Difference between revisions of "Team:Fudan/Acknowledgement"
m |
|||
| (9 intermediate revisions by 4 users not shown) | |||
| Line 5: | Line 5: | ||
We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2. | We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2. | ||
--> | --> | ||
| + | <!-- LC check on 2018-10-18 --> | ||
<head> | <head> | ||
<meta charset="UTF-8"> | <meta charset="UTF-8"> | ||
| Line 12: | Line 13: | ||
<!-- Font-awesome icons 4.7.0 --> | <!-- Font-awesome icons 4.7.0 --> | ||
| − | <link href="https://2018.igem.org/wiki/index.php?title=Template:Fudan/font-awesome.css&action=raw&ctype=text/css" rel="stylesheet"> | + | <link href="https://2018.igem.org/wiki/index.php?title=Template:Fudan/font-awesome.css&action=raw&ctype=text/css" rel="stylesheet" /> |
<!-- Materialize 1.0.0-rc.2 (Material Design like) --> | <!-- Materialize 1.0.0-rc.2 (Material Design like) --> | ||
| Line 18: | Line 19: | ||
<!-- Clear default CSS settings; CSS reset --> | <!-- Clear default CSS settings; CSS reset --> | ||
| − | <style | + | <style> |
*{margin: 0;padding: 0;list-style: none;} | *{margin: 0;padding: 0;list-style: none;} | ||
/* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */ | /* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */ | ||
| Line 70: | Line 71: | ||
} | } | ||
</style> | </style> | ||
| + | <title>2018 iGEM Team:Fudan - Acknowledgement</title> | ||
</head> | </head> | ||
| Line 104: | Line 106: | ||
<!-- Dropdown and List elements in navigation bar --> | <!-- Dropdown and List elements in navigation bar --> | ||
<ul id="dropdown1" class="dropdown-content"> | <ul id="dropdown1" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
</ul> | </ul> | ||
<ul id="dropdown2" class="dropdown-content"> | <ul id="dropdown2" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
<ul id="dropdown3" class="dropdown-content"> | <ul id="dropdown3" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
<ul id="dropdown4" class="dropdown-content"> | <ul id="dropdown4" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
<ul id="dropdown5" class="dropdown-content"> | <ul id="dropdown5" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
<ul id="dropdown6" class="dropdown-content"> | <ul id="dropdown6" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
| Line 159: | Line 161: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 169: | Line 171: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 182: | Line 184: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 194: | Line 196: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 207: | Line 209: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 219: | Line 221: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 282: | Line 284: | ||
<div class="container"> | <div class="container"> | ||
<main style="margin-right:0;"> | <main style="margin-right:0;"> | ||
| − | <div id="section1" class="section container | + | <div id="section1" class="section container"> |
<h2>Acknowledgement</h2> | <h2>Acknowledgement</h2> | ||
<ul> | <ul> | ||
| Line 292: | Line 294: | ||
<li> | <li> | ||
We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yiheng Wang</a> for teaching us to model | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yiheng Wang</a> for teaching us to model | ||
| − | <a href="/model#1">DNA and transcription factor binding | + | <a href="/model#1">DNA and transcription factor binding</a> using his previous <a href="https://2017.igem.org/Team:Fudan/Model" target="blank">model</a>. |
</li> | </li> | ||
<li> | <li> | ||
| Line 300: | Line 302: | ||
<li> | <li> | ||
We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yiming Cai</a> for giving us | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yiming Cai</a> for giving us | ||
| − | advice on purifying EGFP protein, which was used in | + | advice on purifying EGFP protein, which was used in the beads assay at the early |
stage of the project. | stage of the project. | ||
</li> | </li> | ||
<li> | <li> | ||
We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yijie Pan</a> for giving us practical suggestions | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Yijie Pan</a> for giving us practical suggestions | ||
| − | on <a href=" | + | on <a href="/Team:Fudan/Protocols" target="_blank">repeated fragment</a> molecular cloning and <a href="/Team:Fudan/Parts_Collection" target=_blank>BioBrick parts</a> documentation. |
</li> | </li> | ||
<li>We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Xinxuan Xiong</a> and <a href="https://2017.igem.org/Team:Fudan_China/Member" target="_blank">Xinyi Xu</a> | <li>We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Xinxuan Xiong</a> and <a href="https://2017.igem.org/Team:Fudan_China/Member" target="_blank">Xinyi Xu</a> | ||
| Line 312: | Line 314: | ||
<li> | <li> | ||
