Difference between revisions of "Team:Fudan/Primers"
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| + | <title>2018 iGEM Team:Fudan - Primers</title> | ||
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
</ul> | </ul> | ||
<ul id="dropdown2" class="dropdown-content"> | <ul id="dropdown2" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
<ul id="dropdown3" class="dropdown-content"> | <ul id="dropdown3" class="dropdown-content"> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
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| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<div class="collapsible-body"> | <div class="collapsible-body"> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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</div> | </div> | ||
<div class="col s12 m6 valign-wrapper hide-on-med-and-up"> | <div class="col s12 m6 valign-wrapper hide-on-med-and-up"> | ||
| − | <span> | + | <span>Here we present a library of the vast collection of primers we have designed</span> |
</div> | </div> | ||
</div> | </div> | ||
<div id="figureBannerTitle" class="hide-on-small-only"> | <div id="figureBannerTitle" class="hide-on-small-only"> | ||
<h1>Primers used</h1> | <h1>Primers used</h1> | ||
| − | <p><span> | + | <p><span>Here we present a library of the vast collection of primers we have designed</span></p> |
</div> | </div> | ||
<div class="hide-on-small-only"> | <div class="hide-on-small-only"> | ||
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<div class="section container"> | <div class="section container"> | ||
<h2>Primers</h2> | <h2>Primers</h2> | ||
| − | <div class="tableHolder" style="overflow | + | <div class="tableHolder" style="overflow:hidden"> |
| − | <table | + | <table> |
<tr><th>#</th><th>Oligo Name</th><th>DNA Sequence (5 to 3)</th></tr> | <tr><th>#</th><th>Oligo Name</th><th>DNA Sequence (5 to 3)</th></tr> | ||
<tr><td>O1</td><td>pLL7.1-F(S)</td><td>GGTCTATATAAGCAGAGCTGG</td></tr> | <tr><td>O1</td><td>pLL7.1-F(S)</td><td>GGTCTATATAAGCAGAGCTGG</td></tr> | ||
| Line 301: | Line 303: | ||
<tr><td>O26</td><td>mcherry-F(G_mNC)</td><td>TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr> | <tr><td>O26</td><td>mcherry-F(G_mNC)</td><td>TGTCCCGCAAGCGCCGGCGGATGGTGAGCAAGGGCGAG</td></tr> | ||
<tr><td>O27</td><td>mcherry-R(HindIII G_pML2)</td><td>AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG</td></tr> | <tr><td>O27</td><td>mcherry-R(HindIII G_pML2)</td><td>AAGTTATATTAAGGGTTCCAAGCTTTTACTTGTACAGCTCGTCCATG</td></tr> | ||
| − | <tr><td>O28</td><td>rev_41BB_CD3z9</td><td>序列缺失</td></tr> | + | <!-- tr><td>O28</td><td>rev_41BB_CD3z9</td><td>序列缺失</td></tr --> |
<tr><td>O29</td><td>hNotch-4168</td><td>CCCTGCCTGGGCGGCAAC</td></tr> | <tr><td>O29</td><td>hNotch-4168</td><td>CCCTGCCTGGGCGGCAAC</td></tr> | ||
