Difference between revisions of "Team:Fudan/Primers"
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| + | <title>2018 iGEM Team:Fudan - Primers</title> | ||
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> | ||
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<ul id="dropdown2" class="dropdown-content"> | <ul id="dropdown2" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
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<ul id="dropdown3" class="dropdown-content"> | <ul id="dropdown3" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
<ul id="dropdown5" class="dropdown-content"> | <ul id="dropdown5" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
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<ul id="slide-out" class="sidenav"> | <ul id="slide-out" class="sidenav"> | ||
<li style="padding: 0"><div class="sidenavBanner"> | <li style="padding: 0"><div class="sidenavBanner"> | ||
| − | <div class="background | + | <div class="background"> |
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| − | <p style="width: 100%;text-align: center;font-size: 24px"><span class="white-text"> | + | <p style="width: 100%;text-align: center;font-size: 24px"><span class="white-text">Primers used</span></p> |
</div></li> | </div></li> | ||
<li> | <li> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | + | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> | ||
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> | |
| − | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> | |
| − | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div id="pageContent" style=""> | <div id="pageContent" style=""> | ||
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<div id="contentBanner" class="figureBanner"> | <div id="contentBanner" class="figureBanner"> | ||
<div class="row"> | <div class="row"> | ||
| − | <div class="col s12 hide-on-med-and-up"> | + | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> |
<h1>Primers used</h1> | <h1>Primers used</h1> | ||
</div> | </div> | ||
| − | <div class="col s12 hide-on-med-and-up"> | + | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> |
| − | <span> | + | <span>Here we present a library of the vast collection of primers we have designed</span> |
</div> | </div> | ||
</div> | </div> | ||
<div id="figureBannerTitle" class="hide-on-small-only"> | <div id="figureBannerTitle" class="hide-on-small-only"> | ||
<h1>Primers used</h1> | <h1>Primers used</h1> | ||
| − | <p><span> | + | <p><span>Here we present a library of the vast collection of primers we have designed</span></p> |
</div> | </div> | ||
<div class="hide-on-small-only"> | <div class="hide-on-small-only"> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/3/30/T--Fudan--title_primer.jpg"> |
<svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;"> | <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; left:0;top:0; width: 4%;height: 100%;"> | ||
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<!-- @@@@ main content of the page --> | <!-- @@@@ main content of the page --> | ||
<div class="container"> | <div class="container"> | ||
| − | < | + | <main style="margin:0"> |
| − | + | <div class="section container"> | |
| − | + | <h2>Primers</h2> | |
| − | + | <div class="tableHolder" style="overflow:hidden"> | |
| − | + | <table> | |
| − | + | <tr><th>#</th><th>Oligo Name</th><th>DNA Sequence (5 to 3)</th></tr> | |
| − | + | <tr><td>O1</td><td>pLL7.1-F(S)</td><td>GGTCTATATAAGCAGAGCTGG</td></tr> | |
| − | + | <tr><td>O2</td><td>pLL7.1-R(S)</td><td>AACCGGAACCCTTAAACATG</td></tr> | |
| − | + | <tr><td>O3</td><td>LaG16-F(G_pLL7.1)</td><td>TGAACCGTCAGATCCGCTAGAATTCGCCACCATGGCCCTGCCCGTGACC</td></tr> | |
| − | + | <tr><td>O4</td><td>LaG16-R(G_mNc)</td><td>AGTCCAGGATGCTCACGGTCACCTGGGTG</td></tr> | |
| + | <tr><td>O5</td><td>mNc-F(G_LaG16)</td><td>GACCGTGAGCATCCTGGACTACAGCTTCACAGGTGGCGC</td></tr> | ||
| + | <tr><td>O6</td><td>mNc|e-R(G_tTa)</td><td>ATCTAGACATCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O7</td><td>tTa-F(G_mNc|e)</td><td>GCGCCGGCGGATGTCTAGATTAGATAAAAGTAAAGTGATTAAC</td></tr> | ||
| + | <tr><td>O8</td><td>tTa-R(G_pLL7.1)</td><td>CCCTCGACGAATTGCCTGCAGGCTACCCACCGTACTCGTCAATTC</td></tr> | ||
| + | <tr><td>O9</td><td>LaG16-R(G-mNc(e))</td><td>CCACACAGGGGCTCACGGTCACCTGGGTG</td></tr> | ||
| + | <tr><td>O10</td><td>mNc(e)-F(G_LaG16)</td><td>GACCGTGAGCCCCTGTGTGGGTAGCAACCCCTGCTAC</td></tr> | ||
| + | <tr><td>O11</td><td>mNotch 1 core-PF</td><td>ATCCTGGACTACAGCTTCACAG</td></tr> | ||
| + | <tr><td>O12</td><td>mNotch 1 core (extended)-PF</td><td>CCCTGTGTGGGTAGCAACC</td></tr> | ||
| + | <tr><td>O13</td><td>mNotch 1 core-PB</td><td>CCGCCGGCGCTTG</td></tr> | ||
| + | <tr><td>O14</td><td>LaG16-F(EcoRI-Kozark)</td><td>GCTGAATTCGCCACCATGGCCCTGCCCGTG</td></tr> | ||
| + | <tr><td>O15</td><td>LaG16-B(OE mNotch) </td><td>CTGTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGG</td></tr> | ||
| + | <tr><td>O16</td><td>LaG16-B(OE mNCe) </td><td>GGTTGCTACCCACACAGGGGCTCACGGTCACCTGGG</td></tr> | ||
| + | <tr><td>O17</td><td>mcherry-F(OE mNotch) </td><td>CAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O18</td><td>mCherry-B(UAA-HindIII)</td><td>GCTAAGCTTTTACTTGTACAGCTCGTCCATGC</td></tr> | ||
| + | <tr><td>O19</td><td>CMV-F(ala-EcoRI-ala-XbaI) </td><td>GCAGAATTCGCATCTAGAGACATTGATTATTGACGAGTTATTAATAGTAATCAATTACG</td></tr> | ||
| + | <tr><td>O20</td><td>LacOi-F(OE intron)</td><td>CGTAATTGTGAGCGGATAACAATTACGTTCGCCCCGTGCCCCG </td></tr> | ||
| + | <tr><td>O21</td><td>LacOi-B(OE intron)</td><td>CGTAATTGTTATCCGCTCACAATTACGGGCAACGCAGCGACTC </td></tr> | ||
| + | <tr><td>O22</td><td>intron-B(SpeI-ala-HindIII-ala)</td><td>GCAAAGCTTGCAACTAGTCTGTAGGAAAAAGAAGAAGGCATGAAC </td></tr> | ||
| + | <tr><td>O23</td><td>LaG16-F(G_pML2-EcoRI-Kozark)</td><td>AGTTATTAGGTCCCTCGACGAATTCGCCACCATGGCCCTGCCCGTGACC</td></tr> | ||
| + | <tr><td>O24</td><td>LaG16-R(G_mNC)</td><td>GTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGGTG</td></tr> | ||
| + | <tr><td>O25</td><td>LaG16-R(G_mNCe)</td><td>GGGTTGCTACCCACACAGGGGCTCACGGTCACCTGGGTG</td></tr> | ||
| + | <tr><td>O26</td><td>mcherry-F(G_mNC)</td><td>TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O27</td><td>mcherry-R(HindIII G_pML2)</td><td>AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG</td></tr> | ||
| + | <!-- tr><td>O28</td><td>rev_41BB_CD3z9</td><td>序列缺失</td></tr --> | ||
| + | <tr><td>O29</td><td>hNotch-4168</td><td>CCCTGCCTGGGCGGCAAC</td></tr> | ||
| + | <tr><td>O30</td><td>5286-hNotch</td><td>CCGCCGGCGCTTGCGGG</td></tr> | ||
| + | <tr><td>O31</td><td>tTA-start</td><td>CAAGCGCCGGCGG ATGTCTAGATTAGATAAAAGTAAAGTG </td></tr> | ||
| + | <tr><td>O32</td><td>rev_tTA-end-3NotI-2-Hind3-5</td><td>GTTCCAAGCTTAAGCGCTACCCACCGTACTCG</td></tr> | ||
| + | <tr><td>O33</td><td>EGFP_BglⅡ_F</td><td>GCAGATCTatggtgagcaagggcga</td></tr> | ||
| + | <tr><td>O34</td><td>EGFP_XbalI_R</td><td>GCTCTAGActttacttgtacagctcgtccat</td></tr> | ||
| + | <tr><td>O35</td><td>rtTA_pLL7.0_F</td><td>TGAACCGTCAGATCCGCTAGAAGTTTGTACAAAAAAGCAG</td></tr> | ||
| + | <tr><td>O36</td><td>rtTA_EGFP_R</td><td>TGGCACTTTTTTTAAATGATTTGCCCTCCC</td></tr> | ||
| + | <tr><td>O37</td><td>rtTa_EGFP_F</td><td>GCAAATCATTTAAAAAAAGTGCCACCTGACGTC</td></tr> | ||
| + | <tr><td>O38</td><td>LacI_pLL7.0_R</td><td> GCAAATCATTTAAAAAAAGTGCCACCTGACGTC</td></tr> | ||
| + | <tr><td>O39</td><td>lacI_mFF4_ployA_F</td><td>TACCGCGGGCCCGGGATCCATTTGACAATTAAAGCTTGAGG</td></tr> | ||
| + | <tr><td>O40</td><td>mFF4_ployA_pCAGop_R</td><td>attaataactaGCGCAGCGAGTCAGTGAG</td></tr> | ||
| + | <tr><td>O41</td><td>mFF4_polyA_pCAGop_F</td><td>ctcgctgcgcTAGTTATTAATAGTAATCAATTACGGG</td></tr> | ||
| + | <tr><td>O42</td><td>pCAGop_pLL7.0_R</td><td>CTTGCTCACCATGGTGGCGACTGTAGGAAAAAGAAGAAGG</td></tr> | ||
| + | <tr><td>O43</td><td>mFF4_pll7.0_R</td><td>CTTGCTCACCATGGTGGCGAGCGCAGCGAGTCAGTGAG</td></tr> | ||
| + | <tr><td>O44</td><td>TRE_pLL7.0_R</td><td>cgcggtaccgtcgactgcagTACCGAGCTCGAAATCTC</td></tr> | ||
| + | <tr><td>O45</td><td>TRE_GAL4_F</td><td>TACCGCGGGCCCGGGATCCAAAGTTTGTACAAAAAAGCAG</td></tr> | ||
| + | <tr><td>O46</td><td>GAL4_UAS5_R</td><td>TCACATGAGATACAGTCAACTGTCTTTG</td></tr> | ||
| + | <tr><td>O47</td><td>GAS4_UAS5_F</td><td>gttgactgtaTCTCATGTGATTACGCCAAG</td></tr> | ||
| + | <tr><td>O48</td><td>UAS5_mCherry_R</td><td>cttgctcaccatggtggcgaTGCTTTTTTGTACAAACTTGG</td></tr> | ||
| + | <tr><td>O49</td><td>pLL7.0_mCherry_F</td><td>aagcttatcgataatcaacctctggattacaaaatttgtgaaaga</td></tr> | ||
| + | <tr><td>O50</td><td>pLL7.0_mCherry_R</td><td>GATATCGAATTGTACCTAGTGGAACCGGAACC</td></tr> | ||
| + | <tr><td>O51</td><td>mCherry_FF4_polyA_F</td><td>actaggtacaattcgatatcGAATTCTGCAGTCGACGG</td></tr> | ||
| + | <tr><td>O52</td><td>FF4_ployA_pLL7.0_R</td><td>ggttgattatcgataagcttTTTGCCCTCCCATATGTC</td></tr> | ||
| + | <tr><td>O53</td><td>EGFPpoly(A)F</td><td>agtttggttagtaccgcgctGCAAATCATTTAAAAAAAGTGCCACC</td></tr> | ||
| + | <tr><td>O54</td><td>EGFPpoly(A)R</td><td>cctggagaatgccaccATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O55</td><td>Bi_TRE F</td><td>tgctcaccatggtggcATTCTCCAGGCGATCTGACGG</td></tr> | ||
| + | <tr><td>O56</td><td>Bi_TRE R</td><td>cgcggtaccgtcgactgcaggaattcCGCGGTACCGTCGACTGC</td></tr> | ||
| + | <tr><td>O57</td><td>pll7.0.1 F</td><td>cagggatattcaccattatcgtttcagacc</td></tr> | ||
| + | <tr><td>O58</td><td>poly(A)F</td><td>cgacggtaccgcgggcccggAACTTGTTTATTGCAGCTTATAATG</td></tr> | ||
| + | <tr><td>O59</td><td>poly(A)R</td><td>caccatggtggcgaccggtgGCAGTGAAAAAAATGCTTTATTTG</td></tr> | ||
| + | <tr><td>O60</td><td>BiTRE F</td><td> ccatggtggcattctccaggGCCTCAGGAGATCTcAATTCtcc</td></tr> | ||
| + | <tr><td>O61</td><td>BiTRE R</td><td> cgcggtaccgtcgactgcagaattctaccgagctcgaaatctcca</td></tr> | ||
| + | <tr><td>O62</td><td> pSB1C3-VR</td><td> ATTACCGCCTTTGAGTGAGC</td></tr> | ||
| + | <tr><td>O63</td><td> pSB1C3-VF</td><td> TGCCACCTGACGTCTAAGAA</td></tr> | ||
| + | <tr><td>O64</td><td>pSB1C3-BP </td><td> GAATTCGCGGCCGCTTC</td></tr> | ||
| + | <tr><td>O65</td><td>pSB1C3-BS</td><td> CTGCAGCGGCCGCTAC</td></tr> | ||
| + | <tr><td>O66</td><td>EGFP-F(G_pQM6.2) </td><td>gcaagagcaagggccaattgGTGAGCAAGGGCGAGGAG</td></tr> | ||
| + | <tr><td>O67</td><td>EGFP-R(G_P2A) </td><td>gtcgacccCTTGTACAGCTCGTCCATGC</td></tr> | ||
| + | <tr><td>O68</td><td>P2A-F(G_EGFP) </td><td>ctgtacaagGGGTCGACGGGCTCCGGC</td></tr> | ||
| + | <tr><td>O69</td><td>mcherry-R(G_pQM6.2)</td><td>ccctttttctggagacaagcttTTACTTGTACAGCTCGTCCATGCCG</td></tr> | ||
| + | <tr><td>O70</td><td>pQM6.2-R(L-P2A) </td><td>ggatctggggccggggttctcc</td></tr> | ||
| + | <tr><td>O71</td><td>pQM6.2-F(L-EGFP)</td><td>atggtgagcaagggcgaggagc</td></tr> | ||
| + | <tr><td>O72</td><td>alpha-SP-LaG16-F(3-AgeI)</td><td>GCAACCGGTGCCACCATGGCCCTGC</td></tr> | ||
| + | <tr><td>O73</td><td>tTa-R(HindIII-3) </td><td>GCAAAGCTTCTACCCACCGTACTCGTCAATTCc</td></tr> | ||
| + | <tr><td>O74</td><td>P-minCMV-F</td><td>GAATTCGCGGCCGCTTCTAGgaggtaggcgtgtacggtg</td></tr> | ||
| + | <tr><td>O75</td><td>S-minCMV-R</td><td>CTGCAGCGGCCGCTACTAGTAggcgatctgacggttcactaaac</td></tr> | ||
| + | <tr><td>O76</td><td>P-pSV40-F</td><td>GAATTCGCGGCCGCTTCTAGgtgtgtcagttagggtgtgg</td></tr> | ||
| + | <tr><td>O77</td><td>S-pSV40-R</td><td>CTGCAGCGGCCGCTACTAGTAtttgcaaaagcctaggcctccaaaaaag</td></tr> | ||
| − | + | <tr><td>O82</td><td>SV40NLS-KRAB-F</td><td>CCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg</td></tr> | |
| + | <tr><td>O83</td><td>BS-F</td><td>tactagtagcggccgctgc</td></tr> | ||
| + | <tr><td>O84</td><td>BP-R</td><td>ctctagaagcggccgcg</td></tr> | ||
| + | <tr><td>O85</td><td>KRAB-R </td><td>caccagccagggttcctc</td></tr> | ||
| + | <tr><td>O86</td><td>mcherry-F </td><td>ATGGTGAGCAAGGGCGAGGAGGATAACATG</td></tr> | ||
| + | <tr><td>O87</td><td>pTight-F(S) </td><td>AGCTCGTTTAGTGAACCGTCAG</td></tr> | ||
| + | <tr><td>O88</td><td>3 LTR (Delta-U3)-R(S)</td><td>GCGTTACTTAAGCTAGCTTGCC</td></tr> | ||
| + | <tr><td>O89</td><td>P2A-F(S) </td><td>ACCAACTTCTCCCTGCTGAAG</td></tr> | ||
| + | <tr><td>O90</td><td>mcherry-R(S) </td><td>CATGTGCACCTTGAAGCGC</td></tr> | ||
| + | <tr><td>O91</td><td>BP protect</td><td>tttctggaattcgcggccg</td></tr> | ||
| + | <tr><td>O92</td><td>BS protect</td><td>gccggactgcagcg</td></tr> | ||
| + | <tr><td>O93</td><td>2* ZF 42-10 operator</td><td>GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAC GCT GCT c TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O94</td><td>2* ZF 54-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O95</td><td>2* ZF 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a GAG TGA GGA c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O96</td><td>2* ZF 21-16 operator</td><td>GAATTCGCGGCCGCTTCTAG a TTA GAA GTG a a TTA GAA GTG a TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O97</td><td>1* ZF 42-10 & 54-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O98</td><td>1* ZF 42-10 & 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O99</td><td>1* ZF 54-8 & 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O100</td><td>2* ZFHD1</td><td>GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG</td></tr> | ||
| + | <tr><td>O101</td><td>1*PIR</td><td style="word-break: break-all">GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O102</td><td>pCMV-F(S) </td><td>cgtgtacggtgggaggtctatataag</td></tr> | ||
| + | <tr><td>O103</td><td>pML2-R(S) </td><td>acgagattccagcaggtcg</td></tr> | ||
| + | <tr><td>O106</td><td>pSB1C3-BP(L)</td><td>tttctggaattcgcggccgcttc</td></tr> | ||
| + | <tr><td>O107</td><td>pSB1C3-BS(L)</td><td>gccggactgcagcggccgc</td></tr> | ||
| + | <tr><td>O108</td><td>PIR-F(6-XbaI)</td><td>GCCGCTTCTAGagGAAATAGCGCTGTACAGCG</td></tr> | ||
| + | <tr><td>O109</td><td>2*ZFHD1-F(6-XbaI)</td><td>GCCGCTTCTAGagTAATGATGGGCGTATAATGATGG</td></tr> | ||
| + | <tr><td>O110</td><td>CD8-alpha-SP-F(G_pML2)</td><td>gatccgctagcgctaccggtgccaccATGGCCTTACCAGTGACCGCCTTG</td></tr> | ||
| + | <tr><td>O111</td><td>alpha-GPC3-R(G_mNce)</td><td>acacagggTGGTCGCGGCGCTGGCGT</td></tr> | ||
| + | <tr><td>O112</td><td>mNce-F(G_alpha-GPC3)</td><td>cgcgaccaCCCTGTGTGGGTAGCAACC</td></tr> | ||
| + | <tr><td>O113</td><td>tTa-R(G_pML2) </td><td>gttatattaagggttccaagcttCTACCCACCGTACTCGTCAATTC</td></tr> | ||
| + | <tr><td>O114</td><td>alpha-GPC3-R(G_mNc) </td><td>TCCAGGATTGGTCGCGGCGCTGGCGT</td></tr> | ||
| + | <tr><td>O115</td><td>mNc-F(G_alpha-GPC3) </td><td>cgcgaccaATCCTGGACTACAGCTTCACAG</td></tr> | ||
| + | <tr><td>O116</td><td>CD8-alpha-F2(G_pML2)</td><td>GATCCGCTAGCGCTACCGGTGCCACCATGGCCCTGCCCGTGACC</td></tr> | ||
| + | <tr><td>O117</td><td>CD8-alpha-SP-F(G_pML2)</td><td>cgtcagatccgctagcgctaccggtGCCACCATGGCCCTGCCCG</td></tr> | ||
| + | <tr><td>O118</td><td>KRAB-R(G_pML2) </td><td>aagttatattaagggttccaagctttcaCACCAGCCAGGGTTCCTC</td></tr> | ||