We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Ao Feng</a> for helpful discussions on human practice. | We thank <a href="https://2017.igem.org/Team:Fudan/Teamers" target="_blank">Ao Feng</a> for helpful discussions on human practice. | ||
| − | </li><li>We thank Jingyi Hu for helping us taking bacteria on the morning, and playing an active role in some of our <a href=" | + | </li><li>We thank Jingyi Hu for helping us taking bacteria on the morning, and playing an active role in some of our <a href="/Team:Fudan/Human_Practices" target="_blank">human practice</a>. |
| − | </li><li>We thank Haotian Guo for incisive comments on refining our presentation during and immediately after <a href="https://2018.igem.org/Team: | + | </li><li>We thank Haotian Guo for incisive comments on refining our presentation during and immediately after <a href="https://2018.igem.org/Team:ShanghaiTech/Collaborations" target="_blank">CCiC</a>. |
| − | </li><li>We thank Professor | + | </li><li>We thank Professor Xu Wei’s advice on performing flow cytometry and doing the flow data analysis. |
</li><li>We thank Professor <a href="http://life.fudan.edu.cn/Data/View/1456" target="_blank">Yufang Zheng</a> for helpful discussions on the known Notch regulation and unpublished insight from her study with metalloprotease [ADAM10] and [ADAM17]. | </li><li>We thank Professor <a href="http://life.fudan.edu.cn/Data/View/1456" target="_blank">Yufang Zheng</a> for helpful discussions on the known Notch regulation and unpublished insight from her study with metalloprotease [ADAM10] and [ADAM17]. | ||
| + | </li><li>We thank Professor <a href="https://www.ki.si/en/departments/d12-department-of-synthetic-biology-and-immunology/" target=_blank>Roman Jerala</a> on email and face-to-face discussions on designing TALE based logic gates. | ||
</li><li>We thank Professor <a href="https://blacklow.hms.harvard.edu/" target="_blank">Stephen Blacklow</a> on email and face-to-face discussions on engineered Notch receptor optimization. | </li><li>We thank Professor <a href="https://blacklow.hms.harvard.edu/" target="_blank">Stephen Blacklow</a> on email and face-to-face discussions on engineered Notch receptor optimization. | ||
| − | </li><li>We thank Doctor Lixin Yang, Doctor Ganjun Yu, Doctor Xiaoying Bi, and Doctor Pengfei Luo for sparing precise time to have the discussion on | + | </li><li>We thank Doctor Lixin Yang, Doctor Ganjun Yu, Doctor Xiaoying Bi, and Doctor Pengfei Luo for sparing precise time to have the discussion on <a href="/Team:Fudan/Human_Practices#section1" target="_blank">Interview doctors</a>. |
| − | </li><li>We thank Professor | + | </li><li>We thank Professor Xin Yuan, in the Department of Philosophy, for attending our Bio-Ethic debate and his comments enriched our understanding. |
| − | </li><li>We thank Professor <a href="http://life.fudan.edu.cn/Data/View/1457" target="_blank">Jiang Zhong</a>, Professor <a href="http://life.fudan.edu.cn/Data/View/1335" target="_blank">Xiaoming Ding</a>, Professor <a href="http://life.fudan.edu.cn/Data/View/1475" target="_blank">Qiang Huang</a>, and Professor <a href="http://life.fudan.edu.cn/Data/View/1391" target="_blank">Ting Ni</a> for giving us valuable suggestions and feeds at different stages of the project, from the initial brainstorm to the middle project progress and the pre-departure presentations. | + | </li><li>We thank Professor <a href="http://life.fudan.edu.cn/Data/View/1457" target="_blank">Jiang Zhong</a>, Professor <a href="http://life.fudan.edu.cn/Data/View/1335" target="_blank">Xiaoming Ding</a>, Professor <a href="http://life.fudan.edu.cn/Data/View/1475" target="_blank">Qiang Huang</a>, and Professor <a href="http://life.fudan.edu.cn/Data/View/1391" target="_blank">Ting Ni</a> for giving us valuable suggestions and feeds at different stages of the project, from the initial brainstorm to the middle project progress and the pre-departure presentations. |
| − | </li><li>We thank | + | </li><li>We thank the Club of FDU Bertalanffy Society for helping to rent the space for <a href="/Team:Fudan/Bio-Art" target="_blank">Bio-ART</a> display. |
| − | </li><li>We thank teachers in Qibao Dwight High School, Shanghai Jincai High School, the High School Affiliated to Fudan University for offering us with opportunities to implement | + | </li><li>We thank teachers in <a href="http://www.shedunews.com/jymp_qbdht.html" target="_blank">Qibao Dwight High School</a>, <a href="http://www.jincai.sh.cn/web/JCZX/273001.htm" target="_blank">Shanghai Jincai High School</a>, <a href="https://www.fdfz.cn/ " target="_blank">the High School Affiliated to Fudan University</a> for offering us with opportunities to implement <a href="/Team:Fudan/Human_Practices" target="_blank">our HP</a>. |
| − | </li><li>We thank | + | </li><li>We thank <a href="https://2018.igem.org/Team:Fudan-CHINA" target="_blank">Team:Fudan_China</a> for teaching us how to use the plate reader. |
</li> | </li> | ||
</ul> | </ul> | ||
| Line 367: | Line 370: | ||
<span>Tian Huang</span> | <span>Tian Huang</span> | ||
