Difference between revisions of "Team:Fudan/Primers"

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We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2.
 
We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2.
 
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     <!-- CSS -->
 
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     <link rel="stylesheet" type="text/css" href="https://2018.igem.org/wiki/index.php?title=Template:Fudan/css.css&action=raw&ctype=text/css" />
 
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     <link href="https://2018.igem.org/wiki/index.php?title=Template:Fudan/font-awesome.css&action=raw&ctype=text/css" rel="stylesheet" />
  
 
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    <title>2018 iGEM Team:Fudan - Primers</title>
 
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</head>
  
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                 <div class="nav-wrapper">
 
                 <div class="nav-wrapper">
 
                     <div id="teamLogo" class="brand-logo">
 
                     <div id="teamLogo" class="brand-logo">
−
                         <a href="https://2018.igem.org/Team:Fudan" target="_self"><img src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a>
+
                         <a href="https://2018.igem.org/Team:Fudan" target="_self"><img alt="2018 team Fudan logo" src="https://static.igem.org/mediawiki/2018/c/c4/T--Fudan--teamLogo.svg"></a>
 
                     </div>
 
                     </div>
 
                     <ul id="nav-mobile" class="right blueBorder">
 
                     <ul id="nav-mobile" class="right blueBorder">
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             <!-- Dropdown and List elements in navigation bar -->
 
             <!-- Dropdown and List elements in navigation bar -->
 
             <ul id="dropdown1" class="dropdown-content">
 
             <ul id="dropdown1" class="dropdown-content">
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Demonstrate">Demonstration</a></li>
+
                 <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li>
  
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li>
+
                 <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Results">Transmembrane logic</a></li>
+
                 <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li>
 
             </ul>
 
             </ul>
 
             <ul id="dropdown2" class="dropdown-content">
 
             <ul id="dropdown2" class="dropdown-content">
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Addon#ribo">Addon: ribo</a></li>
+
                 <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Addon#TALE">Addon: TALE</a></li>
+
                 <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Addon#T2">Addon: T2</a></li>
+
                 <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
+
                 <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Model#War_Predictor">Model: war&nbsp;predictor</a></li>
+
                 <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand&nbsp;kinetics</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Software">Software</a></li>
+
                 <li><a href="/Team:Fudan/Software">Software</a></li>
 
             </ul>
 
             </ul>
 
             <ul id="dropdown3" class="dropdown-content">
 
             <ul id="dropdown3" class="dropdown-content">
−
                 <li><a href="https://2018.igem.org/Team:Fudan/InterLab">iGEM interLab</a></li>
+
                 <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Notebook">Our notebook</a></li>
+
                 <li><a href="/Team:Fudan/Notebook">Our notebook</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Primers">Primers used</a></li>
+
                 <li><a href="/Team:Fudan/Primers">Primers used</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Protocols">Protocols</a></li>
+
                 <li><a href="/Team:Fudan/Protocols">Protocols</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Safety">Safety</a></li>
+
                 <li><a href="/Team:Fudan/Safety">Safety</a></li>
 
             </ul>
 
             </ul>
 
             <ul id="dropdown4" class="dropdown-content">
 
             <ul id="dropdown4" class="dropdown-content">
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Basic_Parts">Basic parts</a></li>
+
                 <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Composite_Parts">Composite parts</a></li>
+
                 <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Optimization">Optimization</a></li>
+
                 <li><a href="/Team:Fudan/Optimization">Optimization</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Parts_Collection">Parts collection</a></li>
+
                 <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Improve">Parts improvement</a></li>
+
                 <li><a href="/Team:Fudan/Improve">Parts improvement</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Measurement">Quantification</a></li>
+
                 <li><a href="/Team:Fudan/Measurement">Quantification</a></li>
 
             </ul>
 
             </ul>
 
             <ul id="dropdown5" class="dropdown-content">
 
             <ul id="dropdown5" class="dropdown-content">
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Bio-Art">Bio-Art display</a></li>
+
                 <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Collaborations">Collaborations</a></li>
+
                 <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Design_Intention">Design intention</a></li>
+
                 <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Human_Practices">Human practices</a></li>
+
                 <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Public_Engagement">Public engagement</a></li>
+
                 <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li>
 
             </ul>
 
             </ul>
 
             <ul id="dropdown6" class="dropdown-content">
 
             <ul id="dropdown6" class="dropdown-content">
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
+
                 <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Attributions">Attributions</a></li>
+
                 <li><a href="/Team:Fudan/Attributions">Attributions</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Heritage">Heritage</a></li>
+
                 <li><a href="/Team:Fudan/Heritage">Heritage</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Team">Members</a></li>
+
                 <li><a href="/Team:Fudan/Team">Members</a></li>
−
                 <li><a href="https://2018.igem.org/Team:Fudan/Sponsors">Sponsors</a></li>
+
                 <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li>
 
             </ul>
 
             </ul>
  
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                             <div class="collapsible-body">
 
                             <div class="collapsible-body">
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Demonstrate">Demonstration</a></li>
+
                                     <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li>
  
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li>
+
                                     <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Results">Transmembrane logic</a></li>
+
                                     <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </div>
 
                             </div>
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                             <div class="collapsible-body">
 
                             <div class="collapsible-body">
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Addon#ribo">Addon: ribo</a></li>
+
                                     <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Addon#TALE">Addon: TALE</a></li>
+
                                     <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Addon#T2">Addon: T2</a></li>
+
                                     <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
+
                                     <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Model#War_Predictor">Model: war&nbsp;predictor</a></li>
+
                                     <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand&nbsp;kinetics</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Software">Software</a></li>
+
                                     <li><a href="/Team:Fudan/Software">Software</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </div>
 
                             </div>
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                             <div class="collapsible-body">
 
                             <div class="collapsible-body">
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/InterLab">iGEM interLab</a></li>
+
                                     <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Notebook">Our notebook</a></li>
+
                                     <li><a href="/Team:Fudan/Notebook">Our notebook</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Primers">Primers used</a></li>
+
                                     <li><a href="/Team:Fudan/Primers">Primers used</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Protocols">Protocols</a></li>
+
                                     <li><a href="/Team:Fudan/Protocols">Protocols</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Safety">Safety</a></li>
+
                                     <li><a href="/Team:Fudan/Safety">Safety</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </div>
 
                             </div>
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                             <div class="collapsible-body">
 
                             <div class="collapsible-body">
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Basic_Parts">Basic parts</a></li>
+
                                     <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Composite_Parts">Composite parts</a></li>
+
                                     <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Optimization">Optimization</a></li>
+
                                     <li><a href="/Team:Fudan/Optimization">Optimization</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Parts_Collection">Parts collection</a></li>
+
                                     <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Improve">Parts improvement</a></li>
+
                                     <li><a href="/Team:Fudan/Improve">Parts improvement</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Measurement">Quantification</a></li>
+
                                     <li><a href="/Team:Fudan/Measurement">Quantification</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </div>
 
                             </div>
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                             <div class="collapsible-body">
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Bio-Art">Bio-Art display</a></li>
+
                                     <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Collaborations">Collaborations</a></li>
+
                                     <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Design_Intention">Design intention</a></li>
+
                                     <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Human_Practices">Human practices</a></li>
+
                                     <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Public_Engagement">Public engagement</a></li>
+
                                     <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </div>
 
                             </div>
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                             <div class="collapsible-body">
 
                                 <ul>
 
                                 <ul>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
+
                                     <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Attributions">Attributions</a></li>
+
                                     <li><a href="/Team:Fudan/Attributions">Attributions</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Heritage">Heritage</a></li>
+
                                     <li><a href="/Team:Fudan/Heritage">Heritage</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Team">Members</a></li>
+
                                     <li><a href="/Team:Fudan/Team">Members</a></li>
−
                                     <li><a href="https://2018.igem.org/Team:Fudan/Sponsors">Sponsors</a></li>
+
                                     <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li>
 
                                 </ul>
 
                                 </ul>
 
                             </div>
 
                             </div>
Line 232: Line 234:
 
                     </div>
 
                     </div>
 
                     <div class="col s12 m6 valign-wrapper hide-on-med-and-up">
 
                     <div class="col s12 m6 valign-wrapper hide-on-med-and-up">
−
                         <span>... they are a lot of $$$</span>
+
                         <span>Here we present a library of the vast collection of primers we have designed</span>
 
                     </div>
 
                     </div>
 
                 </div>
 
                 </div>
 
                 <div id="figureBannerTitle" class="hide-on-small-only">
 
                 <div id="figureBannerTitle" class="hide-on-small-only">
 
                     <h1>Primers used</h1>
 
                     <h1>Primers used</h1>
−
                     <p><span>... they are a lot of $$$</span></p>
+
                     <p><span>Here we present a library of the vast collection of primers we have designed</span></p>
 
                 </div>
 
                 </div>
 
                 <div class="hide-on-small-only">
 
                 <div class="hide-on-small-only">
Line 271: Line 273:
 
<div class="section container">
 
<div class="section container">
 
<h2>Primers</h2>
 
<h2>Primers</h2>
−
                     <div class="tableHolder" style="overflow:hidden;">
+
                     <div class="tableHolder" style="overflow:hidden">
 
                             <table>
 
                             <table>
 
<tr><th>#</th><th>Oligo Name</th><th>DNA Sequence (5 to 3)</th></tr>
 
<tr><th>#</th><th>Oligo Name</th><th>DNA Sequence (5 to 3)</th></tr>
Line 288: Line 290:
 
<tr><td>O13</td><td>mNotch 1 core-PB</td><td>CCGCCGGCGCTTG</td></tr>
 
<tr><td>O13</td><td>mNotch 1 core-PB</td><td>CCGCCGGCGCTTG</td></tr>
 
<tr><td>O14</td><td>LaG16-F(EcoRI-Kozark)</td><td>GCTGAATTCGCCACCATGGCCCTGCCCGTG</td></tr>
 
<tr><td>O14</td><td>LaG16-F(EcoRI-Kozark)</td><td>GCTGAATTCGCCACCATGGCCCTGCCCGTG</td></tr>
−
<tr><td>O15</td><td>LaG16-B(OE</td><td>mNotch)   </td><td>CTGTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGG</td></tr>
+
<tr><td>O15</td><td>LaG16-B(OE mNotch)   </td><td>CTGTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGG</td></tr>
−
<tr><td>O16</td><td>LaG16-B(OE</td><td>mNCe)     </td><td>GGTTGCTACCCACACAGGGGCTCACGGTCACCTGGG</td></tr>
+
<tr><td>O16</td><td>LaG16-B(OE mNCe)     </td><td>GGTTGCTACCCACACAGGGGCTCACGGTCACCTGGG</td></tr>
−
<tr><td>O17</td><td>mcherry-F(OE</td><td>mNotch) </td><td>CAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr>
+
<tr><td>O17</td><td>mcherry-F(OE mNotch) </td><td>CAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr>
 
<tr><td>O18</td><td>mCherry-B(UAA-HindIII)</td><td>GCTAAGCTTTTACTTGTACAGCTCGTCCATGC</td></tr>
 
<tr><td>O18</td><td>mCherry-B(UAA-HindIII)</td><td>GCTAAGCTTTTACTTGTACAGCTCGTCCATGC</td></tr>
 
<tr><td>O19</td><td>CMV-F(ala-EcoRI-ala-XbaI)    </td><td>GCAGAATTCGCATCTAGAGACATTGATTATTGACGAGTTATTAATAGTAATCAATTACG</td></tr>
 
<tr><td>O19</td><td>CMV-F(ala-EcoRI-ala-XbaI)    </td><td>GCAGAATTCGCATCTAGAGACATTGATTATTGACGAGTTATTAATAGTAATCAATTACG</td></tr>
−
<tr><td>O20</td><td>LacOi-F(OE</td><td>intron)           </td><td>CGTAATTGTGAGCGGATAACAATTACGTTCGCCCCGTGCCCCG               </td></tr>
+
<tr><td>O20</td><td>LacOi-F(OE intron)</td><td>CGTAATTGTGAGCGGATAACAATTACGTTCGCCCCGTGCCCCG               </td></tr>
−
<tr><td>O21</td><td>LacOi-B(OE</td><td>intron)           </td><td>CGTAATTGTTATCCGCTCACAATTACGGGCAACGCAGCGACTC               </td></tr>
+
<tr><td>O21</td><td>LacOi-B(OE intron)</td><td>CGTAATTGTTATCCGCTCACAATTACGGGCAACGCAGCGACTC               </td></tr>
 
<tr><td>O22</td><td>intron-B(SpeI-ala-HindIII-ala)</td><td>GCAAAGCTTGCAACTAGTCTGTAGGAAAAAGAAGAAGGCATGAAC             </td></tr>
 
<tr><td>O22</td><td>intron-B(SpeI-ala-HindIII-ala)</td><td>GCAAAGCTTGCAACTAGTCTGTAGGAAAAAGAAGAAGGCATGAAC             </td></tr>
 
<tr><td>O23</td><td>LaG16-F(G_pML2-EcoRI-Kozark)</td><td>AGTTATTAGGTCCCTCGACGAATTCGCCACCATGGCCCTGCCCGTGACC</td></tr>
 
<tr><td>O23</td><td>LaG16-F(G_pML2-EcoRI-Kozark)</td><td>AGTTATTAGGTCCCTCGACGAATTCGCCACCATGGCCCTGCCCGTGACC</td></tr>
Line 300: Line 302:
 
<tr><td>O25</td><td>LaG16-R(G_mNCe)</td><td>GGGTTGCTACCCACACAGGGGCTCACGGTCACCTGGGTG</td></tr>
 
<tr><td>O25</td><td>LaG16-R(G_mNCe)</td><td>GGGTTGCTACCCACACAGGGGCTCACGGTCACCTGGGTG</td></tr>
 
<tr><td>O26</td><td>mcherry-F(G_mNC)</td><td>TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr>
 
<tr><td>O26</td><td>mcherry-F(G_mNC)</td><td>TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr>
−
<tr><td>O27</td><td>mcherry-R(HindIII</td><td>G_pML2)</td><td>AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG</td></tr>
+
<tr><td>O27</td><td>mcherry-R(HindIII G_pML2)</td><td>AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG</td></tr>
−
<tr><td>O28</td><td>rev_41BB_CD3z9</td><td>序列缺失</td></tr>
+
<!-- tr><td>O28</td><td>rev_41BB_CD3z9</td><td>序列缺失</td></tr -->
 
<tr><td>O29</td><td>hNotch-4168</td><td>CCCTGCCTGGGCGGCAAC</td></tr>
 
<tr><td>O29</td><td>hNotch-4168</td><td>CCCTGCCTGGGCGGCAAC</td></tr>
 
<tr><td>O30</td><td>5286-hNotch</td><td>CCGCCGGCGCTTGCGGG</td></tr>
 
<tr><td>O30</td><td>5286-hNotch</td><td>CCGCCGGCGCTTGCGGG</td></tr>
Line 371: Line 373:
 
<tr><td>O99</td><td>1* ZF 54-8 & 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr>
 
<tr><td>O99</td><td>1* ZF 54-8 & 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr>
 
<tr><td>O100</td><td>2* ZFHD1</td><td>GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG</td></tr>
 
<tr><td>O100</td><td>2* ZFHD1</td><td>GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG</td></tr>
−
<tr><td></td><td></td><td>TAGCGGCCGCTGCAG</td></tr>
+
<tr><td>O101</td><td>1*PIR</td><td style="word-break: break-all">GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG</td></tr>
−
<tr><td>O101</td><td>1*PIR</td><td>GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG</td></tr>
+
 
<tr><td>O102</td><td>pCMV-F(S)    </td><td>cgtgtacggtgggaggtctatataag</td></tr>
 
<tr><td>O102</td><td>pCMV-F(S)    </td><td>cgtgtacggtgggaggtctatataag</td></tr>
 
<tr><td>O103</td><td>pML2-R(S)                </td><td>acgagattccagcaggtcg</td></tr>
 
<tr><td>O103</td><td>pML2-R(S)                </td><td>acgagattccagcaggtcg</td></tr>
Line 429: Line 430:
 
<tr><td>O165</td><td>pCAGR</td><td>tcgcccttgctcaccatgatCTGTAGGAAAAAGAAGAAGG</td></tr>
 
<tr><td>O165</td><td>pCAGR</td><td>tcgcccttgctcaccatgatCTGTAGGAAAAAGAAGAAGG</td></tr>
 
<tr><td>O166</td><td>tTa-R(EcoRI-6)</td><td>GCAGCAGAATTCCTACCCACCGTACTCGTCAATTCC</td></tr>
 
<tr><td>O166</td><td>tTa-R(EcoRI-6)</td><td>GCAGCAGAATTCCTACCCACCGTACTCGTCAATTCC</td></tr>
−
<tr><td>O167</td><td>tTa-R(EcoRI-6</td><td>f)</td><td>GCAGCAGAATTCCCCACCGTACTCGTCAATTCC</td></tr>
+
<tr><td>O167</td><td>tTa-R(EcoRI-6 f)</td><td>GCAGCAGAATTCCCCACCGTACTCGTCAATTCC</td></tr>
 
<tr><td>O168</td><td>EGFP-R(S)</td><td>gctgaacttgtggccgtttac</td></tr>
 
<tr><td>O168</td><td>EGFP-R(S)</td><td>gctgaacttgtggccgtttac</td></tr>
 
<tr><td>O169</td><td>mcherry-R(S)</td><td>TGATGATGGCCATGTTATCCTCC</td></tr>
 
<tr><td>O169</td><td>mcherry-R(S)</td><td>TGATGATGGCCATGTTATCCTCC</td></tr>
Line 532: Line 533:
 
<tr><td>O267</td><td>LaG16-F(L_CD8-alpha-SP2)  </td><td>GACCGCCCTGCTGCTGCCCCTGGCCCTGCTGCTGCACGCCGCCAGACCTGAGCAAAAG</td></tr>
 
<tr><td>O267</td><td>LaG16-F(L_CD8-alpha-SP2)  </td><td>GACCGCCCTGCTGCTGCCCCTGGCCCTGCTGCTGCACGCCGCCAGACCTGAGCAAAAG</td></tr>
 
<tr><td>O268</td><td>CD8-alpha-SP-F(6-AgeI-Kozak)</td><td>GCAGCAACCGGTGCCACCATGGCCCTGCCCGTGACCGCCCTGCTGCTGCCCC    </td></tr>
 