| + | <tr><td>O119</td><td>mNc|e-R(G_Gal4DBD) </td><td>gcagcttcatCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O120</td><td>Gal4DBD-F(G_mNc|e) </td><td>gcgccggcggATGAAGCTGCTGAGCAGCATC</td></tr> | ||
| + | <tr><td>O121</td><td>Gal4DBD-R(G_SV40NLS)</td><td>tacctcctcctccCACTGTCAGCTGCCGCTG</td></tr> | ||
| + | <tr><td>O122</td><td>SV40NLS-F(G_Gal4DBD)</td><td>gctgacagtgGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O123</td><td>mNc|e-R(G_PIP) </td><td>cccttgacatCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O124</td><td>PIP-F(G_mNc|e) </td><td>gcgccggcggATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O125</td><td>PIP-R(G_SV40NLS) </td><td>tacctcctcctccGGCCTGCTCCACCATAGC</td></tr> | ||
| + | <tr><td>O126</td><td>SV40NLS-F(G_PIP) </td><td>ggagcaggccGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O127</td><td>mNc|e-R(G_ZF21-16) </td><td>tagggctagcCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O128</td><td>ZF21-16-F(G_mNc|e) </td><td>gcgccggcggGCTAGCCCTAGAGTGAGAACAGG</td></tr> | ||
| + | <tr><td>O129</td><td>ZF21-16-R(G_SV40NLS)</td><td>tacctcctcctccGGATCCCTTCTCGCCTGTG</td></tr> | ||
| + | <tr><td>O130</td><td>SV40NLS-F(G_ZF21-16)</td><td>gaagggatccGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O131</td><td>mNc|e-R(G_ZF42-10) </td><td>tagggctagcCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O132</td><td>ZF42-10-F(G_mNc|e) </td><td>gcgccggcggGCTAGCCCTAGAGTGAGAACAG</td></tr> | ||
| + | <tr><td>O133</td><td>ZF42-10-R(G_SV40NLS)</td><td>tacctcctcctccGGATCCCTTTTCTCCGGTG</td></tr> | ||
| + | <tr><td>O134</td><td>SV40NLS-F(G_ZF42-10)</td><td>aaagggatccGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O135</td><td>mNc|e-R(G_ZF43-8) </td><td>tggggctagcCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O136</td><td>ZF43-8-F(G_mNc|e) </td><td>gcgccggcggGCTAGCCCCAGAGTGAGAAC</td></tr> | ||
| + | <tr><td>O137</td><td>ZF43-8-R(G_SV40NLS) </td><td>tacctcctcctccGGATCCTTTTTCACCTGTATGTG</td></tr> | ||
| + | <tr><td>O138</td><td>SV40NLS-F(G_ZF43-8) </td><td>aaaaggatccGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O139</td><td>mNc|e-R(G_ZF54-8) </td><td>tgggagaagcCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O140</td><td>ZF54-8-F(G_mNc|e) </td><td>gcgccggcggGCTTCTCCCAGAGTAAGAACGGG</td></tr> | ||
| + | <tr><td>O141</td><td>ZF54-8-R(G_SV40NLS) </td><td>tacctcctcctccGGACCCTTTTTCGCCGGTG</td></tr> | ||
| + | <tr><td>O142</td><td>SV40NLS-F(G_ZF54-8) </td><td>aaaagggtccGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O143</td><td>mNc|e-R(G_ZFHD1) </td><td>atgggcgttcCCGCCGGCGCTTGCGGGA</td></tr> | ||
| + | <tr><td>O144</td><td>ZFHD1-F(G_mNc|e) </td><td>gcgccggcggGAACGCCCATATGCTTGC</td></tr> | ||
| + | <tr><td>O145</td><td>ZFHD1-R(G_SV40NLS) </td><td>tacctcctcctccGTTGATTCTTTTTTCTTTCTGGC</td></tr> | ||
| + | <tr><td>O146</td><td>SV40NLS-F(G_ZFHD1) </td><td>aagaatcaacGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O147</td><td>EcoRV-mcherry-F(KOD)</td><td>GATATCATGGTGAGCAAGGGCGAGGAGG</td></tr> | ||
| + | <tr><td>O148</td><td>P2A-F(6-AgeI-EcoRI)</td><td>GCAGCAACCGGTGGTGGAGAATTCggAtcAggTgccaccaacttctccctgctgaag</td></tr> | ||
| + | <tr><td>O149</td><td>EGFP-R(HindIII-6) </td><td>GCAGCAAAGCTTTTActtgtacagctcgtccatgccga</td></tr> | ||
| + | <tr><td>O150</td><td>KRAB-R(G_P2A)</td><td>AGTTGGTGGCACCTGATCCGAATTCCACCAGCCAGGGTTCCTCGC</td></tr> | ||
| + | <tr><td>O151</td><td>KRAB-F(GGGGS-SV40NLS)</td><td>GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg</td></tr> | ||
| + | <tr><td>O160</td><td>lacIF</td><td>accccggccccagatccgatATGAAACCAGTAACGTTATAC</td></tr> | ||
| + | <tr><td>O161</td><td>LacIR</td><td>ggtaccgtcgAACCTTCCTCTTCTTCTTAG</td></tr> | ||
| + | <tr><td>O162</td><td>SV40F</td><td>gaggaaggttCGACGGTACCGCGGGCCC</td></tr> | ||
| + | <tr><td>O163</td><td>sv40R</td><td>attaataactaCACCATGGTGGCGACCGGTG</td></tr> | ||
| + | <tr><td>O164</td><td>pCAGF</td><td>caccatggtgTAGTTATTAATAGTAATCAATTACGGG</td></tr> | ||
| + | <tr><td>O165</td><td>pCAGR</td><td>tcgcccttgctcaccatgatCTGTAGGAAAAAGAAGAAGG</td></tr> | ||
| + | <tr><td>O166</td><td>tTa-R(EcoRI-6)</td><td>GCAGCAGAATTCCTACCCACCGTACTCGTCAATTCC</td></tr> | ||
| + | <tr><td>O167</td><td>tTa-R(EcoRI-6 f)</td><td>GCAGCAGAATTCCCCACCGTACTCGTCAATTCC</td></tr> | ||
| + | <tr><td>O168</td><td>EGFP-R(S)</td><td>gctgaacttgtggccgtttac</td></tr> | ||
| + | <tr><td>O169</td><td>mcherry-R(S)</td><td>TGATGATGGCCATGTTATCCTCC</td></tr> | ||
| + | <tr><td>O170</td><td>PIR/ZFD F</td><td>tttctggaattcgcggccgcttcTAGag</td></tr> | ||
| + | <tr><td>O171</td><td>pSBIC3 F</td><td>Ggcagaatttcagataaaaaaaatccttagctttcgctaaggatg</td></tr> | ||
| + | |||
| + | <tr><td>O172</td><td>pSBIC3 R</td><td>gcagggtggtgacaccttgcccttttttg</td></tr> | ||
| + | <tr><td>O173</td><td>NPminCMV</td><td>GAATTCGCGGCCGCTTCTAgagaggtaggcgtgtacggtg</td></tr> | ||
| + | <tr><td>O174</td><td>P2A-R(G_EcoRV-mcherry)</td><td>TCGGATCTGGGGCCGGGGTTCTCCTCCACGTCGC</td></tr> | ||
| + | <tr><td>O175</td><td>mcherry-F(G_P2A-EcoRV)</td><td>ccggccccAGATCCGATATCATGGTGAGCAAG</td></tr> | ||
| + | <tr><td>O176</td><td>SV40poly(A)-F</td><td>AACTTGTTTATTGCAGCTTATAATGG</td></tr> | ||
| + | <tr><td>O177</td><td>SV40poly(A)-R1</td><td>TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTG</td></tr> | ||
| + | <tr><td>O178</td><td>SV40poly(A)-R2</td><td>GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACC</td></tr> | ||
| + | <tr><td>O179</td><td>rtTA-F-(AgeI)</td><td>tttctgACCGGTATGTCTAGATTAGATAAAAGTAAAGTGATTAACAGCG</td></tr> | ||
| + | <tr><td>O180</td><td>P2A-R(G2_mcherry)</td><td>ccatggtggcgatatcGGGGCCGGGGTTCTCCTC</td></tr> | ||
| + | <tr><td>O181</td><td>mcherry-F(G2_P2A)</td><td>ccggccccgatatcgccaccATGGTGAGCAAGGGCGAGGagg</td></tr> | ||
| + | <tr><td>O182</td><td>Kaba_B_R</td><td>taaacaagttctaCACCAGCCAGGGTTCCTC</td></tr> | ||
| + | <tr><td>O183</td><td>LPA_F</td><td>gctggtgtagAACTTGTTTATTGCAGCTTATAATG</td></tr> | ||
| + | <tr><td>O184</td><td>LPA_R</td><td>catccttagcGATCCAGACATGATAAGATAC</td></tr> | ||
| + | <tr><td>O185</td><td>Prom_F</td><td>tgtctggatcGCTAAGGATGATTTCTGGAATTCGCGGC</td></tr> | ||
| + | <tr><td>O186</td><td>Prom_R</td><td>ttgctcaccatggtggcgatTTGCCGGACTGCAGCGGC</td></tr> | ||
| + | <tr><td>O187</td><td>TF1_F(same with tf4)</td><td>aggagaaccccggccccgatGCTAGCCCTAGAGTGAGAAC</td></tr> | ||
| + | <tr><td>O188</td><td>TF2_F</td><td>aggagaaccccggccccgatGCTAGCCCCAGAGTGAGAAC</td></tr> | ||
| + | <tr><td>O189</td><td>TF3_F</td><td>aggagaaccccggccccgatGCTTCTCCCAGAGTAAGAACG</td></tr> | ||
| + | <tr><td>O190</td><td>TF8_F</td><td>aggagaaccccggccccgatGAACGCCCATATGCTTGC</td></tr> | ||
| + | <tr><td>O191</td><td>TF9_F</td><td>aggagaaccccggccccgatATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O192</td><td>lacI_F</td><td>aggagaaccccggccccgatATGAAACCAGTAACGTTATAC</td></tr> | ||
| + | <tr><td>O193</td><td>LacI_R</td><td>taaacaagttctaAACCTTCCTCTTCTTCTTAG</td></tr> | ||
| + | <tr><td>O194</td><td>lacI_40_F</td><td>gaggaaggtttagAACTTGTTTATTGCAGCTTATAATG</td></tr> | ||
| + | <tr><td>O195</td><td>LacI_40_R</td><td>attaataactaGATCCAGACATGATAAGATAC</td></tr> | ||
| + | <tr><td>O196</td><td>pCAGop_F</td><td>tgtctggatcTAGTTATTAATAGTAATCAATTACGGG</td></tr> | ||
| + | <tr><td>O197</td><td>pCAGop_R</td><td>ttgctcaccatggtggcgatCTGTAGGAAAAAGAAGAAGG</td></tr> | ||
| + | <tr><td>O198</td><td>VP64-F(GGGGS-SV40NLS)</td><td>GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAGACGCGCTGGACGatt</td></tr> | ||
| + | <tr><td>O199</td><td>VP64-R </td><td>TAACATATCGAGATCGAAATCGTCCAGag</td></tr> | ||
| + | <tr><td>O200</td><td>VP64-F2(GSSSS-SV40NLS)</td><td>GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAgatgctttagacgattttgactt</td></tr> | ||
| + | <tr><td>O201</td><td>VP64-R2 </td><td>tagcatatctagatcaaagtcatctagg</td></tr> | ||
| + | <tr><td>O202</td><td>rtTAA-F(6-AgeI) </td><td>GCAGCAACCGGTatgtctagactggacaagagcaaag</td></tr> | ||
| + | <tr><td>O203</td><td>rtTAA-R(HindIII-6)</td><td>TGCTGCAAGCTTttacccggggagcatgtcaag</td></tr> | ||
| + | <tr><td>O204</td><td>gal_F</td><td>aggagaaccccggccccgatATGAAGCTGCTGAGCAGC</td></tr> | ||
| + | <tr><td>O205</td><td>Gal_poly(a)_R</td><td>acatgagatcGATCCAGACATGATAAGATAC</td></tr> | ||
| + | <tr><td>O206</td><td>Uas_F</td><td>tgtctggatcGATCTCATGTGATTACGCC</td></tr> | ||
| + | <tr><td>O207</td><td>Uas_R</td><td>ttgctcaccatggtggcgatTGTACAAACTTGGGTCGAATTG</td></tr> | ||
| + | <tr><td>O208</td><td>PGK-F(6-EcoRI-6-BamHI-6-MfeI)</td><td>GCAGCAGAATTCGGTGGAGGATCCGGAGGTCAATTGgggtaggggaggcgc</td></tr> | ||
| + | <tr><td>O209</td><td>PuroR-R(NotI-6) </td><td>TGCTGCGCGGCCGCtcaggcaccgggcttg</td></tr> | ||
| + | <tr><td>O210</td><td>EGFP-R(NotI-6-HindIII6)</td><td>TGCTGCAAGCTTTGCAGCGCGGCCGCTTActtgtacagctcgtccatgccgag</td></tr> | ||
| + | <tr><td>O211</td><td>rtTAA-F(6-EcoRI)</td><td>GCAGCAGAATTCatgtctagactggacaagagcaaag</td></tr> | ||
| + | <tr><td>O212</td><td>rtTAA-R(BamHI-6)</td><td>TGCAGCGGATCCttacccggggagcatgtcaag</td></tr> | ||
| + | <tr><td>O213</td><td>Gal4DBD-F</td><td>ATGAAGCTGCTGAGCAGCatc</td></tr> | ||
| + | <tr><td>O214</td><td>PIP-F</td><td>ATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O215</td><td>ZF21-16|24-10-F</td><td>GCTAGCCCTAGAGTGAGAACAGG</td></tr> | ||
| + | <tr><td>O216</td><td>ZF43-8-F</td><td>GCTAGCCCCAGAGTGAGAAC</td></tr> | ||
| + | <tr><td>O217</td><td>ZF54-8</td><td>GCTTCTCCCAGAGTAAGAACGG</td></tr> | ||
| + | <tr><td>O218</td><td>ZFHD1-F</td><td>GAACGCCCATATGCTTGCC</td></tr> | ||
| + | <tr><td>O219</td><td>M13fwdL</td><td>gtgtaaaacgacggccagtg</td></tr> | ||
| + | <tr><td>O220</td><td>M13revL</td><td>gctatggacaggaaacagctatgac</td></tr> | ||
| + | <tr><td>O221</td><td>G_puro_F</td><td>tgacatgctccccgggtaaaGCAGCAGAATTCGGTGGAGGATC</td></tr> | ||
| + | <tr><td>O222</td><td>G_puro_R</td><td>aagttatattaagggttccaTCAGGCACCGGGCTTGCG</td></tr> | ||
| + | <tr><td>O223</td><td>G_pCAGop_R</td><td>ttgctcaccatggtggcgatATGAGACAGCACAACAAC</td></tr> | ||
| + | <tr><td>O224</td><td>PGK-R(S)</td><td>gtgtagcgccaagtgcc</td></tr> | ||
| + | <tr><td>O225</td><td>Ne-R(KOD)</td><td>CCCTGATTGTAGCAGGGGTTGCTAC</td></tr> | ||
| + | <tr><td>O226</td><td>Ne-F(KOD)</td><td>CACCTGTGAGCCCACATCCGAGAAC</td></tr> | ||
| + | <tr><td>O227</td><td>M13revL2</td><td>AATTTCACACAGGAAACAGCTATGAC</td></tr> | ||
| + | <tr><td>O228</td><td>EGFP-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCatggtgagcaagggcgag</td></tr> | ||
| + | <tr><td>O229</td><td>mCherry-F(6-Kozak-MfeI)</td><td>GCAGCAICAATTGGCCACCATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O230</td><td>PGK-F(6-EcoRI-6-BamHI-6-SalI)</td><td>GGAGGTGAATTCGGTGGAGGATCCGGAGGTGTCGACgggtaggggaggcgc</td></tr> | ||
| + | <tr><td>O231</td><td>PGK-R(MfeI-6)</td><td>GCAGCACAATTGcgaaaggcccggagatgag</td></tr> | ||
| + | <tr><td>O232</td><td>mCherry-R(NotI-6-HindIII-6)</td><td>GGTGGTAAGCTTGGTGGAGCGGCCGCTTACTTGTACAGCTCGTCCATGCC</td></tr> | ||
| + | <tr><td>O233</td><td>Gal4DBD-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCATGAAGCTGCTGAGCAGCatc</td></tr> | ||
| + | <tr><td>O234</td><td>PIP-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O235</td><td>ZF21-16|42-10-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCatgGCAAGCCCTAGAGTGAGAACAGgttc</td></tr> | ||
| + | <tr><td>O236</td><td>ZF43-8-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCatgGCAAGCCCCAGAGTGAGAACTGGTTCTAAGACACCTCC</td></tr> | ||
| + | <tr><td>O237</td><td>ZF54-8-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCatgGCTTCTCCCAGAGTAAGAACGG</td></tr> | ||
| + | <tr><td>O238</td><td>ZFHD1-F(6-MfeI-Kozak)</td><td>GCAGCACAATTGGCCACCATGGAACGCCCATATGCTTGCc</td></tr> | ||
| + | <tr><td>O239</td><td>KRAB-R(TAA-HindIII-6)</td><td>GCAGCAAAGCTTTTACACCAGCCAGGGTTCCTC</td></tr> | ||
| + | <tr><td>O240</td><td>VP64-R(TAA-HindIII-6)</td><td>GCAGCAAAGCTTTTAtagcatatctagatcaaagtcatctagggcatc</td></tr> | ||
| + | <tr><td>O241</td><td>P2A-R(MfeI-6-HindIII-6)</td><td>TCCTCCAAGCTTTCCTTACAATTGGGGGCCGGGGTTCTC</td></tr> | ||
| + | <tr><td>O242</td><td>EGFP|mCherry-F(6-AgeI-6-EcoRI-Kozak)</td><td>GGAGGAACCGGTGGAGGTGAATTCGCCACCATGGTGAGCAAGGGCGAGGAG</td></tr> | ||
| + | <tr><td>O243</td><td>EGFP|mCherry-F(6-BamHI-AgeI)</td><td>GCAGCAGGATCCACCGGTgccaccatggtgagcaagggcgag</td></tr> | ||
| + | <tr><td>O244</td><td>L.Lanier loxp seq</td><td>tggtgggtacctagtggaacc</td></tr> | ||
| + | <tr><td>O245</td><td>pSB1C3-BS(L)(AgeI-6)</td><td>GCAGCAACCGGTgccggactgcagcggccgc</td></tr> | ||
| + | <tr><td>O246</td><td>EGFP-F(L_CD8-alpha-SP1)</td><td>tgctgctgcccctggccctgctgctgcacgccgccagaccTatggtgagcaagggcgag</td></tr> | ||
| + | <tr><td>O247</td><td>EGFP-F(L_CD8-alpha-SP2)</td><td>ATGgccctgcccgtgaccgccctgctgctgcccctgg</td></tr> | ||
| + | <tr><td>O248</td><td>CD8-alpha-SP-F(6-AgeI)</td><td>GCAGCAACCGGTGCCACCATGgccctgcccgt</td></tr> | ||
| + | <tr><td>O249</td><td>EGFP-R(OE_CD86tm) </td><td>gtgaagataaaagccgcttgtacagctcgtccatgc</td></tr> | ||
| + | <tr><td>O250</td><td>CD86tm-F(OE-EGFP) </td><td>ggacgagctgtacaagcggcttttatcttcacctttctct</td></tr> | ||
| + | <tr><td>O251</td><td>CD86-R(HindIII-6) </td><td>GCAGCAAAGCTTTCAatcccgaggccgcttc</td></tr> | ||
| + | <tr><td>O252</td><td>CD86tm-F(L)</td><td>cggcttttatcttcacctttctctatagagcttgaggaccctcagc</td></tr> | ||
| + | <tr><td>O253</td><td>uas-t-R</td><td>gagatgatccagacatgataagatacattg</td></tr> | ||
| + | <tr><td>O254</td><td>uas-t-F</td><td>cttctggatccaattcgacccaag</td></tr> | ||
| + | <tr><td>O255</td><td>Sv40-UAS-F</td><td>tatcatgtctggatcatctcGTGTGTCAGTTAGGGTGTG</td></tr> | ||
| + | <tr><td>O256</td><td>SV40-UAS-R</td><td>tggcgtaatcTTTGCAAAAGCCTAGGCC</td></tr> | ||
| + | <tr><td>O257</td><td>Gal4BDF</td><td>cttttgcaaaGATTACGCCAAGCTACGG</td></tr> | ||
| + | <tr><td>O258</td><td>Gal4BDR</td><td>ggtcgaattggatccagaagTATCTAGAGTCTCCGCTC</td></tr> | ||
| + | <tr><td>O259</td><td>mCherry-F(6-Kozak-MfeI)</td><td>GCAGCACAATTGGCCACCATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O260</td><td>UAS-pre</td><td>ttctggAATTCGCGGCCGCTTCTAGaggatcatctcgtgtgtcagtta</td></tr> | ||
| + | <tr><td>O261</td><td>UAS-suf</td><td>gccggacTGCAGCGGCCGCTACTAGTAagaaggtaactagagtctccgctcg</td></tr> | ||
| + | <tr><td>O262</td><td>LaG16-F(L_CD8-alpha-SP1)</td><td>CGCCAGACCTGAGCAAAAGTTGATCTCAGAGGAGGACCTCATGACCATGGCCCAGGTG</td></tr> | ||
| + | <tr><td>O263</td><td>P2A-R(L_CD8-alpha-SP1)</td><td>GCAGGGCCAGGGGCAGCAGCAGGGCGGTCACGGGCAGGGCCATGGGGCCGGGGTTCTC</td></tr> | ||
| + | <tr><td>O264</td><td>P2A-R(L_CD8-alpha-SP2)</td><td>CTCCTCTGAGATCAACTTTTGCTCAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC</td></tr> | ||
| + | <tr><td>O265</td><td>P2A-R(L_CD8-alpha-SP2-HA) </td><td>GTAATCTGGCACGTCATAGGGGTAAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC</td></tr> | ||
| + | <tr><td>O266</td><td>alpha-GPC3-F(L_CD8-alpha-SP1)</td><td>CTTACCCCTATGACGTGCCAGATTACGCGGATGTTGTGATGACTCAGTCTCCACTCTC</td></tr> | ||
