore, an inducible regulation that we offer, can make the
+
          <tr class="c46">
−
                search for perfect conditions a lot easier.
+
              <td class="c27" colspan="1" rowspan="1">
−
 
+
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622027"> BBa_2622027</a></span></p>
−
 
+
              </td>
−
 
+
              <td class="c10" colspan="1" rowspan="1">
−
            </p>
+
                <p class="c1"><span class="c0">Composite</span></p>
−
 
+
              </td>
−
 
+
              <td class="c17" colspan="1" rowspan="1">
−
        </p>
+
                <p class="c1"><span class="c0">Mstx-Lpp-OmpA-His</span></p>
−
        <p>
+
              </td>
−
        </p>
+
              <td class="c30" colspan="1" rowspan="1">
−
        <table style="width:100%">
+
                <p class="c1"><span class="c0">Recombinant C-terminus histidine tagged OmpA fused with Mistic and Lpp </span></p>
−
<thead>
+
              </td>
−
<td align='center'>Species sign in ODE system</td>
+
              <td class="c31" colspan="1" rowspan="1">
−
<td align='center'>Species</td>
+
                <p class="c4"><span>933</span></p>
−
<td align='center'>Initial concentration (M)</td>
+
              </td>
−
</thead>
+
          </tr>
−
<tbody>
+
          <tr class="c35">
−
<tr>
+
              <td class="c27" colspan="1" rowspan="1">
−
<td align='center'>A</td>
+
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622028"> BBa_2622028</a></span></p>
−
<td align='center'>pDNA+RNA I+RNAII early</td>
+
              </td>
−
<td align='center'>0</td>
+
              <td class="c10" colspan="1" rowspan="1">
−
</tr>
+
                <p class="c1"><span class="c0">Composite</span></p>
−
<tr>
+
              </td>
−
<td align='center'>B</td>
+
              <td class="c17" colspan="1" rowspan="1">
−
<td align='center'>pDNA+RNA II short</td>
+
                <p class="c1"><span class="c0">Mstx-Lpp-OmpA-GFPN</span></p>
−
<td align='center'>0</td>
+
              </td>
−
</tr>
+
              <td class="c30" colspan="1" rowspan="1">
−
<tr>
+
                <p class="c1"><span class="c0">Recombinant protein OmpA fused with Mistic, Lpp and GFP Nanobody</span></p>
−
<td align='center'>RNAI</td>
+
              </td>
−
<td align='center'>RNA I</td>
+
              <td class="c31" colspan="1" rowspan="1">
−
<td align='center'>1E-6</td>
+
                <p class="c4"><span class="c0">1272</span></p>
−
</tr>
+
              </td>
−
<tr>
+
          </tr>
−
<td align='center'>D</td>
+
          <tr class="c45">
−
<td align='center'>pDNA+RNA II long</td>
+
              <td class="c27" colspan="1" rowspan="1">
−
<td align='center'>0</td>
+
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622029"> BBa_2622029</a></span></p>
−
</tr>
+
              </td>
−
<tr>
+
              <td class="c10" colspan="1" rowspan="1">
−
<td align='center'>E</td>
+
                <p class="c1"><span class="c0">Composite</span></p>
−
<td align='center'>pDNA+RNAII primer</td>
+
              </td>
−
<td align='center'>0</td>
+
              <td class="c17" colspan="1" rowspan="1">
−
</tr>
+
                <p class="c1"><span class="c0">Lpp-OmpA-GFPN</span></p>
−
<tr>
+
              </td>
−
<td align='center'>F</td>
+
              <td class="c30" colspan="1" rowspan="1">
−
<td align='center'>RNA II long</td>
+
                <p class="c1"><span class="c0">Recombinant protein OmpA fused with Lpp and GFP Nanobody</span></p>
−
<td align='center'>0</td>
+
              </td>
−
</tr>
+
              <td class="c31" colspan="1" rowspan="1">
−
<tr>
+
                <p class="c4"><span class="c0">936</span></p>
−
<td align='center'>G</td>
+
              </td>
−
<td align='center'>pDNA</td>
+
          </tr>
−
<td align='center'>4E-8*</td>
+
          <tr class="c28">
−
</tr>
+
              <td class="c27" colspan="1" rowspan="1">
−
<tr>
+
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622030"> BBa_2622030</a></span></p>
−
<td align='center'>H</td>
+
              </td>
−
<td align='center'>pDNA+RNA II+RNA I late</td>
+
              <td class="c10" colspan="1" rowspan="1">
−
<td align='center'>0</td>
+
                <p class="c1"><span class="c0">Composite</span></p>
−
</tr>
+
              </td>
−
<tr>
+
              <td class="c17" colspan="1" rowspan="1">
−
<td align='center'>RNA II</td>
+
                <p class="c1"><span class="c0">Lpp-OmpA-scFv_antiVLY</span></p>
−
<td align='center'>RNA II</td>
+
              </td>
−
<td align='center'>0</td>
+
              <td class="c30" colspan="1" rowspan="1">
−
</tr>
+
                <p class="c1"><span class="c0">Recombinant protein OmpA fused with Lpp and scFv_antiVLY</span></p>
−
<tr>
+
              </td>
−
<td align='center'>J</td>
+
              <td class="c31" colspan="1" rowspan="1">
−
<td align='center'>RNAI+RNAII</td>
+
                <p class="c4"><span class="c0">1317</span></p>
−
<td align='center'>0</td>
+
              </td>
−
</tr>
+
          </tr>
−
</tbody>
+
          <tr class="c28">
−
</table>
+
