Difference between revisions of "Team:Fudan/Acknowledgement"
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| + | This html document is created by Tian Huang for Team Fudan iGEM 2018. | ||
| + | We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2. | ||
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| + | <title>2018 iGEM Team:Fudan - Acknowledgement</title> | ||
</head> | </head> | ||
<body> | <body> | ||
| − | |||
<!-- Fudan div at igem.org --> | <!-- Fudan div at igem.org --> | ||
<div id="FudanWrapper" class="white"> | <div id="FudanWrapper" class="white"> | ||
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<div class="nav-wrapper"> | <div class="nav-wrapper"> | ||
<div id="teamLogo" class="brand-logo"> | <div id="teamLogo" class="brand-logo"> | ||
| − | <a href="https://2018.igem.org/Team:Fudan" target="_self"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a> | + | <a href="https://2018.igem.org/Team:Fudan" target="_self"><img alt="2018 team Fudan logo" src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a> |
</div> | </div> | ||
| − | <ul id="nav-mobile" class="right"> | + | <ul id="nav-mobile" class="right orangeBorder"> |
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li> | ||
| − | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Dry | + | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Dry lab</a></li> |
| − | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Wet | + | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Wet lab</a></li> |
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Toolbox</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Toolbox</a></li> | ||
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Outreach</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Outreach</a></li> | ||
| Line 97: | Line 106: | ||
<!-- Dropdown and List elements in navigation bar --> | <!-- Dropdown and List elements in navigation bar --> | ||
<ul id="dropdown1" class="dropdown-content"> | <ul id="dropdown1" class="dropdown-content"> | ||
| − | <li><a href="">Demonstration</a></li> | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
</ul> | </ul> | ||
<ul id="dropdown2" class="dropdown-content"> | <ul id="dropdown2" class="dropdown-content"> | ||
| − | <li><a href="">Model</a></li> | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href="">Software</a></li> | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | ||
| + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> | ||
| + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> | ||
| + | <li><a href="/Team:Fudan/Software">Software</a></li> | ||
</ul> | </ul> | ||
<ul id="dropdown3" class="dropdown-content"> | <ul id="dropdown3" class="dropdown-content"> | ||
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href="">Safety</a></li> | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> | ||
| + | <li><a href="/Team:Fudan/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
<ul id="dropdown4" class="dropdown-content"> | <ul id="dropdown4" class="dropdown-content"> | ||
| − | <li><a href="">Basic | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href="">Composite | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href="">Parts | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href="">Measurement</a></li> | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> | ||
| + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> | ||
</ul> | </ul> | ||
<ul id="dropdown5" class="dropdown-content"> | <ul id="dropdown5" class="dropdown-content"> | ||
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> | ||
| + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> | ||
</ul> | </ul> | ||
<ul id="dropdown6" class="dropdown-content"> | <ul id="dropdown6" class="dropdown-content"> | ||
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href="">Attributions</a></li> | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| + | <li><a href="/Team:Fudan/Team">Members</a></li> | ||
| + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> | ||
</ul> | </ul> | ||
<!-- Slide-out navigator contents --> | <!-- Slide-out navigator contents --> | ||
<ul id="slide-out" class="sidenav"> | <ul id="slide-out" class="sidenav"> | ||
| − | <li><div class="sidenavBanner"> | + | <li style="padding: 0"><div class="sidenavBanner"> |
| − | <div class="background"> | + | <div class="background orangeBg"> |
</div> | </div> | ||
| − | <span class="white-text">Acknowledgement</span> | + | <p style="width: 100%;text-align: center;font-size: 24px"><span class="white-text">Acknowledgement</span></p> |
</div></li> | </div></li> | ||
