|
|
| (4 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| − | <html>
| + | {{Washington/Base}} |
| − | | + | <html></html> |
| − | <!-- Set up mobile viewport and meta tag -->
| + | |
| − | <meta charset="utf-8">
| + | |
| − | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1.0, user-scalable=no, shrink-to-fit=no">
| + | |
| − | | + | |
| − | <!-- Import CSS -->
| + | |
| − | <link href="https://2018.igem.org/Team:Washington/CSS?action=raw&ctype=text/css" type="text/css" rel="stylesheet" />
| + | |
| − | | + | |
| − | <!-- Create root div for React to inject information into -->
| + | |
| − | <div id="root">Please enable JavaScript to view this page.</div>
| + | |
| − | | + | |
| − | <!-- Add on-page javascript for destroying default iGEM CSS and adding a loading animation -->
| + | |
| − | <!-- I do it twice to run once so the page loading animation gets correct styling the first run -->
| + | |
| − | <!-- Second run is just to make sure everything gets ran -->
| + | |
| − | <script type="text/javascript">
| + | |
| − | $('div#content').removeAttr('id').attr('id', 'contentBox');
| + | |
| − | $('a#top').remove();
| + | |
| − | $('div#top_title').remove();
| + | |
| − | $('div#HQ_page').removeAttr('id');
| + | |
| − | $('div.mw-body').removeAttr('class');
| + | |
| − | $('div#mw-content-text').removeAttr('id');
| + | |
| − | $('div#bodyContent').removeAttr('id');
| + | |
| − | $('div.mw-content-ltr>p').addClass('meta');
| + | |
| − | $('div.mw-content-ltr').removeAttr('class');
| + | |
| − | document.getElementById("root").innerText = "Page loading...";
| + | |
| − | $(document).ready(function () {
| + | |
| − | $('div#content').removeAttr('id').attr('id', 'contentBox');
| + | |
| − | $('a#top').remove();
| + | |
| − | $('div#top_title').remove();
| + | |
| − | $('div#HQ_page').removeAttr('id');
| + | |
| − | $('div.mw-body').removeAttr('class');
| + | |
| − | $('div#mw-content-text').removeAttr('id');
| + | |
| − | $('div#bodyContent').removeAttr('id');
| + | |
| − | $('div.mw-content-ltr>p').addClass('meta');
| + | |
| − | $('div.mw-content-ltr').removeAttr('class');
| + | |
| − | })
| + | |
| − | </script> | + | |
| − | | + | |
| − | <!-- Import React Javascript -->
| + | |
| − | <script src="https://2018.igem.org/Team:Washington/Javascript?action=raw&ctype=text/javascript" type="text/javascript"></script>
| + | |
| − | | + | |
| − | </html> | + | |
Please enable JavaScript to view this page. If you are viewing this page with javascript enabled, please contact wkwok16@uw.edu and send a screenshot of the developer console. Here is our project abstract:
Chemically Induced Dimerization of Nanobodies for the Development of Versatile Biosensors
Chemically induced dimerization (CID), in which two proteins dimerize only in the presence of a small molecule, has been widely used to control cell signaling, regulatory, and metabolic pathways, and used as logic gates for biological computation in living mammalian cells. However, few naturally occuring CID systems and their derivatives are currently available. Creating a CID system with desired affinity and specificity for any given small molecule remains an unsolved problem for computational design and other protein engineering approaches. To address this challenge, we have used a novel strategy to select CID binders from a vastly diverse combinatorial nanobody library. We have created new CID systems that can sense cholecalciferol and artemisinin. We are validating CID biosensors by a yeast three-hybrid system and built structural models to understand the small molecule-induced dimerization. Our work is a proof-of-concept that can be generalized to create CID systems for many applications.