<tr><td>O268</td><td>CD8-alpha-SP-F(6-AgeI-Kozak)</td><td>GCAGCAACCGGTGCCACCATGGCCCTGCCCGTGACCGCCCTGCTGCTGCCCC    </td></tr>
−
<tr><td>O269</td><td>VP64-R(EcoRI-6</td><td>f)         </td><td>GCAGCAGAATTCtagcatatccagatcaaagtcatctagggcatcgg          </td></tr>
+
<tr><td>O269</td><td>VP64-R(EcoRI-6 f)</td><td>GCAGCAGAATTCtagcatatccagatcaaagtcatctagggcatcgg          </td></tr>
 
<tr><td>O270</td><td>pUAS-F(6-BP)</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGcatgtgattacgccaagctacggg</td></tr>
 
<tr><td>O270</td><td>pUAS-F(6-BP)</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGcatgtgattacgccaagctacggg</td></tr>
 
<tr><td>O271</td><td>pUAS-R(BS-6)</td><td>GCAGCACTGCAGCGGCCGCTACTAGTActagagtctccgctcggaggacaga</td></tr>
 
<tr><td>O271</td><td>pUAS-R(BS-6)</td><td>GCAGCACTGCAGCGGCCGCTACTAGTActagagtctccgctcggaggacaga</td></tr>
Line 606: Line 607:
 
<tr><td>O341</td><td>prefix-CD47tm(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr>
 
<tr><td>O341</td><td>prefix-CD47tm(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr>
 
<tr><td>O342</td><td>CD47tm(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC</td></tr>
 
<tr><td>O342</td><td>CD47tm(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC</td></tr>
−
<tr><td>O343</td><td>prefix-hYP9</td><td>1scFv(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr>
+
<tr><td>O343</td><td>prefix-hYP9 1scFv(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr>
−
<tr><td>O344</td><td>hYP9</td><td>1scFv(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC</td></tr>
+
<tr><td>O344</td><td>hYP9 1scFv(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC</td></tr>
 
<tr><td>O345</td><td>prefix-LaG 16-2(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr>
 
<tr><td>O345</td><td>prefix-LaG 16-2(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr>
 
<tr><td>O346</td><td>LaG 16-2(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC</td></tr>
 
<tr><td>O346</td><td>LaG 16-2(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC</td></tr>
Line 648: Line 649:
 
<tr><td>O399</td><td>BS-R          </td><td>TCTGCAGCGGCCGC</td></tr>
 
<tr><td>O399</td><td>BS-R          </td><td>TCTGCAGCGGCCGC</td></tr>
 
<tr><td>O400</td><td>pSB1A3-C(3-BP-BamHI)  </td><td>GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg</td></tr>
 
<tr><td>O400</td><td>pSB1A3-C(3-BP-BamHI)  </td><td>GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg</td></tr>
−
<tr><td>O401</td><td>pSB1A3-C(3-BS-HindIII</td><td>C)</td><td>GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg</td></tr>
+
<tr><td>O401</td><td>pSB1A3-C(3-BS-HindIII C)</td><td>GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg</td></tr>
 
<tr><td>O404</td><td>mNCe-F(M)    </td><td>CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG</td></tr>
 
<tr><td>O404</td><td>mNCe-F(M)    </td><td>CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG</td></tr>
 
<tr><td>O405</td><td>mNCe-F        </td><td>CCTTGTGTTGGtAGTAATCCATGCTAC</td></tr>
 
<tr><td>O405</td><td>mNCe-F        </td><td>CCTTGTGTTGGtAGTAATCCATGCTAC</td></tr>
Line 661: Line 662:
 
<tr><td>O414</td><td>MCS-R(A)    </td><td>GAAGCTTGTCGACTATGGCAGTCTTCGGGCCCGAAGACTGCTCCTGGATCCGAATTCA</td></tr>
 
<tr><td>O414</td><td>MCS-R(A)    </td><td>GAAGCTTGTCGACTATGGCAGTCTTCGGGCCCGAAGACTGCTCCTGGATCCGAATTCA</td></tr>
 
<tr><td>O415</td><td>pCMV-F2(S)  </td><td>cccattgacgcaaatgggc</td></tr>
 
<tr><td>O415</td><td>pCMV-F2(S)  </td><td>cccattgacgcaaatgggc</td></tr>
−
<tr><td>O416</td><td>CD8aSP-F(Kozak</td><td>G_pML2-CX)</td><td>gtgaaTAgtcagatcATcttgagTtACCGGTGAATTCGCCACCATGGCGCTGCCGG</td></tr>
+
<tr><td>O416</td><td>CD8aSP-F(Kozak G_pML2-CX)</td><td>gtgaaTAgtcagatcATcttgagTtACCGGTGAATTCGCCACCATGGCGCTGCCGG</td></tr>
 
<tr><td>O417</td><td>LaG2-R(G_mNCe)          </td><td>ATTaTAGCAaGGATTACTACCAACACAAGGAACAGTGACTTGCGTTCCCTGC</td></tr>
 
<tr><td>O417</td><td>LaG2-R(G_mNCe)          </td><td>ATTaTAGCAaGGATTACTACCAACACAAGGAACAGTGACTTGCGTTCCCTGC</td></tr>
 
<tr><td>O418</td><td>mNCe-F(M)              </td><td>CCTTGTGTTGGTAGTAATCCtTGCTAtAATCAAGGGACCTGCGAGC</td></tr>
 
<tr><td>O418</td><td>mNCe-F(M)              </td><td>CCTTGTGTTGGTAGTAATCCtTGCTAtAATCAAGGGACCTGCGAGC</td></tr>
Line 668: Line 669:
 
<tr><td>O421</td><td>tTAA-F(M)              </td><td>GGaGTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGG</td></tr>
 
<tr><td>O421</td><td>tTAA-F(M)              </td><td>GGaGTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGG</td></tr>
 
<tr><td>O422</td><td>tTAA-R(G_pML2-CX)      </td><td>GAGATATTAGAAGGTATCGAGTTCCCAATTGAAGCTTTCATCCTGGGAGCATATCCAGG</td></tr>
 
<tr><td>O422</td><td>tTAA-R(G_pML2-CX)      </td><td>GAGATATTAGAAGGTATCGAGTTCCCAATTGAAGCTTTCATCCTGGGAGCATATCCAGG</td></tr>
−
<tr><td>O423</td><td>pML2-R(Lin</td><td> L1)</td><td>tcaagATgatctgacTAttcactacagctctgcttatatagacctcccaccgtacacgc</td></tr>
+
<tr><td>O423</td><td>pML2-R(Lin L1)</td><td>tcaagATgatctgacTAttcactacagctctgcttatatagacctcccaccgtacacgc</td></tr>
−
<tr><td>O424</td><td>pML2-R(Lin</td><td> L2)</td><td>CGAATTCACCGGTaActcaagATgatctgacTAttcactacagctc</td></tr>
+
<tr><td>O424</td><td>pML2-R(Lin L2)</td><td>CGAATTCACCGGTaActcaagATgatctgacTAttcactacagctc</td></tr>
−
<tr><td>O425</td><td>pML2-F(Lin</td><td> L2)</td><td>CAAGCTTCAATTGGGAACTCGATACCTTCTAATATCTC</td></tr>
+
<tr><td>O425</td><td>pML2-F(Lin L2)</td><td>CAAGCTTCAATTGGGAACTCGATACCTTCTAATATCTC</td></tr>
 
<tr><td>O426</td><td>pML2-CX(EcoRI) </td><td>gtagtgaaTAgtcagatcATcttgagTtACCGGTG</td></tr>
 
<tr><td>O426</td><td>pML2-CX(EcoRI) </td><td>gtagtgaaTAgtcagatcATcttgagTtACCGGTG</td></tr>
 
<tr><td>O427</td><td>pML2-CX(HindIII)</td><td>TATGAGATATTAGAAGGTATCGAGTTCCCAATTGAAGC</td></tr>
 
<tr><td>O427</td><td>pML2-CX(HindIII)</td><td>TATGAGATATTAGAAGGTATCGAGTTCCCAATTGAAGC</td></tr>
Line 757: Line 758:
 
<tr><td>O510</td><td>mCherry-R(KOD)</td><td>CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCTTGCAGGGAGGAGTCCTGGGTCAC</td></tr>
 
<tr><td>O510</td><td>mCherry-R(KOD)</td><td>CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCTTGCAGGGAGGAGTCCTGGGTCAC</td></tr>
 
<tr><td>O511</td><td>mCherry-F(KOD)</td><td>CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACCATGGGCTGGGAGGCC</td></tr>
 
<tr><td>O511</td><td>mCherry-F(KOD)</td><td>CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACCATGGGCTGGGAGGCC</td></tr>
−
<tr><td>O512</td><td>BP-F(M</td><td> CE_p2Y-EcoRI)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAG</td></tr>
+
<tr><td>O512</td><td>BP-F(M CE_p2Y-EcoRI)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAG</td></tr>
−
<tr><td>O513</td><td>BS(M</td><td> CE_p2Y-AgeI)  </td><td>CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT</td></tr>
+
<tr><td>O513</td><td>BS(M CE_p2Y-AgeI)  </td><td>CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT</td></tr>
−
<tr><td>O514</td><td>BS-R(M</td><td> CE_p2Y-AS)  </td><td>CCCTAACGTGATCTGACAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT</td></tr>
+
<tr><td>O514</td><td>BS-R(M CE_p2Y-AS)  </td><td>CCCTAACGTGATCTGACAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT</td></tr>
 
<tr><td>O515</td><td>PIP-F(CE_p2Y-AS-Kozak)</td><td>ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGTCAAGGGGGGAGGTTAG</td></tr>
 
<tr><td>O515</td><td>PIP-F(CE_p2Y-AS-Kozak)</td><td>ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGTCAAGGGGGGAGGTTAG</td></tr>
 
<tr><td>O516</td><td>KRAB-R(CE_HindIII)  </td><td>GTACTTGAATGAGGGAAGCTTTTACACCAGCCAGGGTTCC</td></tr>
 
<tr><td>O516</td><td>KRAB-R(CE_HindIII)  </td><td>GTACTTGAATGAGGGAAGCTTTTACACCAGCCAGGGTTCC</td></tr>
Line 769: Line 770:
 
<tr><td>O522</td><td>TRE3GV-F(1-BP-M_p2Y)    </td><td>CGAATTCcggccgcttcTAGAGGAGTTTACTCCCTATCagtgatagagaacgtatgaag</td></tr>
 
<tr><td>O522</td><td>TRE3GV-F(1-BP-M_p2Y)    </td><td>CGAATTCcggccgcttcTAGAGGAGTTTACTCCCTATCagtgatagagaacgtatgaag</td></tr>
 
<tr><td>O523</td><td>TRE3GV-R(BS-M_p2Y-AgeI-1)</td><td>GACCGGTCTGCAGacggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc</td></tr>
 
<tr><td>O523</td><td>TRE3GV-R(BS-M_p2Y-AgeI-1)</td><td>GACCGGTCTGCAGacggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc</td></tr>
−
<tr><td>O524</td><td>ZF43-8-F(CE_p2Y</td><td> M)    </td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaGAGTGAGG</td></tr>
+
<tr><td>O524</td><td>ZF43-8-F(CE_p2Y M)    </td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaGAGTGAGG</td></tr>
 
<tr><td>O525</td><td>LaG16-F(CE-P2Y_SalI)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGGTCaccgccttgctcctgc</td></tr>
 
<tr><td>O525</td><td>LaG16-F(CE-P2Y_SalI)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGGTCaccgccttgctcctgc</td></tr>
 
<tr><td>O526</td><td>ZF43-8-F(CE_P2A)        </td><td>TGGAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC</td></tr>
 
<tr><td>O526</td><td>ZF43-8-F(CE_P2A)        </td><td>TGGAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC</td></tr>
Line 817: Line 818:
 
<tr><td>O570</td><td>d2EGFP-R(L3)  </td><td>CACATTGATCCTAGCAGAAGCACAGGCTGCTGGGTGACGGTCCATCCCGCTCTCCTGG</td></tr>
 
<tr><td>O570</td><td>d2EGFP-R(L3)  </td><td>CACATTGATCCTAGCAGAAGCACAGGCTGCTGGGTGACGGTCCATCCCGCTCTCCTGG</td></tr>
 
<tr><td>O571</td><td>d2EGFP-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTCTACACATTGATCCTAGCAGAAGC            </td></tr>
 
<tr><td>O571</td><td>d2EGFP-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTCTACACATTGATCCTAGCAGAAGC            </td></tr>
−
<tr><td>O572</td><td>CD8aSP-F(CE_p2Y</td><td> G3)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG</td></tr>
+
<tr><td>O572</td><td>CD8aSP-F(CE_p2Y G3)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG</td></tr>
 
<tr><td>O573</td><td>EGFP-R(CE_mN1ce)  </td><td>GGATTACTACCAACACAAGGCTTGTACAGCTCGTCCATGCC    </td></tr>
 
<tr><td>O573</td><td>EGFP-R(CE_mN1ce)  </td><td>GGATTACTACCAACACAAGGCTTGTACAGCTCGTCCATGCC    </td></tr>
 
<tr><td>O574</td><td>mN1c-R(C)            </td><td>CCTTCTTCTTTTTCGTGACAGCAAAACTCCGC                      </td></tr>
 
<tr><td>O574</td><td>mN1c-R(C)            </td><td>CCTTCTTCTTTTTCGTGACAGCAAAACTCCGC                      </td></tr>
Line 833: Line 834:
 
<tr><td>O586</td><td>rtTAA-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCatgtctagactggacaagagcaaag</td></tr>
 
<tr><td>O586</td><td>rtTAA-F(CE_p2Y)</td><td>ATCACGTTAGGGCTAGTCGACGCCACCatgtctagactggacaagagcaaag</td></tr>
 
<tr><td>O587</td><td>rtTAA-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTttacccggggagcatgtcaag        </td></tr>
 
<tr><td>O587</td><td>rtTAA-R(CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTttacccggggagcatgtcaag        </td></tr>
−
<tr><td>O588</td><td>VP64-R(MfeI</td><td>CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCACAATTGtagcatatcCagatcaaagtcatctaggg</td></tr>
+
<tr><td>O588</td><td>VP64-R(MfeI CE_p2Y)</td><td>GTACTTGAATGAGGGAAGCTTTCACAATTGtagcatatcCagatcaaagtcatctaggg</td></tr>
 
<tr><td>O589</td><td>ZF21-16-F(CE_P2A) </td><td>GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC</td></tr>
 
<tr><td>O589</td><td>ZF21-16-F(CE_P2A) </td><td>GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC</td></tr>
−
<tr><td>O590</td><td>ZF42-10-F(M</td><td>CE_p2Y)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaGACGCTG</td></tr>
+
<tr><td>O590</td><td>ZF42-10-F(M CE_p2Y)</td><td>accggaacccttaatGAATTCcggccgcttcTAGAGaGACGCTG</td></tr>
 
<tr><td>O591</td><td>CMV-F(BP_M)</td><td>tGAATTCcggccgcttcTAGAGcgttacataacttacggtaaatg    </td></tr>
 
<tr><td>O591</td><td>CMV-F(BP_M)</td><td>tGAATTCcggccgcttcTAGAGcgttacataacttacggtaaatg    </td></tr>
 
<tr><td>O592</td><td>CMV-R(BS_M)</td><td>cACCGGTCTGCAGacggccgcTACTAGTAagctctgcttatatagacctc</td></tr>
 
<tr><td>O592</td><td>CMV-R(BS_M)</td><td>cACCGGTCTGCAGacggccgcTACTAGTAagctctgcttatatagacctc</td></tr>
Line 949: Line 950:
 
<tr><td>O726</td><td>tTAA-R(C)              </td><td>TCCTGGGAGCATATCCAGGTCAAGATCGTC                            </td></tr>
 
<tr><td>O726</td><td>tTAA-R(C)              </td><td>TCCTGGGAGCATATCCAGGTCAAGATCGTC                            </td></tr>
 
<tr><td>O727</td><td>CD8ASP-F(L1_P2A)      </td><td>CTCCCTGCTGAAGCAAGCAGGAGACGTGGAAGAAAACCCGGGACCTATGGCGCTGCCGG</td></tr>
 
<tr><td>O727</td><td>CD8ASP-F(L1_P2A)      </td><td>CTCCCTGCTGAAGCAAGCAGGAGACGTGGAAGAAAACCCGGGACCTATGGCGCTGCCGG</td></tr>
−
<tr><td>O728</td><td>CD8ASP-F(L2_P2A</td><td>CE_tTAA)</td><td>CTGGATATGCTCCCAGGAggctccggcGCCACCAACTTCTCCCTGCTGAAGCAAGC  </td></tr>
+
<tr><td>O728</td><td>CD8ASP-F(L2_P2A CE_tTAA)</td><td>CTGGATATGCTCCCAGGAggctccggcGCCACCAACTTCTCCCTGCTGAAGCAAGC  </td></tr>
 
<tr><td>O729</td><td>LaG2-F-N(S)            </td><td>GTGCTGCCTCCGGTC                                          </td></tr>
 
<tr><td>O729</td><td>LaG2-F-N(S)            </td><td>GTGCTGCCTCCGGTC                                          </td></tr>
 
<tr><td>O730</td><td>tTAA-F-C(S)            </td><td>GCTTAAGTGCGAGAGTGGC                                      </td></tr>
 
<tr><td>O730</td><td>tTAA-F-C(S)            </td><td>GCTTAAGTGCGAGAGTGGC                                      </td></tr>
Line 1,276: Line 1,277:
 
             <!--Abstract on content page-->
 
             <!--Abstract on content page-->
 
             <div id="abstractContent" class="z-depth-2">
 
             <div id="abstractContent" class="z-depth-2">
−
                 <a href="#!"><img src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a>
+
                 <a href="#!"><img alt="2018 team Fudan abstract" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a>
 
                 <div class="container">
 
                 <div class="container">
 
                     <h2 style="margin: 0;padding: 10px 0;">Abstract</h2>
 
                     <h2 style="margin: 0;padding: 10px 0;">Abstract</h2>
Line 1,313: Line 1,314:
 
                     <div class="row">
 
                     <div class="row">
 
                         <div id="sponsor" class="col m3 s12 row">
 
                         <div id="sponsor" class="col m3 s12 row">
−
                             <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png">
+
                             <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png">
 