<li> | <li> | ||
<ul class="collapsible expandable"> | <ul class="collapsible expandable"> | ||
| − | |||
<li> | <li> | ||
<div class="collapsible-header">Project</div> | <div class="collapsible-header">Project</div> | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href="">Demonstration</a></li> | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> | |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 151: | Line 171: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href="">Model</a></li> | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href="">Software</a></li> | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | ||
| + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> | ||
| + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> | ||
| + | <li><a href="/Team:Fudan/Software">Software</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 160: | Line 184: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href="">Safety</a></li> | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> | ||
| + | <li><a href="/Team:Fudan/Safety">Safety</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 170: | Line 196: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href="">Basic | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href="">Composite | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href="">Parts | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href="">Measurement</a></li> | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> | ||
| + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 181: | Line 209: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> | ||
| + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 191: | Line 221: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=""> | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href="">Attributions</a></li> | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href="" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| + | <li><a href="/Team:Fudan/Team">Members</a></li> | ||
| + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 205: | Line 237: | ||
<div id="pageContent" style=""> | <div id="pageContent" style=""> | ||
| − | <div id="contentBanner"> | + | <div id="contentBanner" class="figureBanner orangeBg"> |
<div class="row"> | <div class="row"> | ||
| − | <div class="col s12 m6 valign-wrapper"> | + | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> |
<h1>Acknowledgement</h1> | <h1>Acknowledgement</h1> | ||
</div> | </div> | ||
| − | <div class="col s12 m6 valign-wrapper"> | + | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> |
<span>Let us express the most sincere thanks to those | <span>Let us express the most sincere thanks to those | ||
who rendered their warm help to us. A successful | who rendered their warm help to us. A successful | ||
| Line 216: | Line 248: | ||
</div> | </div> | ||
</div> | </div> | ||
| − | < | + | <div id="figureBannerTitle" class="hide-on-small-only"> |
| − | <img src="../ | + | <h1 style="word-break: break-all;font-size: 50px;">Acknowledgement</h1> |
| + | <p><span>Let us express the most sincere thanks to those | ||
| + | who rendered their warm help to us. A successful | ||
| + | team can’t be constructed without them.</span></p> | ||
| + | </div> | ||
| + | <div class="hide-on-small-only"> | ||
| + | <img alt="2018 team Fudan title acknowledgement" src="https://static.igem.org/mediawiki/2018/1/1e/T--Fudan--title_ack.jpg"> | ||
| + | <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;"> | ||
| + | <defs> | ||
| + | <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraleft"> | ||
| + | <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="0%"/> | ||
| + | <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="100%"/> | ||
| + | </linearGradient> | ||
| + | </defs> | ||
| + | <g> | ||
| + | <rect id="svg_1" fill="url(#blackgraleft)" height="100%" width="100%"/> | ||
| + | </g> | ||
| + | </svg> | ||
| + | <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; right:0;top:0; width: 4%;height: 100%;"> | ||
| + | <defs> | ||
| + | <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraright"> | ||
| + | <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="0%"/> | ||
| + | <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="100%"/> | ||
| + | </linearGradient> | ||
| + | </defs> | ||
| + | <g> | ||
| + | <rect id="svg_2" fill="url(#blackgraright)" height="100%" width="100%"/> | ||
| + | </g> | ||
| + | </svg> | ||
| + | </div> | ||
</div> | </div> | ||
| + | |||
<!-- main content of the page --> | <!-- main content of the page --> | ||