| + | <tr><td>O267</td><td>LaG16-F(L_CD8-alpha-SP2) </td><td>GACCGCCCTGCTGCTGCCCCTGGCCCTGCTGCTGCACGCCGCCAGACCTGAGCAAAAG</td></tr> | ||
| + | <tr><td>O268</td><td>CD8-alpha-SP-F(6-AgeI-Kozak)</td><td>GCAGCAACCGGTGCCACCATGGCCCTGCCCGTGACCGCCCTGCTGCTGCCCC </td></tr> | ||
| + | <tr><td>O269</td><td>VP64-R(EcoRI-6 f)</td><td>GCAGCAGAATTCtagcatatccagatcaaagtcatctagggcatcgg </td></tr> | ||
| + | <tr><td>O270</td><td>pUAS-F(6-BP)</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGcatgtgattacgccaagctacggg</td></tr> | ||
| + | <tr><td>O271</td><td>pUAS-R(BS-6)</td><td>GCAGCACTGCAGCGGCCGCTACTAGTActagagtctccgctcggaggacaga</td></tr> | ||
| + | <tr><td>O272</td><td>P2A-F(S_6-BP)</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGACCAACTTCTCCCTGCTGAAG</td></tr> | ||
| + | <tr><td>O273</td><td>pML2-R(S_BS-6)</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAacgagattccagcaggtcg </td></tr> | ||
| + | <tr><td>O274</td><td>pCMV-F(S_6-BP)</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGcgtgtacggtgggaggtctatataag</td></tr> | ||
| + | <tr><td>O275</td><td>mTagBFP2-F(4-EcoRV-Kozak)</td><td>CGGccccGATATCGCCAccATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGC</td></tr> | ||
| + | <tr><td>O276</td><td>mTagBFP2-F(L1-T2A) </td><td>GGGCAGGGGAAGTCTTCTAACtTGCGGGGACGTGGAGGAAAATCCCGGCCCCGATATCG</td></tr> | ||
| + | <tr><td>O277</td><td>mTagBFP2-F(L2-T2A) </td><td>GCAGCAaCCGGTGGTGAATTCGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC </td></tr> | ||
| + | <tr><td>O278</td><td>mTagBFP2-R(HindIII-6) </td><td>GCAGCAAAGCTTTTAATTAAGtTTGTGCCCCAGTTTGCTAGG </td></tr> | ||
| + | <tr><td>O279</td><td>EBFP2-F(4-EcoRV-Kozak)</td><td>CGGCCCCGATATCGCCAccatggtgagcaagggcg</td></tr> | ||
| + | <tr><td>O280</td><td>EBFP2-R(HindIII-6) </td><td>GCAGCAAAGCTTttacttgtacagctcgtccatg</td></tr> | ||
| + | <tr><td>O281</td><td>mTagBFP2-F(L2-T2A)</td><td>GCAGCAGAATTCGGTaCCGGTGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC</td></tr> | ||
| + | <tr><td>O282</td><td>PIP-F(6-AgeI)</td><td>GCAGCAACCGGTGCCACCATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O283</td><td>ZF43-8-F(6-AgeI)</td><td>GCAGCAACCGGTGCAAGCCCCAGAGTGAGAAC</td></tr> | ||
| + | <tr><td>O284</td><td>PIP-F(6-AgeI-Kozak)</td><td>GCAGCAACCGGTGCCACCatggtgTCAAGGGGGGAGGTTAGAATGG</td></tr> | ||
| + | <tr><td>O285</td><td>ZF43-8-F(6-AgeI-Kozak)</td><td>GCAGCAACCGGTGCCACCatgGCAAGCCCCAGAGTG</td></tr> | ||
| + | <tr><td>O286</td><td>P2A-F(6-SbfI-C)</td><td>GCAGCACCTGCAGGCggctccggcgccac</td></tr> | ||
| + | <tr><td>O287</td><td>KRAB-R(MfeI-6)</td><td>GCAGCACAATTGcaccagccagggttcctc</td></tr> | ||
| + | <tr><td>O288</td><td>mTagBFP2-NR(S)</td><td>GCACTTGAAGTGATGGTTGTCC</td></tr> | ||
| + | <tr><td>O289</td><td>P2A-F(3-AgeI-Kozak-ATG-EcoRI-BamHI-SalI)</td><td>GCAACCGGTGAATTCGCCACCATGGGATCCGTCGACGGATCAGGTGCCACCAAC</td></tr> | ||
| + | <tr><td>O290</td><td>mCherry-R(CAAX-HindIII-1) </td><td>TAAGCTTtcaggagagcacacacttgcagctcatgcagccCTTGTACAGCTCGTCCATG</td></tr> | ||
| + | <tr><td>O291</td><td>mCherry-F(LifeAct-L1) </td><td>AGTCCATCTCCAAGGAGGAGGGCGATCCCCCGGTCGCCACCATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O292</td><td>mCherry-F(LifeAct-L2) </td><td>ATGGGCGTGGCCGACTTGATCAAGAAGTTCGAGTCCATCTCCAAGGAGGAGG</td></tr> | ||
| + | <tr><td>O293</td><td>LifeAct-F(3-EcoRI-BamHI-Kozak)</td><td>GCAGAATTCGGATCCGCCACCATGGGCGTGGCCGAC</td></tr> | ||
| + | <tr><td>O294</td><td>mCherry-R(P2A-L1) </td><td>CGCcggcctgcttcagcagggagaagttggtggcgccggagccCTTGTACAGCTCGTCC</td></tr> | ||
| + | <tr><td>O295</td><td>mCherry-R(P2A-L2) </td><td>TAAGCTTGTCGACCTACAATTGGGGGCCGGGGTTCTCctccacgtcgccggcctgcttc</td></tr> | ||
| + | <tr><td>O296</td><td>Hist2h2bb-F(3-EcoRI-BamHI-Kozak)</td><td>GGTGAATTCGGATCCGCCACCatgccggacccagcgaag</td></tr> | ||
| + | <tr><td>O297</td><td>Hist2h2bb-R(M) </td><td>cgttcacgaaggaGttcatgattcccatagccttagatgagatg</td></tr> | ||
| + | <tr><td>O298</td><td>Hist2h2bb-F(M) </td><td>catgaaCtccttcgtgaacgaCatcttcgagcgcatcgc</td></tr> | ||
| + | <tr><td>O299</td><td>Hist2h2bb-R(OE_mCherry) </td><td>CATTgtGgcCacAggAggGtccttggagctggtgtacttggtg</td></tr> | ||
| + | <tr><td>O300</td><td>mCherry-F(OE_Hist2h2bb) </td><td>acccTccTgtGgcCacAATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O301</td><td>mCherry-R(OE_mito)</td><td>cgcctctcccgttCTTGTACAGCTCGTCCATGCC</td></tr> | ||
| + | <tr><td>O302</td><td>mito-F(OE_mCherry)</td><td>GTACAAGaacgggagaggcggtagac</td></tr> | ||
| + | <tr><td>O303</td><td>mito-R(HindIII-3)</td><td>GCAAAGCTTttaattattttttcttaattgaataattttgataaacgcccc</td></tr> | ||
| + | <tr><td>O304</td><td>Myc-F</td><td>GAGCAGAAATTGATCTCTGAAGAGGAC</td></tr> | ||
| + | <tr><td>O305</td><td>pML2-C0-R</td><td>gcatacattatacgaagttatattaagggttccaag</td></tr> | ||
| + | <tr><td>O306</td><td>mNce-F </td><td>CCCTGCGTCGGTAGCAAC </td></tr> | ||
| + | <tr><td>O307</td><td>tTAA-F(3-MfeI) </td><td>GCACAATTGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG</td></tr> | ||
| + | <tr><td>O308</td><td>tTAA-R(HindIII-3)</td><td>GCAAAGCTTTCATCCTGGGAGCATATCCAGG</td></tr> | ||
| + | <tr><td>O309</td><td>TetOn3G-F(3-MfeI) </td><td>GCACAATTGATGAGCCGACTGGACAAGAG</td></tr> | ||
| + | <tr><td>O310</td><td>TetOn3G-R(HindIII-R)</td><td>GCAAAGCTTTTAGCCCGGCAACATATCAAGATC</td></tr> | ||
| + | <tr><td>O311</td><td>pSV40-F(3-BP) </td><td>GCAGAATTCGCGGCCGCTTCTAGAGgtgtgtcagttagggtgtgg</td></tr> | ||
| + | <tr><td>O312</td><td>pSV40-R(BS-AgeI-3)</td><td>GCAACCGGTctgcagcggccgcTACTAGTAtttgcaaaagcctaggcctcc</td></tr> | ||
| + | <tr><td>O313</td><td>pTight-F(3-BP) </td><td>GCAGAATTCGCGGCCGCTTCTAGAGGCCGGAATTGCTCGAGTTTAC</td></tr> | ||
| + | <tr><td>O314</td><td>pTight-R(BS-AgeI-3)</td><td>GCAACCGGTctgcagcggccgctactagtaGGCGATCTGACGGTTCACTAAAC</td></tr> | ||
| + | <tr><td>O315</td><td>TRE3GV-F(3-BP) </td><td>GCAgaattcgcggccgcttcTAGAGCCCTTTCGTCTTCACTCGAGTTTAC</td></tr> | ||
| + | <tr><td>O316</td><td>TRE3GV-R(BS-AgeI-2)</td><td>CAACCGGTctgcagcggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc</td></tr> | ||
| + | <tr><td>O317</td><td>minCMV-F(3-BP)</td><td>GCAgAATTCGCGGCCGCTTCTAGAGGGTAGGCGTGTACGGTGG</td></tr> | ||
| + | <tr><td>O318</td><td>minCMV2-F(3-BP)</td><td>GCAgaattcgcggccgcttcTAGAGGGTAGGCGTGTACGGTGG</td></tr> | ||
| + | <tr><td>O319</td><td>pSB1C3-VF(S)</td><td>TGCCACCTGACGTCTAAGAA</td></tr> | ||
| + | <tr><td>O320</td><td>pSB1C3-VR(S)</td><td>ATTACCGCCTTTGAGTGAGC</td></tr> | ||
| + | <tr><td>O321</td><td>prefix-mNotch(e)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGCGTCGGTAGCA</td></tr> | ||
| + | <tr><td>O322</td><td>mNotch(e)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACCGTCGGCGCTTTCG</td></tr> | ||
| + | <tr><td>O323</td><td>prefix-hNotch(e)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGTCTTGGAGGGAATCC</td></tr> | ||
| + | <tr><td>O324</td><td>hNotch(e)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACCGGCGTCGTTTTCGAC</td></tr> | ||
| + | <tr><td>O325</td><td>pSB1C3-F</td><td>GCAGCATACTAGTAGCGGCCGCTG</td></tr> | ||
| + | <tr><td>O326</td><td>pSB1C3-R</td><td>GCAGCACTCTAGAAGCGGCCGCG</td></tr> | ||
| + | <tr><td>O327</td><td>prefix-LaG17(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGTGAACCGTCAGATCAGATCCGCTAG</td></tr> | ||
| + | <tr><td>O328</td><td>LaG17(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCA</td></tr> | ||
| + | <tr><td>O329</td><td>prefix-CAR3G(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGCCAGCCAAACCAACAACTACAC</td></tr> | ||
| + | <tr><td>O330</td><td>CAR3G(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccAAGCT</td></tr> | ||
| + | <tr><td>O331</td><td>prefix-EGFPtm(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGTGGAAGAAAATCCCGGTCCG</td></tr> | ||
| + | <tr><td>O332</td><td>EGFPtm(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct</td></tr> | ||
| + | <tr><td>O333</td><td>prefix-tTAA(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGTGTCACGAAAGCGCCG</td></tr> | ||
| + | <tr><td>O334</td><td>tTAA(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct</td></tr> | ||
| + | <tr><td>O335</td><td>IL-2(CO)-F(G_pSB1C3)</td><td>GAAGATCTCACGACGtgaaccgtcagatccgctag</td></tr> | ||
| + | <tr><td>O336</td><td>IL-2(CO)-R(G_pSB1C3)</td><td>GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC</td></tr> | ||
| + | <tr><td>O337</td><td>IL-10(CO)-F(G_pSB1C3)</td><td>GAAGATCTCACGACGtgaaccgtcagatccgctag</td></tr> | ||
| + | <tr><td>O338</td><td>IL-10(CO)-R(G_pSB1C3)</td><td>GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC</td></tr> | ||
| + | <tr><td>O339</td><td>LaG16-2(CO)-F(G_pSB1C3)</td><td>GAAGATCTCACGACGtgaaccgtcagatccgctag</td></tr> | ||
| + | <tr><td>O340</td><td>LaG16-2(CO)-R(G_pSB1C3)</td><td>GATGATCATACGACGGGGTTGCTACCGACGC</td></tr> | ||
| + | <tr><td>O341</td><td>prefix-CD47tm(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr> | ||
| + | <tr><td>O342</td><td>CD47tm(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC</td></tr> | ||
| + | <tr><td>O343</td><td>prefix-hYP9 1scFv(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr> | ||
| + | <tr><td>O344</td><td>hYP9 1scFv(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC</td></tr> | ||
| + | <tr><td>O345</td><td>prefix-LaG 16-2(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr> | ||
| + | <tr><td>O346</td><td>LaG 16-2(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC</td></tr> | ||
| + | <tr><td>O347</td><td>prefix-OKT3scFv(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGGGTGGCGGGGGGTC</td></tr> | ||
| + | <tr><td>O348</td><td>OKT3scFv(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagcTTC</td></tr> | ||
| + | <tr><td>O349</td><td>prefix-Tet-On3G(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGGGAGGTgaattcGCCACC</td></tr> | ||
| + | <tr><td>O350</td><td>Tet-On3G(CO)-R -suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAACCTCCggatccTTAGCC</td></tr> | ||
| + | <tr><td>O351</td><td>prefix-α-CD19(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr> | ||
| + | <tr><td>O352</td><td>α-CD19(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCAG</td></tr> | ||
| + | <tr><td>O353</td><td>prefix-α-CD47(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr> | ||
| + | <tr><td>O354</td><td>α-CD47(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGTAGTTGTTGGTTTGGCTGGC</td></tr> | ||
| + | <tr><td>O355</td><td>prefix-IL-2(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr> | ||
| + | <tr><td>O356</td><td>IL-2(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAATACGAAGTTATATTAAGGGTTCCAAGC</td></tr> | ||
| + | <tr><td>O357</td><td>IL-10(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr> | ||
| + | <tr><td>O358</td><td>IL-10(CO)-R</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagctTC</td></tr> | ||
| + | <tr><td>O359</td><td>pML2-C0(AgeI) </td><td>tgaaccgtcagatccgctagc</td></tr> | ||
| + | <tr><td>O360</td><td>pML2-C0(HindIII)</td><td>atacgaagttatattaagggttccaagc</td></tr> | ||
| + | <tr><td>O361</td><td>mNc-F </td><td>ATACTCGACTACAGTTTTACTGGGG</td></tr> | ||
| + | <tr><td>O362</td><td>LaG2-R(OE_mNc)</td><td>CCCCAGTAAAACTGTAGTCGAGTATAACAGTGACTTGCGTTCCCTG</td></tr> | ||
| + | <tr><td>O363</td><td>LaG17-R(OE_mNc)</td><td>CCCCAGTAAAACTGTAGTCGAGTATGGAAACGGTCACCTGTGTACC</td></tr> | ||
| + | <tr><td>O364</td><td>CD8SP-F(3-AgeI-Kozak) </td><td>GCAACCGGTGCCACCATGGCCCTTCCTGTAACGG</td></tr> | ||
| + | <tr><td>O365</td><td>PDGFRB-R(TGA-HindIII-3)</td><td>GCAAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATGATC</td></tr> | ||
| + | <tr><td>O382</td><td>2*ZF21-16-F(Scar)</td><td>tactagattagaagtgaattagaagtga</td></tr> | ||
| + | <tr><td>O383</td><td>2*ZF21-16-R(Scar)</td><td>ctagtatcacttctaattcacttctaat</td></tr> | ||
| + | <tr><td>O384</td><td>BP-BbsI-F(3) </td><td>ctggaattcgcggccgcttctagaGAAGAC</td></tr> | ||
| + | <tr><td>O385</td><td>3-BP-BbsI-R(Scar)</td><td>ctagtaGTCTTCtctagaagcggccgcgaattccag</td></tr> | ||
| + | <tr><td>O386</td><td>BS-3-F </td><td>TACTAGTAGCGGCCGCTGCAGtcc</td></tr> | ||
| + | <tr><td>O387</td><td>BS-R(3) </td><td>ggaCTGCAGCGGCCGCTA</td></tr> | ||
| + | <tr><td>O388</td><td>EGFP-F(myr-L1)</td><td>ccaaggatgccagccagcggcgccgcGGTGGTGGAGGCTCAATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O389 </td><td>O389 EGFP-F(myr-L2)</td><td>TGAATTCGGATCCGCCACCatgggtagcaacaagagcaagcccaaggatgccagccagc</td></tr> | ||
| + | <tr><td>O390</td><td>2*ZF21-16-A-F(A)</td><td>tactagattagaagtgaattagaagtgatactag</td></tr> | ||
| + | <tr><td>O391</td><td>2*ZF21-16-A-R(A)</td><td>tcacttctaattcacttctaat</td></tr> | ||
| + | <tr><td>O392</td><td>2*ZF21-16-B-F(A)</td><td>attagaagtgaattagaagtga</td></tr> | ||
| + | <tr><td>O393</td><td>2*ZF21-16-B-R(A)</td><td>ctagtatcacttctaattcacttctaatctagta</td></tr> | ||
| + | <tr><td>O394</td><td>BP-F(A) </td><td>GATCCGaattcgcggccgcttctagaGAAGAC</td></tr> | ||
| + | <tr><td>O395</td><td>BP-R(A) </td><td>ctagtaGTCTTCtctagaagcggccgcgaattcg</td></tr> | ||
| + | <tr><td>O396</td><td>BS-F(A) </td><td>TAGCGGCCGCTGCAGA</td></tr> | ||
| + | <tr><td>O397</td><td>BS-R(A) </td><td>AGCTTCTGCAGCGGCCGCTActagta</td></tr> | ||
| + | <tr><td>O398</td><td>BP-F </td><td>Cgaattcgcggccgc</td></tr> | ||
| + | <tr><td>O399</td><td>BS-R </td><td>TCTGCAGCGGCCGC</td></tr> | ||
| + | <tr><td>O400</td><td>pSB1A3-C(3-BP-BamHI) </td><td>GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg</td></tr> | ||
| + | <tr><td>O401</td><td>pSB1A3-C(3-BS-HindIII C)</td><td>GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg</td></tr> | ||
| + | <tr><td>O404</td><td>mNCe-F(M) </td><td>CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG</td></tr> | ||
| + | <tr><td>O405</td><td>mNCe-F </td><td>CCTTGTGTTGGtAGTAATCCATGCTAC</td></tr> | ||
| + | <tr><td>O406</td><td>mNC|e-R(M) </td><td>cctTCTtcttTTTCGTGACAGCAAAACACCGCAGCCTAC</td></tr> | ||
| + | <tr><td>O407</td><td>mNC|e-R </td><td>cctTCTtcttTTTCGTGACAGCAAAAC</td></tr> | ||
| + | <tr><td>O408</td><td>LaG2-R(G_mNCe)</td><td>ggattactaccaacacaaggAACAGTGACTTGCGTTCCCTGC</td></tr> | ||
| + | <tr><td>O409</td><td>LaG17-R(G_mNCe)</td><td>ggattactaccaacacaaggGGAAACGGTCACCTGTGTACC</td></tr> | ||
| + | <tr><td>O410</td><td>tTAA-F(G_mNCe)</td><td>TGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG</td></tr> | ||
| + | <tr><td>O411</td><td>pML2-CX-R(Lin)</td><td>CACCGGTaActcaagATgatctgacTAttcactacagctctgcttatatagacctccc</td></tr> | ||
| + | <tr><td>O412</td><td>pML2-CX-F(Lin)</td><td>TCAATTGGGAACTCGATACCTTCTAATATCTCATAataacttcgtataatgtatgctatacgaagttattaggtccctcgacctgctg</td></tr> | ||
| + | <tr><td>O413</td><td>MCS-F(A) </td><td>TGAATTCGGATCCAGGAGCAGTCTTCGGGCCCGAAGACTGCCATAGTCGACAAGCTTC</td></tr> | ||
| + | <tr><td>O414</td><td>MCS-R(A) </td><td>GAAGCTTGTCGACTATGGCAGTCTTCGGGCCCGAAGACTGCTCCTGGATCCGAATTCA</td></tr> | ||
| + | <tr><td>O415</td><td>pCMV-F2(S) </td><td>cccattgacgcaaatgggc</td></tr> | ||