                             </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png">
 
                             </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png">
 
                         </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png">
 
                         </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png">
Line 1,325: Line 1,326:
 
                                     <span>Project</span>
 
                                     <span>Project</span>
 
                                     <ul>
 
                                     <ul>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Demonstrate">Demonstration</a></li>
+
                                         <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li>
  
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li>
+
                                         <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Results">Transmembrane logic</a></li>
+
                                         <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li>
 
                                         <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li>
 
                                         <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li>
 
                                     </ul>
 
                                     </ul>
Line 1,335: Line 1,336:
 
                                     <span>Dry lab</span>
 
                                     <span>Dry lab</span>
 
                                     <ul>
 
                                     <ul>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Addon#ribo">Addon: ribo</a></li>
+
                                         <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Addon#TALE">Addon: TALE</a></li>
+
                                         <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Addon#T2">Addon: T2</a></li>
+
                                         <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
+
                                         <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Model#War_Predictor">Model: war&nbsp;predictor</a></li>
+
                                         <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand&nbsp;kinetics</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Software">Software</a></li>
+
                                         <li><a href="/Team:Fudan/Software">Software</a></li>
 
                                     </ul>
 
                                     </ul>
 
                                 </div>
 
                                 </div>
Line 1,346: Line 1,347:
 
                                     <span>Wet lab</span>
 
                                     <span>Wet lab</span>
 
                                     <ul>
 
                                     <ul>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/InterLab">iGEM interLab</a></li>
+
                                         <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Notebook">Our notebook</a></li>
+
                                         <li><a href="/Team:Fudan/Notebook">Our notebook</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Primers">Primers used</a></li>
+
                                         <li><a href="/Team:Fudan/Primers">Primers used</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Protocols">Protocols</a></li>
+
                                         <li><a href="/Team:Fudan/Protocols">Protocols</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Safety">Safety</a></li>
+
                                         <li><a href="/Team:Fudan/Safety">Safety</a></li>
 
                                     </ul>
 
                                     </ul>
 
                                 </div>
 
                                 </div>
Line 1,358: Line 1,359:
 
                                     <span>Toolbox</span>
 
                                     <span>Toolbox</span>
 
                                     <ul>
 
                                     <ul>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Basic_Parts">Basic parts</a></li>
+
                                         <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Composite_Parts">Composite parts</a></li>
+
                                         <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Optimization">Optimization</a></li>
+
                                         <li><a href="/Team:Fudan/Optimization">Optimization</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Parts_Collection">Parts collection</a></li>
+
                                         <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Improve">Parts improvement</a></li>
+
                                         <li><a href="/Team:Fudan/Improve">Parts improvement</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Measurement">Quantification</a></li>
+
                                         <li><a href="/Team:Fudan/Measurement">Quantification</a></li>
 
                                     </ul>
 
                                     </ul>
 
                                 </div>
 
                                 </div>
Line 1,369: Line 1,370:
 
                                     <span>Outreach</span>
 
                                     <span>Outreach</span>
 
                                     <ul>
 
                                     <ul>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Bio-Art">Bio-Art display</a></li>
+
                                         <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Collaborations">Collaborations</a></li>
+
                                         <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Design_Intention">Design intention</a></li>
+
                                         <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Human_Practices">Human practices</a></li>
+
                                         <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li>
−
                                         <li><a href="https://2018.igem.org/Team:Fudan/Public_Engagement">Public engagement</a></li>
+
                                         <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li>
 
                                     </ul>
 
                                     </ul>
 
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Latest revision as of 15:20, 7 November 2018