<div class="container"> | <div class="container"> | ||
| − | <main style="margin:0"> | + | <main style="margin-right:0;"> |
| − | <div id="section1" class="section container | + | <div id="section1" class="section container"> |
| − | < | + | <h2>Acknowledgement</h2> |
<ul> | <ul> | ||
| − | <li> | + | <!--li> |
| − | </li><li> | + | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Zijie Yang</a> for his practical guidance in <a href="/protocols">molecular cloning</a>, |
| − | + | <a href="/primers">primers design</a>, <a href="/protocols">cell culture</a> and <a href="/measurements">flow data analysis</a>. | |
| − | and | + | He also helped us to start the <a href="/measurements">FACS</a> Jazz machine whenever we need. |
| − | + | </li--> | |
| − | + | <li> | |
| − | + | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yiheng Wang</a> for teaching us to model | |
| − | + | <a href="/model#1">DNA and transcription factor binding</a> using his previous <a href="https://2017.igem.org/Team:Fudan/Model" target="blank">model</a>. | |
| − | Yufang Zheng | + | </li> |
| − | + | <li> | |
| − | + | We thank <a href="https://2017.igem.org/Team:Fudan_China/Member" target="_blank">Tian Huang</a> for providing us with a <a href="https://materializecss.com/" target="_blank">Materialize</a> | |
| − | + | based wiki template and teaching us with basic knowledge about HTML and CSS. | |
| − | + | </li> | |
| − | + | <li> | |
| + | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yiming Cai</a> for giving us | ||
| + | advice on purifying EGFP protein, which was used in the beads assay at the early | ||
| + | stage of the project. | ||
| + | </li> | ||
| + | <li> | ||
| + | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yijie Pan</a> for giving us practical suggestions | ||
| + | on <a href="/Team:Fudan/Protocols" target="_blank">repeated fragment</a> molecular cloning and <a href="/Team:Fudan/Parts_Collection" target=_blank>BioBrick parts</a> documentation. | ||
| + | </li> | ||
| + | <li>We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Xinxuan Xiong</a> and <a href="https://2017.igem.org/Team:Fudan_China/Member" target="_blank">Xinyi Xu</a> | ||
| + | for giving us useful advice about the <a href="https://2017.igem.org/Team:Fudan/Bio-Art" target="_blank">Bio-ART</a> display and actively helping us in preparation for the display. | ||
| + | </li> | ||
| + | <li> | ||
| + | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Ao Feng</a> for helpful discussions on human practice. | ||
| + | </li><li>We thank Jingyi Hu for helping us taking bacteria on the morning, and playing an active role in some of our <a href="/Team:Fudan/Human_Practices" target="_blank">human practice</a>. | ||
| + | </li><li>We thank Haotian Guo for incisive comments on refining our presentation during and immediately after <a href="https://2018.igem.org/Team:ShanghaiTech/Collaborations" target="_blank">CCiC</a>. | ||
| + | </li><li>We thank Professor Xu Wei’s advice on performing flow cytometry and doing the flow data analysis. | ||
| + | </li><li>We thank Professor <a href="http://life.fudan.edu.cn/Data/View/1456" target="_blank">Yufang Zheng</a> for helpful discussions on the known Notch regulation and unpublished insight from her study with metalloprotease [ADAM10] and [ADAM17]. | ||
| + | </li><li>We thank Professor <a href="https://www.ki.si/en/departments/d12-department-of-synthetic-biology-and-immunology/" target=_blank>Roman Jerala</a> on email and face-to-face discussions on designing TALE based logic gates. | ||
| + | </li><li>We thank Professor <a href="https://blacklow.hms.harvard.edu/" target="_blank">Stephen Blacklow</a> on email and face-to-face discussions on engineered Notch receptor optimization. | ||
| + | </li><li>We thank Doctor Lixin Yang, Doctor Ganjun Yu, Doctor Xiaoying Bi, and Doctor Pengfei Luo for sparing precise time to have the discussion on <a href="/Team:Fudan/Human_Practices#section1" target="_blank">Interview doctors</a>. | ||
| + | </li><li>We thank Professor Xin Yuan, in the Department of Philosophy, for attending our Bio-Ethic debate and his comments enriched our understanding. | ||
| + | </li><li>We thank Professor <a href="http://life.fudan.edu.cn/Data/View/1457" target="_blank">Jiang Zhong</a>, Professor <a href="http://life.fudan.edu.cn/Data/View/1335" target="_blank">Xiaoming Ding</a>, Professor <a href="http://life.fudan.edu.cn/Data/View/1475" target="_blank">Qiang Huang</a>, and Professor <a href="http://life.fudan.edu.cn/Data/View/1391" target="_blank">Ting Ni</a> for giving us valuable suggestions and feeds at different stages of the project, from the initial brainstorm to the middle project progress and the pre-departure presentations. | ||
