Difference between revisions of "Team:Fudan/Notebook"
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</style> | </style> | ||
| + | <title>2018 iGEM Team:Fudan - Notebook</title> | ||
</head> | </head> | ||
<body> | <body> | ||
| − | |||
<!-- Fudan div at igem.org --> | <!-- Fudan div at igem.org --> | ||
<div id="FudanWrapper" class="white"> | <div id="FudanWrapper" class="white"> | ||
| − | <div id="FudanBody" class="white | + | <div id="FudanBody" class="white"> |
<header> | <header> | ||
<!-- empty bar --> | <!-- empty bar --> | ||
| Line 86: | Line 72: | ||
<div class="nav-wrapper"> | <div class="nav-wrapper"> | ||
<div id="teamLogo" class="brand-logo"> | <div id="teamLogo" class="brand-logo"> | ||
| − | <a href="https://2018.igem.org/Team:Fudan" target="_self"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a> | + | <a href="https://2018.igem.org/Team:Fudan" target="_self"><img alt="2018 team Fudan logo" src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a> |
</div> | </div> | ||
| − | <ul id="nav-mobile" class="right | + | <ul id="nav-mobile" class="right blueBorder"> |
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown1">Project</a></li> | ||
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Dry lab</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown2">Dry lab</a></li> | ||
| − | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown3">Wet lab</a></li> | + | <li class="hide-on-med-and-down"><a class="dropdown-trigger thisPageLink" data-target="dropdown3">Wet lab</a></li> |
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Toolbox</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown4">Toolbox</a></li> | ||
<li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Outreach</a></li> | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown5">Outreach</a></li> | ||
| − | <li class="hide-on-med-and-down"><a class="dropdown-trigger | + | <li class="hide-on-med-and-down"><a class="dropdown-trigger" data-target="dropdown6">Team</a></li> |
<li> | <li> | ||
<a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> | <a id="navList" data-target="slide-out" class="waves-effect waves-light sidenav-trigger right"> | ||
| Line 106: | Line 92: | ||
<!-- Dropdown and List elements in navigation bar --> | <!-- Dropdown and List elements in navigation bar --> | ||
<ul id="dropdown1" class="dropdown-content"> | <ul id="dropdown1" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> | ||
</ul> | </ul> | ||
<ul id="dropdown2" class="dropdown-content"> | <ul id="dropdown2" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
<ul id="dropdown3" class="dropdown-content"> | <ul id="dropdown3" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
<ul id="dropdown4" class="dropdown-content"> | <ul id="dropdown4" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
<ul id="dropdown5" class="dropdown-content"> | <ul id="dropdown5" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
<ul id="dropdown6" class="dropdown-content"> | <ul id="dropdown6" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
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<ul id="slide-out" class="sidenav"> | <ul id="slide-out" class="sidenav"> | ||
<li style="padding: 0"><div class="sidenavBanner"> | <li style="padding: 0"><div class="sidenavBanner"> | ||
| − | <div class="background | + | <div class="background"> |
</div> | </div> | ||
| − | <p style="width: 100%;text-align: center;font-size: 24px"><span class="white-text"> | + | <p style="width: 100%;text-align: center;font-size: 24px"><span class="white-text">Our notebook</span></p> |
</div></li> | </div></li> | ||
<li> | <li> | ||
| Line 162: | Line 149: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | + | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
| Line 172: | Line 160: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> | |
| − | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> | |
| − | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 185: | Line 173: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 197: | Line 185: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 210: | Line 198: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 222: | Line 210: | ||
<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div id="pageContent" style=""> | <div id="pageContent" style=""> | ||
| − | |||
<div id="contentBanner" class="figureBanner"> | <div id="contentBanner" class="figureBanner"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col s12 hide-on-med-and-up"> | + | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> |
<h1>Our notebook</h1> | <h1>Our notebook</h1> | ||
</div> | </div> | ||
| − | <div class="col s12 hide-on-med-and-up"> | + | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> |
| − | <span> | + | <span>Here we present a main outline of our progress throughout the year.</span> |
</div> | </div> | ||
</div> | </div> | ||
<div id="figureBannerTitle" class="hide-on-small-only"> | <div id="figureBannerTitle" class="hide-on-small-only"> | ||
<h1>Our notebook</h1> | <h1>Our notebook</h1> | ||
| − | <p><span> | + | <p><span>Here we present a main outline of our progress throughout the year.</span></p> |
</div> | </div> | ||
<div class="hide-on-small-only"> | <div class="hide-on-small-only"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/b/ba/T--Fudan--title_notebook.jpg"> |
<svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;"> | <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;"> | ||
<defs> | <defs> | ||
| Line 279: | Line 266: | ||
</div> | </div> | ||
| − | <!-- | + | <!-- main content of the page --> |
<div class="container"> | <div class="container"> | ||
<!-- side navigator of page content --> | <!-- side navigator of page content --> | ||
| − | <ul id="pageContentNav" class="hide-on-med-and-down z-depth-0"> | + | <!--<ul id="pageContentNav" class="hide-on-med-and-down z-depth-0"> |
| − | < | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | </ul> | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
| − | <main> | + | |
| + | <li class="onThisPageNav"><a href="#section1">General safety concerns</a></li> | ||
| + | <li class="onThisPageNav"><a href="#section2">Laboratory practice</a></li> | ||
| + | <li class="onThisPageNav"><a href="#section3">Specific safety concerns</a></li> | ||
| + | </ul>--> | ||
| + | <main style="margin:0"> | ||
<div class="section container"> | <div class="section container"> | ||
