Difference between revisions of "Team:Manchester"

 
(234 intermediate revisions by 4 users not shown)
Line 1: Line 1:
−
{{Manchester}}
 
−
<html>
 
−
<head>
 
−
<title> The University of Man-cheese-ter</title>
 
−
<link rel="stylesheet" type="text/css" href="style.css"/>
 
−
</head>
 
  
−
<style>
+
{{Manchester/Navbar}}
−
body {
+
 
−
background-color: #EEEEEE;
+
<html lang="en">
−
font-family: Arial, Arial, Arial;
+
<title>Man-Cheester</title>
−
}
+
 
 +
<head>
 +
<meta http-equiv="X-UA-Compatible" content="IE=Edge">
 +
<meta name="description" content="iGEM WIKI">
 +
<meta name="keywords" content="Listeria Biosensor">
 +
<meta name="author" content="igemmanchester">
 +
<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
 +
 
 +
<!-- Main css -->
 +
<link rel="stylesheet" href="https://2018.igem.org/Team:Manchester/CSS">
 +
</head>
 +
<title> The University of Man-cheester</title>
 +
<body>
 +
 
 +
 
 +
 
 +
<h1> FIGHTING <i>LISTERIA</i> HYSTERIA</h1>
 +
 
 +
 
 +
</div>
 +
</div>
 +
 
 +
<center><img src="https://static.igem.org/mediawiki/2018/0/01/T--Manchester--Homepage1.jpeg"></center>
 +
 
 +
<div id="abstract">
 +
  <h2>ABSTRACT</h2>
 +
 
 +
<p><i><b>Listeria monocytogenes</b></i> is a Gram-positive, rod-shaped, food-borne bacterium, capable of causing the rare, but potentially fatal, disease <b>listeriosis</b>. <i>L. monocytogenes</i> can replicate at temperatures as low as 0°C, allowing it to survive in industrial and domestic refrigerators. <i>L. monocytogenes</i> is often found in soft cheeses, making many varieties of cheese unavailable to those who are immunosuppressed. <b>MAN-CHEESTER</b> aims to introduce the <i>agr</i> quorum sensing system from <i>L. monocytogenes</i> into bacteria used in the cheese making process. On detection of AIP, a key quorum sensing molecule of <i>L. monocytogenes</i>, a colour change will occur, causing the cheese to turn purple and alerting the consumer to its contamination. Our concept could be further developed to include other sources of <i>L. monocytogenes</i> contamination, such as meats, vegetables or kitchen surfaces, to prevent as many cases of listeriosis as possible.</p>
 +
 
 +
<br>
 +
 
 +
<center><button class="btn5"><a style="text-decoration:none; color: white"; width="auto" href="https://2018.igem.org/Team:Manchester/Description">Read more</a></button></center>
 +
 
 +
<br>
 +
<br>
 +
<center><button class="btn1"><a style="text-decoration:none; color: white" href="https://2018.igem.org/Team:Manchester/Experiments">Wet Lab</a></button> &nbsp; &nbsp; &nbsp; &nbsp;
 +
 
 +
 
 +
<button class="btn2"><a style="text-decoration:none; color: black" href="https://2018.igem.org/Team:Manchester/Model">Dry Lab</a></button>&nbsp; &nbsp; &nbsp; &nbsp;
 +
 
 +
 
 +
<button class="btn3"><a style="text-decoration:none; color: #62628c" href="https://2018.igem.org/Team:Manchester/Human_Practices">Human Practices</a></button> &nbsp; &nbsp; &nbsp; &nbsp;
 +
 
 +
<button class="btn4"><a style="text-decoration:none; color: white" href="https://2018.igem.org/Team:Manchester/Results">Achievements</a></button></center> &nbsp; &nbsp; &nbsp; &nbsp;
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</body>
 +
<style>
 +
 
 +
 
 +
 
  
 
a {
 
a {
−
text-decoration: none;
+
text-decoration: none;  
−
color: red;
+
 
}
 
}
−
+
 
−
#container {
+
h1 {
−
background-color: white;
+
border-bottom: none;
−
width: 800px;
+
text-align: center;
−
margin-left: auto;  
+
padding-top: 7%;
−
margin-right: auto;
+
color: #47476b;
 