| + | </li><li>We thank the Club of FDU Bertalanffy Society for helping to rent the space for <a href="/Team:Fudan/Bio-Art" target="_blank">Bio-ART</a> display. | ||
| + | </li><li>We thank teachers in <a href="http://www.shedunews.com/jymp_qbdht.html" target="_blank">Qibao Dwight High School</a>, <a href="http://www.jincai.sh.cn/web/JCZX/273001.htm" target="_blank">Shanghai Jincai High School</a>, <a href="https://www.fdfz.cn/ " target="_blank">the High School Affiliated to Fudan University</a> for offering us with opportunities to implement <a href="/Team:Fudan/Human_Practices" target="_blank">our HP</a>. | ||
| + | </li><li>We thank <a href="https://2018.igem.org/Team:Fudan-CHINA" target="_blank">Team:Fudan_China</a> for teaching us how to use the plate reader. | ||
| + | </li> | ||
</ul> | </ul> | ||
| − | + | ||
| − | + | <h2>Photos</h2> | |
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<div id="acknowledgementPhoto"> | <div id="acknowledgementPhoto"> | ||
<h4>People</h4> | <h4>People</h4> | ||
| Line 283: | Line 335: | ||
<div class="row"> | <div class="row"> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/9/9f/T--Fudan--Xiaoying_Bi.jpg"> | + | <img alt="doctor Bi" src="https://static.igem.org/mediawiki/2018/9/9f/T--Fudan--Xiaoying_Bi.jpg"> |
<span>Xiaoying Bi</span> | <span>Xiaoying Bi</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/d/d7/T--Fudan--Stephen_Blacklow.jpg"> | + | <img alt="Blacklow S" src="https://static.igem.org/mediawiki/2018/d/d7/T--Fudan--Stephen_Blacklow.jpg"> |
<span>Stephen Blacklow</span> | <span>Stephen Blacklow</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/7/72/T--Fudan--Yiming_Cai.jpg"> | + | <img alt="Cai" src="https://static.igem.org/mediawiki/2018/7/72/T--Fudan--Yiming_Cai.jpg"> |
<span>Yiming Cai</span> | <span>Yiming Cai</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/4/4a/T--Fudan--Xiaoming_Ding.jpg"> | + | <img alt="Ding" src="https://static.igem.org/mediawiki/2018/4/4a/T--Fudan--Xiaoming_Ding.jpg"> |
<span>Xiaoming Ding</span> | <span>Xiaoming Ding</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/d/dc/T--Fudan--fengao.jpg"> | + | <img alt="Feng" src="https://static.igem.org/mediawiki/2018/d/dc/T--Fudan--fengao.jpg"> |
<span>Ao Feng</span> | <span>Ao Feng</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/5/5c/T--Fudan--Haotian_Guo.jpg"> | + | <img alt="Guo" src="https://static.igem.org/mediawiki/2018/5/5c/T--Fudan--Haotian_Guo.jpg"> |
<span>Haotian Guo</span> | <span>Haotian Guo</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/a/a6/T--Fudan--Jingyi_Hu.jpg"> | + | <img alt="Hu" src="https://static.igem.org/mediawiki/2018/a/a6/T--Fudan--Jingyi_Hu.jpg"> |
<span>Jingyi Hu</span> | <span>Jingyi Hu</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/c/c7/T--Fudan--Qiang_Huang.jpg"> | + | <img alt="Huang Q" src="https://static.igem.org/mediawiki/2018/c/c7/T--Fudan--Qiang_Huang.jpg"> |
<span>Qiang Huang</span> | <span>Qiang Huang</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/3/3d/T--Fudan--Tian_Huang.jpg"> | + | <img alt="Huang T" src="https://static.igem.org/mediawiki/2018/3/3d/T--Fudan--Tian_Huang.jpg"> |
<span>Tian Huang</span> | <span>Tian Huang</span> | ||
</div> | </div> | ||
| + | <div class="col s12 m3 l2"> | ||
| + | <img alt="Jerala" src="https://static.igem.org/mediawiki/2018/9/99/T--Fudan--LC--Roman_Jerala.jpg" /> | ||
| + | <span>Jerala Roman</span> | ||
| + | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img | + | <img alt="Luo" src="https://static.igem.org/mediawiki/2018/6/65/T--Fudan--Pengfei_Luo.jpg"> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<span>Pengfei Luo</span> | <span>Pengfei Luo</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/2/20/T--Fudan--Ting_Ni.jpg"> | + | <img alt="Ni" src="https://static.igem.org/mediawiki/2018/2/20/T--Fudan--Ting_Ni.jpg"> |
<span>Ting Ni</span> | <span>Ting Ni</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/3/3f/T--Fudan--Yijie_Pan.jpg"> | + | <img alt="Pan" src="https://static.igem.org/mediawiki/2018/3/3f/T--Fudan--Yijie_Pan.jpg"> |
<span>Yijie Pan</span> | <span>Yijie Pan</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/1/11/T--Fudan--Yiheng_Wang.jpg"> | + | <img alt="Wang" src="https://static.igem.org/mediawiki/2018/1/11/T--Fudan--Yiheng_Wang.jpg"> |
<span>Yiheng Wang</span> | <span>Yiheng Wang</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/8/80/T--Fudan--Xinxuan_Xiong.jpg"> | + | <img alt="Xiong" src="https://static.igem.org/mediawiki/2018/8/80/T--Fudan--Xinxuan_Xiong.jpg"> |
