Difference between revisions of "Template:Munich/Phactory"

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{{Munich/PhactoryBootstrapCSS}}
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{{Munich/PhactoryCSS}}
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<html lang="en">
 
<html lang="en">
  
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     <meta charset="utf-8">
 
     <meta charset="utf-8">
 
     <meta name="viewport" content="width=device-width, initial-scale=1">
 
     <meta name="viewport" content="width=device-width, initial-scale=1">
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     <link rel="stylesheet" href="https://maxcdn.bootstrapcdn.com/bootstrap/4.1.0/css/bootstrap.min.css">
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     <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/4.7.0/css/font-awesome.min.css">
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     <link rel="stylesheet" href="css/T--Munich--CSS.css">
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     <script type="text/javascript" src="https://2018.igem.org/Template:Munich/PhactoryPopperJS?action=raw&amp;ctype=text/javascript"></script>
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    <script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
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     <script type="text/javascript" src="https://2018.igem.org/Template:Munich/PhactoryBootstrapJS?action=raw&amp;ctype=text/javascript"></script>
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     <script src="https://cdnjs.cloudflare.com/ajax/libs/popper.js/1.14.0/umd/popper.min.js"></script>
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     <script type="text/javascript" src="https://2018.igem.org/Template:Munich/PhactoryJS?action=raw&amp;ctype=text/javascript"></script>
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     <script src="https://maxcdn.bootstrapcdn.com/bootstrap/4.1.0/js/bootstrap.min.js"></script>
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     <script type="text/javascript" src="https://2018.igem.org/Template:Munich/PhactoryCalendarJS?action=raw&amp;ctype=text/javascript"></script>
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     <script style="text/javascrpit" src="js/controller.js"></script>
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</head>
 
</head>
  
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<body>
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<body style="">
  
  
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    <section id="nav-placeholder">
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  <section id="nav-placeholder">
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         <nav class="navbar navbar-expand-md navbar-dark fixed-top" style="background-color: transparent; z-index: 100;">
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         <nav class="shadow navbar navbar-expand-md navbar-dark fixed-top" style="background-color: rgba(30, 30, 30, 0.3); z-index: 100;">
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             <a class="navbar-brand" href="#">
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             <a class="navbar-brand" href="https://2018.igem.org/Team:Munich">
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    <img src="images/T--Munich--logo.png" alt="logo" style="width:40px;">
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                <img src="https://static.igem.org/mediawiki/2018/9/92/T--Munich--logo.png" alt="logo" style="width:40px;">
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    <h5 class="d-inline ml-3">Phactory</h5>
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                <h5 class="d-inline ml-3">Phactory</h5>
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  </a>
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            </a>
 
             <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#collapsibleNavbar">
 
             <button class="navbar-toggler" type="button" data-toggle="collapse" data-target="#collapsibleNavbar">
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    <span class="navbar-toggler-icon"></span>
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                <span class="navbar-toggler-icon"></span>
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  </button>
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            </button>
 
             <div class="collapse navbar-collapse justify-content-around" id="collapsibleNavbar">
 
             <div class="collapse navbar-collapse justify-content-around" id="collapsibleNavbar">
 
                 <ul class="navbar-nav">
 
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                     <li id="homeButton" class="nav-item active mx-3">
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                     <li id="homeButton" class="nav-item mx-3">
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                         <a class="nav-link" href="home.html">HOME</a>
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                         <a class="nav-link" href="https://2018.igem.org/Team:Munich">HOME</a>
 
                     </li>
 
                     </li>
 
                     <li class="nav-item dropdown mx-3">
 
                     <li class="nav-item dropdown mx-3">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
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        TEAM
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                             <a class="dropdown-item" href="UnderConstruction.html">Team Members</a>
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                             <a class="dropdown-item" href="UnderConstruction.html">Collaborations</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Collaborations">Collaborations</a>
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  <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Sponsors">Sponsors</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Attributions">Attributions</a>
 
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                     <li class="nav-item dropdown mx-3">
 
                     <li class="nav-item dropdown mx-3">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
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        PROJECT
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                            PROJECT
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      </a>
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                        </a>
 
                         <div class="dropdown-menu">
 
                         <div class="dropdown-menu">
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Description">Description</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Description">Description</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Design">Design</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Design">Design</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Experiments">Experienments</a>
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          <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Notebook">Notebook</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Protocols">Protocols</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/InterLab">InterLab</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/InterLab">InterLab</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Model">Model</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Model">Model</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Results">Results</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Results">Results</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Demonstrate">Demonstrate</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Demonstrate">Demonstrate</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Attributions">Attributions</a>
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                         </div>
 
                         </div>
 
                     </li>
 
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                     <li class="nav-item dropdown mx-3">
 
                     <li class="nav-item dropdown mx-3">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
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        PARTS
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      </a>
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                         <div class="dropdown-menu">
 
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Description">Description</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Parts">Parts Overview</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Design">Design</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Basic_Part">Basic Parts</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Experiments">Experienments</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Improve">Improved Parts</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/InterLab">InterLab</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Composite_Part">Composite Parts</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Model">Model</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Results">Results</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Demonstrate">Demonstrate</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Attributions">Attributions</a>
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                     <li class="nav-item dropdown mx-3">
 
                     <li class="nav-item dropdown mx-3">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
 
                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
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      HUMAN PRACTICES
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                            HUMAN PRACTICES
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      </a>
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                        </a>
 