<tr><td>O30</td><td>5286-hNotch</td><td>CCGCCGGCGCTTGCGGG</td></tr> | <tr><td>O30</td><td>5286-hNotch</td><td>CCGCCGGCGCTTGCGGG</td></tr> | ||
| Line 371: | Line 373: | ||
<tr><td>O99</td><td>1* ZF 54-8 & 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr> | <tr><td>O99</td><td>1* ZF 54-8 & 43-8 operator</td><td>GAATTCGCGGCCGCTTCTAG a TGG GTG GCA t a GAG TGA GGA c TACTAGTAGCGGCCGCTGCAG</td></tr> | ||
<tr><td>O100</td><td>2* ZFHD1</td><td>GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG</td></tr> | <tr><td>O100</td><td>2* ZFHD1</td><td>GAATTCGCGGCCGCTTCTAG TAATGATGGGCG TA TAATGATGGGCGTATAATGATGGGCGTATAATGATGGGCG TA TACTAG</td></tr> | ||
| − | <tr><td>O101</td><td>1*PIR</td><td>GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG</td></tr> | + | <tr><td>O101</td><td>1*PIR</td><td style="word-break: break-all">GAATTCGCGGCCGCTTCTAGGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACGAAATAGCGCTGTACAGCGTATGGGAATCTCTTGTACGGTGTACGAGTATCTTCCCGTACACCGTACTACTAGTAGCGGCCGCTGCAG</td></tr> |
<tr><td>O102</td><td>pCMV-F(S) </td><td>cgtgtacggtgggaggtctatataag</td></tr> | <tr><td>O102</td><td>pCMV-F(S) </td><td>cgtgtacggtgggaggtctatataag</td></tr> | ||
<tr><td>O103</td><td>pML2-R(S) </td><td>acgagattccagcaggtcg</td></tr> | <tr><td>O103</td><td>pML2-R(S) </td><td>acgagattccagcaggtcg</td></tr> | ||
| Line 605: | Line 607: | ||
<tr><td>O341</td><td>prefix-CD47tm(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr> | <tr><td>O341</td><td>prefix-CD47tm(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr> | ||
<tr><td>O342</td><td>CD47tm(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC</td></tr> | <tr><td>O342</td><td>CD47tm(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTACGGACCGGGATTTTCTTCC</td></tr> | ||
| − | <tr><td>O343</td><td>prefix-hYP9 | + | <tr><td>O343</td><td>prefix-hYP9 1scFv(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagc</td></tr> |
| − | <tr><td>O344</td><td>hYP9 | + | <tr><td>O344</td><td>hYP9 1scFv(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAACCTGGGACCCCCC</td></tr> |
<tr><td>O345</td><td>prefix-LaG 16-2(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr> | <tr><td>O345</td><td>prefix-LaG 16-2(CO)-F</td><td>GCAGCAGAATTCGCGGCCGCTTCTAGAGtgaaccgtcagatccgctagC</td></tr> | ||
<tr><td>O346</td><td>LaG 16-2(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC</td></tr> | <tr><td>O346</td><td>LaG 16-2(CO)-R-suffix</td><td>GCAGCACTGCAGCGGCCGCTACTAGTAGGGTTGCTACCGACGC</td></tr> | ||
| Line 647: | Line 649: | ||
<tr><td>O399</td><td>BS-R </td><td>TCTGCAGCGGCCGC</td></tr> | <tr><td>O399</td><td>BS-R </td><td>TCTGCAGCGGCCGC</td></tr> | ||
<tr><td>O400</td><td>pSB1A3-C(3-BP-BamHI) </td><td>GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg</td></tr> | <tr><td>O400</td><td>pSB1A3-C(3-BP-BamHI) </td><td>GCActctagaagcggccgcgaattcGGATCCgaaatcatccttagcgaaagctaagg</td></tr> | ||
