Difference between revisions of "Team:SSTi-SZGD/Improve"

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This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
 
This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
 
<!--{cn}今年，我们共提交了7个新的部分，包括4个基本部分和3个综合部分。我们利用BGI中国公司今年8月推出的特殊基因合成产品来合成我们的基础部件，分别合成了编码序列、RBS、启动子和信号肽等基本部分。采用聚合酶链反应(PCR)、限制性内切酶连接和3a装配相结合的方法制备复合构件。所有提交的部件都与RFC 10兼容。-->
 
<!--{cn}今年，我们共提交了7个新的部分，包括4个基本部分和3个综合部分。我们利用BGI中国公司今年8月推出的特殊基因合成产品来合成我们的基础部件，分别合成了编码序列、RBS、启动子和信号肽等基本部分。采用聚合酶链反应(PCR)、限制性内切酶连接和3a装配相结合的方法制备复合构件。所有提交的部件都与RFC 10兼容。-->
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<!--Improved part-->
 
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<section id="Improved_part" class="Improved_part col-xs-12 col-md-10">
 
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<div class="subtitle">
 
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<span>Improved parts<!--{cn}改进部分--></span>
 
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</div>
 
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<div class="content abstract">
 
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In our project, we added to the characterization of 
 
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<!--{cn}在我们的项目中，我们添加了现有的-->
 
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<a href="http://parts.igem.org/Part:BBa_K1469009">an existing Biobrick<!--{cn}BioBrick的特性--></a>
 
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, design by Philip Hartz from iGEM Saarland 2014 . Our P43-tuaD-gtaB-T1 composite part was designed based on the 
 
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<!--{cn}，由2014年iGEM Saarland的Philip Hartz设计。我们的p43-tuad-gtab-t1复合部件是在Saarland的-->
 
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<a href="http://parts.igem.org/Part:BBa_K2593008">BioBrick created by<!--{cn}BioBrick基础上设计的--></a>
 
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Saarland with some alterations . For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant
 
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<!--{cn}，并作了一些改动。例如，将tuad-gtab作为操纵子的共同表达作为控制下的一个本构启动子以至于重组-->
 
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<i>B. Subtilis 168<!--{cn}枯草芽孢杆菌168--></i>
 
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produces elevated level of HA.
 
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<!--{cn}产生较高水平的透明质酸。-->
 
 
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</section>
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<!--Improved part-->
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<section id="Improved_part" class="Improved_part col-xs-12 col-md-10">
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<div class="subtitle">
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<span>Improved parts<!--{cn}改进部分--></span>
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</div>
 +
 +
<div class="content abstract">
 +
In our project, we added to the characterization of 
 +
<!--{cn}在我们的项目中，我们添加了现有的-->
 +
<a href="http://parts.igem.org/Part:BBa_K1469009">an existing Biobrick<!--{cn}生物砖块--></a>
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, design by Philip Hartz from iGEM Saarland 2014. Our P43-tuaD-gtaB-T1 composite part was designed based on two BioBrick parts (
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<!--{cn}，设计由菲利普·哈茨从萨尔iGEM大赛2014年的表征。我们的P43-tuaD-gtaB-T1复合部件是基于两个BioBrick组件设计（-->
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<a href="http://parts.igem.org/Part:BBa_K1469002">BBa_K1469002</a>
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and<!--{cn}和-->
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<a href="http://parts.igem.org/Part:BBa_K1469005">BBa_K1469005</a>
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) created by Saarland. For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant <!--{cn}）例如，将tuad-gtab作为操纵子的共同表达作为控制下的一个本构启动子以至于重组。例如，将tuad-gtab作为操纵子的共同表达作为控制下的一个本构启动子以至于重组-->
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<i>B. Subtilis 168<!--{cn}枯草芽孢杆菌168--></i>
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produces elevated level of HA.<!--{cn}产生较高水平的透明质酸。-->
 
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Latest revision as of 14:54, 6 November 2018

Overview
This year, we submitted seven new parts in total, including four basic parts and three composite parts. We took advantage on the BGI China special gene synthesis promotion offer in August this year to synthesize our basic parts, such as coding sequence, RBS, promoter and signal peptide separately. The composite parts were generated using a combination of PCR, restriction ligation and 3A assembly methods. All submitted parts are RFC10 compatible.
All parts
Name Type Description Designer Length
BBa_K2593001 coding Hyaluronic acid synthase protein Hong Chen 1254
BBa_K2593003 coding Signal peptide Hong Chen 99
BBa_K2593004 coding Leech Hyaluronic acid hydrolase protein (58KDa) Huimin Li 1470
BBa_K2593009 coding Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus Huimin Li 1686
BBa_K2593006 composite Leech Hyaluronic acid hydrolase with inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator Yongtao Lin 1962
BBa_K2593007 composite Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. Yongtao Lin 2672
BBa_K2593008 composite UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. Hong Chen 2796
Basic parts
Name Type Description Designer Length
BBa_K2593001 coding Hyaluronic acid synthase protein Hong Chen 1254
BBa_K2593003 coding Signal peptide Hong Chen 99
BBa_K2593004 coding Leech Hyaluronic acid hydrolase protein (58KDa) Huimin Li 1470
BBa_K2593009 coding Leech Hyaluronic acid hydrolase protein with an exported signal peptide and 6His-tag at N-terminus Huimin Li 1686
Composite parts
Name Type Description Designer Length
BBa_K2593006 composite Leech Hyaluronic acid hydrolase with an inducible promoter PlepA, RBS, an exporting signal peptide, 6Histag and a terminator. Yongtao Lin 1962
BBa_K2593007 composite Hyaluronic acid synthase protein with inducible promoter, RBS, and a terminator. Yongtao Lin 2672
BBa_K2593008 composite UDP-glucose dehydrogenase and UDP-gulcose pyrophosphoryase with a constitutive promotor, RBS and a terminator. Hong Chen 2796
Improved parts
In our project, we added to the characterization of  an existing Biobrick , design by Philip Hartz from iGEM Saarland 2014. Our P43-tuaD-gtaB-T1 composite part was designed based on two BioBrick parts ( BBa_K1469002 and BBa_K1469005 ) created by Saarland. For example, co-expression of tuaD-gtaB as an operon under the control a constitutive promoter so that recombinant B. Subtilis 168 produces elevated level of HA.

Copyright © 2018 SSTi-SZGD All Rights Reserved.
Copyright © 2018 SSTi-SZGD All Rights Reserved.