| + | <tr><td>O416</td><td>CD8aSP-F(Kozak G_pML2-CX)</td><td>gtgaaTAgtcagatcATcttgagTtACCGGTGAATTCGCCACCATGGCGCTGCCGG</td></tr> | ||
| + | <tr><td>O417</td><td>LaG2-R(G_mNCe) </td><td>ATTaTAGCAaGGATTACTACCAACACAAGGAACAGTGACTTGCGTTCCCTGC</td></tr> | ||
| + | <tr><td>O418</td><td>mNCe-F(M) </td><td>CCTTGTGTTGGTAGTAATCCtTGCTAtAATCAAGGGACCTGCGAGC</td></tr> | ||
| + | <tr><td>O419</td><td>mNC-F(M) </td><td>ATACTCGACTAtAGTTTTACaGGGGGGGCGGGCCGGGATATACCCCCG</td></tr> | ||
| + | <tr><td>O420</td><td>mNC|e-R(M) </td><td>CCTTCTTCTTTTTCGTGACAGCAAAACtCCGCAGCCTACGAAGAACAAGAGTAC</td></tr> | ||
| + | <tr><td>O421</td><td>tTAA-F(M) </td><td>GGaGTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGG</td></tr> | ||
| + | <tr><td>O422</td><td>tTAA-R(G_pML2-CX) </td><td>GAGATATTAGAAGGTATCGAGTTCCCAATTGAAGCTTTCATCCTGGGAGCATATCCAGG</td></tr> | ||
| + | <tr><td>O423</td><td>pML2-R(Lin L1)</td><td>tcaagATgatctgacTAttcactacagctctgcttatatagacctcccaccgtacacgc</td></tr> | ||
| + | <tr><td>O424</td><td>pML2-R(Lin L2)</td><td>CGAATTCACCGGTaActcaagATgatctgacTAttcactacagctc</td></tr> | ||
| + | <tr><td>O425</td><td>pML2-F(Lin L2)</td><td>CAAGCTTCAATTGGGAACTCGATACCTTCTAATATCTC</td></tr> | ||
| + | <tr><td>O426</td><td>pML2-CX(EcoRI) </td><td>gtagtgaaTAgtcagatcATcttgagTtACCGGTG</td></tr> | ||
| + | <tr><td>O427</td><td>pML2-CX(HindIII)</td><td>TATGAGATATTAGAAGGTATCGAGTTCCCAATTGAAGC</td></tr> | ||
| + | <tr><td>O428</td><td>pML2X-R(Lin1)</td><td>AAGACCACCGGTGAATTCattaagggttccggttccactaggtacccacc</td></tr> | ||
| + | <tr><td>O429</td><td>pML2X-R(Lin2)</td><td>CGTCGACTAGCCCTAACGTGATCTGACAGTACATTAAGACCACCGGTGAATTCattaag</td></tr> | ||
| + | <tr><td>O430</td><td>pML2X-F(Lin1)</td><td>TGAtaggtccctcgacctgctggaatctcgtg</td></tr> | ||
| + | <tr><td>O431</td><td>pML2X-F(Lin2)</td><td>GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAtaggtccctcgacctgc</td></tr> | ||
| + | <tr><td>O432</td><td>MCS-F(A) </td><td>AGTCGACGGATCCAGGAGCAGTCTTCCTGCAGGAAGACTGCCATACAATTGAAGCTTC</td></tr> | ||
| + | <tr><td>O433</td><td>MCS-R(A) </td><td>GAAGCTTCAATTGTATGGCAGTCTTCCTGCAGGAAGACTGCTCCTGGATCCGTCGACT</td></tr> | ||
| + | <tr><td>O434</td><td>pML2X-F(S) </td><td>atcctccctttatccagccc</td></tr> | ||
| + | <tr><td>O435</td><td>pML2X-R(S) </td><td>atcgataagctcgcttcacg</td></tr> | ||
| + | <tr><td>O436</td><td>pML2Y-F(Lin1) </td><td>CGGCCGCtaggtccctcgacctgctggaatctcgtg</td></tr> | ||
| + | <tr><td>O437</td><td>pML2Y-F(Lin2) </td><td>GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAGCGGCCGCtaggtccctc</td></tr> | ||
| + | <tr><td>O438</td><td>pML2Y(G_SalI) </td><td>GGTCTTAATGTACTGTCAGATCACGTTAGGGC</td></tr> | ||
| + | <tr><td>O439</td><td>pML2Y(G_HindIII)</td><td>TCACACTTCGACTAGACTGGTACTTGAATGAGG</td></tr> | ||
| + | <tr><td>O440</td><td>pML2X-R(Lin2)</td><td>CGTCGACTAGCCCTAACGTGATCTGACAGTACAaTAAGACCACCGGTGAATTCattaag</td></tr> | ||
| + | <tr><td>O441</td><td>pML2Y(G_SalI)</td><td>GGTCTTAtTGTACTGTCAGATCACGTTAGGGC</td></tr> | ||
| + | <tr><td>O442</td><td>CMV-F(3-EcoRI)</td><td>GCAGAATTCcgttacataacttacggtaaatggcccgcctggctg</td></tr> | ||
| + | <tr><td>O443</td><td>pCMV-R(AgeI-3)</td><td>GCAACCGGTagctctgcttatatagacctcccaccgtacacgcc</td></tr> | ||
| + | <tr><td>O444</td><td>PGK-F(3-EcoRI) </td><td>GCAGAATTCgggtaggggaggc</td></tr> | ||
| + | <tr><td>O445</td><td>PGK-R(AgeI-G_pML2Y-SalI)</td><td>CGTCGACTAGCCCTAACGTGATCTGACAGTACAATAAGACCACCGGTcgaaaggcccgg</td></tr> | ||
| + | <tr><td>O446</td><td>CD8aSP-F(G_pML2Y) </td><td>GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCG</td></tr> | ||
| + | <tr><td>O447</td><td>P2A-R(G_CD8aSP) </td><td>tacaggaagggccatCGGACCGGGATTTTCTTCCACGTC</td></tr> | ||
| + | <tr><td>O448</td><td>CD8aSP-F(G_P2A) </td><td>gaaaatcccggtccgATGGCCCTTCCTGTAACG</td></tr> | ||
| + | <tr><td>O449</td><td>PDGFRBtr-R(G_pML2Y)</td><td>TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGC</td></tr> | ||
| + | <tr><td>O450</td><td>CD8aSP-F(G_pML2Y)</td><td>GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCG</td></tr> | ||
| + | <tr><td>O451</td><td>tTAA-R(G_pML2Y) </td><td>TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATC</td></tr> | ||
| + | <tr><td>O452</td><td>LaG17-R(G_mNCe)</td><td>attatagcaaggattactaccaacacaaggGGAAACGGTCACCTGTGTACCTTGTCC</td></tr> | ||
| + | <tr><td>O453</td><td>aCD19-R(G_mNCe)</td><td>attatagcaaggattactaccaacacaaggTGATGAAACGGTTACGGATGTCCCTTGTC</td></tr> | ||
| + | <tr><td>O454</td><td>LaG16-2-R(G_mNC)</td><td>CGCCCCCCCTGTAAAACTATAGTCGAGTATAACAGTGACTTGCGTTCCCTGCC</td></tr> | ||
| + | <tr><td>O455</td><td>LaG17-R(G_mNC)</td><td>cgccccccctgtaaaactatagtcgagtatGGAAACGGTCACCTGTGTACCTTGTCC</td></tr> | ||
| + | <tr><td>O456</td><td>aCD19-R(G_mNC)</td><td>CGCCCCCCCTGTAAAACTATAGTCGAGTATTGATGAAACGGTTACGGATGTCCCTTGTC</td></tr> | ||
| + | <tr><td>O457</td><td>CD19ECD-R(G_Myc)</td><td>gatcaatttctgctcCTTCCATCCCCCCGTGCG</td></tr> | ||
| + | <tr><td>O458</td><td>Myc-F(G_CD19ECD)</td><td>acggggggatggaagGAGCAGAAATTGATCTCTGAAGAG</td></tr> | ||
| + | <tr><td>O459</td><td>PGK-F(S)</td><td>cggcattctgcacgcttc</td></tr> | ||
| + | <tr><td>O460</td><td>LaG16-F(L1)</td><td>acgccgccaggccgGAGCAGAAGCTGATCTCAGAGGAGGACCTGATGGCCCAGGTGCAG</td></tr> | ||
| + | <tr><td>O461</td><td>LaG16-F(L2)</td><td>ggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggc</td></tr> | ||
| + | <tr><td>O462</td><td>LaG16-F(L3)</td><td>tgaaccgtcagatccgctagcgctaCCGGTatggccttaccagtgaccg </td></tr> | ||
| + | <tr><td>O463</td><td>mNC|e-R(M)</td><td>CCTTCTTCTTTTTCGTGACAGCAAAACTCCACAGCCCACAAAGAACAGG </td></tr> | ||
| + | <tr><td>O464</td><td>pML2Y-R(C) </td><td>ACCACCGGTGAATTCattaagggttccggttccactaggtacccaccaaattccggcgc</td></tr> | ||
| + | <tr><td>O465</td><td>pML2Y-F(C) </td><td>TGAGCGGCCGCtaggtccctcgacctgctggaatctcgtgaagcgagcttatcg</td></tr> | ||
| + | <tr><td>O466</td><td>XXX-F(1-AgeI-pML2Y-SalI) </td><td>CACCGGTGGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACccggggtggtgc</td></tr> | ||
| + | <tr><td>O467</td><td>XXX-R(HindIII-pML2Y-NotI-1)</td><td>aGCGGCCGCTCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTcccggcggcg</td></tr> | ||
| + | <tr><td>O468</td><td>ZF 21-16</td><td>GCAGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaTTAGAAGTGaTACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O469</td><td>EGFP-F(3-SalI-Kozak-ATG-BamHI) </td><td>CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGGAG</td></tr> | ||
| + | <tr><td>O470</td><td>EGFP/mCherry-R(L1) </td><td>GCTTGCTTTAACAGAGAGAAGTTCGTGGCgccggagccCTTGTACAGCTCGTCCATGCC</td></tr> | ||
| + | <tr><td>O471</td><td>EGFP-R(L2) </td><td>TCAATTGAGGTCCCGGGTTTTCTTCCACGTCTCCTGCTTGCTTTAACAGAGAGAAGTTC</td></tr> | ||
| + | <tr><td>O472</td><td>P2A-R(MfeI-BbsI-TGA-SbfI-BbsI-HindIII-3)</td><td>GGAAGCTTAGTCTTCCTGCAGGTCATGGAAGACTCAATTGAGGTCCCGGGTTTTCTTCC</td></tr> | ||
| + | <tr><td>O473</td><td>CD8aSP-F(3-SalI-Kozak)</td><td>GCAGTCGACGCCACCatggccttaccagtgaccg</td></tr> | ||
| + | <tr><td>O474</td><td>TRE3GV-F(3-EcoRI)</td><td>GCAGAATTCGAGTTTACTCCCTATCagtgatagagaacgtatgaag</td></tr> | ||
| + | <tr><td>O475</td><td>TRE3GV-R(AgeI-3)</td><td>GCAACCGGTAAGTATAAGACAAAAGTGTTGTGGAATTGcTCC</td></tr> | ||
| + | <tr><td>O476</td><td>mCherry-R(KOD)</td><td>CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCAC</td></tr> | ||
| + | <tr><td>O477</td><td>mCherry-F(KOD)</td><td>CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC</td></tr> | ||
| + | <tr><td>O478</td><td>BP-F(M_3-EcoRI) </td><td>GCAGAATTCcggccgcttcTAGAG</td></tr> | ||
| + | <tr><td>O479</td><td>BS-R(M_1-PstI-AgeI-3)</td><td>GCAACCGGTCTGCAGacggccgcTACTAGT</td></tr> | ||
| + | <tr><td>O480</td><td>ZF 43-8</td><td>GCAGAATTCGCGGCCGCTTCTAGaGAGTGAGGAcaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAG</td></tr> | ||
| + | <tr><td>O481</td><td>HIST2H2BE-F(CE_pML2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGcctgaaccggcaaaatcc</td></tr> | ||
| + | <tr><td>O482</td><td>HIST2H2BE-R(CE_EGFP)</td><td>tccttggagctggtgtacttggtgac</td></tr> | ||
| + | <tr><td>O483</td><td>EGFP-F(CE_HIST2H2BE)</td><td>aagtacaccagctccaaggaCccTccTgtGgcCacAATGGTGAGCAAGGGCGAG</td></tr> | ||
| + | <tr><td>O484</td><td>P2A-R(CE_pML2Y) </td><td>GTACTTGAATGAGGGAAGCTTAGTCTTCCTGCAGGTC</td></tr> | ||
| + | <tr><td>O485</td><td>LifeAct-F(3-SalI)</td><td>CTAGTCGACGCCACCATGGGCGTGGCCGAC</td></tr> | ||
| + | <tr><td>O486</td><td>myr-F(3-SalI)</td><td>CTAGTCGACGCCACCATGggtagcaacaagagcaagcccaaggatgccagccagc</td></tr> | ||
| + | <tr><td>O487</td><td>1-BP-21-16-43-8-SpePI-1-F(A)</td><td>CGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTG</td></tr> | ||
| + | <tr><td>O488</td><td>1-BP-21-16-43-8-SpeI-1-R(A)</td><td>CACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAAGCGGCCGCGAATTCG</td></tr> | ||
| + | <tr><td>O489</td><td>1-XbaI-21-16-43-8-SpeI-BS-1-F(A)</td><td>GTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAGC</td></tr> | ||
| + | <tr><td>O490</td><td>1-XbaI-21-16-43-8-SpeI-BS-1-R(A)</td><td>GCTGCAGCGGCCGCTACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAC</td></tr> | ||
| + | <tr><td>O491</td><td>p2Y(CE_EcoRI) </td><td>accggaacccttaatGAATTC</td></tr> | ||
| + | <tr><td>O492</td><td>p2Y(CE_AgeI) </td><td>CAGTACAATAAGACCACCGGT</td></tr> | ||
| + | <tr><td>O493</td><td>p2Y(CE_SalI) </td><td>ATCACGTTAGGGCTAGTCGAC</td></tr> | ||
| + | <tr><td>O494</td><td>p2Y(CE_HindIII)</td><td>GTACTTGAATGAGGGAAGCTT</td></tr> | ||
| + | <tr><td>O495</td><td>mNC-F(M) </td><td>ATTCTCGACTACAGTTTCACAGGAGGAGCGGGCCGGGATATACCCCCG</td></tr> | ||
| + | <tr><td>O496</td><td>tTAA-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATCCAGGTC</td></tr> | ||
| + | <tr><td>O497</td><td>CD8aSP-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGG</td></tr> | ||
| + | <tr><td>O498</td><td>LaG2-R(CE_mNC)</td><td>GTGAAACTGTAGTCGAGAATAACAGTGACTTGCGTTCCC</td></tr> | ||
| + | <tr><td>O499</td><td>LaG17-R(CE_mNC)</td><td>GTGAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACC</td></tr> | ||
| + | <tr><td>O500</td><td>aCD19-R(CD_mNC) </td><td>GTGAAACTGTAGTCGAGAATTGATGAAACGGTTACGGATGTCC</td></tr> | ||
| + | <tr><td>O501</td><td>PGK-F(CE_p2Y) </td><td>accggaacccttaatGAATTCgggtaggggaggcgc</td></tr> | ||
| + | <tr><td>O502</td><td>PGK-R(CE_p2Y) </td><td>CAGTACAATAAGACCACCGGTcgaaaggcccggagatgag</td></tr> | ||
| + | <tr><td>O503</td><td>CD8aSP-F2(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCGGTC</td></tr> | ||
| + | <tr><td>O504</td><td>CD19ECD(CE_Myc) </td><td>atttctgctcCTTCCATCCCCCCGTGC</td></tr> | ||
| + | <tr><td>O505</td><td>Myf-F(CE_CD19ECD)</td><td>gggatggaagGAGCAGAAATTGATCTCTGAAGAGGAC</td></tr> | ||
| + | <tr><td>O506</td><td>PDGFRBtr-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATG</td></tr> | ||
| + | <tr><td>O507</td><td>TetOn3G-F(CE_P2A)</td><td>TGGAAGAAAACCCGGGACCTATGAGCCGACTGGACAAGAGC</td></tr> | ||
| + | <tr><td>O508</td><td>TetOn3G-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTTACAATTGGCCCGGCAACATATCAAGATCAAAGTC</td></tr> | ||
| + | <tr><td>O509</td><td>U105 pEGFP-C5</td><td>CATGGTCCTGCTGGAGTTCGT</td></tr> | ||
| + | <tr><td>O510</td><td>mCherry-R(KOD)</td><td>CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCTTGCAGGGAGGAGTCCTGGGTCAC</td></tr> | ||
| + | <tr><td>O511</td><td>mCherry-F(KOD)</td><td>CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACCATGGGCTGGGAGGCC</td></tr> | ||
| + | <tr><td>O512</td><td>BP-F(M CE_p2Y-EcoRI)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAG</td></tr> | ||
| + | <tr><td>O513</td><td>BS(M CE_p2Y-AgeI) </td><td>CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT</td></tr> | ||
| + | <tr><td>O514</td><td>BS-R(M CE_p2Y-AS) </td><td>CCCTAACGTGATCTGACAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT</td></tr> | ||
| + | <tr><td>O515</td><td>PIP-F(CE_p2Y-AS-Kozak)</td><td>ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O516</td><td>KRAB-R(CE_HindIII) </td><td>GTACTTGAATGAGGGAAGCTTTTACACCAGCCAGGGTTCC</td></tr> | ||
| + | <tr><td>O517</td><td>pSV40-F(CE_p2Y-EcoRI)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAGgtgtgtcagttagggtgtgg</td></tr> | ||
| + | <tr><td>O518</td><td>ZF21-16-F(CE_p2Y-EcoRI) </td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaTTAGAAGTG</td></tr> | ||
| + | <tr><td>O519</td><td>CE_P2A-R </td><td>AGGTCCCGGGTTTTCTTCC</td></tr> | ||
| + | <tr><td>O520</td><td>TetOn3G-F(CE_P2A-MfeI) </td><td>TGGAAGAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGC</td></tr> | ||
| + | <tr><td>O521</td><td>TetOn3G-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTC</td></tr> | ||
| + | <tr><td>O522</td><td>TRE3GV-F(1-BP-M_p2Y) </td><td>CGAATTCcggccgcttcTAGAGGAGTTTACTCCCTATCagtgatagagaacgtatgaag</td></tr> | ||
| + | <tr><td>O523</td><td>TRE3GV-R(BS-M_p2Y-AgeI-1)</td><td>GACCGGTCTGCAGacggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc</td></tr> | ||
| + | <tr><td>O524</td><td>ZF43-8-F(CE_p2Y M) </td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaGAGTGAGG</td></tr> | ||
| + | <tr><td>O525</td><td>LaG16-F(CE-P2Y_SalI)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGGTCaccgccttgctcctgc</td></tr> | ||
| + | <tr><td>O526</td><td>ZF43-8-F(CE_P2A) </td><td>TGGAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC</td></tr> | ||
| + | <tr><td>O527</td><td>ZF43-8-R </td><td>GGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtctgttc</td></tr> | ||
| + | <tr><td>O528</td><td>G4S-SV40NLS-F(CE_SynTF-C)</td><td>ATACAGGTGAGAAGGGCTCCGGAGGAGGAGGTAGTCCAAAAAAG</td></tr> | ||
| + | <tr><td>O529</td><td>KRAB-R(CE_p2Y-HindIII) </td><td>GTACTTGAATGAGGGAAGCTTTCACAATTGCACCAGCCAAGGTTCTTCTCccttttcc</td></tr> | ||
| + | <tr><td>O530</td><td>PIP-F(CE_P2A) </td><td>TGGAAGAAAACCCGGGACCTATGTCAAGGGGGGAGGTTAG</td></tr> | ||
| + | <tr><td>O531</td><td>ZF43-8-F(CE_p2Y-SalI)</td><td>ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGAG</td></tr> | ||
| + | <tr><td>O532</td><td>HA-F(CE_pGEX)</td><td>TTCCAGGGGCCCCTGGGATCCTACCCCTATGATGTACCAGATTACGC</td></tr> | ||
| + | <tr><td>O533</td><td>Myc-R(CE_pGEX)</td><td>CTCGAGTCGACCCGGGAATTCTCACAGGTCCTCTTCAGAGATCAATTTC</td></tr> | ||
| + | <tr><td>O534</td><td>TetOn3G-F(CE_P2A-MfeI) </td><td>GAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGCAAGGTAATC </td></tr> | ||
| + | <tr><td>O535</td><td>TetOn3G-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTCGTCG</td></tr> | ||
| + | <tr><td>O536</td><td>CD8aSP-F(CE_P2A) </td><td>GTCCGATGGCCCTTCCTGTAACGG </td></tr> | ||
| + | <tr><td>O537</td><td>PDGFRBtr(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGCCAGAG</td></tr> | ||
| + | <tr><td>O538</td><td>T2A-F(CE_p2Y-SalI-BamHI) </td><td>ATCACGTTAGGGCTAGTCGACGCAGGATCCggAtcAggTGAAGGTAGGGGGTCATTGC</td></tr> | ||
| + | <tr><td>O539</td><td>hYP9.1bscFv-R(CE_OKT3scFV)</td><td>GACTGTTGGAGTTGAACCTGACTACCTCCTCCACCCTTGATCTCAAGTTTGGTTCCTTG</td></tr> | ||
| + | <tr><td>O540</td><td>OKT3scFv-F </td><td>CAGGTTCAACTCCAACAGTCTGG </td></tr> | ||