| − | <tr><td>O401</td><td>pSB1A3-C(3-BS-HindIII | + | <tr><td>O401</td><td>pSB1A3-C(3-BS-HindIII C)</td><td>GCAtactagtagcggccgctgcagAAGCTTtccggcaaaaaagggcaagg</td></tr> |
<tr><td>O404</td><td>mNCe-F(M) </td><td>CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG</td></tr> | <tr><td>O404</td><td>mNCe-F(M) </td><td>CCTTGTGTTGGtAGTAATCCATGCTACAATCAAGGGACCTGCG</td></tr> | ||
<tr><td>O405</td><td>mNCe-F </td><td>CCTTGTGTTGGtAGTAATCCATGCTAC</td></tr> | <tr><td>O405</td><td>mNCe-F </td><td>CCTTGTGTTGGtAGTAATCCATGCTAC</td></tr> | ||
| Line 1,275: | Line 1,277: | ||
<!--Abstract on content page--> | <!--Abstract on content page--> | ||
<div id="abstractContent" class="z-depth-2"> | <div id="abstractContent" class="z-depth-2"> | ||
| − | <a href="#!"><img src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> | + | <a href="#!"><img alt="2018 team Fudan abstract" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a> |
<div class="container"> | <div class="container"> | ||
<h2 style="margin: 0;padding: 10px 0;">Abstract</h2> | <h2 style="margin: 0;padding: 10px 0;">Abstract</h2> | ||
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<div class="row"> | <div class="row"> | ||
<div id="sponsor" class="col m3 s12 row"> | <div id="sponsor" class="col m3 s12 row"> | ||
| − | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> | + | <a href="https://2018.igem.org/Team:Fudan" target="_blank"><img alt="2018 Team:Fudan logo white" class="col s3 m6 l3" style="position:relative; padding: 0 0.3rem; margin:-0.15rem 0; left: -0.45rem;" src="https://static.igem.org/mediawiki/2018/7/73/T--Fudan--teamLogoWhite.png"> |
</a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png"> | ||
</a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png"> | ||
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<span>Project</span> | <span>Project</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Demonstrate">Demonstration</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Antigen_Receptors">Antigen, Receptors</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Results">Transmembrane logic</a></li> |
<li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | <li><a href="https://2017.igem.org/Team:Fudan">2017.iGEM</a></li> | ||
</ul> | </ul> | ||
| Line 1,334: | Line 1,336: | ||
<span>Dry lab</span> | <span>Dry lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#ribo">Addon: ribo</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#TALE">Addon: TALE</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Addon#T2">Addon: T2</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#Transcriptional_Amplifer">Model: transcriptional amplifer</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Model#NotchLigandKinetics">Model: Notch-ligand kinetics</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Software">Software</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<span>Wet