}
 
}
  
−
#header {
+
h3 {
−
background-color: #66CCFF;
+
border-bottom: none;!important
−
color: black;
+
text-align: center;
−
text-align: center;  
+
color: #62628c;
 
}
 
}
  
−
#content{
+
h2{
−
padding: 10px;
+
text-align: center;
 +
color: #52527a;
 +
text-decoration: none;
 
}
 
}
  
−
#nav{
+
   
−
width: 180px;
+
−
float: left;
+
−
}
+
−
#nav ul {
+
−
list-style-type: none;
+
−
padding: 0;
+
−
+
−
.selected {
+
−
font-variant: bold;
+
 
}
 
}
  
 
#main {
 
#main {
−
width: 600px;
+
    text-align: center;
−
float: right;
+
 
}
 
}
  
−
#footer{
+
#mainpgpic {
−
clear: both;
+
  text-align: center;
−
padding: 10px;
+
−
background-color: #999999
+
−
color: white;
+
−
text-align: right;
+
 
}
 
}
 +
 +
p1 {
 +
 +
    text-align: justify;
 +
    text-justify: inter-word;
 +
    Font-size: 20px;
 +
    line-height: 2.6em;
 +
}
 +
 +
#abstract {
 +
  text-align: justify;
 +
  background-colour: white;
 +
  padding-right:10%;
 +
padding-left:10%;
 +
padding-bottom:10%;
 +
 +
}
 +
 +
.btn2 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/d/d1/T--Manchester--DryLabButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: black;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 300%;
 +
}
 +
 +
.btn2:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/9/96/T--Manchester--DryLabButton1.jpeg') no-repeat;
 +
    color: black;
 +
}
 +
 +
 +
.btn1 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/e/e9/T--Manchester--WetLabButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 300%;
 +
}
 +
 +
.btn1:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/a/a6/T--Manchester--WetLabButton1.jpeg') no-repeat;
 +
    color:white;
 +
}
 +
 +
 +
.btn3 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/2/29/T--Manchester--HPButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 250%;
 +
}
 +
 +
.btn3:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/9/91/T--Manchester--HPButton1.jpeg') no-repeat;
 +
    color:white;
 +
}
 +
 +
 +
.btn4 {
 +
height: 250px;
 +
width: 250px;
 +
background:transparent url('https://static.igem.org/mediawiki/2018/8/89/T--Manchester--TeamButton2.jpeg') no-repeat;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
alignment: center;
 +
transition-duration: 0.6s;
 +
padding: 10%
 +
/* IE 6/7 fallback */
 +
*text-indent: -9999px;
 +
font-size: 280%;
 +
}
 +
 +
.btn4:hover {
 +
    background: transparent url('https://static.igem.org/mediawiki/2018/5/59/T--Manchester--TeamButton1.jpeg') no-repeat;
 +
    color:white;
 +
}
 +
 +
 +
 +
.btn5 {
 +
height: 100px;
 +
width: 1100px;
 +
background:goldenrod;
 +
border-radius: 75px;
 +
border: 0;
 +
overflow: hidden;
 +
color: white;
 +
text-align: center;
 +
transition-duration: 0.6s;
 +
font-size: 300%;
 +
}
 +
 +
.btn5:hover {
 +
    background: #c6961d;
 +
    color:white;
 +
}
 +
 