                         <div class="dropdown-menu">
 
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Description">Description</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Human_Practices">Human Practices</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Design">Design</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Public_Engagement">Education & Engagement</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Experiments">Experienments</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Oracoli">Oracoli</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/InterLab">InterLab</a>
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<a class="dropdown-item" href="https://2018.igem.org/Team:Munich/European_Meetup">European Meetup</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Model">Model</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Results">Results</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Demonstrate">Demonstrate</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Attributions">Attributions</a>
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                         <a class="nav-link dropdown-toggle" href="#" id="navbardrop" data-toggle="dropdown">
 
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      AWARDS
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      </a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Description">Description</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Applied_Design">Applied Design</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Design">Design</a>
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                          <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Entrepreneurship">Entrepreneurship</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Experiments">Experienments</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Hardware">Hardware</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/InterLab">InterLab</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Measurement">Measurement</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Model">Model</a>
 
                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Model">Model</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Results">Results</a>
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                             <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Software">Software</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Demonstrate">Demonstrate</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Improve">Improve</a>
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                            <a class="dropdown-item" href="https://2018.igem.org/Team:Munich/Attributions">Attributions</a>
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                         <a class="nav-link" href="https://2018.igem.org/Team:Munich/Safety">JUDGING FORM</a>
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                         <a class="nav-link" href="https://igem.org/2018_Judging_Form?team=Munich" target="_blank">JUDGING FORM ⇗</a>
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                    <li class="nav-item mx-1 pt-2">
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                        <a href="https://www.facebook.com/Munich.iGEM/" class="fa fa-facebook" target="_blank"></a>
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                    </li>
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                    <li class="nav-item mx-1 pt-2">
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                        <a href="https://twitter.com/iGEM_Munich" class="fa fa-twitter" target="_blank"></a>
 
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<script>
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var url = window.location.href;
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$(function() {
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    $('.nav-link[href="'+url+'"]').addClass("active");
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    $('.dropdown-item[href="'+url+'"]').addClass("active");
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        <div class="text-center" style="position: relative;  height:80vh; width:100%; overflow: hidden;">
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            <video autoplay muted loop id="myVideo" style="min-height:80vh; min-width:100%;">
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  <source src="https://static.igem.org/mediawiki/2018/7/73/T--Munich--Phactory_Video.mp4" type="video/mp4">
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            <!--    <img class="img" src="images/Obeya%20Meeting.png" alt="Obeya Meeting" style="min-height: 100%; min-width:100%;"> -->
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            <div class="text-light" style="position: absolute; bottom:10vh; width: 100%; padding: 50px;">
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                <h1 class="display-1">Phactory</h1>
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                <h1 class="">Redefine Phage Production</h1>
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        <div>
 
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            <button class="tablink text-white btn" onclick="openCity('London', this, '#3B6ECD')" id="defaultOpen" style="font-size:1.5rem;"><img src="images/T--Munich--logo_DNA.png" class="mr-3" alt="logo">Weblab 1</button>
 
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            <button class="tablink text-white btn" onclick="openCity('Paris', this, '#D1525B')" style="font-size:1.5rem;"><img src="images/T--Munich--logo_SOUP.png" class="mr-3" alt="logo">Weblab 2</button>
 
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            <button class="tablink text-white btn" onclick="openCity('Tokyo', this, '#E2D763')" style="font-size:1.5rem;"><img src="images/T--Munich--logo_PHAGE.png" class="mr-3" alt="logo">Weblab 3</button>
 
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        <div id="London" class="tabcontent">
 
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            <h3 class="display-4">Create DNA</h3>
 
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            <p class="text-justify">One Problem of cell extract, is the stability of linear DNA. In escherichia coli, endogenous and exogenous linear DNA are differentiated by specific proteins, such as the recombinase complex RecBCD. It recognizes genomic double strand breaks via a specific sequence of 8 bases called Chi-site and prepares the DNA for homologous repair. However, foreign DNA lacks the Chi-site and is therefore degraded by RecBCD. For experiments in cell extract expensive and inefficient inhibitors are used. Since a knockout of RecBCD is lethal, we chose to modify the enzyme to enable selective depletion after cell lysis. Therefore, we came up with several depletion strategies:</p>
 
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        <div id="Paris" class="tabcontent">
 
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            <h3 class="display-4">Create Soup</h3>
 
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            <p class="text-justify">The financial advantage of producing home-made cell extract for in vitro protein expression is virtually reversed by the fact that it is tedious and time-consuming, with huge batch to batch variations of the resulting product. Therefore, our objective is to optimize the protocol for self-made cell extract from E. coli, by simplifying time consuming steps while eliminating the central sources of variation during the preparation process. The focus of our effort is to determine the influence of cultivation conditions on extract quality and optimizing the cell lysis for speed, protein yield and nal product quality. By implementing novel ways of extract characterization, we aim to gain a deeper understanding of cell-free systems.</p>
 
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        </div>
 
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        <div id="Tokyo" class="tabcontent">
 
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            <h3 class="display-4">Create Phages</h3>
 
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            <p class="text-justify">Generation of a universal platform for phage engineering: Our TX-TL system is superior to conventional bacterial based phage production in the following aspects: <br>  - Assembly of phages in vitro<br>  - Exchange of phage fibers in vitro<br>  - Establishment of homogeneous phage batches<br>  - Reduction of endotoxins within phage batches We will exemplarily engineer a non lytic fluorescent T7 phage, which targets a non primary E. coli strain.</p>
 
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Latest revision as of 08:55, 9 November 2018

Phactory