lab</span> | <span>Wet lab</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/InterLab">iGEM interLab</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Notebook">Our notebook</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Primers">Primers used</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Protocols">Protocols</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Safety">Safety</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<span>Toolbox</span> | <span>Toolbox</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Basic_Parts">Basic parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Composite_Parts">Composite parts</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Optimization">Optimization</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Parts_Collection">Parts collection</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Improve">Parts improvement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Measurement">Quantification</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<span>Outreach</span> | <span>Outreach</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Bio-Art">Bio-Art display</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Collaborations">Collaborations</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Design_Intention">Design intention</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Human_Practices">Human practices</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Public_Engagement">Public engagement</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
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<span>Team</span> | <span>Team</span> | ||
<ul> | <ul> | ||
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Acknowledgement">Acknowledgement</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Attributions">Attributions</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Heritage">Heritage</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Team">Members</a></li> |
| − | <li><a href=" | + | <li><a href="/Team:Fudan/Sponsors">Sponsors</a></li> |
</ul> | </ul> | ||
</div> | </div> | ||
| Line 1,391: | Line 1,393: | ||
<div class="footer-copyright"> | <div class="footer-copyright"> | ||
<div class="container"> | <div class="container"> | ||
| − | + | <div class="contactUS row"> | |
| − | + | <div class="col s12 m6 l4"><i class="fa fa-location-arrow"></i> Life Sci Bldg E301, 2005 Songhu Rd, Shanghai | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-fax"></i> +86-21-31246727 | |
| − | + | </div><div class="col s12 m6 l2"><i class="fa fa-envelope-o"></i> igem@fudan.edu.cn | |
| − | + | </div><div class="col s12 m6 l4"><i class="fa fa-twitter"></i> <i class="fa fa-wechat"></i> Fudan_iGEM | |
| − | + | </div> | |
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</div> | </div> | ||
</footer> | </footer> | ||