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622031"> BBa_2622031</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Mstx-Lpp-OmpA-scFv_antiVLY</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Recombinant protein OmpA fused with Mistic, Lpp and scFv_antiVLY</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1653</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622032"> BBa_2622032</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">His-IgA-Mstx</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Recombinant N-terminus histidine tagged IgA protein fused with Mistic</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">2001</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622033"> BBa_2622033</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">His-scFv_antiVLY</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">N-terminus histidine tagged scFv_antiVLY</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">927</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c28">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622034"> BBa_2622034</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">GFPN-IgA-Mstx</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Recombinant protein IgA fused with GFP Nanobody and Mistic</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">2286</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c28">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622035"> BBa_2622035</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">scFv_antiVGY-IgA-Mstx</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Recombinant protein IgA fused with scFv_antiVLY and Mistic</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">2667</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c28">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622036"> BBa_2622036</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Sw11-OmpA-His</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">C-terminus histidine tagged OmpA with temperature-sensitive non-coding RNA 11</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">598</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c28">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622037"> BBa_2622037</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Sw11-Mstx-OmpA-His</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Recombinant C-terminus histidine tagged OmpA fused with temperature-sensitive non-coding RNA 11 and Mistic </span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1082</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c28">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622038"> BBa_2622038</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Mstx-scFv_antiVLY</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Recombinant protein scFv_antiVLY fused with Mistic</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1266</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622039"> BBa_2622039</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamA-His composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">C-terminus histidine tagged BamA</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">2569</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622040"> BBa_2622040</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamA composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">C-terminus histidine tagged BamA controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">2580</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622041"> BBa_2622041</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamA composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamA controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">2628</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622042"> BBa_2622042</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamB composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamB controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1369</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622043"> BBa_2622043</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamC composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamC controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1165</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622044"> BBa_2622044</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamD composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamD controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">865</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622045"> BBa_2622045</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamE composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">BamE controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">556</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622046"> BBa_2622046</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">SurA composite</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">SurA controlled by T7 promoter and terminator</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1482</span></p>
 +
              </td>
 +
          </tr>
 +
          <tr class="c9">
 +
              <td class="c27" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0"><a href="http://parts.igem.org/Part:BBa_K2622047"> BBa_2622047</a></span></p>
 +
              </td>
 +
              <td class="c10" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Composite</span></p>
 +
              </td>
 +
              <td class="c17" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Mstx-OmpA_full-His</span></p>
 +
              </td>
 +
              <td class="c30" colspan="1" rowspan="1">
 +
                <p class="c1"><span class="c0">Full length recombinant C-terminus histidine tagged protein OmpA fused with Mistic</span></p>
 +
              </td>
 +
              <td class="c31" colspan="1" rowspan="1">
 +
                <p class="c4"><span class="c0">1340</span></p>
 +
              </td>
 +
          </tr>
 +
        </tbody>
 +
    </table>
 