| + | <tr><td>O541</td><td>OKT3scFv-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTCTAGCGGTTTATTTCAAGTTTTGTGCC </td></tr> | ||
| + | <tr><td>O542</td><td>2.3D11scFv-R(CE_CAR3G)</td><td>GTAGTTGTTGGTTTGGCTGGCTTTATTTCGACTTTCGTGCCTCC</td></tr> | ||
| + | <tr><td>O543</td><td>CAR3G-F </td><td>CCAGCCAAACCAACAACTACAC </td></tr> | ||
| + | <tr><td>O544</td><td>CAR3G-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTCACCGTGGTGGCAAAGCC </td></tr> | ||
| + | <tr><td>O545</td><td>IL-10-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTCAGTTCCTTATTTTCATCGTCATATACGCTTC</td></tr> | ||
| + | <tr><td>O546</td><td>LaG17-R(CE_hNC)</td><td>GAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACCTTGTCCC</td></tr> | ||
| + | <tr><td>O547</td><td>hNC-F(M) </td><td>ATTCTCGACTACAGTTTCGGTGGGGGAGCGGG </td></tr> | ||
| + | <tr><td>O548</td><td>hNC|e-R(M) </td><td>CCTTCTTCTTTTTCGTGACAGCAAAACTCCACACCCTACG </td></tr> | ||
| + | <tr><td>O549</td><td>mTagBFP2-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGTGTCTAAGGGCGAAGAG</td></tr> | ||
| + | <tr><td>O550</td><td>mTagBFP2-R(M1) </td><td>CGTCCTCGTATGTGGTGACTCTCTCCCATGTGAAGCCC </td></tr> | ||
| + | <tr><td>O551</td><td>mTagBFP2-F(M2) </td><td>AGTCACCACATACGAGGACGGGGGCGTGCTGACCG </td></tr> | ||
| + | <tr><td>O552</td><td>mTagBFP2-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCAATTCAGCTTGTGCCCCAGTTTG </td></tr> | ||
| + | <tr><td>O553</td><td>mNc F(CE/G_LNR-A [-])</td><td>ATTCTCGACTACAGTTTCACAGGAGGAGCGTCACTTAACTTCAATGATCCGTGGAAG</td></tr> | ||
| + | <tr><td>O554</td><td>mNc F(CE/G_LNR-ABLnkr[-])</td><td>ATTCTCGACTACAGTTTCACAGGAGGAGCGTGCACCCAGTCCCTCCA</td></tr> | ||
| + | <tr><td>O555</td><td>mNc F(CE_LNR-A[-]hlx3+)</td><td>ATTCTCGACTACAGTTTCACAATCACGCCTGCGGTT</td></tr> | ||
| + | <tr><td>O556</td><td>mNC-R(M_S2)</td><td>GTTCGCTTTTgtcctCTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC</td></tr> | ||
| + | <tr><td>O557</td><td>mNC-F(M_S2)</td><td>CGAAGaggacAAAAGCGAACCTGTCGAACCGCC </td></tr> | ||
| + | <tr><td>O558</td><td>mNC-R(M_S3)</td><td>GCAAAAagaagagcagTACGAAGAACAAGAGTACGAACGCAGCAGC </td></tr> | ||
| + | <tr><td>O559</td><td>mNC-F(M_S3)</td><td>CGTActgctcttctTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGG </td></tr> | ||
| + | <tr><td>O560</td><td>MycoP-F</td><td>CACCATCTGTCACTCTGTTAA</td></tr> | ||
| + | <tr><td>O561</td><td>MycoP-R</td><td>GGAGCAAACAGGATTAGATAC</td></tr> | ||
| + | <tr><td>O562</td><td>BP-ZF21-16-43-8-BS</td><td>TTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTG</td></tr> | ||
| + | <tr><td>O563</td><td>CE ZF21-16-43-8 F</td><td>TAAGGATGATTTCTGGAATTCGCGGCCGCTTc</td></tr> | ||
| + | <tr><td>O564</td><td>CE ZF21-16-43-8 R</td><td>ttgcccttttttgccggaCTGCAGCGGCCGctac</td></tr> | ||
| + | <tr><td>O565</td><td>mNC-R-N(S)</td><td>CCTCTGTCAGTTGGCAGTC</td></tr> | ||
| + | <tr><td>O566</td><td>pGEX usual F(S)</td><td>GAGCTGCATGTGTCAGAGG</td></tr> | ||
| + | <tr><td>O567</td><td>pGEX usual R(S)</td><td>GCAAGCCACGTTTGGTG</td></tr> | ||
| + | <tr><td>O568</td><td>d2EGFP-R(L1) </td><td>CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTCTTGTACAGCTCGTCCATG</td></tr> | ||
| + | <tr><td>O569</td><td>d2EGFP-R(L2) </td><td>ATCCCGCTCTCCTGGGCACAAGACATGGGCAGCGTGCCATCATCCTGCTCCTCCACCTC</td></tr> | ||
| + | <tr><td>O570</td><td>d2EGFP-R(L3) </td><td>CACATTGATCCTAGCAGAAGCACAGGCTGCTGGGTGACGGTCCATCCCGCTCTCCTGG</td></tr> | ||
| + | <tr><td>O571</td><td>d2EGFP-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTCTACACATTGATCCTAGCAGAAGC </td></tr> | ||
| + | <tr><td>O572</td><td>CD8aSP-F(CE_p2Y G3)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG</td></tr> | ||
| + | <tr><td>O573</td><td>EGFP-R(CE_mN1ce) </td><td>GGATTACTACCAACACAAGGCTTGTACAGCTCGTCCATGCC </td></tr> | ||
| + | <tr><td>O574</td><td>mN1c-R(C) </td><td>CCTTCTTCTTTTTCGTGACAGCAAAACTCCGC </td></tr> | ||
| + | <tr><td>O575</td><td>p2Y-F(C_HindIII) </td><td>AAGCTTCCCTCATTCAAGTACCAGTCTAGTCG</td></tr> | ||
| + | <tr><td>O576</td><td>VP64-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTCAGagcatGtcCagGtcGaagtcatctagggcatcgg</td></tr> | ||
| + | <tr><td>O577</td><td>Gal4-F(CE_m|hN1c) </td><td>TCACGAAAAAGAAGAAGGATGAAGCTGCTGAGCAGCatc </td></tr> | ||
| + | <tr><td>O578</td><td>ZF21-16-F(CE_m|hN1c)</td><td>TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC </td></tr> | ||
| + | <tr><td>O579</td><td>ZF42-10-F(CE_mN1c)</td><td>TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCAC </td></tr> | ||
| + | <tr><td>O580</td><td>ZF43-8-F(CE_mN1c) </td><td>TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC</td></tr> | ||
| + | <tr><td>O581</td><td>KRAB-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTCACACCAGCCAAGGTTCTTCGCccttttccagcc</td></tr> | ||
| + | <tr><td>O582</td><td>rtTAA-F(CE_m|hN1c|e) </td><td>TCACGAAAAAGAAGAAGGatgtctagactggacaagagc </td></tr> | ||
| + | <tr><td>O583</td><td>rtTAA-R(CE_p2Y-HindIII)</td><td>GTACTTGAATGAGGGAAGCTTTCAcccggggagcatgtcaag</td></tr> | ||
| + | <tr><td>O584</td><td>mN1c-F-C(S) </td><td>CCAGTTGCACCTCATGTACG </td></tr> | ||
| + | <tr><td>O585</td><td>mTagBFP2-R(L1)</td><td>CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTATTCAGCTTGTGCCCCAG</td></tr> | ||
| + | <tr><td>O586</td><td>rtTAA-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCatgtctagactggacaagagcaaag</td></tr> | ||
| + | <tr><td>O587</td><td>rtTAA-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTttacccggggagcatgtcaag </td></tr> | ||
| + | <tr><td>O588</td><td>VP64-R(MfeI CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCACAATTGtagcatatcCagatcaaagtcatctaggg</td></tr> | ||
| + | <tr><td>O589</td><td>ZF21-16-F(CE_P2A) </td><td>GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC</td></tr> | ||
| + | <tr><td>O590</td><td>ZF42-10-F(M CE_p2Y)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaGACGCTG</td></tr> | ||
| + | <tr><td>O591</td><td>CMV-F(BP_M)</td><td>tGAATTCcggccgcttcTAGAGcgttacataacttacggtaaatg </td></tr> | ||
| + | <tr><td>O592</td><td>CMV-R(BS_M)</td><td>cACCGGTCTGCAGacggccgcTACTAGTAagctctgcttatatagacctc</td></tr> | ||
| + | <tr><td>O593</td><td>TetO-R(BS_M)</td><td>cACCGGTCTGCAGacggccgcTACTAGTATCTCTATCACTGATAGGGAgtaaactggat</td></tr> | ||
| + | <tr><td>O594</td><td>EGFP-F(L1_palm)</td><td>CaaaatgcggctgctgcttcgcccgggaCccaccCgtcgccaccATGGTGAGCAAGGGC</td></tr> | ||
| + | <tr><td>O595</td><td>EGFP-F(L2_palm)</td><td>Gggggtcgctgtgcctgtccccgtgtgatccgaaaagtgctggcaaaatgcggctgctg</td></tr> | ||
| + | <tr><td>O596</td><td>palm-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCatgcggggggggcacaaagggggtcgctgtgc</td></tr> | ||
| + | <tr><td>O597</td><td>mCherry-F(CE_mN1c-SV40NLS)</td><td>TCACGAAAAAGAAGAAGGCCAAAAAAGAAGAGAAAGGTAATGGTGAGCAAGGGCGAGG</td></tr> | ||
| + | <tr><td>O598</td><td>mCherry-R(L_HA) </td><td>AGCGTAATCTGGTACGTCGTATGGGTACTTGTACAGCTCGTCCATGC </td></tr> | ||
| + | <tr><td>O599</td><td>HA-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCAAGCGTAATCTGGTACGTCGTATG </td></tr> | ||
| + | <tr><td>O600</td><td>mCherry(CE_mN1c)</td><td>TCACGAAAAAGAAGAAGGATGGTGAGCAAGGGCGAGG </td></tr> | ||
| + | <tr><td>O601</td><td>mN1c-R(M_S1)</td><td>TCGAGTTCTGCAGCTGCAGCGCCACCACTCGTGCCCGG </td></tr> | ||
| + | <tr><td>O602</td><td>mN1c-F(M_S1)</td><td>GCTGCAGCTGCAGAACTCGATCCTATGGACATACGGGGGTCC</td></tr> | ||
| + | <tr><td>O603</td><td>mN1c-R(M_S2)</td><td>GTTCGCTTTTGTGCACTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC</td></tr> | ||
| + | <tr><td>O604</td><td>mN1c-F(M_S2)</td><td>CGAAGTGCACAAAAGCGAACCTGTCGAACCGCC </td></tr> | ||
| + | <tr><td>O605</td><td>CD8aSP-F(CE_p2Y) </td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG</td></tr> | ||
| + | <tr><td>O606</td><td>PDGFRB-R </td><td>GCGAGGTTTCTTTTGCCAG </td></tr> | ||
| + | <tr><td>O607</td><td>mCherry-F(CE_PDGFRB)</td><td>TGGCAAAAGAAACCTCGCATGGTGAGCAAGGGCGAGG </td></tr> | ||
| + | <tr><td>O608</td><td>mCherry-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCACTTGTACAGCTCGTCCATGC </td></tr> | ||
| + | <tr><td>O609</td><td>tTAA-F(CE_PDGFRB)</td><td>TCTGGCAAAAGAAACCTCGCATGAGTCGATTGGATAAATCTAAGGTAATTAACAG</td></tr> | ||
| + | <tr><td>O610</td><td>minCMV-R(L1_ZF21-16)</td><td>cgcTACTAGTAtCACTTCTAAttCACTTCTAAtCTCTAgTAggcgatctgacggttcac</td></tr> | ||
| + | <tr><td>O611</td><td>minCMV-R(L2_ZF21-16)</td><td>CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAtCACTTCTAAttCAC</td></tr> | ||
| + | <tr><td>O612</td><td>minCMV-R(L1_ZF43-8)</td><td>cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAGGCGATCTGACGGTTCAC</td></tr> | ||
| + | <tr><td>O613</td><td>minCMV-R(L2_ZF43-8)</td><td>CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAgTCCTCACTCtg </td></tr> | ||
| + | <tr><td>O614</td><td>tTAA-R-N(S)</td><td>CCCCTCGATACCTACTTCGTTC</td></tr> | ||
| + | <tr><td>O615</td><td>UAS-R(CE_minCMV）</td><td>CTCTAGTActagagtctccgctcggaggacaga </td></tr> | ||
| + | <tr><td>O616</td><td>minCMV-F(CE_UAS)</td><td>cggagactctagTACTAGAGGGTAGGCGTGTACGGTGG</td></tr> | ||
| + | <tr><td>O617</td><td>pSV40-R(L_ZF43-8)</td><td>cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAtttgcaaaagcctaggcc</td></tr> | ||
| + | <tr><td>O618</td><td>mNc F (OE-test)</td><td>ATTCTCGACTACAGTTTCACAGGAG</td></tr> | ||
| + | <tr><td>O619</td><td>mNc R (OE-test)</td><td>CCTTCTTCTTTTTCGTGACAGCAAAA</td></tr> | ||
| + | <tr><td>O620</td><td>LNRA-R</td><td>GCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O621</td><td>LNRB-F</td><td>TGCACCCAGTCCCTCCAAT</td></tr> | ||
| + | <tr><td>O622</td><td>LNRA R (OE_AB lnkr-WT10)</td><td>GTTCTTCCACGGATCATTGAAGTTAAG</td></tr> | ||
| + | <tr><td>O623</td><td>LNRB F (OE_AB lnkr-WT10)</td><td>TCACTTAACTTCAATGATCCGTGGAAG</td></tr> | ||
| + | <tr><td>O624</td><td>LNR A R (OE_ABlnkr-WT 8)</td><td>CTTCCACGGATCATTGAAGTTAAGTG</td></tr> | ||
| + | <tr><td>O625</td><td>LNR B F (OE_AB lnkr-WT8)</td><td>CTTAACTTCAATGATCCGTGGAAGAAC</td></tr> | ||
| + | <tr><td>O626</td><td>LNR A R (OE_AB lnkr-WT6)</td><td>CCACGGATCATTGAAGTTAAGTGAG</td></tr> | ||
| + | <tr><td>O627</td><td>LNR B F (OE_AB lnkr-WT6)</td><td>AACTTCAATGATCCGTGGAAGAAC</td></tr> | ||
| + | <tr><td>O628</td><td>LNR A R (OE_AB lnkr-WT4)</td><td>CGGATCATTGAAGTTAAGTGAGCAG</td></tr> | ||
| + | <tr><td>O629</td><td>LNR B F (OE_AB lnkr-WT4)</td><td>TTCAATGATCCGTGGAAGAACTGC</td></tr> | ||
| + | <tr><td>O630</td><td>LNR A R (OE_AB lnkr-WT2)</td><td>ATCATTGAAGTTAAGTGAGCAGTCACC</td></tr> | ||
| + | <tr><td>O631</td><td>LNR B F (OE_AB lnkr-WT2)</td><td>AATGATCCGTGGAAGAACTGCAC</td></tr> | ||
| + | <tr><td>O632</td><td>LNR A-lnkr R(OE mut L-I )</td><td>AGTTCTTCCACGGATCATTGAAGTTGATTGAGCAGTCACCCCCGT</td></tr> | ||
| + | <tr><td>O633</td><td>LNR B-lnkr F(OE mut L-I )</td><td>ACGGGGGTGACTGCTCAATCAACTTCAATGATCCGTGGAAGAACT</td></tr> | ||
| + | <tr><td>O634</td><td>LNR A-lnkr R(OE mut L-V)</td><td>AGTTCTTCCACGGATCATTGAAGTTCACTGAGCAGTCACCCCCGT</td></tr> | ||
| + | <tr><td>O635</td><td>LNR B-lnkr F(OE mut L-V )</td><td>ACGGGGGTGACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACT</td></tr> | ||
| + | <tr><td>O636</td><td>LNR A-lnkr R(OE mut L-A)</td><td>AGTTCTTCCACGGATCATTGAAGTTGGCTGAGCAGTCACCCCCGT</td></tr> | ||
| + | <tr><td>O637</td><td>LNR B-lnkr F(OE mut L-A )</td><td>ACGGGGGTGACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACT</td></tr> | ||
| + | <tr><td>O638</td><td>LNR A-lnkr R(OE mut L-G)</td><td>AGTTCTTCCACGGATCATTGAAGTTGCCTGAGCAGTCACCCCCGT</td></tr> | ||
| + | <tr><td>O639</td><td>LNR B-lnkr F(OE mut L-G )</td><td>ACGGGGGTGACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACT</td></tr> | ||
| + | <tr><td>O640</td><td>LNR A-lnkr R(OE mut S-G)</td><td>GTTCTTCCACGGATCATTGAAGTTAAGGCCGCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O641</td><td>LNR B-lnkr F(OE mut S-G )</td><td>GGACGGGGGTGACTGCGGCCTTAACTTCAATGATCCGTGGAAGAAC</td></tr> | ||
| + | <tr><td>O642</td><td>LNR A-lnkr R(OE mut N-Q)</td><td>GCAGTTCTTCCACGGATCATTGAATTGAAGTGAGCAGTCACCCCC</td></tr> | ||
| + | <tr><td>O643</td><td>LNR B-lnkr F(OE mut N-Q )</td><td>GGGGGTGACTGCTCACTTCAATTCAATGATCCGTGGAAGAACTGC</td></tr> | ||
| + | <tr><td>O644</td><td>LNR A-lnkr R(OE mut N-V)</td><td>GCAGTTCTTCCACGGATCATTGAAAACAAGTGAGCAGTCACCCCC</td></tr> | ||
| + | <tr><td>O645</td><td>LNR B-lnkr F(OE mut N-V )</td><td>GGGGGTGACTGCTCACTTGTTTTCAATGATCCGTGGAAGAACTGC</td></tr> | ||
| + | <tr><td>O646</td><td>LNR A-lnkr R(OE mut S-A)</td><td>GTTCTTCCACGGATCATTGAAGTTAAGGGCGCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O647</td><td>LNR B-lnkr F(OE mut S-A )</td><td>GGACGGGGGTGACTGCGCCCTTAACTTCAATGATCCGTGGAAGAAC</td></tr> | ||
| + | <tr><td>O648</td><td>IL10-F(L1)</td><td>TGCTTACATGCGGAGATGTGGAGGAGAATCCGGGACCAATGCATTCTAGCGCTCTTTTG</td></tr> | ||
| + | <tr><td>O649</td><td>IL10-F(L2)</td><td>GAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTG</td></tr> | ||
| + | <tr><td>O650</td><td>TetOn3G-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGAGCCGACTGGACAAG</td></tr> | ||
| + | <tr><td>O651</td><td>mNc F(CE_LNR-A[-]hlx3+)√</td><td>ATTCTCGACTACAGTTTCACAAATCACGCCTGCGGTT</td></tr> | ||
| + | <tr><td>O652</td><td>LaG17 R(CE_G6-LNR-A[-]hlx3+)</td><td>ACCGCCTCCACCGCCTCCGGAAACGGTCACCTGTGTACC</td></tr> | ||
| + | <tr><td>O653</td><td>mNc F(CE_G6-LNR-A[-]hlx3+)</td><td>GGAGGCGGTGGAGGCGGTAATCACGCCTGCGGTTG</td></tr> | ||
| + | <tr><td>O654</td><td>mNc F(CE_G6-LNR-A[-])</td><td>GGAGGCGGTGGAGGCGGTTCACTTAACTTCAATGATCCGTGGAAG</td></tr> | ||
| + | <tr><td>O655</td><td>LNR A-lnkr R(OE mut L-I )</td><td>TTGAAGTTAAGTGAGCAGTCACCCCCGTCCCAAC</td></tr> | ||
| + | <tr><td>O656</td><td>LNR B-lnkr F(OE mut L-I )</td><td>GACTGCTCACTTAACTTCAATGATCCGTGGAAGAACTGCACCC</td></tr> | ||
| + | <tr><td>O657</td><td>LNR A-lnkr R(OE mut L-V)</td><td>TTGAAGTTCACTGAGCAGTCACCCCCGTCCCAAC</td></tr> | ||
| + | <tr><td>O658</td><td>LNR B-lnkr F(OE mut L-V )</td><td>GACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACTGCACCC</td></tr> | ||
| + | <tr><td>O659</td><td>LNR A-lnkr R(OE mut L-A)</td><td>TTGAAGTTGGCTGAGCAGTCACCCCCGTCCCAAC</td></tr> | ||
| + | <tr><td>O660</td><td>LNR B-lnkr F(OE mut L-A )</td><td>GACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACTGCACCC</td></tr> | ||
| + | <tr><td>O661</td><td>LNR A-lnkr R(OE mut L-G)</td><td>TTGAAGTTGCCTGAGCAGTCACCCCCGTCCCAAC</td></tr> | ||
| + | <tr><td>O662</td><td>LNR B-lnkr F(OE mut L-G )</td><td>GACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACTGCACCC</td></tr> | ||
| + | <tr><td>O663</td><td>LNR A-lnkr R(OE mut S-G)</td><td>TTGAAGTTAAGGCCGCAGTCACCCCCGTCCCAACC</td></tr> | ||
| + | <tr><td>O664</td><td>LNR B-lnkr F(OE mut S-G )</td><td>GACTGCGGCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC</td></tr> | ||
| + | <tr><td>O665</td><td>LNR A-lnkr R(OE mut N-Q)</td><td>TTGAACTGAAGTGAGCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O666</td><td>LNR B-lnkr F(OE mut N-Q )</td><td>GACTGCTCACTTCAGTTCAATGATCCGTGGAAGAACTGCACCCAG</td></tr> | ||