Latest revision as of 15:20, 7 November 2018
- Addon: ribo
- Addon: TALE
- Addon: T2
- Model: transcriptional amplifer
- Model: Notch-ligand kinetics
- Software
Primers used
Primers
gBlocks
> alphaCD19(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCTACCCCTATGATGTACCAGAT TACGCGGATATACAAATGACCCAGACTACGTCCAGCCTTTCCGCCTCCCTGGGTGATCGC GTGACAATCAGTTGTAGGGCTAGTCAGGACATAAGTAAATACCTCAACTGGTATCAACAG AAGCCTGACGGAACAGTTAAACTTCTTATCTATCATACAAGTAGGCTTCATTCAGGCGTT CCATCCAGATTTAGTGGATCTGGGAGCGGCACCGACTACTCACTCACGATTAGTAATCTG GAACAAGAGGACATCGCCACTTATTTTTGCCAGCAGGGTAACACTCTTCCCTATACATTC GGCGGCGGTACGAAACTCGAAATTACAGGGGGGGGTGGCAGTGGTGGCGGAGGTTCCGGT GGGGGTGGTTCAGAAGTAAAACTTCAGGAATCTGGTCCAGGGCTGGTTGCACCCAGCCAA TCCCTTTCCGTCACGTGCACCGTATCCGGGGTCTCCCTCCCAGACTACGGCGTGAGTTGG ATACGACAGCCGCCGCGCAAGGGGCTTGAGTGGCTGGGTGTCATTTGGGGATCTGAAACA ACGTATTATAATTCCGCTTTGAAATCCCGCCTCACTATTATCAAGGACAATTCTAAGAGC CAGGTCTTTCTGAAGATGAACAGCCTGCAAACGGATGACACAGCCATCTACTATTGTGCA AAGCACTATTACTATGGTGGTTCATACGCTATGGATTACTGGGGACAAGGGACATCCGTA ACCGTTTCATCACCCTGCGTCGGTAGCAACCC
> tTAA(CO) TGTCACGAAAGCGCCGACGGATGAGTCGATTGGATAAATCTAAGGTAATTAACAGCGCAC TGGAGCTTCTGAACGAAGTAGGTATCGAGGGGCTCACCACACGAAAATTGGCACAGAAGC TCGGAGTGGAGCAGCCGACTTTGTACTGGCACGTTAAGAATAAGAGGGCTTTGCTTGACG CGCTGGCCATTGAGATGCTCGACCGGCATCACACTCACTTCTGCCCGCTTGAAGGCGAGA GTTGGCAAGATTTCCTTAGGAACAATGCAAAGAGCTTTCGATGCGCCCTGCTTAGCCATC GGGACGGCGCAAAGGTTCATCTTGGGACTCGGCCTACTGAGAAGCAATATGAAACCCTGG AAAACCAGCTTGCTTTCCTTTGCCAACAGGGGTTCTCACTGGAGAATGCCCTGTACGCAC TCTCCGCGGTCGGCCATTTTACTCTCGGGTGTGTTTTGGAAGATCAGGAACACCAGGTAG CGAAAGAGGAGCGAGAAACGCCTACAACTGATTCCATGCCACCGTTGCTGAGACAAGCTA TAGAGTTGTTCGACCACCAGGGTGCTGAACCGGCGTTTCTCTTTGGTCTGGAACTCATTA TCTGCGGCCTTGAAAAGCAGCTTAAGTGCGAGAGTGGCGGTCCCGCAGATGCACTTGATG ACTTTGATTTGGATATGTTGCCCGCGGACGCTCTTGATGACTTCGATCTGGATATGCTGC CGGCTGATGCCCTCGACGATCTTGACCTGGATATGCTCCCAGGATGAaagcttggaaccc ttaatataacttcgtat
> TetOn3G(CO) GGAGGTgaattcGCCACCATGAGCCGACTGGACAAGAGCAAGGTAATCAATTCAGCTTTG GAGCTTCTGAACGGCGTGGGAATCGAGGGTCTCACCACCAGAAAGCTCGCGCAAAAATTG GGGGTGGAACAACCGACTCTCTATTGGCATGTTAAGAACAAGAGGGCCCTCCTTGACGCA CTCCCCATCGAAATGCTGGATCGACATCACACACATAGTTGTCCGCTGGAGGGGGAGTCT TGGCAAGATTTCCTCAGGAATAACGCGAAGAGTTATCGGTGCGCTCTCCTTAGTCATCGC GATGGAGCGAAAGTGCATCTGGGGACAAGACCGACGGAAAAACAATATGAGACGTTGGAG AACCAACTGGCATTCTTGTGCCAACAGGGGTTCAGCCTTGAAAATGCTCTTTACGCCTTG AGCGCTGTTGGGCACTTCACCTTGGGCTGTGTACTGGAGGAGCAGGAGCACCAGGTCGCA AAGGAGGAGCGCGAAACGCCCACCACAGATAGTATGCCACCCCTGTTGAAGCAGGCTATC GAGCTTTTTGATCGGCAGGGTGCTGAGCCTGCCTTTTTGTTCGGCCTTGAGCTTATAATT TGCGGCCTGGAAAAACAACTCAAGTGCGAAAGCGGTGGTCCCACAGACGCATTGGACGAT TTTGATCTTGACATGCTGCCAGCCGATGCTTTGGACGATTTTGACCTGGATATGTTGCCG GCAGATGCCCTCGACGACTTTGATCTTGATATGTTGCCGGGCTAAggatccGGAGGT