2018 iGEM Team:Fudan - Primers

Primers used

Here we present a library of the vast collection of primers we have designed

Primers used

Here we present a library of the vast collection of primers we have designed

Primers

#Oligo NameDNA Sequence (5 to 3)
O1pLL7.1-F(S)GGTCTATATAAGCAGAGCTGG
O2pLL7.1-R(S)AACCGGAACCCTTAAACATG
O3LaG16-F(G_pLL7.1)TGAACCGTCAGATCCGCTAGAATTCGCCACCATGGCCCTGCCCGTGACC
O4LaG16-R(G_mNc)AGTCCAGGATGCTCACGGTCACCTGGGTG
O5mNc-F(G_LaG16)GACCGTGAGCATCCTGGACTACAGCTTCACAGGTGGCGC
O6mNc|e-R(G_tTa)ATCTAGACATCCGCCGGCGCTTGCGGGA
O7tTa-F(G_mNc|e)GCGCCGGCGGATGTCTAGATTAGATAAAAGTAAAGTGATTAAC
O8tTa-R(G_pLL7.1)CCCTCGACGAATTGCCTGCAGGCTACCCACCGTACTCGTCAATTC
O9LaG16-R(G-mNc(e))CCACACAGGGGCTCACGGTCACCTGGGTG
O10mNc(e)-F(G_LaG16)GACCGTGAGCCCCTGTGTGGGTAGCAACCCCTGCTAC
O11mNotch 1 core-PFATCCTGGACTACAGCTTCACAG
O12mNotch 1 core (extended)-PFCCCTGTGTGGGTAGCAACC
O13mNotch 1 core-PBCCGCCGGCGCTTG
O14LaG16-F(EcoRI-Kozark)GCTGAATTCGCCACCATGGCCCTGCCCGTG
O15LaG16-B(OE mNotch)   CTGTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGG
O16LaG16-B(OE mNCe)     GGTTGCTACCCACACAGGGGCTCACGGTCACCTGGG
O17mcherry-F(OE mNotch) CAAGCGCCGGCGGATGGTGAGCAAGGGCGAG
O18mCherry-B(UAA-HindIII)GCTAAGCTTTTACTTGTACAGCTCGTCCATGC
O19CMV-F(ala-EcoRI-ala-XbaI)    GCAGAATTCGCATCTAGAGACATTGATTATTGACGAGTTATTAATAGTAATCAATTACG
O20LacOi-F(OE intron)CGTAATTGTGAGCGGATAACAATTACGTTCGCCCCGTGCCCCG               
O21LacOi-B(OE intron)CGTAATTGTTATCCGCTCACAATTACGGGCAACGCAGCGACTC               
O22intron-B(SpeI-ala-HindIII-ala)GCAAAGCTTGCAACTAGTCTGTAGGAAAAAGAAGAAGGCATGAAC             
O23LaG16-F(G_pML2-EcoRI-Kozark)AGTTATTAGGTCCCTCGACGAATTCGCCACCATGGCCCTGCCCGTGACC
O24LaG16-R(G_mNC)GTGAAGCTGTAGTCCAGGATGCTCACGGTCACCTGGGTG
O25LaG16-R(G_mNCe)GGGTTGCTACCCACACAGGGGCTCACGGTCACCTGGGTG
O26mcherry-F(G_mNC)TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG
O27mcherry-R(HindIII G_pML2)AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG
O29hNotch-4168CCCTGCCTGGGCGGCAAC
O305286-hNotchCCGCCGGCGCTTGCGGG
O31tTA-startCAAGCGCCGGCGG ATGTCTAGATTAGATAAAAGTAAAGTG 
O32rev_tTA-end-3NotI-2-Hind3-5GTTCCAAGCTTAAGCGCTACCCACCGTACTCG
O33EGFP_BglⅡ_FGCAGATCTatggtgagcaagggcga
O34EGFP_XbalI_RGCTCTAGActttacttgtacagctcgtccat
O35rtTA_pLL7.0_FTGAACCGTCAGATCCGCTAGAAGTTTGTACAAAAAAGCAG
O36rtTA_EGFP_RTGGCACTTTTTTTAAATGATTTGCCCTCCC
O37rtTa_EGFP_FGCAAATCATTTAAAAAAAGTGCCACCTGACGTC
O38LacI_pLL7.0_R GCAAATCATTTAAAAAAAGTGCCACCTGACGTC
O39lacI_mFF4_ployA_FTACCGCGGGCCCGGGATCCATTTGACAATTAAAGCTTGAGG
O40mFF4_ployA_pCAGop_RattaataactaGCGCAGCGAGTCAGTGAG
O41mFF4_polyA_pCAGop_FctcgctgcgcTAGTTATTAATAGTAATCAATTACGGG
O42pCAGop_pLL7.0_RCTTGCTCACCATGGTGGCGACTGTAGGAAAAAGAAGAAGG
O43mFF4_pll7.0_RCTTGCTCACCATGGTGGCGAGCGCAGCGAGTCAGTGAG
O44TRE_pLL7.0_RcgcggtaccgtcgactgcagTACCGAGCTCGAAATCTC
O45TRE_GAL4_FTACCGCGGGCCCGGGATCCAAAGTTTGTACAAAAAAGCAG
O46GAL4_UAS5_RTCACATGAGATACAGTCAACTGTCTTTG
O47GAS4_UAS5_FgttgactgtaTCTCATGTGATTACGCCAAG
O48UAS5_mCherry_RcttgctcaccatggtggcgaTGCTTTTTTGTACAAACTTGG
O49pLL7.0_mCherry_Faagcttatcgataatcaacctctggattacaaaatttgtgaaaga
O50pLL7.0_mCherry_RGATATCGAATTGTACCTAGTGGAACCGGAACC
O51mCherry_FF4_polyA_FactaggtacaattcgatatcGAATTCTGCAGTCGACGG
O52FF4_ployA_pLL7.0_RggttgattatcgataagcttTTTGCCCTCCCATATGTC
O53EGFPpoly(A)FagtttggttagtaccgcgctGCAAATCATTTAAAAAAAGTGCCACC
O54EGFPpoly(A)RcctggagaatgccaccATGGTGAGCAAGGGCGAG
O55Bi_TRE FtgctcaccatggtggcATTCTCCAGGCGATCTGACGG
O56Bi_TRE RcgcggtaccgtcgactgcaggaattcCGCGGTACCGTCGACTGC
O57pll7.0.1 Fcagggatattcaccattatcgtttcagacc
O58poly(A)FcgacggtaccgcgggcccggAACTTGTTTATTGCAGCTTATAATG
O59poly(A)RcaccatggtggcgaccggtgGCAGTGAAAAAAATGCTTTATTTG
O60BiTRE F ccatggtggcattctccaggGCCTCAGGAGATCTcAATTCtcc
O61BiTRE R cgcggtaccgtcgactgcagaattctaccgagctcgaaatctcca
O62 pSB1C3-VR ATTACCGCCTTTGAGTGAGC
O63 pSB1C3-VF TGCCACCTGACGTCTAAGAA
O64pSB1C3-BP  GAATTCGCGGCCGCTTC
O65pSB1C3-BS CTGCAGCGGCCGCTAC
O66EGFP-F(G_pQM6.2)   gcaagagcaagggccaattgGTGAGCAAGGGCGAGGAG
O67EGFP-R(G_P2A)      gtcgacccCTTGTACAGCTCGTCCATGC
O68P2A-F(G_EGFP)      ctgtacaagGGGTCGACGGGCTCCGGC
O69mcherry-R(G_pQM6.2)ccctttttctggagacaagcttTTACTTGTACAGCTCGTCCATGCCG
O70pQM6.2-R(L-P2A) ggatctggggccggggttctcc
O71pQM6.2-F(L-EGFP)atggtgagcaagggcgaggagc
O72alpha-SP-LaG16-F(3-AgeI)GCAACCGGTGCCACCATGGCCCTGC
O73tTa-R(HindIII-3)            GCAAAGCTTCTACCCACCGTACTCGTCAATTCc
O74P-minCMV-FGAATTCGCGGCCGCTTCTAGgaggtaggcgtgtacggtg
O75S-minCMV-RCTGCAGCGGCCGCTACTAGTAggcgatctgacggttcactaaac
O76P-pSV40-FGAATTCGCGGCCGCTTCTAGgtgtgtcagttagggtgtgg
O77S-pSV40-RCTGCAGCGGCCGCTACTAGTAtttgcaaaagcctaggcctccaaaaaag
O82SV40NLS-KRAB-FCCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg
O83BS-Ftactagtagcggccgctgc
O84BP-Rctctagaagcggccgcg
O85KRAB-R caccagccagggttcctc
O86mcherry-F ATGGTGAGCAAGGGCGAGGAGGATAACATG
O87pTight-F(S) AGCTCGTTTAGTGAACCGTCAG
O883 LTR (Delta-U3)-R(S)GCGTTACTTAAGCTAGCTTGCC
O89P2A-F(S) ACCAACTTCTCCCTGCTGAAG
O90mcherry-R(S) CATGTGCACCTTGAAGCGC
O91BP protecttttctggaattcgcggccg
O92BS protectgccggactgcagcg
O932* ZF 42-10 operatorGAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAC GCT GCT c TACTAGTAGCGGCCGCTGCAG
O942* ZF 54-8 operatorGAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG
O952* ZF 43-8 operatorGAATTCGCGGCCGCTTCTAG a GAG TGA GGA c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG
O962* ZF 21-16 operatorGAATTCGCGGCCGCTTCTAG a TTA GAA GTG a a TTA GAA GTG a TACTAGTAGCGGCCGCTGCAG
O971* ZF 42-10 & 54-8 operatorGAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a TGG GTG GCA t TACTAGTAGCGGCCGCTGCAG
O981* ZF 42-10 & 43-8 operatorGAATTCGCGGCCGCTTCTAG a GAC GCT GCT c a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG
O991* ZF 54-8 & 43-8 operatorGAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG
O1002* ZFHD1GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG
O1011*PIRGAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG
O102pCMV-F(S) cgtgtacggtgggaggtctatataag
O103pML2-R(S) acgagattccagcaggtcg
O106pSB1C3-BP(L)tttctggaattcgcggccgcttc
O107pSB1C3-BS(L)gccggactgcagcggccgc
O108PIR-F(6-XbaI)GCCGCTTCTAGagGAAATAGCGCTGTACAGCG
O1092*ZFHD1-F(6-XbaI)GCCGCTTCTAGagTAATGATGGGCGTATAATGATGG
O110CD8-alpha-SP-F(G_pML2)gatccgctagcgctaccggtgccaccATGGCCTTACCAGTGACCGCCTTG
O111alpha-GPC3-R(G_mNce)acacagggTGGTCGCGGCGCTGGCGT
O112mNce-F(G_alpha-GPC3)cgcgaccaCCCTGTGTGGGTAGCAACC
O113tTa-R(G_pML2) gttatattaagggttccaagcttCTACCCACCGTACTCGTCAATTC
O114alpha-GPC3-R(G_mNc) TCCAGGATTGGTCGCGGCGCTGGCGT
O115mNc-F(G_alpha-GPC3) cgcgaccaATCCTGGACTACAGCTTCACAG
O116CD8-alpha-F2(G_pML2)GATCCGCTAGCGCTACCGGTGCCACCATGGCCCTGCCCGTGACC
O117CD8-alpha-SP-F(G_pML2)cgtcagatccgctagcgctaccggtGCCACCATGGCCCTGCCCG
O118KRAB-R(G_pML2) aagttatattaagggttccaagctttcaCACCAGCCAGGGTTCCTC
O119mNc|e-R(G_Gal4DBD) gcagcttcatCCGCCGGCGCTTGCGGGA
O120Gal4DBD-F(G_mNc|e) gcgccggcggATGAAGCTGCTGAGCAGCATC
O121Gal4DBD-R(G_SV40NLS)tacctcctcctccCACTGTCAGCTGCCGCTG
O122SV40NLS-F(G_Gal4DBD)gctgacagtgGGAGGAGGAGGTAGTCCAAAAAAG
O123mNc|e-R(G_PIP) cccttgacatCCGCCGGCGCTTGCGGGA
O124PIP-F(G_mNc|e) gcgccggcggATGTCAAGGGGGGAGGTTAG
O125PIP-R(G_SV40NLS) tacctcctcctccGGCCTGCTCCACCATAGC
O126SV40NLS-F(G_PIP) ggagcaggccGGAGGAGGAGGTAGTCCAAAAAAG
O127mNc|e-R(G_ZF21-16) tagggctagcCCGCCGGCGCTTGCGGGA
O128ZF21-16-F(G_mNc|e) gcgccggcggGCTAGCCCTAGAGTGAGAACAGG
O129ZF21-16-R(G_SV40NLS)tacctcctcctccGGATCCCTTCTCGCCTGTG
O130SV40NLS-F(G_ZF21-16)gaagggatccGGAGGAGGAGGTAGTCCAAAAAAG
O131mNc|e-R(G_ZF42-10) tagggctagcCCGCCGGCGCTTGCGGGA
O132ZF42-10-F(G_mNc|e) gcgccggcggGCTAGCCCTAGAGTGAGAACAG
O133ZF42-10-R(G_SV40NLS)tacctcctcctccGGATCCCTTTTCTCCGGTG
O134SV40NLS-F(G_ZF42-10)aaagggatccGGAGGAGGAGGTAGTCCAAAAAAG
O135mNc|e-R(G_ZF43-8) tggggctagcCCGCCGGCGCTTGCGGGA
O136ZF43-8-F(G_mNc|e) gcgccggcggGCTAGCCCCAGAGTGAGAAC
O137ZF43-8-R(G_SV40NLS) tacctcctcctccGGATCCTTTTTCACCTGTATGTG
O138SV40NLS-F(G_ZF43-8) aaaaggatccGGAGGAGGAGGTAGTCCAAAAAAG
O139mNc|e-R(G_ZF54-8) tgggagaagcCCGCCGGCGCTTGCGGGA
O140ZF54-8-F(G_mNc|e) gcgccggcggGCTTCTCCCAGAGTAAGAACGGG
O141ZF54-8-R(G_SV40NLS) tacctcctcctccGGACCCTTTTTCGCCGGTG
O142SV40NLS-F(G_ZF54-8) aaaagggtccGGAGGAGGAGGTAGTCCAAAAAAG
O143mNc|e-R(G_ZFHD1) atgggcgttcCCGCCGGCGCTTGCGGGA
O144ZFHD1-F(G_mNc|e) gcgccggcggGAACGCCCATATGCTTGC
O145ZFHD1-R(G_SV40NLS) tacctcctcctccGTTGATTCTTTTTTCTTTCTGGC
O146SV40NLS-F(G_ZFHD1) aagaatcaacGGAGGAGGAGGTAGTCCAAAAAAG
O147EcoRV-mcherry-F(KOD)GATATCATGGTGAGCAAGGGCGAGGAGG
O148P2A-F(6-AgeI-EcoRI)GCAGCAACCGGTGGTGGAGAATTCggAtcAggTgccaccaacttctccctgctgaag
O149EGFP-R(HindIII-6) GCAGCAAAGCTTTTActtgtacagctcgtccatgccga
O150KRAB-R(G_P2A)AGTTGGTGGCACCTGATCCGAATTCCACCAGCCAGGGTTCCTCGC
O151KRAB-F(GGGGS-SV40NLS)GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAatggacgccaagagcctg
O160lacIFaccccggccccagatccgatATGAAACCAGTAACGTTATAC
O161LacIRggtaccgtcgAACCTTCCTCTTCTTCTTAG
O162SV40FgaggaaggttCGACGGTACCGCGGGCCC
O163sv40RattaataactaCACCATGGTGGCGACCGGTG
O164pCAGFcaccatggtgTAGTTATTAATAGTAATCAATTACGGG
O165pCAGRtcgcccttgctcaccatgatCTGTAGGAAAAAGAAGAAGG
O166tTa-R(EcoRI-6)GCAGCAGAATTCCTACCCACCGTACTCGTCAATTCC
O167tTa-R(EcoRI-6 f)GCAGCAGAATTCCCCACCGTACTCGTCAATTCC
O168EGFP-R(S)gctgaacttgtggccgtttac
O169mcherry-R(S)TGATGATGGCCATGTTATCCTCC
O170PIR/ZFD FtttctggaattcgcggccgcttcTAGag
O171pSBIC3 FGgcagaatttcagataaaaaaaatccttagctttcgctaaggatg
O172pSBIC3 Rgcagggtggtgacaccttgcccttttttg
O173NPminCMVGAATTCGCGGCCGCTTCTAgagaggtaggcgtgtacggtg
O174P2A-R(G_EcoRV-mcherry)TCGGATCTGGGGCCGGGGTTCTCCTCCACGTCGC
O175mcherry-F(G_P2A-EcoRV)ccggccccAGATCCGATATCATGGTGAGCAAG
O176SV40poly(A)-FAACTTGTTTATTGCAGCTTATAATGG
O177SV40poly(A)-R1TACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTG
O178SV40poly(A)-R2GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACC
O179rtTA-F-(AgeI)tttctgACCGGTATGTCTAGATTAGATAAAAGTAAAGTGATTAACAGCG
O180P2A-R(G2_mcherry)ccatggtggcgatatcGGGGCCGGGGTTCTCCTC
O181mcherry-F(G2_P2A)ccggccccgatatcgccaccATGGTGAGCAAGGGCGAGGagg
O182Kaba_B_RtaaacaagttctaCACCAGCCAGGGTTCCTC
O183LPA_FgctggtgtagAACTTGTTTATTGCAGCTTATAATG
O184LPA_RcatccttagcGATCCAGACATGATAAGATAC
O185Prom_FtgtctggatcGCTAAGGATGATTTCTGGAATTCGCGGC
O186Prom_RttgctcaccatggtggcgatTTGCCGGACTGCAGCGGC
O187TF1_F(same with tf4)aggagaaccccggccccgatGCTAGCCCTAGAGTGAGAAC
O188TF2_FaggagaaccccggccccgatGCTAGCCCCAGAGTGAGAAC
O189TF3_FaggagaaccccggccccgatGCTTCTCCCAGAGTAAGAACG
O190TF8_FaggagaaccccggccccgatGAACGCCCATATGCTTGC
O191TF9_FaggagaaccccggccccgatATGTCAAGGGGGGAGGTTAG
O192lacI_FaggagaaccccggccccgatATGAAACCAGTAACGTTATAC
O193LacI_RtaaacaagttctaAACCTTCCTCTTCTTCTTAG
O194lacI_40_FgaggaaggtttagAACTTGTTTATTGCAGCTTATAATG
O195LacI_40_RattaataactaGATCCAGACATGATAAGATAC
O196pCAGop_FtgtctggatcTAGTTATTAATAGTAATCAATTACGGG
O197pCAGop_RttgctcaccatggtggcgatCTGTAGGAAAAAGAAGAAGG
O198VP64-F(GGGGS-SV40NLS)GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAGACGCGCTGGACGatt
O199VP64-R TAACATATCGAGATCGAAATCGTCCAGag
O200VP64-F2(GSSSS-SV40NLS)GGAGGAGGAGGTAGTCCAAAAAAGAAGAGAAAGGTAgatgctttagacgattttgactt
O201VP64-R2 tagcatatctagatcaaagtcatctagg
O202rtTAA-F(6-AgeI) GCAGCAACCGGTatgtctagactggacaagagcaaag
O203rtTAA-R(HindIII-6)TGCTGCAAGCTTttacccggggagcatgtcaag
O204gal_FaggagaaccccggccccgatATGAAGCTGCTGAGCAGC
O205Gal_poly(a)_RacatgagatcGATCCAGACATGATAAGATAC
O206Uas_FtgtctggatcGATCTCATGTGATTACGCC
O207Uas_RttgctcaccatggtggcgatTGTACAAACTTGGGTCGAATTG
O208PGK-F(6-EcoRI-6-BamHI-6-MfeI)GCAGCAGAATTCGGTGGAGGATCCGGAGGTCAATTGgggtaggggaggcgc
O209PuroR-R(NotI-6) TGCTGCGCGGCCGCtcaggcaccgggcttg
O210EGFP-R(NotI-6-HindIII6)TGCTGCAAGCTTTGCAGCGCGGCCGCTTActtgtacagctcgtccatgccgag
O211rtTAA-F(6-EcoRI)GCAGCAGAATTCatgtctagactggacaagagcaaag
O212rtTAA-R(BamHI-6)TGCAGCGGATCCttacccggggagcatgtcaag
O213Gal4DBD-FATGAAGCTGCTGAGCAGCatc
O214PIP-FATGTCAAGGGGGGAGGTTAG
O215ZF21-16|24-10-FGCTAGCCCTAGAGTGAGAACAGG
O216ZF43-8-FGCTAGCCCCAGAGTGAGAAC
O217ZF54-8GCTTCTCCCAGAGTAAGAACGG
O218ZFHD1-FGAACGCCCATATGCTTGCC
O219M13fwdLgtgtaaaacgacggccagtg
O220M13revLgctatggacaggaaacagctatgac
O221G_puro_FtgacatgctccccgggtaaaGCAGCAGAATTCGGTGGAGGATC
O222G_puro_RaagttatattaagggttccaTCAGGCACCGGGCTTGCG
O223G_pCAGop_RttgctcaccatggtggcgatATGAGACAGCACAACAAC
O224PGK-R(S)gtgtagcgccaagtgcc
O225Ne-R(KOD)CCCTGATTGTAGCAGGGGTTGCTAC
O226Ne-F(KOD)CACCTGTGAGCCCACATCCGAGAAC
O227M13revL2AATTTCACACAGGAAACAGCTATGAC
O228EGFP-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCatggtgagcaagggcgag
O229mCherry-F(6-Kozak-MfeI)GCAGCAICAATTGGCCACCATGGTGAGCAAGGGCGAG
O230PGK-F(6-EcoRI-6-BamHI-6-SalI)GGAGGTGAATTCGGTGGAGGATCCGGAGGTGTCGACgggtaggggaggcgc
O231PGK-R(MfeI-6)GCAGCACAATTGcgaaaggcccggagatgag
O232mCherry-R(NotI-6-HindIII-6)GGTGGTAAGCTTGGTGGAGCGGCCGCTTACTTGTACAGCTCGTCCATGCC
O233Gal4DBD-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCATGAAGCTGCTGAGCAGCatc
O234PIP-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCATGTCAAGGGGGGAGGTTAG
O235ZF21-16|42-10-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCatgGCAAGCCCTAGAGTGAGAACAGgttc
O236ZF43-8-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCatgGCAAGCCCCAGAGTGAGAACTGGTTCTAAGACACCTCC