| + | <tr><td>O667</td><td>LNR A-lnkr R(OE mut N-V)</td><td>TTGAACACAAGTGAGCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O668</td><td>LNR B-lnkr F(OE mut N-V )</td><td>GACTGCTCACTTGTGTTCAATGATCCGTGGAAGAACTGCACCCAG</td></tr> | ||
| + | <tr><td>O669</td><td>LNR A-lnkr R(OE mut S-A)</td><td>TTGAAGTTAAGGGCGCAGTCACCCCCGTCCCAACC</td></tr> | ||
| + | <tr><td>O670</td><td>LNR B-lnkr F(OE mut S-A )</td><td>GACTGCGCCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC</td></tr> | ||
| + | <tr><td>O671</td><td>LNR A-lnkr R(OE mut N-L)</td><td>TTGAACAGAAGTGAGCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O672</td><td>LNR B-lnkr F(OE mut N-L )</td><td>GACTGCTCACTTCTGTTCAATGATCCGTGGAAGAACTGCACCCAG</td></tr> | ||
| + | <tr><td>O673</td><td>LNR A-lnkr R(OE mut N-G)</td><td>TTGAAGCCAAGTGAGCAGTCACCCCCGTCC</td></tr> | ||
| + | <tr><td>O674</td><td>LNR B-lnkr F(OE mut N-G )</td><td>GACTGCTCACTTGGCTTCAATGATCCGTGGAAGAACTGCACCCAG</td></tr> | ||
| + | <tr><td>O675</td><td>tTAA-F(CE_p2A)</td><td>GAAGAAAACCCGGGACCTATGAGTCGATTGGATAAATCTAAGGTAATTAACAG</td></tr> | ||
| + | <tr><td>O676</td><td>rtTAA-F(CE_p2A)</td><td>GAAGAAAACCCGGGACCTatgtctagactggacaagagcaaag</td></tr> | ||
| + | <tr><td>O677</td><td>CD19ECD-F(3-BamHI) </td><td>CTGGGATCCGAAGAGCCTCTCGTCGTGAAAG</td></tr> | ||
| + | <tr><td>O678</td><td>CD19ECD-R(TAA-EcoRI-3)</td><td>CGGGAATTCTTACTTCCATCCCCCCGTGC</td></tr> | ||
| + | <tr><td>O679</td><td>Gal4-R(CE_VP64)</td><td>agaAcctccActTccAccagcagctgcCACTGTCAGCTGCCGCTGgc</td></tr> | ||
| + | <tr><td>O680</td><td>VP64-F(CE_Gal4)</td><td>ggTggAagTggaggTtctggCgggAGcgatgctttagacgattttgacttagatatgc</td></tr> | ||
| + | <tr><td>O681</td><td>VP64-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCAtgatccGagcatGtcCagGtcGaagtcatctaggg</td></tr> | ||
| + | <tr><td>O682</td><td>Gal4-F(CE_P2A)</td><td>GAAGAAAACCCGGGACCTATGAAGCTGCTGAGCAGCatc</td></tr> | ||
| + | <tr><td>O683</td><td>mCherry-F-C(S)</td><td>ACCTCCCACAACGAGGAC</td></tr> | ||
| + | <tr><td>O684</td><td>SynTF-F-N(CE_P2A)</td><td>GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O685</td><td>mScarlet-F(3-SalI-Kozak-ATG-BamHI)</td><td>CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGG</td></tr> | ||
| + | <tr><td>O686</td><td>mScarlet-R(M) </td><td>CTTGCGGTCcACGTTGTAGGCGCCG</td></tr> | ||
| + | <tr><td>O687</td><td>mScarlet-F(M) </td><td>TACAACGTgGACCGCAAGTTGGACATCAC</td></tr> | ||
| + | <tr><td>O688</td><td>G4S-R(C) </td><td>ACATCGGCCATTGAACCACCCCCACCGGAACC</td></tr> | ||
| + | <tr><td>O689</td><td>LaG17-F(CE_G4S)</td><td>TGGTTCAATGGCCGATGTGCAGC</td></tr> | ||
| + | <tr><td>O690</td><td>mN1c-F-N(C) </td><td>ATTCTCGACTACAGTTTCACAGGAGGAGCG</td></tr> | ||
| + | <tr><td>O691</td><td>HA-R(C) </td><td>CGCGTAATCTGGTACATCATAGGGGTAGGG</td></tr> | ||
| + | <tr><td>O692</td><td>LaG17-F(CE_HA)</td><td>GATGTACCAGATTACGCGATGGCCGATGTGCAGC</td></tr> | ||
| + | <tr><td>O693</td><td>LaG17-R </td><td>GGAAACGGTCACCTGTGTACC</td></tr> | ||
| + | <tr><td>O694</td><td>G4S-F(CE_LaG17)</td><td>ACACAGGTGACCGTTTCCGGGGGGGGTGGCAGTGG</td></tr> | ||
| + | <tr><td>O695</td><td>SynZF-F(CE_p2Y-Kozak)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O696</td><td>TRE3GV-F(1-BP_M_p2Y)</td><td>CGAATTCcggccgcttcTAGAGTCCCTATCagtgatagagaacgtatgaag</td></tr> | ||
| + | <tr><td>O697</td><td>4xUAS-F(1-BP_M_p2Y)</td><td>tGAATTCcggccgcttcTAGAGcgccaagctacggg</td></tr> | ||
| + | <tr><td>O698</td><td>4xUAS-R(1-BS_M_p2Y)</td><td>cACCGGTCTGCAGacggccgcTACTAGTAcgctcggaggacaga</td></tr> | ||
| + | <tr><td>O699</td><td>CMV-R(L1_21-43)</td><td>TCTAAtCTAGTAgTCCTCACTCttCACTTCTAAtCTAGTAagctctgcttatatagacc</td></tr> | ||
| + | <tr><td>O724</td><td>pol-F-C </td><td>GCCCTCAAAGTAGACGGC</td></tr> | ||
| + | <tr><td>O725</td><td>ori-F-3</td><td>CGCCTGGTATCTTTATAGTCCTG</td></tr> | ||
| + | <tr><td>O726</td><td>tTAA-R(C) </td><td>TCCTGGGAGCATATCCAGGTCAAGATCGTC </td></tr> | ||
| + | <tr><td>O727</td><td>CD8ASP-F(L1_P2A) </td><td>CTCCCTGCTGAAGCAAGCAGGAGACGTGGAAGAAAACCCGGGACCTATGGCGCTGCCGG</td></tr> | ||
| + | <tr><td>O728</td><td>CD8ASP-F(L2_P2A CE_tTAA)</td><td>CTGGATATGCTCCCAGGAggctccggcGCCACCAACTTCTCCCTGCTGAAGCAAGC </td></tr> | ||
| + | <tr><td>O729</td><td>LaG2-F-N(S) </td><td>GTGCTGCCTCCGGTC </td></tr> | ||
| + | <tr><td>O730</td><td>tTAA-F-C(S) </td><td>GCTTAAGTGCGAGAGTGGC </td></tr> | ||
| + | <tr><td>O731</td><td>ACD8b-F-N(S) </td><td>TATCCGGGGTCTCCCTCC </td></tr> | ||
| + | <tr><td>O732</td><td>Gal4DBD-R-N(S) </td><td>ttggcgcacttgggc </td></tr> | ||
| + | <tr><td>O733</td><td>TetOn3G-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGGAGGTgaattcGCCACC</td></tr> | ||
| + | <tr><td>O734</td><td>TetOn3G-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTACCTCCggatccTTAGCC</td></tr> | ||
| + | <tr><td>O735</td><td>T2A-F(CE_p2Y) </td><td>ATCACGTTAGGGCTAGTCGACggAtcAggTGAGGGTCGG</td></tr> | ||
| + | <tr><td>O736</td><td>2.3D11scFv-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCACTTTATTTCGACTTTCGTGCCTCC</td></tr> | ||
| + | <tr><td>O737</td><td>CAR3G-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACCCAGCCAAACCAACAACTACAC</td></tr> | ||
| + | |||
| + | <tr><td>O747</td><td>CD8A-F(CE_P2A) </td><td>GAAGAAAACCCGGGACCTATGGCGCTGCCGG</td></tr> | ||
| + | <tr><td>O748</td><td>SynPro_space_5-F(CE_p2Y)</td><td>accggaacccttaatGAATTCCGGGTTTCGTAACAATCGCATGAGGATTCGCAACGC</td></tr> | ||
| + | <tr><td>O749</td><td>SynPro_space_7/7-F(CE_p2Y)</td><td>accggaacccttaatGAATTCGCCTAGATCATCGTTGGC</td></tr> | ||
| + | <tr><td>O750</td><td>SynPro_space_6/7-F(CE_p2Y)</td><td>accggaacccttaatGAATTCCGCACGTATCACTTGATC</td></tr> | ||
| + | <tr><td>O751</td><td>SynPro_space_5/7-F(CE_p2Y)</td><td>accggaacccttaatGAATTCCTTTCCACGATCATGTGC</td></tr> | ||
| + | <tr><td>O752</td><td>SynPro_space_4/4-F(CE_p2Y)</td><td>accggaacccttaatGAATTCGACTGTTGCGAACGATTC</td></tr> | ||
| + | <tr><td>O753</td><td>SynPro_space_3/4-F(CE_p2Y)</td><td>accggaacccttaatGAATTCTCCCGTCTCAGTAAAGG</td></tr> | ||
| + | <tr><td>O754</td><td>SynPro_space_2/4-F(CE_p2Y)</td><td>accggaacccttaatGAATTCAATCGGACTGCCTTCG</td></tr> | ||
| + | <tr><td>O755</td><td>SynPro_space_1/4-F(CE_p2Y)</td><td>accggaacccttaatGAATTCGTATCAGTCGCCTCGG</td></tr> | ||
| + | <tr><td>O756</td><td>minCMV2-F(L_space3) </td><td>CGTAAGAGGCTCACTCTCCCTTACACGGAGTGGATAGGTAGGCGTGTACGG</td></tr> | ||
| + | <tr><td>O757</td><td>minCMV2-R(CE_p2Y) </td><td>CAGTACAaTAAGACCACCGGTAAGTATAAGACAAAAGTGTTGTGG</td></tr> | ||
| + | <tr><td>O758</td><td>ZF21.16_8/8-F(L) </td><td>GAGGATTCGCAACGCaTTAGAAGTGaGCCTAGATCATCGTTGGC</td></tr> | ||
| + | <tr><td>O759</td><td>ZF21.16_7/8-F(L) </td><td>GCCTAGATCATCGTTGGCaTTAGAAGTGaCGCACGTATCACTTGATC</td></tr> | ||
| + | <tr><td>O760</td><td>ZF21.16_6/8-F(L) </td><td>CGCACGTATCACTTGATCaTTAGAAGTGaCTTTCCACGATCATGTGC</td></tr> | ||
| + | <tr><td>O761</td><td>ZF21.16_5/8-F(L) </td><td>CTTTCCACGATCATGTGCaTTAGAAGTGaGACTGTTGCGAACGATTC</td></tr> | ||
| + | <tr><td>O762</td><td>ZF21.16_4/8-F(L) </td><td>GACTGTTGCGAACGATTCaTTAGAAGTGaTCCCGTCTCAGTAAAGG</td></tr> | ||
| + | <tr><td>O763</td><td>ZF21.16_3/8-F(L) </td><td>TCCCGTCTCAGTAAAGGTaTTAGAAGTGaAATCGGACTGCCTTCG</td></tr> | ||
| + | <tr><td>O764</td><td>ZF21.16_2/8-F(L) </td><td>AATCGGACTGCCTTCGTAaTTAGAAGTGaGTATCAGTCGCCTCGG</td></tr> | ||
| + | <tr><td>O765</td><td>ZF21.16_1/8-F(L) </td><td>GTATCAGTCGCCTCGGAAaTTAGAAGTGaCGTAAGAGGCTCACTCTC</td></tr> | ||
| + | <tr><td>O766</td><td>TetO_6/6-F(L)</td><td>CGCACGTATCACTTGATCTCCCTATCagtgatagagaCTTTCCACGATCATGTGC</td></tr> | ||
| + | <tr><td>O767</td><td>TetO_5/6-F(L)</td><td>CTTTCCACGATCATGTGCTCCCTATCagtgatagagaGACTGTTGCGAACGATTC</td></tr> | ||
| + | <tr><td>O768</td><td>TetO_4/6-F(L)</td><td>GACTGTTGCGAACGATTCTCCCTATCagtgatagagaTCCCGTCTCAGTAAAGG</td></tr> | ||
| + | <tr><td>O769</td><td>TetO_3/6-F(L)</td><td>TCCCGTCTCAGTAAAGGTTCCCTATCagtgatagagaAATCGGACTGCCTTCG</td></tr> | ||
| + | <tr><td>O770</td><td>TetO_2/6-F(L)</td><td>AATCGGACTGCCTTCGTATCCCTATCagtgatagagaGTATCAGTCGCCTCGG</td></tr> | ||
| + | <tr><td>O771</td><td>TetO_1/6-F(L)</td><td>GTATCAGTCGCCTCGGAATCCCTATCagtgatagagaCGTAAGAGGCTCACTCTC</td></tr> | ||
| + | <tr><td>O772</td><td>minCMV2-F(CE_p2Y)</td><td>accggaacccttaatGAATTCGGTAGGCGTGTACGGTGG</td></tr> | ||
| + | <tr><td>O773</td><td>SV40NLS-KRAB-F(CE_P2A)</td><td>GAAGAAAACCCGGGACCTATGGGCCCAAAAAAGAAGAGAAAGGTAatggacg</td></tr> | ||
| + | <tr><td>O774</td><td>KRAB-G4S-R </td><td>ACTACCTCCTCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag</td></tr> | ||
| + | <tr><td>O775</td><td>TetR-F(CE_KRAB-G4S) </td><td>TTGTAGGTGGAGGAGGTAGTATGAGTCGATTGGATAAATCTAAGG</td></tr> | ||
| + | <tr><td>O776</td><td>TetR-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCAAGAGCCACTCTCGCACTTAAG</td></tr> | ||
| + | <tr><td>O777</td><td>Gal4-F(CE_KRAB-G4S)</td><td>TTGTAGGTGGAGGAGGTAGTATGAAGCTGCTGAGCAGCatc</td></tr> | ||
| + | <tr><td>O778</td><td>Gal4-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCACACTGTCAGCTGCCGC</td></tr> | ||
| + | <tr><td>O779</td><td>SynZFN-F(CE_KRAB-G4S)</td><td>TTGTAGGTGGAGGAGGTAGTATGGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O780</td><td>ZF21.16-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGTGTTTTCAGATGc</td></tr> | ||
| + | <tr><td>O781</td><td>ZF42.10-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGGGTTTTCAGATGc</td></tr> | ||
| + | <tr><td>O782</td><td>ZF43.8-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtc</td></tr> | ||
| + | <tr><td>O783</td><td>7xTetO-R(CE_CMVe)</td><td>accgtaagttatgtaacgGCCTACCTCGACATACG</td></tr> | ||
| + | <tr><td>O784</td><td>4xUAS-F(CE_p2Y)</td><td>accggaacccttaatGAATTCcgccaagctacggg</td></tr> | ||
| + | <tr><td>O785</td><td>4xUAS-R(CE_CMVe)</td><td>accgtaagttatgtaacgGTActagagtctccgc</td></tr> | ||
| + | <tr><td>O786</td><td>8xZFO-R(CE_CMVe)</td><td>accgtaagttatgtaacgCACGCCTACCCTCTAG</td></tr> | ||
| + | <tr><td>O787</td><td>VP64-F(CE_p2Y) </td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGgatgctttagacgattttgacttag</td></tr> | ||
| + | <tr><td>O788</td><td>VP64-G4S-R </td><td>ACTACCTCCTCCACCAagcatGtcCagGtcGaagtcatc</td></tr> | ||
| + | <tr><td>O789</td><td>SynZF-F(CE_VP64_G4S)</td><td>tgctTGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O790</td><td>CfaN-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACCTG</td></tr> | ||
| + | <tr><td>O791</td><td>KRAB-F(CE_p2Y) </td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGgacgccaagagcctg</td></tr> | ||
| + | <tr><td>O792</td><td>SynZF-F(CE_KRAB_G4S)</td><td>TTGTAGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O793</td><td>CfaC-F(CE_p2Y) </td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGG</td></tr> | ||
| + | <tr><td>O794</td><td>SynZF-R(SV40NLS-CE_p2Y)</td><td>TACTTGAATGAGGGAAGCTTTCATACCTTTCTCTTCTTTTTTGGGGAGCCCTTCTCACC</td></tr> | ||
| + | <tr><td>O795</td><td>KRAB-R(CE_NES) </td><td>GTCAATCTTTCCAGGGGCGGCAGTACAAGCCACGGTTCCTCGCccttttccagcctaag</td></tr> | ||
| + | <tr><td>O796</td><td>SynZF-F(L1_dNLS)</td><td>GATCAGAGAACTTGTATTTTCAAAGTGGCAAGAAGAAGAAAATGGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O797</td><td>SynZF-F(L2_dNLS)</td><td>GCCCCTGGAAAGATTGACGCTGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC</td></tr> | ||
| + | <tr><td>O798</td><td>KRAB-R </td><td>TACAAGCCACGGTTCCTCGCccttttccagcctaag</td></tr> | ||
| + | <tr><td>O799</td><td>dNLS-F(CE_KRAB)</td><td>GAGGAACCGTGGCTTGTAAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC</td></tr> | ||
| + | <tr><td>O800</td><td>KRAB-R(CE_TCS)</td><td>CAAGTTCTCTGATCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag</td></tr> | ||
| + | <tr><td>O801</td><td>SynZF-F(CE_TCS)</td><td>GGTGGATCAGAGAACTTGTATTTTCAAAGTGGCGGAATGGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O802</td><td>TEVp-F(L1_NLS)</td><td>GGTGCCAAAAAAGAAGAGAAAGGTAGGGGAGAGCCTGTTCAAAG</td></tr> | ||
| + | <tr><td>O803</td><td>TEVp-F(L2_NLS)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGTGCCAAAAAAGAAGAGAAAGG</td></tr> | ||
| + | <tr><td>O804</td><td>TEVpN-R(CE_CfaN)</td><td>CGTGTCGTATGAGAGGCATGTCTGAAAATTAGTGGTTACCAAAC</td></tr> | ||
| + | <tr><td>O805</td><td>CfaN-F </td><td>TGCCTCTCATACGACACGGAAATTTTGACCGTC</td></tr> | ||
| + | <tr><td>O806</td><td>CfaN-R(CE_p2Y) </td><td>GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACC</td></tr> | ||
| + | <tr><td>O807</td><td>CfaC-F </td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGGAAGAGC</td></tr> | ||
| + | <tr><td>O808</td><td>CfaC-R </td><td>GTTTGAAGCGACGAGACCATTTTTAAGGAGG</td></tr> | ||
| + | <tr><td>O809</td><td>TEVpC-F(CE_CfaC)</td><td>GGTCTCGTCGCTTCAAACTGCGCCGAAAAGAGCATGTCCTCCATGG</td></tr> | ||
| + | <tr><td>O810</td><td>BS-R(CE_minCMV2)</td><td>ACGCCTACCTCGACATACGTCACGCCTACCCTCTAG</td></tr> | ||
| + | <tr><td>O811</td><td>minCMV2-F </td><td>ACGTATGTCGAGGTAGGCGT</td></tr> | ||
| + | <tr><td>O812</td><td>VP64-R </td><td>CagcatGtcCagGtcGaagtcatc</td></tr> | ||
| + | <tr><td>O813</td><td>dNLS-F(CE_VP64)</td><td>ttCgaCctGgaCatgctGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC</td></tr> | ||
| + | <tr><td>O814</td><td>SynZFC-R(CE_T2AN)</td><td>CCTACCTTCAccTgaTccGGAGCCCTTCTCACCTGT</td></tr> | ||
| + | <tr><td>O815</td><td>SV40NLS-F(L1_T2A) </td><td>GCTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAGTGCCAAAAAAGAAGAGAAAGG</td></tr> | ||
| + | <tr><td>O816</td><td>T2A-F </td><td>ggAtcAggTGAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTGG</td></tr> | ||
| + | <tr><td>O817</td><td>TEVpC-R(NLS-CE_T2A)</td><td>CCTACCTTCAccTgaTccTACCTTTCTCTTCTTTTTTGGCTGGCTATAGACCAATTCG</td></tr> | ||
| + | <tr><td>O818</td><td>VP64-F(L_T2A) </td><td>TACATGCGGAGATGTGGAGGAGAATCCTGGACCAgatgctttagacgattttgacttag</td></tr> | ||
| + | <tr><td>O819</td><td>KRAB-F(L_T2A)</td><td>CTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAATGgacgccaagagcc</td></tr> | ||
| + | |||
| + | <tr><td>O824</td><td>CE_p2Y-SalI(CE_pSB1C3-XbaI) </td><td>gaattcgcggccgcttctagagATCACGTTAGGGCTAGTCGAC</td></tr> | ||
| + | <tr><td>O825</td><td>CE_p2Y-HindIII(CE_pSB1C3-SpeI)</td><td>actgcagcggccgctactagtaGTACTTGAATGAGGGAAGCTT</td></tr> | ||
| + | <tr><td>O826</td><td>CE_p2Y-EcoRI(CE_pSB1C3-XbaI)</td><td>gaattcgcggccgcttctagagaccggaacccttaatGAATTC</td></tr> | ||
| + | <tr><td>O827</td><td>CE_p2Y-AgeI(CE_pSB1C3-SpeI)</td><td>actgcagcggccgctactagtaCAGTACAaTAAGACCACCGGT</td></tr> | ||
| + | <tr><td>O828</td><td>mN1c-F(CE_BP)</td><td>gaattcgcggccgcttctagagATTCTCGACTACAGTTTC</td></tr> | ||
| + | <tr><td>O829</td><td>mN1c-R(CE_BS)</td><td>actgcagcggccgctactagtaCCTTCTTCTTTTTCGTGA</td></tr> | ||
| + | <tr><td>O830</td><td>mN1ce-F(CE_BP)</td><td>gaattcgcggccgcttctagagCCTTGTGTTGGTAGTAATCC</td></tr> | ||
| + | <tr><td>O831</td><td>LaG2-F(CE_BP)</td><td>gaattcgcggccgcttctagagATGGCTCAGGTCCAGCTTG</td></tr> | ||
| + | <tr><td>O832</td><td>LaG2-R(CE_BS)</td><td>actgcagcggccgctactagtaAACAGTGACTTGCGTTCCC</td></tr> | ||