> mN1ce(CO) CCCTGCGTCGGTAGCAACCCCTGCTACAATCAAGGGACCTGCGAGCCAACGTCAGAAAAT CCCTTTTATAGATGCCTGTGCCCGGCAAAGTTCAACGGACTGCTGTGCCACATACTCGAC TACAGTTTTACTGGGGGGGCGGGCCGGGATATACCCCCGCCTCAGATAGAAGAGGCATGC GAACTGCCGGAATGCCAGGTCGATGCGGGCAATAAAGTCTGCAACCTCCAATGCAATAAT CACGCCTGCGGTTGGGACGGGGGTGACTGCTCACTTAACTTCAATGATCCGTGGAAGAAC TGCACCCAGTCCCTCCAATGTTGGAAATATTTTTCCGATGGTCATTGTGATAGCCAGTGC AATTCCGCAGGATGTTTGTTCGATGGGTTTGACTGCCAACTGACAGAGGGTCAGTGTAAT CCTCTTTACGACCAATACTGCAAGGATCATTTCAGTGATGGACATTGTGACCAAGGTTGT AACAGTGCGGAGTGCGAATGGGATGGGTTGGATTGTGCTGAGCATGTTCCTGAGCGCCTC GCAGCGGGAACTTTGGTCCTCGTTGTCCTCCTTCCGCCTGACCAGCTCAGAAATAATAGC TTTCACTTTCTTAGAGAACTCTCTCATGTGTTGCATACCAACGTAGTCTTTAAGCGCGAC GCTCAAGGACAGCAAATGATCTTCCCGTACTATGGGCACGAAGAAGAGCTTCGGAAACAC CCGATAAAAAGGAGTACGGTTGGTTGGGCGACGAGTAGTCTTTTGCCGGGCACGAGTGGT GGCAGACAACGGCGGGAACTCGATCCGATGGACATACGGGGGTCCATAGTGTATCTGGAG ATTGACAACCGACAGTGTGTTCAAAGTTCAAGCCAGTGTTTTCAATCAGCCACTGATGTA GCTGCGTTTCTCGGAGCGTTGGCCTCTCTTGGGAGCTTGAATATTCCGTATAAAATCGAA GCTGTAAAAAGCGAACCTGTCGAACCGCCATTGCCAAGCCAGTTGCACCTCATGTACGTG GCCGCTGCTGCGTTCGTACTCTTGTTCTTCGTAGGCTGCGGTGTTTTGCTGTCACGAAAG CGCCGACGG
> hN1c(CO) CCCTGTCTTGGAGGGAATCCGTGTTACAACCAAGGGACATGTGAACCCACGAGCGAGTCT CCTTTTTACAGGTGTCTCTGTCCAGCAAAATTTAATGGATTGCTTTGCCACATTCTGGAT TACTCATTTGGTGGGGGAGCGGGGCGGGACATACCGCCGCCCCTTATTGAAGAAGCATGC GAACTGCCCGAGTGCCAGGAGGATGCAGGTAACAAGGTATGCTCTCTTCAGTGCAATAAC CACGCTTGCGGGTGGGATGGTGGCGATTGCTCCTTGAATTTTAATGATCCTTGGAAAAAT TGCACCCAATCACTTCAGTGTTGGAAATACTTCTCAGATGGCCATTGCGATTCTCAGTGT AACAGCGCGGGATGCTTGTTTGACGGATTCGACTGTCAAAGGGCTGAGGGACAATGTAAT CCGCTCTACGATCAGTATTGTAAAGACCATTTCAGCGACGGCCACTGTGACCAAGGCTGC AATAGTGCCGAGTGTGAGTGGGATGGGTTGGATTGCGCGGAGCATGTTCCGGAGAGACTT GCGGCAGGTACGCTCGTGGTTGTTGTACTCATGCCGCCTGAGCAACTGAGGAATAGTAGC TTTCACTTCCTCCGGGAACTGAGCCGGGTTCTGCATACTAACGTTGTATTTAAGAGAGAT GCACATGGTCAGCAAATGATATTTCCGTACTACGGTCGGGAAGAGGAATTGCGGAAGCAC CCCATTAAGAGAGCTGCCGAGGGTTGGGCCGCTCCGGATGCCCTCCTTGGACAAGTAAAA GCCTCCCTCCTTCCCGGCGGATCTGAGGGTGGACGGAGACGCCGGGAACTCGATCCAATG GATGTAAGAGGGTCCATTGTGTACTTGGAGATTGATAATCGCCAGTGTGTGCAGGCGTCC TCACAATGTTTTCAAAGTGCGACCGATGTCGCAGCATTCTTGGGTGCCCTGGCATCATTG GGCAGCCTGAATATTCCATATAAGATCGAAGCGGTGCAATCCGAAACGGTGGAACCGCCT CCACCGGCACAGCTTCATTTCATGTATGTCGCAGCAGCAGCTTTCGTTTTGCTTTTCTTC GTAGGGTGTGGTGTTCTCCTTAGTCGAAAACGACGCCGG
> LaG17(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG GAGGACCTCATGGCCGATGTGCAGCTCGTCGAGTCAGGTGGGGGTCTCGTACAAGCTGGG GGGTCACTCAGACTGTCTTGCGCCGCCAGTGGCAGAACCATCTCTATGGCAGCTATGTCC TGGTTCAGACAGGCGCCAGGCAAGGAGAGGGAGTTTGTGGCGGGTATAAGTAGGTCCGCA GGAAGCGCGGTCCACGCCGATTCAGTCAAAGGTCGGTTCACGATAAGCCGGGATAACACT AAGAACACTCTTTATCTCCAGATGAACTCTCTGAAGGCCGAGGATACGGCTGTTTACTAT TGCGCTGTACGGACATCTGGGTTCTTCGGGAGCATACCGCGCACCGGAACAGCGTTTGAT TACTGGGGACAAGGTACACAGGTGACCGTTTCCCCCTGCGTCGGTAGCAACCC