</div> | </div> | ||
| + | <div class="col s12 m3 l2"> | ||
| + | <img alt="Jerala" src="https://static.igem.org/mediawiki/2018/9/99/T--Fudan--LC--Roman_Jerala.jpg" /> | ||
| + | <span>Jerala Roman</span> | ||
| + | </div> | ||
<div class="col s12 m3 l2"> | <div class="col s12 m3 l2"> | ||
<img alt="Luo" src="https://static.igem.org/mediawiki/2018/6/65/T--Fudan--Pengfei_Luo.jpg"> | <img alt="Luo" src="https://static.igem.org/mediawiki/2018/6/65/T--Fudan--Pengfei_Luo.jpg"> | ||
| Line 510: | Line 517: | ||
<span>Project</span> | <span>Project</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | + | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
<li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | ||
</ul> | </ul> | ||
| Line 520: | Line 527: | ||
<span>Dry lab</span> | <span>Dry lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 531: | Line 538: | ||
<span>Wet lab</span> | <span>Wet lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 543: | Line 550: | ||
<span>Toolbox</span> | <span>Toolbox</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 554: | Line 561: | ||
<span>Outreach</span> | <span>Outreach</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 564: | Line 571: | ||
<span>Team</span> | <span>Team</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 577: | Line 584: | ||
<div class="footer-copyright"> | <div class="footer-copyright"> | ||
<div class="container"> | <div class="container"> | ||
| − | + | <div class="contactUS row"> | |
| − | + | <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg E301, 2005 Songhu Rd, Shanghai | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn | |
| − | + | </div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
</div> | </div> | ||
</footer> | </footer> | ||
Latest revision as of 15:06, 7 November 2018
- Addon: ribo
- Addon: TALE
- Addon: T2
- Model: transcriptional amplifer
- Model: Notch-ligand kinetics
- Software
Acknowledgement
Acknowledgement
- We thank Yiheng Wang for teaching us to model DNA and transcription factor binding using his previous model.
- We thank Tian Huang for providing us with a Materialize based wiki template and teaching us with basic knowledge about HTML and CSS.
- We thank Yiming Cai for giving us advice on purifying EGFP protein, which was used in the beads assay at the early stage of the project.
- We thank Yijie Pan for giving us practical suggestions on repeated fragment molecular cloning and BioBrick parts documentation.
- We thank Xinxuan Xiong and Xinyi Xu for giving us useful advice about the Bio-ART display and actively helping us in preparation for the display.
- We thank Ao Feng for helpful discussions on human practice.
- We thank Jingyi Hu for helping us taking bacteria on the morning, and playing an active role in some of our human practice.
- We thank Haotian Guo for incisive comments on refining our presentation during and immediately after CCiC.
- We thank Professor Xu Wei’s advice on performing flow cytometry and doing the flow data analysis.
- We thank Professor Yufang Zheng for helpful discussions on the known Notch regulation and unpublished insight from her study with metalloprotease [ADAM10] and [ADAM17].
- We thank Professor Roman Jerala on email and face-to-face discussions on designing TALE based logic gates.
- We thank Professor Stephen Blacklow on email and face-to-face discussions on engineered Notch receptor optimization.
- We thank Doctor Lixin Yang, Doctor Ganjun Yu, Doctor Xiaoying Bi, and Doctor Pengfei Luo for sparing precise time to have the discussion on Interview doctors.
- We thank Professor Xin Yuan, in the Department of Philosophy, for attending our Bio-Ethic debate and his comments enriched our understanding.
- We thank Professor Jiang Zhong, Professor Xiaoming Ding, Professor Qiang Huang, and Professor Ting Ni for giving us valuable suggestions and feeds at different stages of the project, from the initial brainstorm to the middle project progress and the pre-departure presentations.
- We thank the Club of FDU Bertalanffy Society for helping to rent the space for Bio-ART display.
- We thank teachers in Qibao Dwight High School, Shanghai Jincai High School, the High School Affiliated to Fudan University for offering us with opportunities to implement our HP.
- We thank Team:Fudan_China for teaching us how to use the plate reader.
Photos
People
Listed by alphabetical order of surnames.
Xiaoying Bi
Stephen Blacklow
Yiming Cai
Xiaoming Ding
Ao Feng
Haotian Guo
Jingyi Hu
Qiang Huang
Tian Huang
Jerala Roman
Pengfei Luo
Ting Ni
Yijie Pan
Yiheng Wang
Xinxuan Xiong
Wei Xu
Xinyi Xu
Lixing Yang
Shunhua Yang
Ganjun Yu
Xin Yuan
Jiang Zhong
Yufang Zheng
Labs, Organizations & Schools
Changhai Hospital
Jincai High School
High School Affiliated to Fudan University
Shanghai Qibao Dwight High School
iGEM Teams
Fudan-CHINA 2018
Fudan 2017
Fudan_China 2017
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.