<span>Xinxuan Xiong</span> | <span>Xinxuan Xiong</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/1/16/T--Fudan--Wei_Xu.jpg"> | + | <img alt="Xu W" src="https://static.igem.org/mediawiki/2018/1/16/T--Fudan--Wei_Xu.jpg"> |
<span>Wei Xu</span> | <span>Wei Xu</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/a/a0/T--Fudan--Xinyi_Xu.jpg"> | + | <img alt="Xu X" src="https://static.igem.org/mediawiki/2018/a/a0/T--Fudan--Xinyi_Xu.jpg"> |
<span>Xinyi Xu</span> | <span>Xinyi Xu</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/4/4e/T--Fudan--Lixing_Yang.jpg"> | + | <img alt="doctor Yang L" src="https://static.igem.org/mediawiki/2018/4/4e/T--Fudan--Lixing_Yang.jpg"> |
<span>Lixing Yang</span> | <span>Lixing Yang</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/5/57/T--Fudan--Shunhua_Yang.jpg"> | + | <img alt="doctor Yang S" src="https://static.igem.org/mediawiki/2018/5/57/T--Fudan--Shunhua_Yang.jpg"> |
<span>Shunhua Yang</span> | <span>Shunhua Yang</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/6/6f/T--Fudan--Ganjun_Yu.jpg"> | + | <img alt="doctor Yu" src="https://static.igem.org/mediawiki/2018/6/6f/T--Fudan--Ganjun_Yu.jpg"> |
<span>Ganjun Yu</span> | <span>Ganjun Yu</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/6/6e/T--Fudan--Xin_Yuan.jpg"> | + | <img alt="doctor Yuan" src="https://static.igem.org/mediawiki/2018/6/6e/T--Fudan--Xin_Yuan.jpg"> |
<span>Xin Yuan</span> | <span>Xin Yuan</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/c/c6/T--Fudan--Jiang_Zhong.jpg"> | + | <img alt="Zhong" src="https://static.igem.org/mediawiki/2018/c/c6/T--Fudan--Jiang_Zhong.jpg"> |
<span>Jiang Zhong</span> | <span>Jiang Zhong</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/7/79/T--Fudan--Yufang_Zheng.jpg"> | + | <img alt="Zheng" src="https://static.igem.org/mediawiki/2018/7/79/T--Fudan--Yufang_Zheng.jpg"> |
<span>Yufang Zheng</span> | <span>Yufang Zheng</span> | ||
</div> | </div> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img | + | <img alt="Changhai" src="https://static.igem.org/mediawiki/2018/f/fd/T--Fudan--Changhai_Hospital.jpg"> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
<span>Changhai Hospital</span> | <span>Changhai Hospital</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/7/7f/T--Fudan--Jincai_High_School.png"> | + | <img alt="Jincai" src="https://static.igem.org/mediawiki/2018/7/7f/T--Fudan--Jincai_High_School.png"> |
<span>Jincai High School</span> | <span>Jincai High School</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/a/a7/T--Fudan--Fudanfuzhong.jpg"> | + | <img alt="Fuzhong" src="https://static.igem.org/mediawiki/2018/a/a7/T--Fudan--Fudanfuzhong.jpg"> |
<span>High School Affiliated to Fudan University</span> | <span>High School Affiliated to Fudan University</span> | ||
</div> | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/f/f1/T--Fudan--Qibao_Dwight.jpg"> | + | <img alt="Qibao" src="https://static.igem.org/mediawiki/2018/f/f1/T--Fudan--Qibao_Dwight.jpg"> |
<span>Shanghai Qibao Dwight High School</span> | <span>Shanghai Qibao Dwight High School</span> | ||
</div> | </div> | ||
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<div class="row"> | <div class="row"> | ||
<div class="col s12 m4 l3"> | <div class="col s12 m4 l3"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/6/65/T--Fudan--Fudan_China.jpg"> | + | <img alt="2018 Fudan-CHINA" src="https://static.igem.org/mediawiki/2018/6/65/T--Fudan--Fudan_China.jpg"> |
<span>Fudan-CHINA 2018</span> | <span>Fudan-CHINA 2018</span> | ||
</div> | </div> | ||
<div class="col s12 m4 l3"> | <div class="col s12 m4 l3"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/f/fa/T--Fudan--team_Fudan.png"> | + | <img alt="2017 Fudan" src="https://static.igem.org/mediawiki/2018/f/fa/T--Fudan--team_Fudan.png"> |
<span>Fudan 2017</span> | <span>Fudan 2017</span> | ||
</div> | </div> | ||
<div class="col s12 m4 l3"> | <div class="col s12 m4 l3"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/4/4f/T--Fudan--Team_Fudan_China2017.png"> | + | <img alt="2017 Fudan_China" src="https://static.igem.org/mediawiki/2018/4/4f/T--Fudan--Team_Fudan_China2017.png"> |
<span>Fudan_China 2017</span> | <span>Fudan_China 2017</span> | ||
</div> | </div> | ||
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<!--Abstract on content page--> | <!--Abstract on content page--> | ||
<div id="abstractContent" class="z-depth-2"> | <div id="abstractContent" class="z-depth-2"> | ||
| − | <a href="#!"><img src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> | + | <a href="#!"><img alt="2018 team Fudan abstract" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> |