</style>
 
</style>
−
<body>
+
 
−
+
</html>
−
<div id="container">
+
{{Manchester/Footer}}
−
<div id="header">
+
−
<h1>The University of Manchester</h1>
+
−
</div>
+
−
+
−
<div id="content">
+
−
<div id="nav">
+
−
<h3>Navigation</h3>
+
−
<ul>
+
−
<li><a class="selected" href="">Home</a></li>
+
−
<li>About the team</li>
+
−
<li>Our project</li>
+
−
<li>Lab Book</li>
+
−
<li>Contact</li>
+
−
</ul>
+
−
</div>
+
−
+
−
<div id="main">
+
−
<h2>Home Page</h2>
+
−
<img src="igempic/FINAL LOGO IGEM.PNG" width = "400" height="265"alt="iGEM 2018"/>
+
−
<p>What is iGEM?</p>
+
−
<p> Listeria is a genus of bacteria that, until 1992, contained 10 known species,[1][2] each containing two subspecies. As of 2014, another five species were identified.[3] Named after the British pioneer of sterile surgery Joseph Lister, the genus received its current name in 1940. Listeria specie</p>
+
−
<p>The first documented case of listeriosis was in 1924. In the late 1920s, two researchers independently identified L. monocytogenes from animal outbreaks. They proposed the genus Listerella in honor of surgeon and early antiseptic advocate Joseph Lister, but that name was already in use for a slime mold and a protozoan. Eventually, the genus Listeria was proposed and accepted. All species within the genus Listeria are Gram-positive, catalase-positive rods and do not produce endospores. The genus Listeria was classified in the family Corynebacteriaceae through the seventh edition of Bergey's Manual of Systematic Bacteriology. The 16S rRNA cataloging studies of Stackebrandt, et al. demonstrated that L. monocytogenes is a distinct taxon within the Lactobacillus-Bacillus branch of the bacterial phylogeny constructed by Woese. In 2004, the genus was placed in the newly created family Listeriaceae. The only other genus in the family is Brochothrix.[7]The genus Listeria currently contains 17 species: L. aquatica, L. booriae, L. cornellensis, L. fleischmannii, L. floridensis, L. grandensis, L. grayi, L. innocua, L. ivanovii, L. marthii, L. monocytogenes, L. newyorkensis, L. riparia, L. rocourtiae, L. seeligeri, L. weihenstephanensis, and L. welshimeri.[8] Listeria dinitrificans, previously thought to be part of the genus Listeria, was reclassified into the new genus Jonesia.[9] Under the microscope, Listeria species appear as small rods, which are sometimes arranged in short chains. In direct smears, they may be coccoid, so they can be mistaken for streptococci. Longer cells may resemble corynebacteria. Flagella are produced at room temperature, but not at 37 °C. Hemolytic activity on blood agar has been used as a marker to distinguish L. monocytogenes from other Listeria species, but it is not an absolutely definitive criterion. Further biochemical characterization may be necessary to distinguish between the different species of Listeria.</p>
+
−
</div>
+
−
+
−
<div id="footer">
+
−
+
−
</div>
+
−
</body>
+
−
<html>
+

Latest revision as of 14:35, 9 November 2018


HOME
PROJECT
Project Description
Results
Collaborations
Cheese Making
WET LAB
InterLab
Experiments
Protocols
Lab Book
Parts
DRY LAB
Promoter Tool
Experimental Model
HUMAN PRACTICES
Intergrated Human Practices
Outreach
Burt's Cheese
Rokiškio sūris
News Feed Analysis
Gmo's and Brexit
European Commission
TEAM

Man-Cheester The University of Man-cheester

FIGHTING LISTERIA HYSTERIA

ABSTRACT

Listeria monocytogenes is a Gram-positive, rod-shaped, food-borne bacterium, capable of causing the rare, but potentially fatal, disease listeriosis. L. monocytogenes can replicate at temperatures as low as 0°C, allowing it to survive in industrial and domestic refrigerators. L. monocytogenes is often found in soft cheeses, making many varieties of cheese unavailable to those who are immunosuppressed. MAN-CHEESTER aims to introduce the agr quorum sensing system from L. monocytogenes into bacteria used in the cheese making process. On detection of AIP, a key quorum sensing molecule of L. monocytogenes, a colour change will occur, causing the cheese to turn purple and alerting the consumer to its contamination. Our concept could be further developed to include other sources of L. monocytogenes contamination, such as meats, vegetables or kitchen surfaces, to prevent as many cases of listeriosis as possible.