O237ZF54-8-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCatgGCTTCTCCCAGAGTAAGAACGG
O238ZFHD1-F(6-MfeI-Kozak)GCAGCACAATTGGCCACCATGGAACGCCCATATGCTTGCc
O239KRAB-R(TAA-HindIII-6)GCAGCAAAGCTTTTACACCAGCCAGGGTTCCTC
O240VP64-R(TAA-HindIII-6)GCAGCAAAGCTTTTAtagcatatctagatcaaagtcatctagggcatc
O241P2A-R(MfeI-6-HindIII-6)TCCTCCAAGCTTTCCTTACAATTGGGGGCCGGGGTTCTC
O242EGFP|mCherry-F(6-AgeI-6-EcoRI-Kozak)GGAGGAACCGGTGGAGGTGAATTCGCCACCATGGTGAGCAAGGGCGAGGAG
O243EGFP|mCherry-F(6-BamHI-AgeI)GCAGCAGGATCCACCGGTgccaccatggtgagcaagggcgag
O244L.Lanier loxp seqtggtgggtacctagtggaacc
O245pSB1C3-BS(L)(AgeI-6)GCAGCAACCGGTgccggactgcagcggccgc
O246EGFP-F(L_CD8-alpha-SP1)tgctgctgcccctggccctgctgctgcacgccgccagaccTatggtgagcaagggcgag
O247EGFP-F(L_CD8-alpha-SP2)ATGgccctgcccgtgaccgccctgctgctgcccctgg
O248CD8-alpha-SP-F(6-AgeI)GCAGCAACCGGTGCCACCATGgccctgcccgt
O249EGFP-R(OE_CD86tm) gtgaagataaaagccgcttgtacagctcgtccatgc
O250CD86tm-F(OE-EGFP) ggacgagctgtacaagcggcttttatcttcacctttctct
O251CD86-R(HindIII-6) GCAGCAAAGCTTTCAatcccgaggccgcttc
O252CD86tm-F(L)cggcttttatcttcacctttctctatagagcttgaggaccctcagc
O253uas-t-Rgagatgatccagacatgataagatacattg
O254uas-t-Fcttctggatccaattcgacccaag
O255Sv40-UAS-FtatcatgtctggatcatctcGTGTGTCAGTTAGGGTGTG
O256SV40-UAS-RtggcgtaatcTTTGCAAAAGCCTAGGCC
O257Gal4BDFcttttgcaaaGATTACGCCAAGCTACGG
O258Gal4BDRggtcgaattggatccagaagTATCTAGAGTCTCCGCTC
O259mCherry-F(6-Kozak-MfeI)GCAGCACAATTGGCCACCATGGTGAGCAAGGGCGAG
O260UAS-prettctggAATTCGCGGCCGCTTCTAGaggatcatctcgtgtgtcagtta
O261UAS-sufgccggacTGCAGCGGCCGCTACTAGTAagaaggtaactagagtctccgctcg
O262LaG16-F(L_CD8-alpha-SP1)CGCCAGACCTGAGCAAAAGTTGATCTCAGAGGAGGACCTCATGACCATGGCCCAGGTG
O263P2A-R(L_CD8-alpha-SP1)GCAGGGCCAGGGGCAGCAGCAGGGCGGTCACGGGCAGGGCCATGGGGCCGGGGTTCTC
O264P2A-R(L_CD8-alpha-SP2)CTCCTCTGAGATCAACTTTTGCTCAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC
O265P2A-R(L_CD8-alpha-SP2-HA) GTAATCTGGCACGTCATAGGGGTAAGGTCTGGCGGCGTGCAGCAGCAGGGCCAGGGGC
O266alpha-GPC3-F(L_CD8-alpha-SP1)CTTACCCCTATGACGTGCCAGATTACGCGGATGTTGTGATGACTCAGTCTCCACTCTC
O267LaG16-F(L_CD8-alpha-SP2) GACCGCCCTGCTGCTGCCCCTGGCCCTGCTGCTGCACGCCGCCAGACCTGAGCAAAAG
O268CD8-alpha-SP-F(6-AgeI-Kozak)GCAGCAACCGGTGCCACCATGGCCCTGCCCGTGACCGCCCTGCTGCTGCCCC
O269VP64-R(EcoRI-6 f)GCAGCAGAATTCtagcatatccagatcaaagtcatctagggcatcgg
O270pUAS-F(6-BP)GCAGCAGAATTCGCGGCCGCTTCTAGAGcatgtgattacgccaagctacggg
O271pUAS-R(BS-6)GCAGCACTGCAGCGGCCGCTACTAGTActagagtctccgctcggaggacaga
O272P2A-F(S_6-BP)GCAGCAGAATTCGCGGCCGCTTCTAGAGACCAACTTCTCCCTGCTGAAG
O273pML2-R(S_BS-6)GCAGCACTGCAGCGGCCGCTACTAGTAacgagattccagcaggtcg
O274pCMV-F(S_6-BP)GCAGCAGAATTCGCGGCCGCTTCTAGAGcgtgtacggtgggaggtctatataag
O275mTagBFP2-F(4-EcoRV-Kozak)CGGccccGATATCGCCAccATGGTGTCTAAGGGCGAAGAGCTGATTAAGGAGAACATGC
O276mTagBFP2-F(L1-T2A) GGGCAGGGGAAGTCTTCTAACtTGCGGGGACGTGGAGGAAAATCCCGGCCCCGATATCG
O277mTagBFP2-F(L2-T2A) GCAGCAaCCGGTGGTGAATTCGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC
O278mTagBFP2-R(HindIII-6) GCAGCAAAGCTTTTAATTAAGtTTGTGCCCCAGTTTGCTAGG
O279EBFP2-F(4-EcoRV-Kozak)CGGCCCCGATATCGCCAccatggtgagcaagggcg
O280EBFP2-R(HindIII-6) GCAGCAAAGCTTttacttgtacagctcgtccatg
O281mTagBFP2-F(L2-T2A)GCAGCAGAATTCGGTaCCGGTGGACAATTGggAtcAggTGAGGGCAGGGGAAGTC
O282PIP-F(6-AgeI)GCAGCAACCGGTGCCACCATGTCAAGGGGGGAGGTTAG
O283ZF43-8-F(6-AgeI)GCAGCAACCGGTGCAAGCCCCAGAGTGAGAAC
O284PIP-F(6-AgeI-Kozak)GCAGCAACCGGTGCCACCatggtgTCAAGGGGGGAGGTTAGAATGG
O285ZF43-8-F(6-AgeI-Kozak)GCAGCAACCGGTGCCACCatgGCAAGCCCCAGAGTG
O286P2A-F(6-SbfI-C)GCAGCACCTGCAGGCggctccggcgccac
O287KRAB-R(MfeI-6)GCAGCACAATTGcaccagccagggttcctc
O288mTagBFP2-NR(S)GCACTTGAAGTGATGGTTGTCC
O289P2A-F(3-AgeI-Kozak-ATG-EcoRI-BamHI-SalI)GCAACCGGTGAATTCGCCACCATGGGATCCGTCGACGGATCAGGTGCCACCAAC
O290mCherry-R(CAAX-HindIII-1) TAAGCTTtcaggagagcacacacttgcagctcatgcagccCTTGTACAGCTCGTCCATG
O291mCherry-F(LifeAct-L1) AGTCCATCTCCAAGGAGGAGGGCGATCCCCCGGTCGCCACCATGGTGAGCAAGGGCGAG
O292mCherry-F(LifeAct-L2) ATGGGCGTGGCCGACTTGATCAAGAAGTTCGAGTCCATCTCCAAGGAGGAGG
O293LifeAct-F(3-EcoRI-BamHI-Kozak)GCAGAATTCGGATCCGCCACCATGGGCGTGGCCGAC
O294mCherry-R(P2A-L1) CGCcggcctgcttcagcagggagaagttggtggcgccggagccCTTGTACAGCTCGTCC
O295mCherry-R(P2A-L2) TAAGCTTGTCGACCTACAATTGGGGGCCGGGGTTCTCctccacgtcgccggcctgcttc
O296Hist2h2bb-F(3-EcoRI-BamHI-Kozak)GGTGAATTCGGATCCGCCACCatgccggacccagcgaag
O297Hist2h2bb-R(M) cgttcacgaaggaGttcatgattcccatagccttagatgagatg
O298Hist2h2bb-F(M) catgaaCtccttcgtgaacgaCatcttcgagcgcatcgc
O299Hist2h2bb-R(OE_mCherry) CATTgtGgcCacAggAggGtccttggagctggtgtacttggtg
O300mCherry-F(OE_Hist2h2bb) acccTccTgtGgcCacAATGGTGAGCAAGGGCGAG
O301mCherry-R(OE_mito)cgcctctcccgttCTTGTACAGCTCGTCCATGCC
O302mito-F(OE_mCherry)GTACAAGaacgggagaggcggtagac
O303mito-R(HindIII-3)GCAAAGCTTttaattattttttcttaattgaataattttgataaacgcccc
O304Myc-FGAGCAGAAATTGATCTCTGAAGAGGAC
O305pML2-C0-Rgcatacattatacgaagttatattaagggttccaag
O306mNce-F CCCTGCGTCGGTAGCAAC
O307tTAA-F(3-MfeI) GCACAATTGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O308tTAA-R(HindIII-3)GCAAAGCTTTCATCCTGGGAGCATATCCAGG
O309TetOn3G-F(3-MfeI) GCACAATTGATGAGCCGACTGGACAAGAG
O310TetOn3G-R(HindIII-R)GCAAAGCTTTTAGCCCGGCAACATATCAAGATC
O311pSV40-F(3-BP) GCAGAATTCGCGGCCGCTTCTAGAGgtgtgtcagttagggtgtgg
O312pSV40-R(BS-AgeI-3)GCAACCGGTctgcagcggccgcTACTAGTAtttgcaaaagcctaggcctcc
O313pTight-F(3-BP) GCAGAATTCGCGGCCGCTTCTAGAGGCCGGAATTGCTCGAGTTTAC
O314pTight-R(BS-AgeI-3)GCAACCGGTctgcagcggccgctactagtaGGCGATCTGACGGTTCACTAAAC
O315TRE3GV-F(3-BP) GCAgaattcgcggccgcttcTAGAGCCCTTTCGTCTTCACTCGAGTTTAC
O316TRE3GV-R(BS-AgeI-2)CAACCGGTctgcagcggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc
O317minCMV-F(3-BP)GCAgAATTCGCGGCCGCTTCTAGAGGGTAGGCGTGTACGGTGG
O318minCMV2-F(3-BP)GCAgaattcgcggccgcttcTAGAGGGTAGGCGTGTACGGTGG
O319pSB1C3-VF(S)TGCCACCTGACGTCTAAGAA
O320pSB1C3-VR(S)ATTACCGCCTTTGAGTGAGC
O321prefix-mNotch(e)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGCGTCGGTAGCA
O322mNotch(e)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTACCGTCGGCGCTTTCG
O323prefix-hNotch(e)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGCCCTGTCTTGGAGGGAATCC
O324hNotch(e)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTACCGGCGTCGTTTTCGAC
O325pSB1C3-FGCAGCATACTAGTAGCGGCCGCTG
O326pSB1C3-RGCAGCACTCTAGAAGCGGCCGCG
O327prefix-LaG17(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGTGAACCGTCAGATCAGATCCGCTAG
O328LaG17(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCA
O329prefix-CAR3G(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGCCAGCCAAACCAACAACTACAC
O330CAR3G(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccAAGCT
O331prefix-EGFPtm(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGTGGAAGAAAATCCCGGTCCG
O332EGFPtm(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct
O333prefix-tTAA(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGTGTCACGAAAGCGCCG
O334tTAA(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagct
O335IL-2(CO)-F(G_pSB1C3)GAAGATCTCACGACGtgaaccgtcagatccgctag
O336IL-2(CO)-R(G_pSB1C3)GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC
O337IL-10(CO)-F(G_pSB1C3)GAAGATCTCACGACGtgaaccgtcagatccgctag
O338IL-10(CO)-R(G_pSB1C3)GATGATCATACGACGATACGAAGTTATATTAAGGGTTCCAAGC
O339LaG16-2(CO)-F(G_pSB1C3)GAAGATCTCACGACGtgaaccgtcagatccgctag
O340LaG16-2(CO)-R(G_pSB1C3)GATGATCATACGACGGGGTTGCTACCGACGC
O341prefix-CD47tm(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc
O342CD47tm(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC
O343prefix-hYP9 1scFv(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc
O344hYP9 1scFv(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC
O345prefix-LaG 16-2(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O346LaG 16-2(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC
O347prefix-OKT3scFv(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGGGTGGCGGGGGGTC
O348OKT3scFv(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagcTTC
O349prefix-Tet-On3G(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGGGAGGTgaattcGCCACC
O350Tet-On3G(CO)-R -suffixGCAGCACTGCAGCGGCCGCTACTAGTAACCTCCggatccTTAGCC
O351prefix-α-CD19(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O352α-CD19(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGCAG
O353prefix-α-CD47(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O354α-CD47(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAGTAGTTGTTGGTTTGGCTGGC
O355prefix-IL-2(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc
O356IL-2(CO)-R-suffixGCAGCACTGCAGCGGCCGCTACTAGTAATACGAAGTTATATTAAGGGTTCCAAGC
O357IL-10(CO)-FGCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC
O358IL-10(CO)-RGCAGCACTGCAGCGGCCGCTACTAGTAatacgaagttatattaagggttccaagctTC
O359pML2-C0(AgeI) tgaaccgtcagatccgctagc
O360pML2-C0(HindIII)atacgaagttatattaagggttccaagc
O361mNc-F ATACTCGACTACAGTTTTACTGGGG
O362LaG2-R(OE_mNc)CCCCAGTAAAACTGTAGTCGAGTATAACAGTGACTTGCGTTCCCTG
O363LaG17-R(OE_mNc)CCCCAGTAAAACTGTAGTCGAGTATGGAAACGGTCACCTGTGTACC
O364CD8SP-F(3-AgeI-Kozak) GCAACCGGTGCCACCATGGCCCTTCCTGTAACGG
O365PDGFRB-R(TGA-HindIII-3)GCAAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATGATC
O3822*ZF21-16-F(Scar)tactagattagaagtgaattagaagtga
O3832*ZF21-16-R(Scar)ctagtatcacttctaattcacttctaat
O384BP-BbsI-F(3) ctggaattcgcggccgcttctagaGAAGAC
O3853-BP-BbsI-R(Scar)ctagtaGTCTTCtctagaagcggccgcgaattccag
O386BS-3-F TACTAGTAGCGGCCGCTGCAGtcc
O387BS-R(3) ggaCTGCAGCGGCCGCTA
O388EGFP-F(myr-L1)ccaaggatgccagccagcggcgccgcGGTGGTGGAGGCTCAATGGTGAGCAAGGGCGAG
O389 O389 EGFP-F(myr-L2)TGAATTCGGATCCGCCACCatgggtagcaacaagagcaagcccaaggatgccagccagc
O3902*ZF21-16-A-F(A)tactagattagaagtgaattagaagtgatactag
O3912*ZF21-16-A-R(A)tcacttctaattcacttctaat
O3922*ZF21-16-B-F(A)attagaagtgaattagaagtga
O3932*ZF21-16-B-R(A)ctagtatcacttctaattcacttctaatctagta
O394BP-F(A) GATCCGaattcgcggccgcttctagaGAAGAC
O395BP-R(A) ctagtaGTCTTCtctagaagcggccgcgaattcg
O396BS-F(A) TAGCGGCCGCTGCAGA
O397BS-R(A) AGCTTCTGCAGCGGCCGCTActagta
O398BP-F Cgaattcgcggccgc
O399BS-R TCTGCAGCGGCCGC
O400pSB1A3-C(3-BP-BamHI) GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg
O401pSB1A3-C(3-BS-HindIII C)GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg
O404mNCe-F(M) CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG
O405mNCe-F CCTTGTGTTGGtAGTAATCCATGCTAC
O406mNC|e-R(M) cctTCTtcttTTTCGTGACAGCAAAACACCGCAGCCTAC
O407mNC|e-R cctTCTtcttTTTCGTGACAGCAAAAC
O408LaG2-R(G_mNCe)ggattactaccaacacaaggAACAGTGACTTGCGTTCCCTGC
O409LaG17-R(G_mNCe)ggattactaccaacacaaggGGAAACGGTCACCTGTGTACC
O410tTAA-F(G_mNCe)TGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O411pML2-CX-R(Lin)CACCGGTaActcaagATgatctgacTAttcactacagctctgcttatatagacctccc
O412pML2-CX-F(Lin)TCAATTGGGAACTCGATACCTTCTAATATCTCATAataacttcgtataatgtatgctatacgaagttattaggtccctcgacctgctg
O413MCS-F(A) TGAATTCGGATCCAGGAGCAGTCTTCGGGCCCGAAGACTGCCATAGTCGACAAGCTTC
O414MCS-R(A) GAAGCTTGTCGACTATGGCAGTCTTCGGGCCCGAAGACTGCTCCTGGATCCGAATTCA
O415pCMV-F2(S) cccattgacgcaaatgggc
O416CD8aSP-F(Kozak G_pML2-CX)gtgaaTAgtcagatcATcttgagTtACCGGTGAATTCGCCACCATGGCGCTGCCGG
O417LaG2-R(G_mNCe) ATTaTAGCAaGGATTACTACCAACACAAGGAACAGTGACTTGCGTTCCCTGC
O418mNCe-F(M) CCTTGTGTTGGTAGTAATCCtTGCTAtAATCAAGGGACCTGCGAGC
O419mNC-F(M) ATACTCGACTAtAGTTTTACaGGGGGGGCGGGCCGGGATATACCCCCG
O420mNC|e-R(M) CCTTCTTCTTTTTCGTGACAGCAAAACtCCGCAGCCTACGAAGAACAAGAGTAC
O421tTAA-F(M) GGaGTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGGATAAATCTAAGG
O422tTAA-R(G_pML2-CX) GAGATATTAGAAGGTATCGAGTTCCCAATTGAAGCTTTCATCCTGGGAGCATATCCAGG
O423pML2-R(Lin L1)tcaagATgatctgacTAttcactacagctctgcttatatagacctcccaccgtacacgc
O424pML2-R(Lin L2)CGAATTCACCGGTaActcaagATgatctgacTAttcactacagctc
O425pML2-F(Lin L2)CAAGCTTCAATTGGGAACTCGATACCTTCTAATATCTC
O426pML2-CX(EcoRI) gtagtgaaTAgtcagatcATcttgagTtACCGGTG
O427pML2-CX(HindIII)TATGAGATATTAGAAGGTATCGAGTTCCCAATTGAAGC
O428pML2X-R(Lin1)AAGACCACCGGTGAATTCattaagggttccggttccactaggtacccacc
O429pML2X-R(Lin2)CGTCGACTAGCCCTAACGTGATCTGACAGTACATTAAGACCACCGGTGAATTCattaag
O430pML2X-F(Lin1)TGAtaggtccctcgacctgctggaatctcgtg
O431pML2X-F(Lin2)GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAtaggtccctcgacctgc
O432MCS-F(A) AGTCGACGGATCCAGGAGCAGTCTTCCTGCAGGAAGACTGCCATACAATTGAAGCTTC
O433MCS-R(A) GAAGCTTCAATTGTATGGCAGTCTTCCTGCAGGAAGACTGCTCCTGGATCCGTCGACT
O434pML2X-F(S) atcctccctttatccagccc
O435pML2X-R(S) atcgataagctcgcttcacg
O436pML2Y-F(Lin1) CGGCCGCtaggtccctcgacctgctggaatctcgtg
O437pML2Y-F(Lin2) GAAGCTTCCCTCATTCAAGTACCAGTCTAGTCGAAGTGTGAGCGGCCGCtaggtccctc
O438pML2Y(G_SalI) GGTCTTAATGTACTGTCAGATCACGTTAGGGC
O439pML2Y(G_HindIII)TCACACTTCGACTAGACTGGTACTTGAATGAGG
O440pML2X-R(Lin2)CGTCGACTAGCCCTAACGTGATCTGACAGTACAaTAAGACCACCGGTGAATTCattaag
O441pML2Y(G_SalI)GGTCTTAtTGTACTGTCAGATCACGTTAGGGC
O442CMV-F(3-EcoRI)GCAGAATTCcgttacataacttacggtaaatggcccgcctggctg
O443pCMV-R(AgeI-3)GCAACCGGTagctctgcttatatagacctcccaccgtacacgcc
O444PGK-F(3-EcoRI) GCAGAATTCgggtaggggaggc
O445PGK-R(AgeI-G_pML2Y-SalI)CGTCGACTAGCCCTAACGTGATCTGACAGTACAATAAGACCACCGGTcgaaaggcccgg
O446CD8aSP-F(G_pML2Y) GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCG
O447P2A-R(G_CD8aSP) tacaggaagggccatCGGACCGGGATTTTCTTCCACGTC
O448CD8aSP-F(G_P2A) gaaaatcccggtccgATGGCCCTTCCTGTAACG
O449PDGFRBtr-R(G_pML2Y)TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGC
O450CD8aSP-F(G_pML2Y)GGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCG
O451tTAA-R(G_pML2Y) TCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATC
O452LaG17-R(G_mNCe)attatagcaaggattactaccaacacaaggGGAAACGGTCACCTGTGTACCTTGTCC
O453aCD19-R(G_mNCe)attatagcaaggattactaccaacacaaggTGATGAAACGGTTACGGATGTCCCTTGTC
O454LaG16-2-R(G_mNC)CGCCCCCCCTGTAAAACTATAGTCGAGTATAACAGTGACTTGCGTTCCCTGCC
O455LaG17-R(G_mNC)cgccccccctgtaaaactatagtcgagtatGGAAACGGTCACCTGTGTACCTTGTCC
O456aCD19-R(G_mNC)CGCCCCCCCTGTAAAACTATAGTCGAGTATTGATGAAACGGTTACGGATGTCCCTTGTC
O457CD19ECD-R(G_Myc)gatcaatttctgctcCTTCCATCCCCCCGTGCG
O458Myc-F(G_CD19ECD)acggggggatggaagGAGCAGAAATTGATCTCTGAAGAG
O459PGK-F(S)cggcattctgcacgcttc
O460LaG16-F(L1)acgccgccaggccgGAGCAGAAGCTGATCTCAGAGGAGGACCTGATGGCCCAGGTGCAG
O461LaG16-F(L2)ggccttaccagtgaccgccttgctcctgccgctggccttgctgctccacgccgccaggc
O462LaG16-F(L3)tgaaccgtcagatccgctagcgctaCCGGTatggccttaccagtgaccg
O463mNC|e-R(M)CCTTCTTCTTTTTCGTGACAGCAAAACTCCACAGCCCACAAAGAACAGG
O464pML2Y-R(C) ACCACCGGTGAATTCattaagggttccggttccactaggtacccaccaaattccggcgc
O465pML2Y-F(C) TGAGCGGCCGCtaggtccctcgacctgctggaatctcgtgaagcgagcttatcg
O466XXX-F(1-AgeI-pML2Y-SalI) CACCGGTGGTCTTATTGTACTGTCAGATCACGTTAGGGCTAGTCGACccggggtggtgc
O467XXX-R(HindIII-pML2Y-NotI-1)aGCGGCCGCTCACACTTCGACTAGACTGGTACTTGAATGAGGGAAGCTTcccggcggcg
O468ZF 21-16GCAGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaTTAGAAGTGaTACTAGTAGCGGCCGCTGCAG
O469EGFP-F(3-SalI-Kozak-ATG-BamHI) CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGGAG
O470EGFP/mCherry-R(L1) GCTTGCTTTAACAGAGAGAAGTTCGTGGCgccggagccCTTGTACAGCTCGTCCATGCC
O471EGFP-R(L2) TCAATTGAGGTCCCGGGTTTTCTTCCACGTCTCCTGCTTGCTTTAACAGAGAGAAGTTC
O472P2A-R(MfeI-BbsI-TGA-SbfI-BbsI-HindIII-3)GGAAGCTTAGTCTTCCTGCAGGTCATGGAAGACTCAATTGAGGTCCCGGGTTTTCTTCC
O473CD8aSP-F(3-SalI-Kozak)GCAGTCGACGCCACCatggccttaccagtgaccg
O474TRE3GV-F(3-EcoRI)GCAGAATTCGAGTTTACTCCCTATCagtgatagagaacgtatgaag
O475TRE3GV-R(AgeI-3)GCAACCGGTAAGTATAAGACAAAAGTGTTGTGGAATTGcTCC
O476mCherry-R(KOD)CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCCTGCAGGGAGGAGTCCTGGGTCAC
O477mCherry-F(KOD)CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCC
O478BP-F(M_3-EcoRI) GCAGAATTCcggccgcttcTAGAG
O479BS-R(M_1-PstI-AgeI-3)GCAACCGGTCTGCAGacggccgcTACTAGT
O480ZF 43-8GCAGAATTCGCGGCCGCTTCTAGaGAGTGAGGAcaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAG
O481HIST2H2BE-F(CE_pML2Y)ATCACGTTAGGGCTAGTCGACGCCACCATGcctgaaccggcaaaatcc
O482HIST2H2BE-R(CE_EGFP)tccttggagctggtgtacttggtgac
O483EGFP-F(CE_HIST2H2BE)aagtacaccagctccaaggaCccTccTgtGgcCacAATGGTGAGCAAGGGCGAG
O484P2A-R(CE_pML2Y) GTACTTGAATGAGGGAAGCTTAGTCTTCCTGCAGGTC
O485LifeAct-F(3-SalI)CTAGTCGACGCCACCATGGGCGTGGCCGAC
O486myr-F(3-SalI)CTAGTCGACGCCACCATGggtagcaacaagagcaagcccaaggatgccagccagc
O4871-BP-21-16-43-8-SpePI-1-F(A)CGAATTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTG
O4881-BP-21-16-43-8-SpeI-1-R(A)CACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAAGCGGCCGCGAATTCG
O4891-XbaI-21-16-43-8-SpeI-BS-1-F(A)GTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTGCAGC
O4901-XbaI-21-16-43-8-SpeI-BS-1-R(A)GCTGCAGCGGCCGCTACTAGTAgTCCTCACTCttCACTTCTAAtCtCTAGAC
O491p2Y(CE_EcoRI) accggaacccttaatGAATTC
O492p2Y(CE_AgeI) CAGTACAATAAGACCACCGGT
O493p2Y(CE_SalI) ATCACGTTAGGGCTAGTCGAC
O494p2Y(CE_HindIII)GTACTTGAATGAGGGAAGCTT
O495mNC-F(M) ATTCTCGACTACAGTTTCACAGGAGGAGCGGGCCGGGATATACCCCCG
O496tTAA-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCATCCTGGGAGCATATCCAGGTC
O497CD8aSP-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGG
O498LaG2-R(CE_mNC)GTGAAACTGTAGTCGAGAATAACAGTGACTTGCGTTCCC
O499LaG17-R(CE_mNC)GTGAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACC
O500aCD19-R(CD_mNC) GTGAAACTGTAGTCGAGAATTGATGAAACGGTTACGGATGTCC
O501PGK-F(CE_p2Y) accggaacccttaatGAATTCgggtaggggaggcgc
O502PGK-R(CE_p2Y) CAGTACAATAAGACCACCGGTcgaaaggcccggagatgag
O503CD8aSP-F2(CE_p2Y)ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTGCCGGTC
O504CD19ECD(CE_Myc) atttctgctcCTTCCATCCCCCCGTGC
O505Myf-F(CE_CD19ECD)gggatggaagGAGCAGAAATTGATCTCTGAAGAGGAC
O506PDGFRBtr-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTCATCTAGGTTTCTTCTGCCAAAGCATG
O507TetOn3G-F(CE_P2A)TGGAAGAAAACCCGGGACCTATGAGCCGACTGGACAAGAGC
O508TetOn3G-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTTACAATTGGCCCGGCAACATATCAAGATCAAAGTC
O509U105 pEGFP-C5CATGGTCCTGCTGGAGTTCGT
O510mCherry-R(KOD)CCGCGCAGCTTCACCTTGTAGATGAACTCGCCGTCTTGCAGGGAGGAGTCCTGGGTCAC
O511mCherry-F(KOD)CACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAAAAGACCATGGGCTGGGAGGCC
O512BP-F(M CE_p2Y-EcoRI)accggaacccttaatGAATTCcggccgcttcTAGAG
O513BS(M CE_p2Y-AgeI) CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT
O514BS-R(M CE_p2Y-AS) CCCTAACGTGATCTGACAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGT
O515PIP-F(CE_p2Y-AS-Kozak)ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCATGTCAAGGGGGGAGGTTAG
O516KRAB-R(CE_HindIII) GTACTTGAATGAGGGAAGCTTTTACACCAGCCAGGGTTCC
O517pSV40-F(CE_p2Y-EcoRI)accggaacccttaatGAATTCcggccgcttcTAGAGgtgtgtcagttagggtgtgg
O518ZF21-16-F(CE_p2Y-EcoRI) accggaacccttaatGAATTCcggccgcttcTAGAGaTTAGAAGTG
O519CE_P2A-R AGGTCCCGGGTTTTCTTCC
O520TetOn3G-F(CE_P2A-MfeI) TGGAAGAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGC
O521TetOn3G-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTC
O522TRE3GV-F(1-BP-M_p2Y) CGAATTCcggccgcttcTAGAGGAGTTTACTCCCTATCagtgatagagaacgtatgaag
O523TRE3GV-R(BS-M_p2Y-AgeI-1)GACCGGTCTGCAGacggccgcTACTAGTAAAGTATAAGACAAAAGTGTTGTGGAATTGc
O524ZF43-8-F(CE_p2Y M) accggaacccttaatGAATTCcggccgcttcTAGAGaGAGTGAGG
O525LaG16-F(CE-P2Y_SalI)ATCACGTTAGGGCTAGTCGACGCCACCATGGCGCTGCCGGTCaccgccttgctcctgc
O526ZF43-8-F(CE_P2A) TGGAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC
O527ZF43-8-R GGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtctgttc
O528G4S-SV40NLS-F(CE_SynTF-C)ATACAGGTGAGAAGGGCTCCGGAGGAGGAGGTAGTCCAAAAAAG
O529KRAB-R(CE_p2Y-HindIII) GTACTTGAATGAGGGAAGCTTTCACAATTGCACCAGCCAAGGTTCTTCTCccttttcc
O530PIP-F(CE_P2A) TGGAAGAAAACCCGGGACCTATGTCAAGGGGGGAGGTTAG
O531ZF43-8-F(CE_p2Y-SalI)ACTGTCAGATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGAG
O532HA-F(CE_pGEX)TTCCAGGGGCCCCTGGGATCCTACCCCTATGATGTACCAGATTACGC
O533Myc-R(CE_pGEX)CTCGAGTCGACCCGGGAATTCTCACAGGTCCTCTTCAGAGATCAATTTC
O534TetOn3G-F(CE_P2A-MfeI) GAAAACCCGGGACCTCAATTGATGAGCCGACTGGACAAGAGCAAGGTAATC
O535TetOn3G-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTTAGCCCGGCAACATATCAAGATCAAAGTCGTCG
O536CD8aSP-F(CE_P2A) GTCCGATGGCCCTTCCTGTAACGG
O537PDGFRBtr(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTCAGCGAGGTTTCTTTTGCCAGAG
O538T2A-F(CE_p2Y-SalI-BamHI) ATCACGTTAGGGCTAGTCGACGCAGGATCCggAtcAggTGAAGGTAGGGGGTCATTGC
O539hYP9.1bscFv-R(CE_OKT3scFV)GACTGTTGGAGTTGAACCTGACTACCTCCTCCACCCTTGATCTCAAGTTTGGTTCCTTG
O540OKT3scFv-F CAGGTTCAACTCCAACAGTCTGG
O541OKT3scFv-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTCTAGCGGTTTATTTCAAGTTTTGTGCC
O5422.3D11scFv-R(CE_CAR3G)GTAGTTGTTGGTTTGGCTGGCTTTATTTCGACTTTCGTGCCTCC
O543CAR3G-F CCAGCCAAACCAACAACTACAC
O544CAR3G-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTCACCGTGGTGGCAAAGCC
O545IL-10-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTCAGTTCCTTATTTTCATCGTCATATACGCTTC
O546LaG17-R(CE_hNC)GAAACTGTAGTCGAGAATGGAAACGGTCACCTGTGTACCTTGTCCC
O547hNC-F(M) ATTCTCGACTACAGTTTCGGTGGGGGAGCGGG
O548hNC|e-R(M) CCTTCTTCTTTTTCGTGACAGCAAAACTCCACACCCTACG
O549mTagBFP2-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACGCCACCATGGTGTCTAAGGGCGAAGAG
O550mTagBFP2-R(M1) CGTCCTCGTATGTGGTGACTCTCTCCCATGTGAAGCCC
O551mTagBFP2-F(M2) AGTCACCACATACGAGGACGGGGGCGTGCTGACCG
O552mTagBFP2-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTCAATTCAGCTTGTGCCCCAGTTTG
O553mNc F(CE/G_LNR-A [-])ATTCTCGACTACAGTTTCACAGGAGGAGCGTCACTTAACTTCAATGATCCGTGGAAG
O554mNc F(CE/G_LNR-ABLnkr[-])ATTCTCGACTACAGTTTCACAGGAGGAGCGTGCACCCAGTCCCTCCA
O555mNc F(CE_LNR-A[-]hlx3+)ATTCTCGACTACAGTTTCACAATCACGCCTGCGGTT
O556mNC-R(M_S2)GTTCGCTTTTgtcctCTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC
O557mNC-F(M_S2)CGAAGaggacAAAAGCGAACCTGTCGAACCGCC
O558mNC-R(M_S3)GCAAAAagaagagcagTACGAAGAACAAGAGTACGAACGCAGCAGC
O559mNC-F(M_S3)CGTActgctcttctTTTTGCTGTCACGAAAAAGAAGAAGGATGAGTCGATTGG
O560MycoP-FCACCATCTGTCACTCTGTTAA
O561MycoP-RGGAGCAAACAGGATTAGATAC
O562BP-ZF21-16-43-8-BSTTCGCGGCCGCTTCTAGaGaTTAGAAGTGaaGAGTGAGGAcTACTAGTAGCGGCCGCTG
O563CE ZF21-16-43-8 FTAAGGATGATTTCTGGAATTCGCGGCCGCTTc
O564CE ZF21-16-43-8 RttgcccttttttgccggaCTGCAGCGGCCGctac
O565mNC-R-N(S)CCTCTGTCAGTTGGCAGTC
O566pGEX usual F(S)GAGCTGCATGTGTCAGAGG
O567pGEX usual R(S)GCAAGCCACGTTTGGTG
O568d2EGFP-R(L1) CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTCTTGTACAGCTCGTCCATG
O569d2EGFP-R(L2) ATCCCGCTCTCCTGGGCACAAGACATGGGCAGCGTGCCATCATCCTGCTCCTCCACCTC
O570d2EGFP-R(L3) CACATTGATCCTAGCAGAAGCACAGGCTGCTGGGTGACGGTCCATCCCGCTCTCCTGG
O571d2EGFP-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTCTACACATTGATCCTAGCAGAAGC
O572CD8aSP-F(CE_p2Y G3)ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG
O573EGFP-R(CE_mN1ce) GGATTACTACCAACACAAGGCTTGTACAGCTCGTCCATGCC
O574mN1c-R(C) CCTTCTTCTTTTTCGTGACAGCAAAACTCCGC
O575p2Y-F(C_HindIII) AAGCTTCCCTCATTCAAGTACCAGTCTAGTCG
O576VP64-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTCAGagcatGtcCagGtcGaagtcatctagggcatcgg
O577Gal4-F(CE_m|hN1c) TCACGAAAAAGAAGAAGGATGAAGCTGCTGAGCAGCatc
O578ZF21-16-F(CE_m|hN1c)TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC
O579ZF42-10-F(CE_mN1c)TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCAC
O580ZF43-8-F(CE_mN1c) TCACGAAAAAGAAGAAGGatgGCAAGTCCTAGGGTGAGAACTGGTTCTAAGACACC
O581KRAB-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTCACACCAGCCAAGGTTCTTCGCccttttccagcc
O582rtTAA-F(CE_m|hN1c|e) TCACGAAAAAGAAGAAGGatgtctagactggacaagagc
O583rtTAA-R(CE_p2Y-HindIII)GTACTTGAATGAGGGAAGCTTTCAcccggggagcatgtcaag
O584mN1c-F-C(S) CCAGTTGCACCTCATGTACG
O585mTagBFP2-R(L1)CCTGCTCCTCCACCTCCGGCGGGAAGCCATGGCTCAACTTATTCAGCTTGTGCCCCAG
O586rtTAA-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACGCCACCatgtctagactggacaagagcaaag
O587rtTAA-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTttacccggggagcatgtcaag
O588VP64-R(MfeI CE_p2Y)GTACTTGAATGAGGGAAGCTTTCACAATTGtagcatatcCagatcaaagtcatctaggg
O589ZF21-16-F(CE_P2A) GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTGAGAACAGgttcTAAGACACCTCC
O590ZF42-10-F(M CE_p2Y)accggaacccttaatGAATTCcggccgcttcTAGAGaGACGCTG
O591CMV-F(BP_M)tGAATTCcggccgcttcTAGAGcgttacataacttacggtaaatg
O592CMV-R(BS_M)cACCGGTCTGCAGacggccgcTACTAGTAagctctgcttatatagacctc
O593TetO-R(BS_M)cACCGGTCTGCAGacggccgcTACTAGTATCTCTATCACTGATAGGGAgtaaactggat
O594EGFP-F(L1_palm)CaaaatgcggctgctgcttcgcccgggaCccaccCgtcgccaccATGGTGAGCAAGGGC
O595EGFP-F(L2_palm)Gggggtcgctgtgcctgtccccgtgtgatccgaaaagtgctggcaaaatgcggctgctg
O596palm-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACGCCACCatgcggggggggcacaaagggggtcgctgtgc
O597mCherry-F(CE_mN1c-SV40NLS)TCACGAAAAAGAAGAAGGCCAAAAAAGAAGAGAAAGGTAATGGTGAGCAAGGGCGAGG
O598mCherry-R(L_HA) AGCGTAATCTGGTACGTCGTATGGGTACTTGTACAGCTCGTCCATGC
O599HA-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCAAGCGTAATCTGGTACGTCGTATG
O600mCherry(CE_mN1c)TCACGAAAAAGAAGAAGGATGGTGAGCAAGGGCGAGG
O601mN1c-R(M_S1)TCGAGTTCTGCAGCTGCAGCGCCACCACTCGTGCCCGG
O602mN1c-F(M_S1)GCTGCAGCTGCAGAACTCGATCCTATGGACATACGGGGGTCC
O603mN1c-R(M_S2)GTTCGCTTTTGTGCACTTCGATTTTATACGGAATATTCAAGCTCCCAAGAGAGGCC
O604mN1c-F(M_S2)CGAAGTGCACAAAAGCGAACCTGTCGAACCGCC
O605CD8aSP-F(CE_p2Y) ATCACGTTAGGGCTAGTCGACGCCACCATGGCCCTTCCTGTAACGG
O606PDGFRB-R GCGAGGTTTCTTTTGCCAG
O607mCherry-F(CE_PDGFRB)TGGCAAAAGAAACCTCGCATGGTGAGCAAGGGCGAGG
O608mCherry-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCACTTGTACAGCTCGTCCATGC
O609tTAA-F(CE_PDGFRB)TCTGGCAAAAGAAACCTCGCATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O610minCMV-R(L1_ZF21-16)cgcTACTAGTAtCACTTCTAAttCACTTCTAAtCTCTAgTAggcgatctgacggttcac
O611minCMV-R(L2_ZF21-16)CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAtCACTTCTAAttCAC
O612minCMV-R(L1_ZF43-8)cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAGGCGATCTGACGGTTCAC
O613minCMV-R(L2_ZF43-8)CAGTACAATAAGACCACCGGTCTGCAGacggccgcTACTAGTAgTCCTCACTCtg
O614tTAA-R-N(S)CCCCTCGATACCTACTTCGTTC
O615UAS-R(CE_minCMV）CTCTAGTActagagtctccgctcggaggacaga
O616minCMV-F(CE_UAS)cggagactctagTACTAGAGGGTAGGCGTGTACGGTGG
O617pSV40-R(L_ZF43-8)cgcTACTAGTAgTCCTCACTCtgTCCTCACTCtCTCTAgTAtttgcaaaagcctaggcc
O618mNc F (OE-test)ATTCTCGACTACAGTTTCACAGGAG
O619mNc R (OE-test)CCTTCTTCTTTTTCGTGACAGCAAAA
O620LNRA-RGCAGTCACCCCCGTCC
O621LNRB-FTGCACCCAGTCCCTCCAAT
O622LNRA R (OE_AB lnkr-WT10)GTTCTTCCACGGATCATTGAAGTTAAG
O623LNRB F (OE_AB lnkr-WT10)TCACTTAACTTCAATGATCCGTGGAAG
O624LNR A R (OE_ABlnkr-WT 8)CTTCCACGGATCATTGAAGTTAAGTG