> LaG16-2(CO) tgaaccgtcagatccgctagcgctaccggtGCCACCATGGCGCTGCCGGTCACGGCATTG CTCCTTCCGTTGGCCCTGCTCTTGCATGCCGCTAGACCCGAGCAAAAGTTGATCTCAGAG GAGGACCTCATGGCACAGGTCCAACTCGTCGAATCCGGAGGTCGCCTCGTGCAAGCTGGC GATTCACTTCGACTCTCTTGCGCGGCGTCAGGTCGGACCTTCAGCACGTCAGCTATGGCG TGGTTCCGCCAGGCGCCTGGGAGAGAGAGAGAGTTTGTAGCAGCCATCACATGGACTGTT GGAAATACTATATTGGGCGACTCCGTAAAAGGGCGCTTCACTATCTCACGAGATAGAGCG AAAAACACTGTAGATCTGCAAATGGACAATTTGGAGCCGGAGGATACTGCTGTGTATTAT TGCAGCGCTCGCTCTCGGGGGTACGTCCTCTCCGTACTCCGGAGCGTGGATTCATACGAC TACTGGGGACAGGGCACACAAGTAACGGTAAGCGGTGGGGGAGGGTCTGGTGGTGGAGGC TCAGGAGGCGGGGGATCTATGGCTCAGGTCCAGCTTGTAGAGAGCGGTGGTGGATTGGTG CAAGCAGGAGGGAGCCTCAGGCTTAGTTGTGCTGCCTCCGGTCGAACCTTCTCAAATTAT GCAATGGGTTGGTTTAGGCAAGCTCCTGGTAAAGAACGGGAGTTCGTGGCCGCAATTAGC TGGACGGGAGTTAGCACATATTACGCTGACTCTGTGAAAGGTCGGTTCACGATAAGTCGA GACAATGATAAGAATACGGTTTACGTCCAAATGAATAGCCTCATCCCGGAGGACACAGCT ATATATTATTGCGCTGCTGTACGGGCAAGATCCTTCTCAGATACTTATAGCAGGGTTAAC GAATACGATTATTGGGGGCAGGGAACGCAAGTCACTGTTCCCTGCGTCGGTAGCAACCC
> EGFPtm(CO) GGAAGAAAATCCCGGTCCGATGGCCCTTCCTGTAACGGCGCTCCTTCTCCCTCTTGCCC TCCTGCTCCATGCCGCACGACCAATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGG TGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCG AGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCA AGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCA GCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCT ACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGG TGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGG AGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATA TCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCG AGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCC CCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCA ACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCG GCATGGACGAGCTGTACAAGAATGCCGTTGGTCAAGACACGCAGGAAGTCATTGTTGTAC CCCACTCCCTGCCATTTAAGGTGGTAGTAATTTCAGCCATCCTTGCTCTCGTTGTGTTGA CAATTATTTCTCTGATAATTCTTATCATGCTCTGGCAAAAGAAACCTCGCTGAaagcttg gaacccttaatataacttcgtat
> CD19ECD(CO) TTGCTCCCACTGGCCTTGCTGCTTCATGCGGCACGGCCTTACCCCTATGATGTACCAGAT TACGCGGAAGAGCCTCTCGTCGTGAAAGTTGAAGAAGGTGACAATGCAGTTTTGCAATGT CTGAAAGGGACGTCTGACGGACCAACCCAACAGCTCACTTGGAGTCGCGAATCCCCGCTT AAGCCGTTCCTGAAGTTGTCTCTGGGGTTGCCGGGTCTTGGTATACACATGCGCCCGCTT GCGATATGGCTCTTCATCTTCAATGTCAGCCAACAGATGGGCGGGTTCTATCTCTGTCAA CCGGGACCGCCATCAGAGAAGGCGTGGCAACCTGGTTGGACGGTAAACGTGGAAGGTAGC GGGGAGTTGTTTCGCTGGAACGTTTCTGATCTCGGGGGCCTGGGTTGCGGTCTGAAAAAT CGCTCTAGCGAAGGGCCGAGTTCACCTTCCGGCAAGCTCATGTCCCCGAAACTCTACGTG TGGGCGAAGGATAGGCCAGAAATTTGGGAGGGAGAACCCCCCTGCCTGCCGCCCAGAGAT TCACTCAACCAGAGTCTCTCCCAAGATCTGACTATGGCCCCAGGAAGTACCTTGTGGCTT AGCTGCGGTGTACCGCCCGACTCCGTGAGCCGAGGGCCTCTCTCTTGGACACACGTTCAC CCTAAGGGGCCGAAATCATTGTTGAGTTTGGAACTCAAAGACGATAGGCCAGCAAGGGAT ATGTGGGTTATGGAGACCGGCCTTCTGCTCCCACGAGCGACCGCTCAGGATGCGGGCAAG TATTATTGCCACAGAGGAAATCTGACTATGTCTTTTCATCTGGAAATTACTGCGCGGCCA GTTTTGTGGCACTGGCTGTTGCGCACGGGGGGATGGAAGGAGCAGAAATTGATCTCTGA
> Gal4DBD(CO) atgaagctgctgagcagcatcgagcaggcctgtgacatctgccggctgaagaaactgaag tgcagcaaagaaaagcccaagtgcgccaagtgcctgaagaacaactgggagtgccggtac agccccaagaccaagagaagccccctgaccagagcccacctgaccgaggtggaaagccgg ctggaaagactggaacagctgtttctgctgatcttcccacgcgaggacctggacatgatc ctgaagatggacagcctccaggacatcaaggccctgctgaccggcctgttcgtgcaggac aacgtgaacaaggacgccgtgaccgacagactggccagcgtggaaaccgacatgcccctg accctgcggcagcacagaatcagcgccaccagcagcagcgaggaaagcagcaacaagggc cagcggcagctgacagtgtct