<div class="container"> | <div class="container"> | ||
| − | < | + | <h2 style="margin: 0;padding: 10px 0;">Abstract</h2> |
| − | <p>Contact-dependent signaling is critical for multicellular biological | + | <p style="margin: 0">Contact-dependent signaling is critical for multicellular biological |
| − | + | events, yet customizing contact-dependent signal transduction between | |
| − | + | cells remains challenging. Here we have developed the ENABLE toolbox, a | |
| − | + | complete set of transmembrane binary logic gates. Each gate consists of | |
| − | + | 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic | |
| − | + | Notch receptors to enable cells to respond to different signals across the | |
| − | + | membrane reliably. These signals, individually amplified intracellularly by | |
| − | + | transcription, are further combined for computing. Our engineered zinc finger-based | |
| − | + | transcription factors perform binary computation and output designed products. | |
| − | + | In summary, we have combined spatially different signals in mammalian cells, | |
| − | + | and revealed new potentials for biological oscillators, tissue engineering, | |
| − | + | cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing | |
| − | + | contact-dependent signaling networks in mammals. The 3-layer design principle | |
| − | + | underlying ENABLE empowers any future development of transmembrane logic circuits, | |
| − | + | thus contributes a foundational advance to Synthetic Biology. | |
| + | </p> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | |||
| − | |||
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<i class="fa fa-sticky-note" style="font-size: 30px;line-height: 50px"></i> | <i class="fa fa-sticky-note" style="font-size: 30px;line-height: 50px"></i> | ||
</a> | </a> | ||
| − | <a href="# | + | <a href="#FudanWrapper" class="btn"> |
<i class="fa fa-angle-up" style="font-size: 48px;line-height: 45px"></i> | <i class="fa fa-angle-up" style="font-size: 48px;line-height: 45px"></i> | ||
</a> | </a> | ||
</div> | </div> | ||
| − | |||
<!-- Footer with sponsors and contact methods --> | <!-- Footer with sponsors and contact methods --> | ||
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<div class="container"> | <div class="container"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col | + | <div id="sponsor" class="col m3 s12 row"> |
| − | < | + | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img alt="2018 team Fudan logo" class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> |
| − | + | </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | |
| − | </ | + | </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> |
| − | + | </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png"> | |
| − | + | </a> | |
| − | + | <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size: 18px">ENABLE: making cells even smarter</h3> | |
</div> | </div> | ||
| − | <div class="col | + | <div id="usefulLinks" class="col m9 s12 row"> |
| − | <div class="col s12"> | + | <div class="col s12 l6 row"> |
| − | + | <div class="col s12 m4"> | |
| − | + | <span>Project</span> | |
| − | + | <ul> | |
| − | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> | |
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | </ul> | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> | ||
| + | <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class="col s12 m4"> | ||
| + | <span>Dry lab</span> | ||
| + | <ul> | ||
| + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> | ||
| + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> | ||
| + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | ||
| + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> | ||
| + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> | ||
| + | <li><a href="/Team:Fudan/Software">Software</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class="col s12 m4"> | ||
| + | <span>Wet lab</span> | ||
| + | <ul> | ||
| + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> | ||
| + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> | ||
| + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> | ||
| + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> | ||
| + | <li><a href="/Team:Fudan/Safety">Safety</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
</div> | </div> | ||
| − | <div class="col s12 | + | <div class="col s12 l6 row"> |
| − | < | + | <div class="col s12 m4"> |
| − | <li> | + | <span>Toolbox</span> |
| − | < | + | <ul> |
| − | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> | |
| − | </ul> | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> | ||
| + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> | ||
| + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> | ||
| + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class="col s12 m4"> | ||
| + | <span>Outreach</span> | ||
| + | <ul> | ||
| + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> | ||
| + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> | ||
| + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> | ||
| + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> | ||
| + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <div class="col s12 m4 active"> | ||
| + | <span>Team</span> | ||
| + | <ul> | ||
| + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> | ||
| + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> | ||
| + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> | ||
| + | <li><a href="/Team:Fudan/Team">Members</a></li> | ||
| + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> | ||
| + | </ul> | ||
| + | </div> | ||
</div> | </div> | ||
| − | |||
</div> | </div> | ||
</div> | </div> | ||
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<div class="footer-copyright"> | <div class="footer-copyright"> | ||
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| − | + | <div class="contactUS row"> | |
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Latest revision as of 15:06, 7 November 2018
- Addon: ribo
- Addon: TALE
- Addon: T2
- Model: transcriptional amplifer
- Model: Notch-ligand kinetics
- Software
Acknowledgement
Acknowledgement
- We thank Yiheng Wang for teaching us to model DNA and transcription factor binding using his previous model.
- We thank Tian Huang for providing us with a Materialize based wiki template and teaching us with basic knowledge about HTML and CSS.
- We thank Yiming Cai for giving us advice on purifying EGFP protein, which was used in the beads assay at the early stage of the project.
- We thank Yijie Pan for giving us practical suggestions on repeated fragment molecular cloning and BioBrick parts documentation.
- We thank Xinxuan Xiong and Xinyi Xu for giving us useful advice about the Bio-ART display and actively helping us in preparation for the display.
- We thank Ao Feng for helpful discussions on human practice.
- We thank Jingyi Hu for helping us taking bacteria on the morning, and playing an active role in some of our human practice.
- We thank Haotian Guo for incisive comments on refining our presentation during and immediately after CCiC.
- We thank Professor Xu Wei’s advice on performing flow cytometry and doing the flow data analysis.
- We thank Professor Yufang Zheng for helpful discussions on the known Notch regulation and unpublished insight from her study with metalloprotease [ADAM10] and [ADAM17].
- We thank Professor Roman Jerala on email and face-to-face discussions on designing TALE based logic gates.
- We thank Professor Stephen Blacklow on email and face-to-face discussions on engineered Notch receptor optimization.
- We thank Doctor Lixin Yang, Doctor Ganjun Yu, Doctor Xiaoying Bi, and Doctor Pengfei Luo for sparing precise time to have the discussion on Interview doctors.
- We thank Professor Xin Yuan, in the Department of Philosophy, for attending our Bio-Ethic debate and his comments enriched our understanding.
- We thank Professor Jiang Zhong, Professor Xiaoming Ding, Professor Qiang Huang, and Professor Ting Ni for giving us valuable suggestions and feeds at different stages of the project, from the initial brainstorm to the middle project progress and the pre-departure presentations.
- We thank the Club of FDU Bertalanffy Society for helping to rent the space for Bio-ART display.
- We thank teachers in Qibao Dwight High School, Shanghai Jincai High School, the High School Affiliated to Fudan University for offering us with opportunities to implement our HP.
- We thank Team:Fudan_China for teaching us how to use the plate reader.
Photos
People
Listed by alphabetical order of surnames.
Xiaoying Bi
Stephen Blacklow
Yiming Cai
Xiaoming Ding
Ao Feng
Haotian Guo
Jingyi Hu
Qiang Huang
Tian Huang
Jerala Roman
Pengfei Luo
Ting Ni
Yijie Pan
Yiheng Wang
Xinxuan Xiong
Wei Xu
Xinyi Xu
Lixing Yang
Shunhua Yang
Ganjun Yu
Xin Yuan
Jiang Zhong
Yufang Zheng
Labs, Organizations & Schools
Changhai Hospital
Jincai High School
High School Affiliated to Fudan University
Shanghai Qibao Dwight High School
iGEM Teams
Fudan-CHINA 2018
Fudan 2017
Fudan_China 2017
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.