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| − | <div class="column full_size" >
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| − | <h1> Welcome to iGEM 2018! </h1>
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| − | <p>Your team has been approved and you are ready to start the iGEM season! </p>
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| − | <img src="http://placehold.it/1080x320/c4baba/e4dede">
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| − | <h3>Before you start</h3>
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| − | <p> Please read the following pages:</p>
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| − | <li> <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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| − | <li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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| − | </ul>
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| − | <h3> Styling your wiki </h3>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <h3> Uploading pictures and files </h3>
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| − | <p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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| − | <p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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| − | <div class="button_link">
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| − | <a href="https://2018.igem.org/Special:Upload">
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| − | UPLOAD FILES
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| − | <h3> Wiki template information </h3>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <h3> Editing your wiki </h3>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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| − | <div class="button_link">
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| − | <a href="https://2018.igem.org/wiki/index.php?title=Team:Washington&action=edit">
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| − | EDIT PAGE
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| − | </a>
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| − | <h3>Tips</h3>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | <h3>Inspiration</h3>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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Please enable JavaScript to view this page. If you are viewing this page with javascript enabled, please contact wkwok16@uw.edu and send a screenshot of the developer console. Here is our project abstract:
Chemically Induced Dimerization of Nanobodies for the Development of Versatile Biosensors
Chemically induced dimerization (CID), in which two proteins dimerize only in the presence of a small molecule, has been widely used to control cell signaling, regulatory, and metabolic pathways, and used as logic gates for biological computation in living mammalian cells. However, few naturally occuring CID systems and their derivatives are currently available. Creating a CID system with desired affinity and specificity for any given small molecule remains an unsolved problem for computational design and other protein engineering approaches. To address this challenge, we have used a novel strategy to select CID binders from a vastly diverse combinatorial nanobody library. We have created new CID systems that can sense cholecalciferol and artemisinin. We are validating CID biosensors by a yeast three-hybrid system and built structural models to understand the small molecule-induced dimerization. Our work is a proof-of-concept that can be generalized to create CID systems for many applications.