| − | < | + | <h2>Monthly Highlights</h2> |
| − | < | + | <h3>April</h3> |
| − | < | + | <ul> |
| − | + | <li>First functional SynNotch constructed | |
| − | </ | + | </li><li>Developed a simple testification method of SynNotch—shaking beads linked with antigen |
| − | </ | + | </li><li>Transcription factor and promotor pairs constructed and tested</li> |
| + | </ul> | ||
| + | <h3>May</h3> | ||
| + | <ul> | ||
| + | <li>SynNotch leakage problem detection | ||
| + | </li><li>Successfully constructed and tested the first mutated version of SynNotch | ||
| + | </li><li>Started constructing composite promoters for logic gates</li> | ||
| + | </ul> | ||
| + | <h3>June</h3> | ||
| + | <ul> | ||
| + | <li>SynNotch with various extra- and intra-cellular domain construction | ||
| + | </li><li>SynNotch and composite promoter verification in cell</li> | ||
| + | </ul> | ||
| + | <h3>August</h3> | ||
| + | <ul> | ||
| + | <li>Batch construction of plasmids used in “amplifier” and “combiner” layers for all sixteen logic | ||
| + | </li><li>Continual construction and testification of various mutation versions of SynNotch</li> | ||
| + | </ul> | ||
| + | <h3>September</h3> | ||
| + | <ul> | ||
| + | <li>Successfully constructed double-stable cell line of two SynNotch receptors | ||
| + | </li><li>Finished constructing plasmids used in logic gates</li> | ||
| + | </ul> | ||
| + | <h3>October</h3> | ||
| + | <ul> | ||
| + | <li>Parts construction | ||
| + | </li><li>All sixteen logic testification in cell</li> | ||
| + | </ul> | ||
| + | <ul class="collapsible expandable"> | ||
| + | <li style="list-style-type: none"> | ||
| + | <div class="collapsible-header">Click to see details</div> | ||
| + | <div class="collapsible-body"> | ||
| + | <embed src="https://static.igem.org/mediawiki/2018/3/32/T--Fudan--Notebook.pdf" width="100%" height="800px"> | ||
| + | </div> | ||
| + | </li> | ||
| + | </ul> | ||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
| − | |||
</main> | </main> | ||
</div> | </div> | ||
| Line 307: | Line 330: | ||
<!--Abstract on content page--> | <!--Abstract on content page--> | ||
<div id="abstractContent" class="z-depth-2"> | <div id="abstractContent" class="z-depth-2"> | ||
| − | <a href="#!"><img src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> | + | <a href="#!"><img alt="2018 team Fudan abstract" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> |
<div class="container"> | <div class="container"> | ||
<h2 style="margin: 0;padding: 10px 0;">Abstract</h2> | <h2 style="margin: 0;padding: 10px 0;">Abstract</h2> | ||
| − | <p style="margin:0 | + | <p style="margin: 0">Contact-dependent signaling is critical for multicellular biological |
events, yet customizing contact-dependent signal transduction between | events, yet customizing contact-dependent signal transduction between | ||
cells remains challenging. Here we have developed the ENABLE toolbox, a | cells remains challenging. Here we have developed the ENABLE toolbox, a | ||
| Line 327: | Line 350: | ||
</p> | </p> | ||
</div> | </div> | ||
| + | </div> | ||
| + | |||
| + | <!-- Floating Btns --> | ||
| + | <div class="floatingBtn"> | ||
| + | <a href="#!" id="abstractBtn" class="btn"> | ||
| + | <i class="fa fa-sticky-note" style="font-size: 30px;line-height: 50px"></i> | ||
| + | </a> | ||
| + | <a href="#FudanWrapper" class="btn"> | ||
| + | <i class="fa fa-angle-up" style="font-size: 48px;line-height: 45px"></i> | ||
| + | </a> | ||
</div> | </div> | ||
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<div class="row"> | <div class="row"> | ||
<div id="sponsor" class="col m3 s12 row"> | <div id="sponsor" class="col m3 s12 row"> | ||
| − | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> | + | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> |
</a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | ||
</a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | ||
| Line 346: | Line 379: | ||
<span>Project</span> | <span>Project</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | + | ||
| − | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
<li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | ||
</ul> | </ul> | ||
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<span>Dry lab</span> | <span>Dry lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> | |
| − | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="col s12 m4"> | + | <div class="col s12 m4 active"> |
<span>Wet lab</span> | <span>Wet lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
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<span>Toolbox</span> | <span>Toolbox</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<span>Outreach</span> | <span>Outreach</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="col s12 m4 | + | <div class="col s12 m4"> |
<span>Team</span> | <span>Team</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="footer-copyright"> | <div class="footer-copyright"> | ||
<div class="container"> | <div class="container"> | ||
| − | + | <div class="contactUS row"> | |
| − | + | <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg E301, 2005 Songhu Rd, Shanghai | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn | |
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Latest revision as of 15:18, 7 November 2018
- Addon: ribo
- Addon: TALE
- Addon: T2
- Model: transcriptional amplifer
- Model: Notch-ligand kinetics
- Software
Our notebook
Monthly Highlights
April
- First functional SynNotch constructed
- Developed a simple testification method of SynNotch—shaking beads linked with antigen
- Transcription factor and promotor pairs constructed and tested
May
- SynNotch leakage problem detection
- Successfully constructed and tested the first mutated version of SynNotch
- Started constructing composite promoters for logic gates
June
- SynNotch with various extra- and intra-cellular domain construction
- SynNotch and composite promoter verification in cell
August
- Batch construction of plasmids used in “amplifier” and “combiner” layers for all sixteen logic
- Continual construction and testification of various mutation versions of SynNotch
September
- Successfully constructed double-stable cell line of two SynNotch receptors
- Finished constructing plasmids used in logic gates
October
- Parts construction
- All sixteen logic testification in cell
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.