     </div>
 
     </div>
 
</div>
 
</div>

Latest revision as of 20:35, 4 November 2018

Composite Parts

Making Them Stronger

Composite pats that we created are used to build beta-barrel bearing protein synthesis and membrane insertion system. These parts encode recombinant proteins, that are are targeted to lipid bilayers to display different classes of antibodies.

Composite Parts

Part Number

Type

Name

Brief description

Length, nt

BBa_2622025

Composite

GJ6-His-GFP

Recombinant N-terminus histidine tagged GFP with temperature-sensitive non-coding RNA 3

809

BBa_2622026

Composite

Lpp-OmpA-His

Recombinant C-terminus histidine tagged OmpA fused with Lpp

597

BBa_2622027

Composite

Mstx-Lpp-OmpA-His

Recombinant C-terminus histidine tagged OmpA fused with Mistic and Lpp

933

BBa_2622028

Composite

Mstx-Lpp-OmpA-GFPN

Recombinant protein OmpA fused with Mistic, Lpp and GFP Nanobody

1272

BBa_2622029

Composite

Lpp-OmpA-GFPN

Recombinant protein OmpA fused with Lpp and GFP Nanobody

936

BBa_2622030

Composite

Lpp-OmpA-scFv_antiVLY

Recombinant protein OmpA fused with Lpp and scFv_antiVLY

1317

BBa_2622031

Composite

Mstx-Lpp-OmpA-scFv_antiVLY

Recombinant protein OmpA fused with Mistic, Lpp and scFv_antiVLY

1653

BBa_2622032

Composite

His-IgA-Mstx

Recombinant N-terminus histidine tagged IgA protein fused with Mistic

2001

BBa_2622033

Composite

His-scFv_antiVLY

N-terminus histidine tagged scFv_antiVLY

927

BBa_2622034

Composite

GFPN-IgA-Mstx

Recombinant protein IgA fused with GFP Nanobody and Mistic

2286

BBa_2622035

Composite

scFv_antiVGY-IgA-Mstx

Recombinant protein IgA fused with scFv_antiVLY and Mistic

2667

BBa_2622036

Composite

Sw11-OmpA-His

C-terminus histidine tagged OmpA with temperature-sensitive non-coding RNA 11

598

BBa_2622037

Composite

Sw11-Mstx-OmpA-His

Recombinant C-terminus histidine tagged OmpA fused with temperature-sensitive non-coding RNA 11 and Mistic

1082

BBa_2622038

Composite

Mstx-scFv_antiVLY

Recombinant protein scFv_antiVLY fused with Mistic

1266

BBa_2622039

Composite

BamA-His composite

C-terminus histidine tagged BamA

2569

BBa_2622040

Composite

BamA composite

C-terminus histidine tagged BamA controlled by T7 promoter and terminator

2580

BBa_2622041

Composite

BamA composite

BamA controlled by T7 promoter and terminator

2628

BBa_2622042

Composite

BamB composite

BamB controlled by T7 promoter and terminator

1369

BBa_2622043

Composite

BamC composite

BamC controlled by T7 promoter and terminator

1165

BBa_2622044

Composite

BamD composite

BamD controlled by T7 promoter and terminator

865

BBa_2622045

Composite

BamE composite

BamE controlled by T7 promoter and terminator

556

BBa_2622046

Composite

SurA composite

SurA controlled by T7 promoter and terminator

1482

BBa_2622047

Composite

Mstx-OmpA_full-His

Full length recombinant C-terminus histidine tagged protein OmpA fused with Mistic

1340
invert