> ZF21.16(CO) GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCTCCTCACGAGAGGCCTTTTCAATGT AGAATTTGTATGCGTAATTTTTCTaggaacttcatcctccagagaCATACAAGGACTCAC ACAGGTGAAAAGCCTTTTCAGTGCAGAATTTGTATGCGCAATTTTTCTcagggcggtaac ctggtgagaCATCTGCGTACCCACACTGGTGAAAAGCCTTTCCAATGTAGAATTTGTATG CGAAATTTTTCTcagcaaaccggcctgaacgtgCATCTGAAGACACACACAGGCGAGAAG GGATCC
> ZF42.10(CO) GCTAGCCCTAGAGTGAGAACAGGTTCTAAGACACCACCACATGAAAGACCATTCCAATGT AGAATTTGTATGAGGAATTTTTCTaccggccagatcctggacagaCATACAAGGACCCAC ACCGGCGAAAAGCCATTCCAGTGTAGAATTTGTATGCGTAATTTTTCTgtggcccacagc ctgaagagaCATCTGAGGACCCACACCGGAGAGAAGCCTTTTCAATGCAGAATTTGTATG CGTAATTTTTCTgaccccagcaacctgagaaggCATCTGAAGACCCACACCGGAGAAAAG GGATCC
> ZF43.8(CO) GCTAGCCCCAGAGTGAGAACCGGTTCTAAGACACCTCCCCACGAGAGGCCTTTTCAGTGT AGAATTTGTATGCGTAATTTTTCTaggcaggacagactggacaggCACACAAGGACTCAC ACTGGTGAAAAGCCCTTCCAATGTAGAATTTGTATGCGCAATTTTTCTcagaaggagcac ctggccggcCATCTGAGGACCCACACAGGCGAAAAGCCTTTCCAGTGCAGAATTTGTATG AGAAATTTTTCTaggagagacaacctgaacagaCATCTGAAGACACATACAGGTGAAAAA GGATCC
> TEVp(FL)(CO) ATCACGTTAGGGCTAGTCGACGCCACCATGGGGGAGAGCCTGTTCAAAGGGCCCCGCGAT TATAACCCTATAAGCAGTACAATATGTCATCTGACTAACGAATCAGACGGACATACGACC TCCCTCTACGGGATAGGGTTCGGCCCCTTCATCATCACCAATAAGCACTTGTTTAGGCGA AATAACGGCACGCTTCTGGTACAGTCCCTGCACGGGGTCTTTAAGGTCAAGAATACGACA ACTCTCCAACAGCATCTTATTGATGGACGCGACATGATTATCATTAGAATGCCTAAAGAT TTTCCGCCGTTCCCACAAAAATTGAAGTTTAGAGAACCGCAAAGGGAAGAGCGCATTTGT TTGGTAACCACTAATTTTCAGACAAAGAGCATGTCCTCCATGGTTAGTGACACTTCATGC ACGTTTCCTTCTTCTGATGGTATATTTTGGAAACATTGGATACAGACGAAAGACGGACAG TGCGGGTCCCCTCTTGTCTCTACCAGGGACGGCTTTATCGTCGGCATACATTCAGCCAGT AATTTCACAAACACAAATAATTATTTTACTAGCGTGCCCAAAAATTTCATGGAGTTGCTG ACCAATCAAGAGGCACAGCAGTGGGTTTCTGGATGGAGACTCAACGCCGACAGCGTACTC TGGGGTGGACATAAGGTGTTCATGgtgAAACCCGAGGAACCCTTTCAGCCGGTGAAAGAG GCCACGCAACTGATGAACGAATTGGTCTATAGCCAGTGAAAGCTTCCCTCATTCAAGTAC
> ZF21_16N-CfaN-CfaC-ZF21_16C(CO) ATCACGTTAGGGCTAGTCGACGCCACCatgGCAAGTCCTAGGGTGCGGACCGGATCAAAG ACGCCGCCTCACGAACGACCATTTCAATGCAGGATCTGCATGCGGAACTTCTCCCGGAAC TTTATCCTCCAGCGGCATACACGGACCCATACCGGAGAAAAGCCCTTCCAGTGCCTCTCA TATGACACGGAAATTTTGACCGTCGAATATGGCTTTCTTCCTATCGGGAAGATCGTTGAA GAAAGAATTGAATGCACGGTCTATACCGTGGATAAGAATGGTTTTGTATACACTCAACCG ATAGCGCAGTGGCATAACAGGGGGGAGCAAGAAGTCTTTGAATACTGTTTGGAGGATGGG TCCATCATCAGGGCAACCAAGGACCATAAGTTCATGACCACTGATGGGCAGATGCTTCCG ATTGATGAAATTTTCGAAAGGGGCCTTGACCTGAAGCAGGTCGATGGGTTGCCAATGGTT AAGATCATATCACGGAAGAGCCTGGGGACACAGAATGTGTATGATATTGGAGTAGGCGAG CCGCACAACTTCCTCCTTAAAAATGGTCTCGTAGCTTCAAACTGTAGGATATGCATGCGA AACTTCTCCCAGGGGGGCAACTTGGTCAGGCACCTTCGGACGCATACCGGAGAGAAGCCA TTTCAGTGCAGGATTTGCATGCGAAATTTTTCTCAGCAAACCGGGTTGAATGTACATCTG AAAACGCATACAGGTGAGAAGGGCTCCTGAAAGCTTCCCTCATTCAAGTAC
Abstract
Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.