O625LNR B F (OE_AB lnkr-WT8)CTTAACTTCAATGATCCGTGGAAGAAC
O626LNR A R (OE_AB lnkr-WT6)CCACGGATCATTGAAGTTAAGTGAG
O627LNR B F (OE_AB lnkr-WT6)AACTTCAATGATCCGTGGAAGAAC
O628LNR A R (OE_AB lnkr-WT4)CGGATCATTGAAGTTAAGTGAGCAG
O629LNR B F (OE_AB lnkr-WT4)TTCAATGATCCGTGGAAGAACTGC
O630LNR A R (OE_AB lnkr-WT2)ATCATTGAAGTTAAGTGAGCAGTCACC
O631LNR B F (OE_AB lnkr-WT2)AATGATCCGTGGAAGAACTGCAC
O632LNR A-lnkr R(OE mut L-I )AGTTCTTCCACGGATCATTGAAGTTGATTGAGCAGTCACCCCCGT
O633LNR B-lnkr F(OE mut L-I )ACGGGGGTGACTGCTCAATCAACTTCAATGATCCGTGGAAGAACT
O634LNR A-lnkr R(OE mut L-V)AGTTCTTCCACGGATCATTGAAGTTCACTGAGCAGTCACCCCCGT
O635LNR B-lnkr F(OE mut L-V )ACGGGGGTGACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACT
O636LNR A-lnkr R(OE mut L-A)AGTTCTTCCACGGATCATTGAAGTTGGCTGAGCAGTCACCCCCGT
O637LNR B-lnkr F(OE mut L-A )ACGGGGGTGACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACT
O638LNR A-lnkr R(OE mut L-G)AGTTCTTCCACGGATCATTGAAGTTGCCTGAGCAGTCACCCCCGT
O639LNR B-lnkr F(OE mut L-G )ACGGGGGTGACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACT
O640LNR A-lnkr R(OE mut S-G)GTTCTTCCACGGATCATTGAAGTTAAGGCCGCAGTCACCCCCGTCC
O641LNR B-lnkr F(OE mut S-G )GGACGGGGGTGACTGCGGCCTTAACTTCAATGATCCGTGGAAGAAC
O642LNR A-lnkr R(OE mut N-Q)GCAGTTCTTCCACGGATCATTGAATTGAAGTGAGCAGTCACCCCC
O643LNR B-lnkr F(OE mut N-Q )GGGGGTGACTGCTCACTTCAATTCAATGATCCGTGGAAGAACTGC
O644LNR A-lnkr R(OE mut N-V)GCAGTTCTTCCACGGATCATTGAAAACAAGTGAGCAGTCACCCCC
O645LNR B-lnkr F(OE mut N-V )GGGGGTGACTGCTCACTTGTTTTCAATGATCCGTGGAAGAACTGC
O646LNR A-lnkr R(OE mut S-A)GTTCTTCCACGGATCATTGAAGTTAAGGGCGCAGTCACCCCCGTCC
O647LNR B-lnkr F(OE mut S-A )GGACGGGGGTGACTGCGCCCTTAACTTCAATGATCCGTGGAAGAAC
O648IL10-F(L1)TGCTTACATGCGGAGATGTGGAGGAGAATCCGGGACCAATGCATTCTAGCGCTCTTTTG
O649IL10-F(L2)GAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTG
O650TetOn3G-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACGCCACCATGAGCCGACTGGACAAG
O651mNc F(CE_LNR-A[-]hlx3+)√ATTCTCGACTACAGTTTCACAAATCACGCCTGCGGTT
O652LaG17 R(CE_G6-LNR-A[-]hlx3+)ACCGCCTCCACCGCCTCCGGAAACGGTCACCTGTGTACC
O653mNc F(CE_G6-LNR-A[-]hlx3+)GGAGGCGGTGGAGGCGGTAATCACGCCTGCGGTTG
O654mNc F(CE_G6-LNR-A[-])GGAGGCGGTGGAGGCGGTTCACTTAACTTCAATGATCCGTGGAAG
O655LNR A-lnkr R(OE mut L-I )TTGAAGTTAAGTGAGCAGTCACCCCCGTCCCAAC
O656LNR B-lnkr F(OE mut L-I )GACTGCTCACTTAACTTCAATGATCCGTGGAAGAACTGCACCC
O657LNR A-lnkr R(OE mut L-V)TTGAAGTTCACTGAGCAGTCACCCCCGTCCCAAC
O658LNR B-lnkr F(OE mut L-V )GACTGCTCAGTGAACTTCAATGATCCGTGGAAGAACTGCACCC
O659LNR A-lnkr R(OE mut L-A)TTGAAGTTGGCTGAGCAGTCACCCCCGTCCCAAC
O660LNR B-lnkr F(OE mut L-A )GACTGCTCAGCCAACTTCAATGATCCGTGGAAGAACTGCACCC
O661LNR A-lnkr R(OE mut L-G)TTGAAGTTGCCTGAGCAGTCACCCCCGTCCCAAC
O662LNR B-lnkr F(OE mut L-G )GACTGCTCAGGCAACTTCAATGATCCGTGGAAGAACTGCACCC
O663LNR A-lnkr R(OE mut S-G)TTGAAGTTAAGGCCGCAGTCACCCCCGTCCCAACC
O664LNR B-lnkr F(OE mut S-G )GACTGCGGCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC
O665LNR A-lnkr R(OE mut N-Q)TTGAACTGAAGTGAGCAGTCACCCCCGTCC
O666LNR B-lnkr F(OE mut N-Q )GACTGCTCACTTCAGTTCAATGATCCGTGGAAGAACTGCACCCAG
O667LNR A-lnkr R(OE mut N-V)TTGAACACAAGTGAGCAGTCACCCCCGTCC
O668LNR B-lnkr F(OE mut N-V )GACTGCTCACTTGTGTTCAATGATCCGTGGAAGAACTGCACCCAG
O669LNR A-lnkr R(OE mut S-A)TTGAAGTTAAGGGCGCAGTCACCCCCGTCCCAACC
O670LNR B-lnkr F(OE mut S-A )GACTGCGCCCTTAACTTCAATGATCCGTGGAAGAACTGCACCC
O671LNR A-lnkr R(OE mut N-L)TTGAACAGAAGTGAGCAGTCACCCCCGTCC
O672LNR B-lnkr F(OE mut N-L )GACTGCTCACTTCTGTTCAATGATCCGTGGAAGAACTGCACCCAG
O673LNR A-lnkr R(OE mut N-G)TTGAAGCCAAGTGAGCAGTCACCCCCGTCC
O674LNR B-lnkr F(OE mut N-G )GACTGCTCACTTGGCTTCAATGATCCGTGGAAGAACTGCACCCAG
O675tTAA-F(CE_p2A)GAAGAAAACCCGGGACCTATGAGTCGATTGGATAAATCTAAGGTAATTAACAG
O676rtTAA-F(CE_p2A)GAAGAAAACCCGGGACCTatgtctagactggacaagagcaaag
O677CD19ECD-F(3-BamHI) CTGGGATCCGAAGAGCCTCTCGTCGTGAAAG
O678CD19ECD-R(TAA-EcoRI-3)CGGGAATTCTTACTTCCATCCCCCCGTGC
O679Gal4-R(CE_VP64)agaAcctccActTccAccagcagctgcCACTGTCAGCTGCCGCTGgc
O680VP64-F(CE_Gal4)ggTggAagTggaggTtctggCgggAGcgatgctttagacgattttgacttagatatgc
O681VP64-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTCAtgatccGagcatGtcCagGtcGaagtcatctaggg
O682Gal4-F(CE_P2A)GAAGAAAACCCGGGACCTATGAAGCTGCTGAGCAGCatc
O683mCherry-F-C(S)ACCTCCCACAACGAGGAC
O684SynTF-F-N(CE_P2A)GAAGAAAACCCGGGACCTatgGCAAGTCCTAGGGTG
O685mScarlet-F(3-SalI-Kozak-ATG-BamHI)CTAGTCGACGCCACCATGGGATCCGTGAGCAAGGGCGAGG
O686mScarlet-R(M) CTTGCGGTCcACGTTGTAGGCGCCG
O687mScarlet-F(M) TACAACGTgGACCGCAAGTTGGACATCAC
O688G4S-R(C) ACATCGGCCATTGAACCACCCCCACCGGAACC
O689LaG17-F(CE_G4S)TGGTTCAATGGCCGATGTGCAGC
O690mN1c-F-N(C) ATTCTCGACTACAGTTTCACAGGAGGAGCG
O691HA-R(C) CGCGTAATCTGGTACATCATAGGGGTAGGG
O692LaG17-F(CE_HA)GATGTACCAGATTACGCGATGGCCGATGTGCAGC
O693LaG17-R GGAAACGGTCACCTGTGTACC
O694G4S-F(CE_LaG17)ACACAGGTGACCGTTTCCGGGGGGGGTGGCAGTGG
O695SynZF-F(CE_p2Y-Kozak)ATCACGTTAGGGCTAGTCGACGCCACCATGGCAAGTCCTAGGGTG
O696TRE3GV-F(1-BP_M_p2Y)CGAATTCcggccgcttcTAGAGTCCCTATCagtgatagagaacgtatgaag
O6974xUAS-F(1-BP_M_p2Y)tGAATTCcggccgcttcTAGAGcgccaagctacggg
O6984xUAS-R(1-BS_M_p2Y)cACCGGTCTGCAGacggccgcTACTAGTAcgctcggaggacaga
O699CMV-R(L1_21-43)TCTAAtCTAGTAgTCCTCACTCttCACTTCTAAtCTAGTAagctctgcttatatagacc
O724pol-F-C GCCCTCAAAGTAGACGGC
O725ori-F-3CGCCTGGTATCTTTATAGTCCTG
O726tTAA-R(C) TCCTGGGAGCATATCCAGGTCAAGATCGTC
O727CD8ASP-F(L1_P2A) CTCCCTGCTGAAGCAAGCAGGAGACGTGGAAGAAAACCCGGGACCTATGGCGCTGCCGG
O728CD8ASP-F(L2_P2A CE_tTAA)CTGGATATGCTCCCAGGAggctccggcGCCACCAACTTCTCCCTGCTGAAGCAAGC
O729LaG2-F-N(S) GTGCTGCCTCCGGTC
O730tTAA-F-C(S) GCTTAAGTGCGAGAGTGGC
O731ACD8b-F-N(S) TATCCGGGGTCTCCCTCC
O732Gal4DBD-R-N(S) ttggcgcacttgggc
O733TetOn3G-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACGGAGGTgaattcGCCACC
O734TetOn3G-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTACCTCCggatccTTAGCC
O735T2A-F(CE_p2Y) ATCACGTTAGGGCTAGTCGACggAtcAggTGAGGGTCGG
O7362.3D11scFv-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTCACTTTATTTCGACTTTCGTGCCTCC
O737CAR3G-F(CE_p2Y)ATCACGTTAGGGCTAGTCGACCCAGCCAAACCAACAACTACAC
O747CD8A-F(CE_P2A)        GAAGAAAACCCGGGACCTATGGCGCTGCCGG
O748SynPro_space_5-F(CE_p2Y)accggaacccttaatGAATTCCGGGTTTCGTAACAATCGCATGAGGATTCGCAACGC
O749SynPro_space_7/7-F(CE_p2Y)accggaacccttaatGAATTCGCCTAGATCATCGTTGGC
O750SynPro_space_6/7-F(CE_p2Y)accggaacccttaatGAATTCCGCACGTATCACTTGATC
O751SynPro_space_5/7-F(CE_p2Y)accggaacccttaatGAATTCCTTTCCACGATCATGTGC
O752SynPro_space_4/4-F(CE_p2Y)accggaacccttaatGAATTCGACTGTTGCGAACGATTC
O753SynPro_space_3/4-F(CE_p2Y)accggaacccttaatGAATTCTCCCGTCTCAGTAAAGG
O754SynPro_space_2/4-F(CE_p2Y)accggaacccttaatGAATTCAATCGGACTGCCTTCG
O755SynPro_space_1/4-F(CE_p2Y)accggaacccttaatGAATTCGTATCAGTCGCCTCGG
O756minCMV2-F(L_space3) CGTAAGAGGCTCACTCTCCCTTACACGGAGTGGATAGGTAGGCGTGTACGG
O757minCMV2-R(CE_p2Y) CAGTACAaTAAGACCACCGGTAAGTATAAGACAAAAGTGTTGTGG
O758ZF21.16_8/8-F(L) GAGGATTCGCAACGCaTTAGAAGTGaGCCTAGATCATCGTTGGC
O759ZF21.16_7/8-F(L) GCCTAGATCATCGTTGGCaTTAGAAGTGaCGCACGTATCACTTGATC
O760ZF21.16_6/8-F(L) CGCACGTATCACTTGATCaTTAGAAGTGaCTTTCCACGATCATGTGC
O761ZF21.16_5/8-F(L) CTTTCCACGATCATGTGCaTTAGAAGTGaGACTGTTGCGAACGATTC
O762ZF21.16_4/8-F(L) GACTGTTGCGAACGATTCaTTAGAAGTGaTCCCGTCTCAGTAAAGG
O763ZF21.16_3/8-F(L) TCCCGTCTCAGTAAAGGTaTTAGAAGTGaAATCGGACTGCCTTCG
O764ZF21.16_2/8-F(L) AATCGGACTGCCTTCGTAaTTAGAAGTGaGTATCAGTCGCCTCGG
O765ZF21.16_1/8-F(L) GTATCAGTCGCCTCGGAAaTTAGAAGTGaCGTAAGAGGCTCACTCTC
O766TetO_6/6-F(L)CGCACGTATCACTTGATCTCCCTATCagtgatagagaCTTTCCACGATCATGTGC
O767TetO_5/6-F(L)CTTTCCACGATCATGTGCTCCCTATCagtgatagagaGACTGTTGCGAACGATTC
O768TetO_4/6-F(L)GACTGTTGCGAACGATTCTCCCTATCagtgatagagaTCCCGTCTCAGTAAAGG
O769TetO_3/6-F(L)TCCCGTCTCAGTAAAGGTTCCCTATCagtgatagagaAATCGGACTGCCTTCG
O770TetO_2/6-F(L)AATCGGACTGCCTTCGTATCCCTATCagtgatagagaGTATCAGTCGCCTCGG
O771TetO_1/6-F(L)GTATCAGTCGCCTCGGAATCCCTATCagtgatagagaCGTAAGAGGCTCACTCTC
O772minCMV2-F(CE_p2Y)accggaacccttaatGAATTCGGTAGGCGTGTACGGTGG
O773SV40NLS-KRAB-F(CE_P2A)GAAGAAAACCCGGGACCTATGGGCCCAAAAAAGAAGAGAAAGGTAatggacg
O774KRAB-G4S-R ACTACCTCCTCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag
O775TetR-F(CE_KRAB-G4S) TTGTAGGTGGAGGAGGTAGTATGAGTCGATTGGATAAATCTAAGG
O776TetR-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCAAGAGCCACTCTCGCACTTAAG
O777Gal4-F(CE_KRAB-G4S)TTGTAGGTGGAGGAGGTAGTATGAAGCTGCTGAGCAGCatc
O778Gal4-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCACACTGTCAGCTGCCGC
O779SynZFN-F(CE_KRAB-G4S)TTGTAGGTGGAGGAGGTAGTATGGCAAGTCCTAGGGTG
O780ZF21.16-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGTGTTTTCAGATGc
O781ZF42.10-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTGTGGGTTTTCAGATGc
O782ZF43.8-R(CE_p2Y)GTACTTGAATGAGGGAAGCTTTCAGGAGCCCTTCTCACCTGTATGTGTTTTCAGATGtc
O7837xTetO-R(CE_CMVe)accgtaagttatgtaacgGCCTACCTCGACATACG
O7844xUAS-F(CE_p2Y)accggaacccttaatGAATTCcgccaagctacggg
O7854xUAS-R(CE_CMVe)accgtaagttatgtaacgGTActagagtctccgc
O7868xZFO-R(CE_CMVe)accgtaagttatgtaacgCACGCCTACCCTCTAG
O787VP64-F(CE_p2Y) ATCACGTTAGGGCTAGTCGACGCCACCATGgatgctttagacgattttgacttag
O788VP64-G4S-R ACTACCTCCTCCACCAagcatGtcCagGtcGaagtcatc
O789SynZF-F(CE_VP64_G4S)tgctTGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG
O790CfaN-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACCTG
O791KRAB-F(CE_p2Y) ATCACGTTAGGGCTAGTCGACGCCACCATGgacgccaagagcctg
O792SynZF-F(CE_KRAB_G4S)TTGTAGGTGGAGGAGGTAGTatgGCAAGTCCTAGGGTG
O793CfaC-F(CE_p2Y) ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGG
O794SynZF-R(SV40NLS-CE_p2Y)TACTTGAATGAGGGAAGCTTTCATACCTTTCTCTTCTTTTTTGGGGAGCCCTTCTCACC
O795KRAB-R(CE_NES) GTCAATCTTTCCAGGGGCGGCAGTACAAGCCACGGTTCCTCGCccttttccagcctaag
O796SynZF-F(L1_dNLS)GATCAGAGAACTTGTATTTTCAAAGTGGCAAGAAGAAGAAAATGGCAAGTCCTAGGGTG
O797SynZF-F(L2_dNLS)GCCCCTGGAAAGATTGACGCTGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC
O798KRAB-R TACAAGCCACGGTTCCTCGCccttttccagcctaag
O799dNLS-F(CE_KRAB)GAGGAACCGTGGCTTGTAAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC
O800KRAB-R(CE_TCS)CAAGTTCTCTGATCCACCTACAAGCCACGGTTCCTCGCccttttccagcctaag
O801SynZF-F(CE_TCS)GGTGGATCAGAGAACTTGTATTTTCAAAGTGGCGGAATGGCAAGTCCTAGGGTG
O802TEVp-F(L1_NLS)GGTGCCAAAAAAGAAGAGAAAGGTAGGGGAGAGCCTGTTCAAAG
O803TEVp-F(L2_NLS)ATCACGTTAGGGCTAGTCGACGCCACCATGGTGCCAAAAAAGAAGAGAAAGG
O804TEVpN-R(CE_CfaN)CGTGTCGTATGAGAGGCATGTCTGAAAATTAGTGGTTACCAAAC
O805CfaN-F TGCCTCTCATACGACACGGAAATTTTGACCGTC
O806CfaN-R(CE_p2Y) GTACTTGAATGAGGGAAGCTTTCATGGCAACCCATCGACC
O807CfaC-F ATCACGTTAGGGCTAGTCGACGCCACCATGGTTAAGATCATATCACGGAAGAGC
O808CfaC-R GTTTGAAGCGACGAGACCATTTTTAAGGAGG
O809TEVpC-F(CE_CfaC)GGTCTCGTCGCTTCAAACTGCGCCGAAAAGAGCATGTCCTCCATGG
O810BS-R(CE_minCMV2)ACGCCTACCTCGACATACGTCACGCCTACCCTCTAG
O811minCMV2-F ACGTATGTCGAGGTAGGCGT
O812VP64-R CagcatGtcCagGtcGaagtcatc
O813dNLS-F(CE_VP64)ttCgaCctGgaCatgctGAAGAGAGGATCAGAGAACTTGTATTTTCAAAGTGGC
O814SynZFC-R(CE_T2AN)CCTACCTTCAccTgaTccGGAGCCCTTCTCACCTGT
O815SV40NLS-F(L1_T2A) GCTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAGTGCCAAAAAAGAAGAGAAAGG
O816T2A-F ggAtcAggTGAAGGTAGGGGGTCATTGCTTACATGCGGAGATGTGG
O817TEVpC-R(NLS-CE_T2A)CCTACCTTCAccTgaTccTACCTTTCTCTTCTTTTTTGGCTGGCTATAGACCAATTCG
O818VP64-F(L_T2A) TACATGCGGAGATGTGGAGGAGAATCCTGGACCAgatgctttagacgattttgacttag
O819KRAB-F(L_T2A)CTTACATGCGGAGATGTGGAGGAGAATCCTGGACCAATGgacgccaagagcc
O824CE_p2Y-SalI(CE_pSB1C3-XbaI) gaattcgcggccgcttctagagATCACGTTAGGGCTAGTCGAC
O825CE_p2Y-HindIII(CE_pSB1C3-SpeI)actgcagcggccgctactagtaGTACTTGAATGAGGGAAGCTT
O826CE_p2Y-EcoRI(CE_pSB1C3-XbaI)gaattcgcggccgcttctagagaccggaacccttaatGAATTC
O827CE_p2Y-AgeI(CE_pSB1C3-SpeI)actgcagcggccgctactagtaCAGTACAaTAAGACCACCGGT
O828mN1c-F(CE_BP)gaattcgcggccgcttctagagATTCTCGACTACAGTTTC
O829mN1c-R(CE_BS)actgcagcggccgctactagtaCCTTCTTCTTTTTCGTGA
O830mN1ce-F(CE_BP)gaattcgcggccgcttctagagCCTTGTGTTGGTAGTAATCC
O831LaG2-F(CE_BP)gaattcgcggccgcttctagagATGGCTCAGGTCCAGCTTG
O832LaG2-R(CE_BS)actgcagcggccgctactagtaAACAGTGACTTGCGTTCCC
O833LaG16(CE_BP) gaattcgcggccgcttctagagATGGCACAGGTCCAACTCG
O834LaG16-R(CE_BS)actgcagcggccgctactagtaGCTTACCGTTACTTGTGTGCC
O835LaG17-F(CE_BP)gaattcgcggccgcttctagagATGGCCGATGTGCAGC
O836LaG17-R(CE_BS)actgcagcggccgctactagtaGGAAACGGTCACCTGTGTACC
O837ACD19-F(CE_BP)gaattcgcggccgcttctagagGATATACAAATGACCCAGACTACGTCC
O838ACD19-R(CE_BS)actgcagcggccgctactagtaTGATGAAACGGTTACGGATGTCC
O839CfaN-F(CE_BP)gaattcgcggccgcttctagagTGCCTCTCATATGACACGG
O840CfaN-R(CE_BS)actgcagcggccgctactagtaTGGCAACCCATCGACCTG
O841CfaC-F(CE_BP)gaattcgcggccgcttctagagATGGTTAAGATCATATCACGGAAGAGC
O842CfaC-R(CE_BS)actgcagcggccgctactagtaGTTTGAAGCTACGAGACCATTTTTAAGG
O843TEVN(CE_BP)gaattcgcggccgcttctagagGGGGAGAGCCTGTTCAAAGG
O844TEVpN(CE_BS)actgcagcggccgctactagtaTGTCTGAAAATTAGTGGTTACCAAACAAATG
O845TEVpC-F(CE_BP)gaattcgcggccgcttctagagTGCGCCGAAAAGAGCATGTCCTCCATGGTTAG
O846TEVpC-R(CE_BS)actgcagcggccgctactagtaCTGGCTATAGACCAATTCGTTCATC
O847SynZFN-F(CE_BP) gaattcgcggccgcttctagagatgGCAAGTCCTAGGGTG
O848ZF21.16N-R(CE_BS)actgcagcggccgctactagtaCTGGAAGGGCTTTTCTCCG
O849ZF21.16C-F(CE_BP)gaattcgcggccgcttctagagTGTAGGATATGCATGCGAAACTTCTC
O850SynZFC-R(CE_BS) actgcagcggccgctactagtaGGAGCCCTTCTCACCTGT