| + | <tr><td>O833</td><td>LaG16(CE_BP) </td><td>gaattcgcggccgcttctagagATGGCACAGGTCCAACTCG</td></tr> | ||
| + | <tr><td>O834</td><td>LaG16-R(CE_BS)</td><td>actgcagcggccgctactagtaGCTTACCGTTACTTGTGTGCC</td></tr> | ||
| + | <tr><td>O835</td><td>LaG17-F(CE_BP)</td><td>gaattcgcggccgcttctagagATGGCCGATGTGCAGC</td></tr> | ||
| + | <tr><td>O836</td><td>LaG17-R(CE_BS)</td><td>actgcagcggccgctactagtaGGAAACGGTCACCTGTGTACC</td></tr> | ||
| + | <tr><td>O837</td><td>ACD19-F(CE_BP)</td><td>gaattcgcggccgcttctagagGATATACAAATGACCCAGACTACGTCC</td></tr> | ||
| + | <tr><td>O838</td><td>ACD19-R(CE_BS)</td><td>actgcagcggccgctactagtaTGATGAAACGGTTACGGATGTCC</td></tr> | ||
| + | <tr><td>O839</td><td>CfaN-F(CE_BP)</td><td>gaattcgcggccgcttctagagTGCCTCTCATATGACACGG</td></tr> | ||
| + | <tr><td>O840</td><td>CfaN-R(CE_BS)</td><td>actgcagcggccgctactagtaTGGCAACCCATCGACCTG</td></tr> | ||
| + | <tr><td>O841</td><td>CfaC-F(CE_BP)</td><td>gaattcgcggccgcttctagagATGGTTAAGATCATATCACGGAAGAGC</td></tr> | ||
| + | <tr><td>O842</td><td>CfaC-R(CE_BS)</td><td>actgcagcggccgctactagtaGTTTGAAGCTACGAGACCATTTTTAAGG</td></tr> | ||
| + | <tr><td>O843</td><td>TEVN(CE_BP)</td><td>gaattcgcggccgcttctagagGGGGAGAGCCTGTTCAAAGG</td></tr> | ||
| + | <tr><td>O844</td><td>TEVpN(CE_BS)</td><td>actgcagcggccgctactagtaTGTCTGAAAATTAGTGGTTACCAAACAAATG</td></tr> | ||
| + | <tr><td>O845</td><td>TEVpC-F(CE_BP)</td><td>gaattcgcggccgcttctagagTGCGCCGAAAAGAGCATGTCCTCCATGGTTAG</td></tr> | ||
| + | <tr><td>O846</td><td>TEVpC-R(CE_BS)</td><td>actgcagcggccgctactagtaCTGGCTATAGACCAATTCGTTCATC</td></tr> | ||
| + | <tr><td>O847</td><td>SynZFN-F(CE_BP) </td><td>gaattcgcggccgcttctagagatgGCAAGTCCTAGGGTG</td></tr> | ||
| + | <tr><td>O848</td><td>ZF21.16N-R(CE_BS)</td><td>actgcagcggccgctactagtaCTGGAAGGGCTTTTCTCCG</td></tr> | ||
| + | <tr><td>O849</td><td>ZF21.16C-F(CE_BP)</td><td>gaattcgcggccgcttctagagTGTAGGATATGCATGCGAAACTTCTC</td></tr> | ||
| + | <tr><td>O850</td><td>SynZFC-R(CE_BS) </td><td>actgcagcggccgctactagtaGGAGCCCTTCTCACCTGT</td></tr> | ||
| + | </table></div> | ||
| + | </div> | ||
| + | <div class="section container" id="gblock"> | ||
| + | <h2>gBlocks</h2> | ||
| + | <pre>> alphaCD19(CO) | ||
| + | tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG | ||
| + | CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCTACCCCTATGATGTACCAGAT | ||
| + | TACGCGGATATACAAATGACCCAGACTACGTCCAGCCTTTCCGCCTCCCTGGGTGATCGC | ||
| + | GTGACAATCAGTTGTAGGGCTAGTCAGGACATAAGTAAATACCTCAACTGGTATCAACAG | ||
| + | AAGCCTGACGGAACAGTTAAACTTCTTATCTATCATACAAGTAGGCTTCATTCAGGCGTT | ||
| + | CCATCCAGATTTAGTGGATCTGGGAGCGGCACCGACTACTCACTCACGATTAGTAATCTG | ||
| + | GAACAAGAGGACATCGCCACTTATTTTTGCCAGCAGGGTAACACTCTTCCCTATACATTC | ||
| + | GGCGGCGGTACGAAACTCGAAATTACAGGGGGGGGTGGCAGTGGTGGCGGAGGTTCCGGT | ||
| + | GGGGGTGGTTCAGAAGTAAAACTTCAGGAATCTGGTCCAGGGCTGGTTGCACCCAGCCAA | ||
| + | TCCCTTTCCGTCACGTGCACCGTATCCGGGGTCTCCCTCCCAGACTACGGCGTGAGTTGG | ||
| + | ATACGACAGCCGCCGCGCAAGGGGCTTGAGTGGCTGGGTGTCATTTGGGGATCTGAAACA | ||
| + | ACGTATTATAATTCCGCTTTGAAATCCCGCCTCACTATTATCAAGGACAATTCTAAGAGC | ||
| + | CAGGTCTTTCTGAAGATGAACAGCCTGCAAACGGATGACACAGCCATCTACTATTGTGCA | ||
| + | AAGCACTATTACTATGGTGGTTCATACGCTATGGATTACTGGGGACAAGGGACATCCGTA | ||
| + | ACCGTTTCATCACCCTGCGTCGGTAGCAACCC</pre> | ||
| + | <pre>> tTAA(CO) | ||
| + | TGTCACGAAAGCGCCGACGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAGCGCAC | ||
| + | TGGAGCTTCTGAACGAAGTAGGTATCGAGGGGCTCACCACACGAAAATTGGCACAGAAGC | ||
| + | TCGGAGTGGAGCAGCCGACTTTGTACTGGCACGTTAAGAATAAGAGGGCTTTGCTTGACG | ||
| + | CGCTGGCCATTGAGATGCTCGACCGGCATCACACTCACTTCTGCCCGCTTGAAGGCGAGA | ||
| + | GTTGGCAAGATTTCCTTAGGAACAATGCAAAGAGCTTTCGATGCGCCCTGCTTAGCCATC | ||
| + | GGGACGGCGCAAAGGTTCATCTTGGGACTCGGCCTACTGAGAAGCAATATGAAACCCTGG | ||
| + | AAAACCAGCTTGCTTTCCTTTGCCAACAGGGGTTCTCACTGGAGAATGCCCTGTACGCAC | ||
| + | TCTCCGCGGTCGGCCATTTTACTCTCGGGTGTGTTTTGGAAGATCAGGAACACCAGGTAG | ||
| + | CGAAAGAGGAGCGAGAAACGCCTACAACTGATTCCATGCCACCGTTGCTGAGACAAGCTA | ||
| + | TAGAGTTGTTCGACCACCAGGGTGCTGAACCGGCGTTTCTCTTTGGTCTGGAACTCATTA | ||
| + | TCTGCGGCCTTGAAAAGCAGCTTAAGTGCGAGAGTGGCGGTCCCGCAGATGCACTTGATG | ||
| + | ACTTTGATTTGGATATGTTGCCCGCGGACGCTCTTGATGACTTCGATCTGGATATGCTGC | ||
| + | CGGCTGATGCCCTCGACGATCTTGACCTGGATATGCTCCCAGGATGAaagcttggaaccc | ||
| + | ttaatataacttcgtat</pre> | ||
| + | <pre>> TetOn3G(CO) | ||
| + | GGAGGTgaattcGCCACCATGAGCCGACTGGACAAGAGCAAGGTAATCAATTCAGCTTTG | ||
| + | GAGCTTCTGAACGGCGTGGGAATCGAGGGTCTCACCACCAGAAAGCTCGCGCAAAAATTG | ||
| + | GGGGTGGAACAACCGACTCTCTATTGGCATGTTAAGAACAAGAGGGCCCTCCTTGACGCA | ||
| + | CTCCCCATCGAAATGCTGGATCGACATCACACACATAGTTGTCCGCTGGAGGGGGAGTCT | ||
| + | TGGCAAGATTTCCTCAGGAATAACGCGAAGAGTTATCGGTGCGCTCTCCTTAGTCATCGC | ||
| + | GATGGAGCGAAAGTGCATCTGGGGACAAGACCGACGGAAAAACAATATGAGACGTTGGAG | ||
| + | AACCAACTGGCATTCTTGTGCCAACAGGGGTTCAGCCTTGAAAATGCTCTTTACGCCTTG | ||
| + | AGCGCTGTTGGGCACTTCACCTTGGGCTGTGTACTGGAGGAGCAGGAGCACCAGGTCGCA | ||
| + | AAGGAGGAGCGCGAAACGCCCACCACAGATAGTATGCCACCCCTGTTGAAGCAGGCTATC | ||
| + | GAGCTTTTTGATCGGCAGGGTGCTGAGCCTGCCTTTTTGTTCGGCCTTGAGCTTATAATT | ||
| + | TGCGGCCTGGAAAAACAACTCAAGTGCGAAAGCGGTGGTCCCACAGACGCATTGGACGAT | ||
| + | TTTGATCTTGACATGCTGCCAGCCGATGCTTTGGACGATTTTGACCTGGATATGTTGCCG | ||
| + | GCAGATGCCCTCGACGACTTTGATCTTGATATGTTGCCGGGCTAAggatccGGAGGT</pre> | ||
| + | <pre>> mN1ce(CO) | ||
| + | CCCTGCGTCGGTAGCAACCCCTGCTACAATCAAGGGACCTGCGAGCCAACGTCAGAAAAT | ||
| + | CCCTTTTATAGATGCCTGTGCCCGGCAAAGTTCAACGGACTGCTGTGCCACATACTCGAC | ||
| + | TACAGTTTTACTGGGGGGGCGGGCCGGGATATACCCCCGCCTCAGATAGAAGAGGCATGC | ||
| + | GAACTGCCGGAATGCCAGGTCGATGCGGGCAATAAAGTCTGCAACCTCCAATGCAATAAT | ||
| + | CACGCCTGCGGTTGGGACGGGGGTGACTGCTCACTTAACTTCAATGATCCGTGGAAGAAC | ||
| + | TGCACCCAGTCCCTCCAATGTTGGAAATATTTTTCCGATGGTCATTGTGATAGCCAGTGC | ||
| + | AATTCCGCAGGATGTTTGTTCGATGGGTTTGACTGCCAACTGACAGAGGGTCAGTGTAAT | ||
| + | CCTCTTTACGACCAATACTGCAAGGATCATTTCAGTGATGGACATTGTGACCAAGGTTGT | ||
| + | AACAGTGCGGAGTGCGAATGGGATGGGTTGGATTGTGCTGAGCATGTTCCTGAGCGCCTC | ||
| + | GCAGCGGGAACTTTGGTCCTCGTTGTCCTCCTTCCGCCTGACCAGCTCAGAAATAATAGC | ||
| + | TTTCACTTTCTTAGAGAACTCTCTCATGTGTTGCATACCAACGTAGTCTTTAAGCGCGAC | ||
| + | GCTCAAGGACAGCAAATGATCTTCCCGTACTATGGGCACGAAGAAGAGCTTCGGAAACAC | ||
| + | CCGATAAAAAGGAGTACGGTTGGTTGGGCGACGAGTAGTCTTTTGCCGGGCACGAGTGGT | ||
| + | GGCAGACAACGGCGGGAACTCGATCCGATGGACATACGGGGGTCCATAGTGTATCTGGAG | ||
| + | ATTGACAACCGACAGTGTGTTCAAAGTTCAAGCCAGTGTTTTCAATCAGCCACTGATGTA | ||
| + | GCTGCGTTTCTCGGAGCGTTGGCCTCTCTTGGGAGCTTGAATATTCCGTATAAAATCGAA | ||
| + | GCTGTAAAAAGCGAACCTGTCGAACCGCCATTGCCAAGCCAGTTGCACCTCATGTACGTG | ||
| + | GCCGCTGCTGCGTTCGTACTCTTGTTCTTCGTAGGCTGCGGTGTTTTGCTGTCACGAAAG | ||
| + | CGCCGACGG</pre> | ||
| + | <pre>> hN1c(CO) | ||
| + | CCCTGTCTTGGAGGGAATCCGTGTTACAACCAAGGGACATGTGAACCCACGAGCGAGTCT | ||
| + | CCTTTTTACAGGTGTCTCTGTCCAGCAAAATTTAATGGATTGCTTTGCCACATTCTGGAT | ||
| + | TACTCATTTGGTGGGGGAGCGGGGCGGGACATACCGCCGCCCCTTATTGAAGAAGCATGC | ||
| + | GAACTGCCCGAGTGCCAGGAGGATGCAGGTAACAAGGTATGCTCTCTTCAGTGCAATAAC | ||
| + | CACGCTTGCGGGTGGGATGGTGGCGATTGCTCCTTGAATTTTAATGATCCTTGGAAAAAT | ||
| + | TGCACCCAATCACTTCAGTGTTGGAAATACTTCTCAGATGGCCATTGCGATTCTCAGTGT | ||
| + | AACAGCGCGGGATGCTTGTTTGACGGATTCGACTGTCAAAGGGCTGAGGGACAATGTAAT | ||
| + | CCGCTCTACGATCAGTATTGTAAAGACCATTTCAGCGACGGCCACTGTGACCAAGGCTGC | ||
| + | AATAGTGCCGAGTGTGAGTGGGATGGGTTGGATTGCGCGGAGCATGTTCCGGAGAGACTT | ||
| + | GCGGCAGGTACGCTCGTGGTTGTTGTACTCATGCCGCCTGAGCAACTGAGGAATAGTAGC | ||
| + | TTTCACTTCCTCCGGGAACTGAGCCGGGTTCTGCATACTAACGTTGTATTTAAGAGAGAT | ||
| + | GCACATGGTCAGCAAATGATATTTCCGTACTACGGTCGGGAAGAGGAATTGCGGAAGCAC | ||
| + | CCCATTAAGAGAGCTGCCGAGGGTTGGGCCGCTCCGGATGCCCTCCTTGGACAAGTAAAA | ||
| + | GCCTCCCTCCTTCCCGGCGGATCTGAGGGTGGACGGAGACGCCGGGAACTCGATCCAATG | ||
| + | GATGTAAGAGGGTCCATTGTGTACTTGGAGATTGATAATCGCCAGTGTGTGCAGGCGTCC | ||
| + | TCACAATGTTTTCAAAGTGCGACCGATGTCGCAGCATTCTTGGGTGCCCTGGCATCATTG | ||
| + | GGCAGCCTGAATATTCCATATAAGATCGAAGCGGTGCAATCCGAAACGGTGGAACCGCCT | ||
| + | CCACCGGCACAGCTTCATTTCATGTATGTCGCAGCAGCAGCTTTCGTTTTGCTTTTCTTC | ||
| + | GTAGGGTGTGGTGTTCTCCTTAGTCGAAAACGACGCCGG</pre> | ||
| + | <pre>> LaG17(CO) | ||
| + | tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG | ||
| + | CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG | ||
| + | GAGGACCTCATGGCCGATGTGCAGCTCGTCGAGTCAGGTGGGGGTCTCGTACAAGCTGGG | ||
| + | GGGTCACTCAGACTGTCTTGCGCCGCCAGTGGCAGAACCATCTCTATGGCAGCTATGTCC | ||
| + | TGGTTCAGACAGGCGCCAGGCAAGGAGAGGGAGTTTGTGGCGGGTATAAGTAGGTCCGCA | ||
| + | GGAAGCGCGGTCCACGCCGATTCAGTCAAAGGTCGGTTCACGATAAGCCGGGATAACACT | ||
| + | AAGAACACTCTTTATCTCCAGATGAACTCTCTGAAGGCCGAGGATACGGCTGTTTACTAT | ||
| + | TGCGCTGTACGGACATCTGGGTTCTTCGGGAGCATACCGCGCACCGGAACAGCGTTTGAT | ||
| + | TACTGGGGACAAGGTACACAGGTGACCGTTTCCCCCTGCGTCGGTAGCAACCC</pre> | ||
| + | <pre>> LaG16-2(CO) | ||
| + | tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG | ||
| + | CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG | ||
| + | GAGGACCTCATGGCACAGGTCCAACTCGTCGAATCCGGAGGTCGCCTCGTGCAAGCTGGC | ||
| + | GATTCACTTCGACTCTCTTGCGCGGCGTCAGGTCGGACCTTCAGCACGTCAGCTATGGCG | ||
| + | TGGTTCCGCCAGGCGCCTGGGAGAGAGAGAGAGTTTGTAGCAGCCATCACATGGACTGTT | ||
| + | GGAAATACTATATTGGGCGACTCCGTAAAAGGGCGCTTCACTATCTCACGAGATAGAGCG | ||
| + | AAAAACACTGTAGATCTGCAAATGGACAATTTGGAGCCGGAGGATACTGCTGTGTATTAT | ||
| + | TGCAGCGCTCGCTCTCGGGGGTACGTCCTCTCCGTACTCCGGAGCGTGGATTCATACGAC | ||
| + | TACTGGGGACAGGGCACACAAGTAACGGTAAGCGGTGGGGGAGGGTCTGGTGGTGGAGGC | ||
| + | TCAGGAGGCGGGGGATCTATGGCTCAGGTCCAGCTTGTAGAGAGCGGTGGTGGATTGGTG | ||
| + | CAAGCAGGAGGGAGCCTCAGGCTTAGTTGTGCTGCCTCCGGTCGAACCTTCTCAAATTAT | ||
| + | GCAATGGGTTGGTTTAGGCAAGCTCCTGGTAAAGAACGGGAGTTCGTGGCCGCAATTAGC | ||
| + | TGGACGGGAGTTAGCACATATTACGCTGACTCTGTGAAAGGTCGGTTCACGATAAGTCGA | ||
| + | GACAATGATAAGAATACGGTTTACGTCCAAATGAATAGCCTCATCCCGGAGGACACAGCT | ||
| + | ATATATTATTGCGCTGCTGTACGGGCAAGATCCTTCTCAGATACTTATAGCAGGGTTAAC | ||
| + | GAATACGATTATTGGGGGCAGGGAACGCAAGTCACTGTTCCCTGCGTCGGTAGCAACCC</pre> | ||
| + | <pre>> EGFPtm(CO) | ||
| + | GGAAGAAAATCCCGGTCCGATGGCCCTTCCTGTAACGGCGCTCCTTCTCCCTCTTGCCC | ||
| + | TCCTGCTCCATGCCGCACGACCAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGG | ||
| + | TGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCG | ||
| + | AGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA | ||
| + | AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA | ||
| + | GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT | ||
| + | ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG | ||
| + | TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGG | ||
| + | AGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATA | ||
| + | TCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCG | ||
| + | AGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC | ||
| + | CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA | ||
| + | ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCG | ||
| + | GCATGGACGAGCTGTACAAGAATGCCGTTGGTCAAGACACGCAGGAAGTCATTGTTGTAC | ||
| + | CCCACTCCCTGCCATTTAAGGTGGTAGTAATTTCAGCCATCCTTGCTCTCGTTGTGTTGA | ||
| + | CAATTATTTCTCTGATAATTCTTATCATGCTCTGGCAAAAGAAACCTCGCTGAaagcttg | ||
| + | gaacccttaatataacttcgtat</pre> | ||
| + | <pre>> CD19ECD(CO) | ||
| + | TTGCTCCCACTGGCCTTGCTGCTTCATGCGGCACGGCCTTACCCCTATGATGTACCAGAT | ||
| + | TACGCGGAAGAGCCTCTCGTCGTGAAAGTTGAAGAAGGTGACAATGCAGTTTTGCAATGT | ||
| + | CTGAAAGGGACGTCTGACGGACCAACCCAACAGCTCACTTGGAGTCGCGAATCCCCGCTT | ||
| + | AAGCCGTTCCTGAAGTTGTCTCTGGGGTTGCCGGGTCTTGGTATACACATGCGCCCGCTT | ||
| + | GCGATATGGCTCTTCATCTTCAATGTCAGCCAACAGATGGGCGGGTTCTATCTCTGTCAA | ||
| + | CCGGGACCGCCATCAGAGAAGGCGTGGCAACCTGGTTGGACGGTAAACGTGGAAGGTAGC | ||
| + | GGGGAGTTGTTTCGCTGGAACGTTTCTGATCTCGGGGGCCTGGGTTGCGGTCTGAAAAAT | ||
| + | CGCTCTAGCGAAGGGCCGAGTTCACCTTCCGGCAAGCTCATGTCCCCGAAACTCTACGTG | ||
| + | TGGGCGAAGGATAGGCCAGAAATTTGGGAGGGAGAACCCCCCTGCCTGCCGCCCAGAGAT | ||
| + | TCACTCAACCAGAGTCTCTCCCAAGATCTGACTATGGCCCCAGGAAGTACCTTGTGGCTT | ||
| + | AGCTGCGGTGTACCGCCCGACTCCGTGAGCCGAGGGCCTCTCTCTTGGACACACGTTCAC | ||
| + | CCTAAGGGGCCGAAATCATTGTTGAGTTTGGAACTCAAAGACGATAGGCCAGCAAGGGAT | ||
| + | ATGTGGGTTATGGAGACCGGCCTTCTGCTCCCACGAGCGACCGCTCAGGATGCGGGCAAG | ||
| + | TATTATTGCCACAGAGGAAATCTGACTATGTCTTTTCATCTGGAAATTACTGCGCGGCCA | ||
| + | GTTTTGTGGCACTGGCTGTTGCGCACGGGGGGATGGAAGGAGCAGAAATTGATCTCTGA</pre> | ||
| + | <pre>> Gal4DBD(CO) | ||
| + | atgaagctgctgagcagcatcgagcaggcctgtgacatctgccggctgaagaaactgaag | ||
| + | tgcagcaaagaaaagcccaagtgcgccaagtgcctgaagaacaactgggagtgccggtac | ||
| + | agccccaagaccaagagaagccccctgaccagagcccacctgaccgaggtggaaagccgg | ||
| + | ctggaaagactggaacagctgtttctgctgatcttcccacgcgaggacctggacatgatc | ||
| + | ctgaagatggacagcctccaggacatcaaggccctgctgaccggcctgttcgtgcaggac | ||
| + | aacgtgaacaaggacgccgtgaccgacagactggccagcgtggaaaccgacatgcccctg | ||
| + | accctgcggcagcacagaatcagcgccaccagcagcagcgaggaaagcagcaacaagggc | ||
| + | cagcggcagctgacagtgtct</pre> | ||
| + | <pre>> ZF21.16(CO) | ||
| + | GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCTCCTCACGAGAGGCCTTTTCAATGT | ||
| + | AGAATTTGTATGCGTAATTTTTCTaggaacttcatcctccagagaCATACAAGGACTCAC | ||
| + | ACAGGTGAAAAGCCTTTTCAGTGCAGAATTTGTATGCGCAATTTTTCTcagggcggtaac | ||
| + | ctggtgagaCATCTGCGTACCCACACTGGTGAAAAGCCTTTCCAATGTAGAATTTGTATG | ||
| + | CGAAATTTTTCTcagcaaaccggcctgaacgtgCATCTGAAGACACACACAGGCGAGAAG | ||
| + | GGATCC</pre> | ||
| + | <pre>> ZF42.10(CO) | ||
| + | GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCACCACATGAAAGACCATTCCAATGT | ||
| + | AGAATTTGTATGAGGAATTTTTCTaccggccagatcctggacagaCATACAAGGACCCAC | ||
| + | ACCGGCGAAAAGCCATTCCAGTGTAGAATTTGTATGCGTAATTTTTCTgtggcccacagc | ||
| + | ctgaagagaCATCTGAGGACCCACACCGGAGAGAAGCCTTTTCAATGCAGAATTTGTATG | ||
| + | CGTAATTTTTCTgaccccagcaacctgagaaggCATCTGAAGACCCACACCGGAGAAAAG | ||
| + | GGATCC</pre> | ||
| + | <pre>> ZF43.8(CO) | ||
| + | GCTAGCCCCAGAGTGAGAACCGGTTCTAAGACACCTCCCCACGAGAGGCCTTTTCAGTGT | ||
| + | AGAATTTGTATGCGTAATTTTTCTaggcaggacagactggacaggCACACAAGGACTCAC | ||
| + | ACTGGTGAAAAGCCCTTCCAATGTAGAATTTGTATGCGCAATTTTTCTcagaaggagcac | ||
| + | ctggccggcCATCTGAGGACCCACACAGGCGAAAAGCCTTTCCAGTGCAGAATTTGTATG | ||
| + | AGAAATTTTTCTaggagagacaacctgaacagaCATCTGAAGACACATACAGGTGAAAAA | ||
| + | GGATCC</pre> | ||
| + | <pre>> TEVp(FL)(CO) | ||
| + | ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT | ||
| + | TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC | ||
| + | TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA | ||
| + | AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA | ||
| + | ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT | ||
| + | TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT | ||
| + | TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC | ||
| + | ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG | ||
| + | TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT | ||
| + | AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG | ||
| + | ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC | ||
| + | TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG | ||
| + | GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC</pre> | ||
| + | <pre>> ZF21_16N-CfaN-CfaC-ZF21_16C(CO) | ||
| + | ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG | ||
| + | ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC | ||
| + | TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA | ||
| + | TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA | ||
| + | GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG | ||
| + | ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG | ||
| + | TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG | ||
| + | ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT | ||
| + | AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG | ||
| + | CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA | ||
| + | AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA | ||
| + | TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG | ||
| + | AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC</pre> | ||
| + | </div> | ||
</main> | </main> | ||
</div> | </div> | ||
| + | |||
<!--Abstract on content page--> | <!--Abstract on content page--> | ||
<div id="abstractContent" class="z-depth-2"> | <div id="abstractContent" class="z-depth-2"> | ||
| − | <a href="#!"><img src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> | + | <a href="#!"><img alt="2018 team Fudan abstract" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> |
<div class="container"> | <div class="container"> | ||
<h2 style="margin: 0;padding: 10px 0;">Abstract</h2> | <h2 style="margin: 0;padding: 10px 0;">Abstract</h2> | ||
| − | <p style="margin:0 | + | <p style="margin: 0">Contact-dependent signaling is critical for multicellular biological |
events, yet customizing contact-dependent signal transduction between | events, yet customizing contact-dependent signal transduction between | ||
cells remains challenging. Here we have developed the ENABLE toolbox, a | cells remains challenging. Here we have developed the ENABLE toolbox, a | ||
| Line 319: | Line 1,297: | ||
</p> | </p> | ||
</div> | </div> | ||
| + | </div> | ||
| + | |||
| + | <!-- Floating Btns --> | ||
| + | <div class="floatingBtn"> | ||
| + | <a href="#!" id="abstractBtn" class="btn"> | ||
| + | <i class="fa fa-sticky-note" style="font-size: 30px;line-height: 50px"></i> | ||
| + | </a> | ||
| + | <a href="#FudanWrapper" class="btn"> | ||
| + | <i class="fa fa-angle-up" style="font-size: 48px;line-height: 45px"></i> | ||
| + | </a> | ||
</div> | </div> | ||
| Line 326: | Line 1,314: | ||
<div class="row"> | <div class="row"> | ||
<div id="sponsor" class="col m3 s12 row"> | <div id="sponsor" class="col m3 s12 row"> | ||
| − | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> | + | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> |
</a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | ||
</a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | ||
| Line 338: | Line 1,326: | ||
<span>Project</span> | <span>Project</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | + | ||
| − | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
<li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | ||
</ul> | </ul> | ||
| Line 348: | Line 1,336: | ||
<span>Dry lab</span> | <span>Dry lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> | |
| − | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> | |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="col s12 m4"> | + | <div class="col s12 m4 active"> |
<span>Wet lab</span> | <span>Wet lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 371: | Line 1,359: | ||
<span>Toolbox</span> | <span>Toolbox</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 382: | Line 1,370: | ||
<span>Outreach</span> | <span>Outreach</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| − | <div class="col s12 m4 | + | <div class="col s12 m4"> |
<span>Team</span> | <span>Team</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 405: | Line 1,393: | ||
<div class="footer-copyright"> | <div class="footer-copyright"> | ||
<div class="container"> | <div class="container"> | ||
| − | + | <div class="contactUS row"> | |
| − | + | <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg E301, 2005 Songhu Rd, Shanghai | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn | |
| − | + | </div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM | |
| − | + | </div> | |
| − | + | </div> | |
| − | + | </div> | |
</div> | </div> | ||
</footer> | </footer> | ||
| Line 427: | Line 1,415: | ||
<!-- Javascript --> | <!-- Javascript --> | ||
<script src="https://2018.igem.org/wiki/index.php?title=Template:Fudan/js.js&action=raw&ctype=text/javascript"></script> | <script src="https://2018.igem.org/wiki/index.php?title=Template:Fudan/js.js&action=raw&ctype=text/javascript"></script> | ||
| + | |||
</body> | </body> | ||
</html> | </html> | ||
Latest revision as of 15:20, 7 November 2018
- Addon: ribo
- Addon: TALE
- Addon: T2
- Model: transcriptional amplifer
- Model: Notch-ligand kinetics
- Software
Primers used
Primers
gBlocks
> alphaCD19(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCTACCCCTATGATGTACCAGAT TACGCGGATATACAAATGACCCAGACTACGTCCAGCCTTTCCGCCTCCCTGGGTGATCGC GTGACAATCAGTTGTAGGGCTAGTCAGGACATAAGTAAATACCTCAACTGGTATCAACAG AAGCCTGACGGAACAGTTAAACTTCTTATCTATCATACAAGTAGGCTTCATTCAGGCGTT CCATCCAGATTTAGTGGATCTGGGAGCGGCACCGACTACTCACTCACGATTAGTAATCTG GAACAAGAGGACATCGCCACTTATTTTTGCCAGCAGGGTAACACTCTTCCCTATACATTC GGCGGCGGTACGAAACTCGAAATTACAGGGGGGGGTGGCAGTGGTGGCGGAGGTTCCGGT GGGGGTGGTTCAGAAGTAAAACTTCAGGAATCTGGTCCAGGGCTGGTTGCACCCAGCCAA TCCCTTTCCGTCACGTGCACCGTATCCGGGGTCTCCCTCCCAGACTACGGCGTGAGTTGG ATACGACAGCCGCCGCGCAAGGGGCTTGAGTGGCTGGGTGTCATTTGGGGATCTGAAACA ACGTATTATAATTCCGCTTTGAAATCCCGCCTCACTATTATCAAGGACAATTCTAAGAGC CAGGTCTTTCTGAAGATGAACAGCCTGCAAACGGATGACACAGCCATCTACTATTGTGCA AAGCACTATTACTATGGTGGTTCATACGCTATGGATTACTGGGGACAAGGGACATCCGTA ACCGTTTCATCACCCTGCGTCGGTAGCAACCC
> tTAA(CO) TGTCACGAAAGCGCCGACGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAGCGCAC TGGAGCTTCTGAACGAAGTAGGTATCGAGGGGCTCACCACACGAAAATTGGCACAGAAGC TCGGAGTGGAGCAGCCGACTTTGTACTGGCACGTTAAGAATAAGAGGGCTTTGCTTGACG CGCTGGCCATTGAGATGCTCGACCGGCATCACACTCACTTCTGCCCGCTTGAAGGCGAGA GTTGGCAAGATTTCCTTAGGAACAATGCAAAGAGCTTTCGATGCGCCCTGCTTAGCCATC GGGACGGCGCAAAGGTTCATCTTGGGACTCGGCCTACTGAGAAGCAATATGAAACCCTGG AAAACCAGCTTGCTTTCCTTTGCCAACAGGGGTTCTCACTGGAGAATGCCCTGTACGCAC TCTCCGCGGTCGGCCATTTTACTCTCGGGTGTGTTTTGGAAGATCAGGAACACCAGGTAG CGAAAGAGGAGCGAGAAACGCCTACAACTGATTCCATGCCACCGTTGCTGAGACAAGCTA TAGAGTTGTTCGACCACCAGGGTGCTGAACCGGCGTTTCTCTTTGGTCTGGAACTCATTA TCTGCGGCCTTGAAAAGCAGCTTAAGTGCGAGAGTGGCGGTCCCGCAGATGCACTTGATG ACTTTGATTTGGATATGTTGCCCGCGGACGCTCTTGATGACTTCGATCTGGATATGCTGC CGGCTGATGCCCTCGACGATCTTGACCTGGATATGCTCCCAGGATGAaagcttggaaccc ttaatataacttcgtat
> TetOn3G(CO) GGAGGTgaattcGCCACCATGAGCCGACTGGACAAGAGCAAGGTAATCAATTCAGCTTTG GAGCTTCTGAACGGCGTGGGAATCGAGGGTCTCACCACCAGAAAGCTCGCGCAAAAATTG GGGGTGGAACAACCGACTCTCTATTGGCATGTTAAGAACAAGAGGGCCCTCCTTGACGCA CTCCCCATCGAAATGCTGGATCGACATCACACACATAGTTGTCCGCTGGAGGGGGAGTCT TGGCAAGATTTCCTCAGGAATAACGCGAAGAGTTATCGGTGCGCTCTCCTTAGTCATCGC GATGGAGCGAAAGTGCATCTGGGGACAAGACCGACGGAAAAACAATATGAGACGTTGGAG AACCAACTGGCATTCTTGTGCCAACAGGGGTTCAGCCTTGAAAATGCTCTTTACGCCTTG AGCGCTGTTGGGCACTTCACCTTGGGCTGTGTACTGGAGGAGCAGGAGCACCAGGTCGCA AAGGAGGAGCGCGAAACGCCCACCACAGATAGTATGCCACCCCTGTTGAAGCAGGCTATC GAGCTTTTTGATCGGCAGGGTGCTGAGCCTGCCTTTTTGTTCGGCCTTGAGCTTATAATT TGCGGCCTGGAAAAACAACTCAAGTGCGAAAGCGGTGGTCCCACAGACGCATTGGACGAT TTTGATCTTGACATGCTGCCAGCCGATGCTTTGGACGATTTTGACCTGGATATGTTGCCG GCAGATGCCCTCGACGACTTTGATCTTGATATGTTGCCGGGCTAAggatccGGAGGT
> mN1ce(CO) CCCTGCGTCGGTAGCAACCCCTGCTACAATCAAGGGACCTGCGAGCCAACGTCAGAAAAT CCCTTTTATAGATGCCTGTGCCCGGCAAAGTTCAACGGACTGCTGTGCCACATACTCGAC TACAGTTTTACTGGGGGGGCGGGCCGGGATATACCCCCGCCTCAGATAGAAGAGGCATGC GAACTGCCGGAATGCCAGGTCGATGCGGGCAATAAAGTCTGCAACCTCCAATGCAATAAT CACGCCTGCGGTTGGGACGGGGGTGACTGCTCACTTAACTTCAATGATCCGTGGAAGAAC TGCACCCAGTCCCTCCAATGTTGGAAATATTTTTCCGATGGTCATTGTGATAGCCAGTGC AATTCCGCAGGATGTTTGTTCGATGGGTTTGACTGCCAACTGACAGAGGGTCAGTGTAAT CCTCTTTACGACCAATACTGCAAGGATCATTTCAGTGATGGACATTGTGACCAAGGTTGT AACAGTGCGGAGTGCGAATGGGATGGGTTGGATTGTGCTGAGCATGTTCCTGAGCGCCTC GCAGCGGGAACTTTGGTCCTCGTTGTCCTCCTTCCGCCTGACCAGCTCAGAAATAATAGC TTTCACTTTCTTAGAGAACTCTCTCATGTGTTGCATACCAACGTAGTCTTTAAGCGCGAC GCTCAAGGACAGCAAATGATCTTCCCGTACTATGGGCACGAAGAAGAGCTTCGGAAACAC CCGATAAAAAGGAGTACGGTTGGTTGGGCGACGAGTAGTCTTTTGCCGGGCACGAGTGGT GGCAGACAACGGCGGGAACTCGATCCGATGGACATACGGGGGTCCATAGTGTATCTGGAG ATTGACAACCGACAGTGTGTTCAAAGTTCAAGCCAGTGTTTTCAATCAGCCACTGATGTA GCTGCGTTTCTCGGAGCGTTGGCCTCTCTTGGGAGCTTGAATATTCCGTATAAAATCGAA GCTGTAAAAAGCGAACCTGTCGAACCGCCATTGCCAAGCCAGTTGCACCTCATGTACGTG GCCGCTGCTGCGTTCGTACTCTTGTTCTTCGTAGGCTGCGGTGTTTTGCTGTCACGAAAG CGCCGACGG
> hN1c(CO) CCCTGTCTTGGAGGGAATCCGTGTTACAACCAAGGGACATGTGAACCCACGAGCGAGTCT CCTTTTTACAGGTGTCTCTGTCCAGCAAAATTTAATGGATTGCTTTGCCACATTCTGGAT TACTCATTTGGTGGGGGAGCGGGGCGGGACATACCGCCGCCCCTTATTGAAGAAGCATGC GAACTGCCCGAGTGCCAGGAGGATGCAGGTAACAAGGTATGCTCTCTTCAGTGCAATAAC CACGCTTGCGGGTGGGATGGTGGCGATTGCTCCTTGAATTTTAATGATCCTTGGAAAAAT TGCACCCAATCACTTCAGTGTTGGAAATACTTCTCAGATGGCCATTGCGATTCTCAGTGT AACAGCGCGGGATGCTTGTTTGACGGATTCGACTGTCAAAGGGCTGAGGGACAATGTAAT CCGCTCTACGATCAGTATTGTAAAGACCATTTCAGCGACGGCCACTGTGACCAAGGCTGC AATAGTGCCGAGTGTGAGTGGGATGGGTTGGATTGCGCGGAGCATGTTCCGGAGAGACTT GCGGCAGGTACGCTCGTGGTTGTTGTACTCATGCCGCCTGAGCAACTGAGGAATAGTAGC TTTCACTTCCTCCGGGAACTGAGCCGGGTTCTGCATACTAACGTTGTATTTAAGAGAGAT GCACATGGTCAGCAAATGATATTTCCGTACTACGGTCGGGAAGAGGAATTGCGGAAGCAC CCCATTAAGAGAGCTGCCGAGGGTTGGGCCGCTCCGGATGCCCTCCTTGGACAAGTAAAA GCCTCCCTCCTTCCCGGCGGATCTGAGGGTGGACGGAGACGCCGGGAACTCGATCCAATG GATGTAAGAGGGTCCATTGTGTACTTGGAGATTGATAATCGCCAGTGTGTGCAGGCGTCC TCACAATGTTTTCAAAGTGCGACCGATGTCGCAGCATTCTTGGGTGCCCTGGCATCATTG GGCAGCCTGAATATTCCATATAAGATCGAAGCGGTGCAATCCGAAACGGTGGAACCGCCT CCACCGGCACAGCTTCATTTCATGTATGTCGCAGCAGCAGCTTTCGTTTTGCTTTTCTTC GTAGGGTGTGGTGTTCTCCTTAGTCGAAAACGACGCCGG
> LaG17(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG GAGGACCTCATGGCCGATGTGCAGCTCGTCGAGTCAGGTGGGGGTCTCGTACAAGCTGGG GGGTCACTCAGACTGTCTTGCGCCGCCAGTGGCAGAACCATCTCTATGGCAGCTATGTCC TGGTTCAGACAGGCGCCAGGCAAGGAGAGGGAGTTTGTGGCGGGTATAAGTAGGTCCGCA GGAAGCGCGGTCCACGCCGATTCAGTCAAAGGTCGGTTCACGATAAGCCGGGATAACACT AAGAACACTCTTTATCTCCAGATGAACTCTCTGAAGGCCGAGGATACGGCTGTTTACTAT TGCGCTGTACGGACATCTGGGTTCTTCGGGAGCATACCGCGCACCGGAACAGCGTTTGAT TACTGGGGACAAGGTACACAGGTGACCGTTTCCCCCTGCGTCGGTAGCAACCC
> LaG16-2(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG GAGGACCTCATGGCACAGGTCCAACTCGTCGAATCCGGAGGTCGCCTCGTGCAAGCTGGC GATTCACTTCGACTCTCTTGCGCGGCGTCAGGTCGGACCTTCAGCACGTCAGCTATGGCG TGGTTCCGCCAGGCGCCTGGGAGAGAGAGAGAGTTTGTAGCAGCCATCACATGGACTGTT GGAAATACTATATTGGGCGACTCCGTAAAAGGGCGCTTCACTATCTCACGAGATAGAGCG AAAAACACTGTAGATCTGCAAATGGACAATTTGGAGCCGGAGGATACTGCTGTGTATTAT TGCAGCGCTCGCTCTCGGGGGTACGTCCTCTCCGTACTCCGGAGCGTGGATTCATACGAC TACTGGGGACAGGGCACACAAGTAACGGTAAGCGGTGGGGGAGGGTCTGGTGGTGGAGGC TCAGGAGGCGGGGGATCTATGGCTCAGGTCCAGCTTGTAGAGAGCGGTGGTGGATTGGTG CAAGCAGGAGGGAGCCTCAGGCTTAGTTGTGCTGCCTCCGGTCGAACCTTCTCAAATTAT GCAATGGGTTGGTTTAGGCAAGCTCCTGGTAAAGAACGGGAGTTCGTGGCCGCAATTAGC TGGACGGGAGTTAGCACATATTACGCTGACTCTGTGAAAGGTCGGTTCACGATAAGTCGA GACAATGATAAGAATACGGTTTACGTCCAAATGAATAGCCTCATCCCGGAGGACACAGCT ATATATTATTGCGCTGCTGTACGGGCAAGATCCTTCTCAGATACTTATAGCAGGGTTAAC GAATACGATTATTGGGGGCAGGGAACGCAAGTCACTGTTCCCTGCGTCGGTAGCAACCC
> EGFPtm(CO) GGAAGAAAATCCCGGTCCGATGGCCCTTCCTGTAACGGCGCTCCTTCTCCCTCTTGCCC TCCTGCTCCATGCCGCACGACCAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGG TGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCG AGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGG AGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATA TCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCG AGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCG GCATGGACGAGCTGTACAAGAATGCCGTTGGTCAAGACACGCAGGAAGTCATTGTTGTAC CCCACTCCCTGCCATTTAAGGTGGTAGTAATTTCAGCCATCCTTGCTCTCGTTGTGTTGA CAATTATTTCTCTGATAATTCTTATCATGCTCTGGCAAAAGAAACCTCGCTGAaagcttg gaacccttaatataacttcgtat
> CD19ECD(CO) TTGCTCCCACTGGCCTTGCTGCTTCATGCGGCACGGCCTTACCCCTATGATGTACCAGAT TACGCGGAAGAGCCTCTCGTCGTGAAAGTTGAAGAAGGTGACAATGCAGTTTTGCAATGT CTGAAAGGGACGTCTGACGGACCAACCCAACAGCTCACTTGGAGTCGCGAATCCCCGCTT AAGCCGTTCCTGAAGTTGTCTCTGGGGTTGCCGGGTCTTGGTATACACATGCGCCCGCTT GCGATATGGCTCTTCATCTTCAATGTCAGCCAACAGATGGGCGGGTTCTATCTCTGTCAA CCGGGACCGCCATCAGAGAAGGCGTGGCAACCTGGTTGGACGGTAAACGTGGAAGGTAGC GGGGAGTTGTTTCGCTGGAACGTTTCTGATCTCGGGGGCCTGGGTTGCGGTCTGAAAAAT CGCTCTAGCGAAGGGCCGAGTTCACCTTCCGGCAAGCTCATGTCCCCGAAACTCTACGTG TGGGCGAAGGATAGGCCAGAAATTTGGGAGGGAGAACCCCCCTGCCTGCCGCCCAGAGAT TCACTCAACCAGAGTCTCTCCCAAGATCTGACTATGGCCCCAGGAAGTACCTTGTGGCTT AGCTGCGGTGTACCGCCCGACTCCGTGAGCCGAGGGCCTCTCTCTTGGACACACGTTCAC CCTAAGGGGCCGAAATCATTGTTGAGTTTGGAACTCAAAGACGATAGGCCAGCAAGGGAT ATGTGGGTTATGGAGACCGGCCTTCTGCTCCCACGAGCGACCGCTCAGGATGCGGGCAAG TATTATTGCCACAGAGGAAATCTGACTATGTCTTTTCATCTGGAAATTACTGCGCGGCCA GTTTTGTGGCACTGGCTGTTGCGCACGGGGGGATGGAAGGAGCAGAAATTGATCTCTGA
> Gal4DBD(CO) atgaagctgctgagcagcatcgagcaggcctgtgacatctgccggctgaagaaactgaag tgcagcaaagaaaagcccaagtgcgccaagtgcctgaagaacaactgggagtgccggtac agccccaagaccaagagaagccccctgaccagagcccacctgaccgaggtggaaagccgg ctggaaagactggaacagctgtttctgctgatcttcccacgcgaggacctggacatgatc ctgaagatggacagcctccaggacatcaaggccctgctgaccggcctgttcgtgcaggac aacgtgaacaaggacgccgtgaccgacagactggccagcgtggaaaccgacatgcccctg accctgcggcagcacagaatcagcgccaccagcagcagcgaggaaagcagcaacaagggc cagcggcagctgacagtgtct
> ZF21.16(CO) GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCTCCTCACGAGAGGCCTTTTCAATGT AGAATTTGTATGCGTAATTTTTCTaggaacttcatcctccagagaCATACAAGGACTCAC ACAGGTGAAAAGCCTTTTCAGTGCAGAATTTGTATGCGCAATTTTTCTcagggcggtaac ctggtgagaCATCTGCGTACCCACACTGGTGAAAAGCCTTTCCAATGTAGAATTTGTATG CGAAATTTTTCTcagcaaaccggcctgaacgtgCATCTGAAGACACACACAGGCGAGAAG GGATCC
> ZF42.10(CO) GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCACCACATGAAAGACCATTCCAATGT AGAATTTGTATGAGGAATTTTTCTaccggccagatcctggacagaCATACAAGGACCCAC ACCGGCGAAAAGCCATTCCAGTGTAGAATTTGTATGCGTAATTTTTCTgtggcccacagc ctgaagagaCATCTGAGGACCCACACCGGAGAGAAGCCTTTTCAATGCAGAATTTGTATG CGTAATTTTTCTgaccccagcaacctgagaaggCATCTGAAGACCCACACCGGAGAAAAG GGATCC
> ZF43.8(CO) GCTAGCCCCAGAGTGAGAACCGGTTCTAAGACACCTCCCCACGAGAGGCCTTTTCAGTGT AGAATTTGTATGCGTAATTTTTCTaggcaggacagactggacaggCACACAAGGACTCAC ACTGGTGAAAAGCCCTTCCAATGTAGAATTTGTATGCGCAATTTTTCTcagaaggagcac ctggccggcCATCTGAGGACCCACACAGGCGAAAAGCCTTTCCAGTGCAGAATTTGTATG AGAAATTTTTCTaggagagacaacctgaacagaCATCTGAAGACACATACAGGTGAAAAA GGATCC
> TEVp(FL)(CO) ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC
> ZF21_16N-CfaN-CfaC-ZF21_16C(CO) ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.