gBlocks

> alphaCD19(CO)
tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG
CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCTACCCCTATGATGTACCAGAT
TACGCGGATATACAAATGACCCAGACTACGTCCAGCCTTTCCGCCTCCCTGGGTGATCGC
GTGACAATCAGTTGTAGGGCTAGTCAGGACATAAGTAAATACCTCAACTGGTATCAACAG
AAGCCTGACGGAACAGTTAAACTTCTTATCTATCATACAAGTAGGCTTCATTCAGGCGTT
CCATCCAGATTTAGTGGATCTGGGAGCGGCACCGACTACTCACTCACGATTAGTAATCTG
GAACAAGAGGACATCGCCACTTATTTTTGCCAGCAGGGTAACACTCTTCCCTATACATTC
GGCGGCGGTACGAAACTCGAAATTACAGGGGGGGGTGGCAGTGGTGGCGGAGGTTCCGGT
GGGGGTGGTTCAGAAGTAAAACTTCAGGAATCTGGTCCAGGGCTGGTTGCACCCAGCCAA
TCCCTTTCCGTCACGTGCACCGTATCCGGGGTCTCCCTCCCAGACTACGGCGTGAGTTGG
ATACGACAGCCGCCGCGCAAGGGGCTTGAGTGGCTGGGTGTCATTTGGGGATCTGAAACA
ACGTATTATAATTCCGCTTTGAAATCCCGCCTCACTATTATCAAGGACAATTCTAAGAGC
CAGGTCTTTCTGAAGATGAACAGCCTGCAAACGGATGACACAGCCATCTACTATTGTGCA
AAGCACTATTACTATGGTGGTTCATACGCTATGGATTACTGGGGACAAGGGACATCCGTA
ACCGTTTCATCACCCTGCGTCGGTAGCAACCC
> tTAA(CO)
TGTCACGAAAGCGCCGACGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAGCGCAC
TGGAGCTTCTGAACGAAGTAGGTATCGAGGGGCTCACCACACGAAAATTGGCACAGAAGC
TCGGAGTGGAGCAGCCGACTTTGTACTGGCACGTTAAGAATAAGAGGGCTTTGCTTGACG
CGCTGGCCATTGAGATGCTCGACCGGCATCACACTCACTTCTGCCCGCTTGAAGGCGAGA
GTTGGCAAGATTTCCTTAGGAACAATGCAAAGAGCTTTCGATGCGCCCTGCTTAGCCATC
GGGACGGCGCAAAGGTTCATCTTGGGACTCGGCCTACTGAGAAGCAATATGAAACCCTGG
AAAACCAGCTTGCTTTCCTTTGCCAACAGGGGTTCTCACTGGAGAATGCCCTGTACGCAC
TCTCCGCGGTCGGCCATTTTACTCTCGGGTGTGTTTTGGAAGATCAGGAACACCAGGTAG
CGAAAGAGGAGCGAGAAACGCCTACAACTGATTCCATGCCACCGTTGCTGAGACAAGCTA
TAGAGTTGTTCGACCACCAGGGTGCTGAACCGGCGTTTCTCTTTGGTCTGGAACTCATTA
TCTGCGGCCTTGAAAAGCAGCTTAAGTGCGAGAGTGGCGGTCCCGCAGATGCACTTGATG
ACTTTGATTTGGATATGTTGCCCGCGGACGCTCTTGATGACTTCGATCTGGATATGCTGC
CGGCTGATGCCCTCGACGATCTTGACCTGGATATGCTCCCAGGATGAaagcttggaaccc
ttaatataacttcgtat
> TetOn3G(CO)
GGAGGTgaattcGCCACCATGAGCCGACTGGACAAGAGCAAGGTAATCAATTCAGCTTTG
GAGCTTCTGAACGGCGTGGGAATCGAGGGTCTCACCACCAGAAAGCTCGCGCAAAAATTG
GGGGTGGAACAACCGACTCTCTATTGGCATGTTAAGAACAAGAGGGCCCTCCTTGACGCA
CTCCCCATCGAAATGCTGGATCGACATCACACACATAGTTGTCCGCTGGAGGGGGAGTCT
TGGCAAGATTTCCTCAGGAATAACGCGAAGAGTTATCGGTGCGCTCTCCTTAGTCATCGC
GATGGAGCGAAAGTGCATCTGGGGACAAGACCGACGGAAAAACAATATGAGACGTTGGAG
AACCAACTGGCATTCTTGTGCCAACAGGGGTTCAGCCTTGAAAATGCTCTTTACGCCTTG
AGCGCTGTTGGGCACTTCACCTTGGGCTGTGTACTGGAGGAGCAGGAGCACCAGGTCGCA
AAGGAGGAGCGCGAAACGCCCACCACAGATAGTATGCCACCCCTGTTGAAGCAGGCTATC
GAGCTTTTTGATCGGCAGGGTGCTGAGCCTGCCTTTTTGTTCGGCCTTGAGCTTATAATT
TGCGGCCTGGAAAAACAACTCAAGTGCGAAAGCGGTGGTCCCACAGACGCATTGGACGAT
TTTGATCTTGACATGCTGCCAGCCGATGCTTTGGACGATTTTGACCTGGATATGTTGCCG
GCAGATGCCCTCGACGACTTTGATCTTGATATGTTGCCGGGCTAAggatccGGAGGT
> mN1ce(CO)
CCCTGCGTCGGTAGCAACCCCTGCTACAATCAAGGGACCTGCGAGCCAACGTCAGAAAAT
CCCTTTTATAGATGCCTGTGCCCGGCAAAGTTCAACGGACTGCTGTGCCACATACTCGAC
TACAGTTTTACTGGGGGGGCGGGCCGGGATATACCCCCGCCTCAGATAGAAGAGGCATGC
GAACTGCCGGAATGCCAGGTCGATGCGGGCAATAAAGTCTGCAACCTCCAATGCAATAAT
CACGCCTGCGGTTGGGACGGGGGTGACTGCTCACTTAACTTCAATGATCCGTGGAAGAAC
TGCACCCAGTCCCTCCAATGTTGGAAATATTTTTCCGATGGTCATTGTGATAGCCAGTGC
AATTCCGCAGGATGTTTGTTCGATGGGTTTGACTGCCAACTGACAGAGGGTCAGTGTAAT
CCTCTTTACGACCAATACTGCAAGGATCATTTCAGTGATGGACATTGTGACCAAGGTTGT
AACAGTGCGGAGTGCGAATGGGATGGGTTGGATTGTGCTGAGCATGTTCCTGAGCGCCTC
GCAGCGGGAACTTTGGTCCTCGTTGTCCTCCTTCCGCCTGACCAGCTCAGAAATAATAGC
TTTCACTTTCTTAGAGAACTCTCTCATGTGTTGCATACCAACGTAGTCTTTAAGCGCGAC
GCTCAAGGACAGCAAATGATCTTCCCGTACTATGGGCACGAAGAAGAGCTTCGGAAACAC
CCGATAAAAAGGAGTACGGTTGGTTGGGCGACGAGTAGTCTTTTGCCGGGCACGAGTGGT
GGCAGACAACGGCGGGAACTCGATCCGATGGACATACGGGGGTCCATAGTGTATCTGGAG
ATTGACAACCGACAGTGTGTTCAAAGTTCAAGCCAGTGTTTTCAATCAGCCACTGATGTA
GCTGCGTTTCTCGGAGCGTTGGCCTCTCTTGGGAGCTTGAATATTCCGTATAAAATCGAA
GCTGTAAAAAGCGAACCTGTCGAACCGCCATTGCCAAGCCAGTTGCACCTCATGTACGTG
GCCGCTGCTGCGTTCGTACTCTTGTTCTTCGTAGGCTGCGGTGTTTTGCTGTCACGAAAG
CGCCGACGG
> hN1c(CO)
CCCTGTCTTGGAGGGAATCCGTGTTACAACCAAGGGACATGTGAACCCACGAGCGAGTCT
CCTTTTTACAGGTGTCTCTGTCCAGCAAAATTTAATGGATTGCTTTGCCACATTCTGGAT
TACTCATTTGGTGGGGGAGCGGGGCGGGACATACCGCCGCCCCTTATTGAAGAAGCATGC
GAACTGCCCGAGTGCCAGGAGGATGCAGGTAACAAGGTATGCTCTCTTCAGTGCAATAAC
CACGCTTGCGGGTGGGATGGTGGCGATTGCTCCTTGAATTTTAATGATCCTTGGAAAAAT
TGCACCCAATCACTTCAGTGTTGGAAATACTTCTCAGATGGCCATTGCGATTCTCAGTGT
AACAGCGCGGGATGCTTGTTTGACGGATTCGACTGTCAAAGGGCTGAGGGACAATGTAAT
CCGCTCTACGATCAGTATTGTAAAGACCATTTCAGCGACGGCCACTGTGACCAAGGCTGC
AATAGTGCCGAGTGTGAGTGGGATGGGTTGGATTGCGCGGAGCATGTTCCGGAGAGACTT
GCGGCAGGTACGCTCGTGGTTGTTGTACTCATGCCGCCTGAGCAACTGAGGAATAGTAGC
TTTCACTTCCTCCGGGAACTGAGCCGGGTTCTGCATACTAACGTTGTATTTAAGAGAGAT
GCACATGGTCAGCAAATGATATTTCCGTACTACGGTCGGGAAGAGGAATTGCGGAAGCAC
CCCATTAAGAGAGCTGCCGAGGGTTGGGCCGCTCCGGATGCCCTCCTTGGACAAGTAAAA
GCCTCCCTCCTTCCCGGCGGATCTGAGGGTGGACGGAGACGCCGGGAACTCGATCCAATG
GATGTAAGAGGGTCCATTGTGTACTTGGAGATTGATAATCGCCAGTGTGTGCAGGCGTCC
TCACAATGTTTTCAAAGTGCGACCGATGTCGCAGCATTCTTGGGTGCCCTGGCATCATTG
GGCAGCCTGAATATTCCATATAAGATCGAAGCGGTGCAATCCGAAACGGTGGAACCGCCT
CCACCGGCACAGCTTCATTTCATGTATGTCGCAGCAGCAGCTTTCGTTTTGCTTTTCTTC
GTAGGGTGTGGTGTTCTCCTTAGTCGAAAACGACGCCGG
> LaG17(CO)
tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG
CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG
GAGGACCTCATGGCCGATGTGCAGCTCGTCGAGTCAGGTGGGGGTCTCGTACAAGCTGGG
GGGTCACTCAGACTGTCTTGCGCCGCCAGTGGCAGAACCATCTCTATGGCAGCTATGTCC
TGGTTCAGACAGGCGCCAGGCAAGGAGAGGGAGTTTGTGGCGGGTATAAGTAGGTCCGCA
GGAAGCGCGGTCCACGCCGATTCAGTCAAAGGTCGGTTCACGATAAGCCGGGATAACACT
AAGAACACTCTTTATCTCCAGATGAACTCTCTGAAGGCCGAGGATACGGCTGTTTACTAT
TGCGCTGTACGGACATCTGGGTTCTTCGGGAGCATACCGCGCACCGGAACAGCGTTTGAT
TACTGGGGACAAGGTACACAGGTGACCGTTTCCCCCTGCGTCGGTAGCAACCC
> LaG16-2(CO)
tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG
CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG
GAGGACCTCATGGCACAGGTCCAACTCGTCGAATCCGGAGGTCGCCTCGTGCAAGCTGGC
GATTCACTTCGACTCTCTTGCGCGGCGTCAGGTCGGACCTTCAGCACGTCAGCTATGGCG
TGGTTCCGCCAGGCGCCTGGGAGAGAGAGAGAGTTTGTAGCAGCCATCACATGGACTGTT
GGAAATACTATATTGGGCGACTCCGTAAAAGGGCGCTTCACTATCTCACGAGATAGAGCG
AAAAACACTGTAGATCTGCAAATGGACAATTTGGAGCCGGAGGATACTGCTGTGTATTAT
TGCAGCGCTCGCTCTCGGGGGTACGTCCTCTCCGTACTCCGGAGCGTGGATTCATACGAC
TACTGGGGACAGGGCACACAAGTAACGGTAAGCGGTGGGGGAGGGTCTGGTGGTGGAGGC
TCAGGAGGCGGGGGATCTATGGCTCAGGTCCAGCTTGTAGAGAGCGGTGGTGGATTGGTG
CAAGCAGGAGGGAGCCTCAGGCTTAGTTGTGCTGCCTCCGGTCGAACCTTCTCAAATTAT
GCAATGGGTTGGTTTAGGCAAGCTCCTGGTAAAGAACGGGAGTTCGTGGCCGCAATTAGC
TGGACGGGAGTTAGCACATATTACGCTGACTCTGTGAAAGGTCGGTTCACGATAAGTCGA
GACAATGATAAGAATACGGTTTACGTCCAAATGAATAGCCTCATCCCGGAGGACACAGCT
ATATATTATTGCGCTGCTGTACGGGCAAGATCCTTCTCAGATACTTATAGCAGGGTTAAC
GAATACGATTATTGGGGGCAGGGAACGCAAGTCACTGTTCCCTGCGTCGGTAGCAACCC
> EGFPtm(CO)
GGAAGAAAATCCCGGTCCGATGGCCCTTCCTGTAACGGCGCTCCTTCTCCCTCTTGCCC
TCCTGCTCCATGCCGCACGACCAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGG
TGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCG
AGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA
AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA
GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT
ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG
TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGG
AGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATA
TCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCG
AGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC
CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA
ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCG
GCATGGACGAGCTGTACAAGAATGCCGTTGGTCAAGACACGCAGGAAGTCATTGTTGTAC
CCCACTCCCTGCCATTTAAGGTGGTAGTAATTTCAGCCATCCTTGCTCTCGTTGTGTTGA
CAATTATTTCTCTGATAATTCTTATCATGCTCTGGCAAAAGAAACCTCGCTGAaagcttg
gaacccttaatataacttcgtat
> CD19ECD(CO)
TTGCTCCCACTGGCCTTGCTGCTTCATGCGGCACGGCCTTACCCCTATGATGTACCAGAT
TACGCGGAAGAGCCTCTCGTCGTGAAAGTTGAAGAAGGTGACAATGCAGTTTTGCAATGT
CTGAAAGGGACGTCTGACGGACCAACCCAACAGCTCACTTGGAGTCGCGAATCCCCGCTT
AAGCCGTTCCTGAAGTTGTCTCTGGGGTTGCCGGGTCTTGGTATACACATGCGCCCGCTT
GCGATATGGCTCTTCATCTTCAATGTCAGCCAACAGATGGGCGGGTTCTATCTCTGTCAA
CCGGGACCGCCATCAGAGAAGGCGTGGCAACCTGGTTGGACGGTAAACGTGGAAGGTAGC
GGGGAGTTGTTTCGCTGGAACGTTTCTGATCTCGGGGGCCTGGGTTGCGGTCTGAAAAAT
CGCTCTAGCGAAGGGCCGAGTTCACCTTCCGGCAAGCTCATGTCCCCGAAACTCTACGTG
TGGGCGAAGGATAGGCCAGAAATTTGGGAGGGAGAACCCCCCTGCCTGCCGCCCAGAGAT
TCACTCAACCAGAGTCTCTCCCAAGATCTGACTATGGCCCCAGGAAGTACCTTGTGGCTT
AGCTGCGGTGTACCGCCCGACTCCGTGAGCCGAGGGCCTCTCTCTTGGACACACGTTCAC
CCTAAGGGGCCGAAATCATTGTTGAGTTTGGAACTCAAAGACGATAGGCCAGCAAGGGAT
ATGTGGGTTATGGAGACCGGCCTTCTGCTCCCACGAGCGACCGCTCAGGATGCGGGCAAG
TATTATTGCCACAGAGGAAATCTGACTATGTCTTTTCATCTGGAAATTACTGCGCGGCCA
GTTTTGTGGCACTGGCTGTTGCGCACGGGGGGATGGAAGGAGCAGAAATTGATCTCTGA
> Gal4DBD(CO)
atgaagctgctgagcagcatcgagcaggcctgtgacatctgccggctgaagaaactgaag
tgcagcaaagaaaagcccaagtgcgccaagtgcctgaagaacaactgggagtgccggtac
agccccaagaccaagagaagccccctgaccagagcccacctgaccgaggtggaaagccgg
ctggaaagactggaacagctgtttctgctgatcttcccacgcgaggacctggacatgatc
ctgaagatggacagcctccaggacatcaaggccctgctgaccggcctgttcgtgcaggac
aacgtgaacaaggacgccgtgaccgacagactggccagcgtggaaaccgacatgcccctg
accctgcggcagcacagaatcagcgccaccagcagcagcgaggaaagcagcaacaagggc
cagcggcagctgacagtgtct
> ZF21.16(CO)
GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCTCCTCACGAGAGGCCTTTTCAATGT
AGAATTTGTATGCGTAATTTTTCTaggaacttcatcctccagagaCATACAAGGACTCAC
ACAGGTGAAAAGCCTTTTCAGTGCAGAATTTGTATGCGCAATTTTTCTcagggcggtaac
ctggtgagaCATCTGCGTACCCACACTGGTGAAAAGCCTTTCCAATGTAGAATTTGTATG
CGAAATTTTTCTcagcaaaccggcctgaacgtgCATCTGAAGACACACACAGGCGAGAAG
GGATCC
> ZF42.10(CO)
GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCACCACATGAAAGACCATTCCAATGT
AGAATTTGTATGAGGAATTTTTCTaccggccagatcctggacagaCATACAAGGACCCAC
ACCGGCGAAAAGCCATTCCAGTGTAGAATTTGTATGCGTAATTTTTCTgtggcccacagc
ctgaagagaCATCTGAGGACCCACACCGGAGAGAAGCCTTTTCAATGCAGAATTTGTATG
CGTAATTTTTCTgaccccagcaacctgagaaggCATCTGAAGACCCACACCGGAGAAAAG
GGATCC
> ZF43.8(CO)
GCTAGCCCCAGAGTGAGAACCGGTTCTAAGACACCTCCCCACGAGAGGCCTTTTCAGTGT
AGAATTTGTATGCGTAATTTTTCTaggcaggacagactggacaggCACACAAGGACTCAC
ACTGGTGAAAAGCCCTTCCAATGTAGAATTTGTATGCGCAATTTTTCTcagaaggagcac
ctggccggcCATCTGAGGACCCACACAGGCGAAAAGCCTTTCCAGTGCAGAATTTGTATG
AGAAATTTTTCTaggagagacaacctgaacagaCATCTGAAGACACATACAGGTGAAAAA
GGATCC
> TEVp(FL)(CO)
ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT
TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC
TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA
AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA
ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT
TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT
TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC
ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG
TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT
AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG
ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC
TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG
GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC
> ZF21_16N-CfaN-CfaC-ZF21_16C(CO)
ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG
ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC
TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA
TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA
GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG
ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG
TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG
ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT
AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG
CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA
AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA
TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG
AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC
2018 team Fudan abstract

Abstract

Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.