Difference between revisions of "Team:Ecuador/Parts"

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{{Ecuador}}
 
{{Ecuador}}
 
<html>
 
<html>
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<head>
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<meta charset="utf-8">
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<div class="column full_size">
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#bigBox {
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<h1>Parts</h1>
+
  width:800px;
−
<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
+
  height: 300px;
−
<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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}
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padding-bottom: 20px;
  
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<div class="column full_size">
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<div class="highlight decoration_background">
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.ec--p{
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<h3>Note</h3>
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display: block;
−
<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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</head>
  
−
 
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<body>
−
<div class="column two_thirds_size">
+
<div class="ec--modeling--container">
−
<div class="highlight decoration_B_full">
+
<div class="ec--first--section">
−
 
+
<div class="ec--main--title">
−
<h3>Adding parts to the registry</h3>
+
<a name="MOD"  style="text-decoration: none;"><h1>PARTS</h1></a>
−
<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
+
</div>
−
 
+
<div class="ec--modeling--items">
−
<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
+
<h3>Index</h3>
−
<div class="button_link">
+
<ul class="circle">
−
<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
+
<a href="#INT" style="text-decoration: none;color: #000000"><li>Introduction</li></a>
−
ADD PARTS
+
<a href="#BAC" style="text-decoration: none;color: #000000"><li>Bone and cartilage regeneration</li></a>
−
</a>
+
<a href="#ELA" style="text-decoration: none;color: #000000"><li>Elasticity</li></a>
 +
<a href="#CELL" style="text-decoration: none;color: #000000"><li>Cellulose production </li></a>
 +
</ul>
 +
</div>
 +
 +
</div>
 +
 +
<div class="ec--nau--content">
 +
<a name="INT"></a>
 +
<div class="ec--h2">
 +
INTRODUCTION
 +
</div>
 +
 +
                <div class="ec--p">
 +
The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed).
 +
</div>
 +
<a name="BAC"></a>
 +
<div class="ec--h2">
 +
Fusion protein for bone and cartilage regeneration
 +
</div>
 +
 +
<div class="ec--h3">Coding parts</div>
 +
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804000" style="color:black;"><div class="ec--h4" style="color:black;">BBa_K2804000   
 +
 +
      </div></a>
 +
<strong>CBD cipA with a C-terminal linker</strong>
 +
<div class="ec--BMP--text">
 +
    <div class="ec--p">This part was design to try to improve the imperial college (2014) CBD cipA part  BBa_K1321014, because the CBD N-terminal is where the protein binds to the cellulose and the linker could interfer with this interaction.</div>
 +
  </div>
 +
  </div>
 +
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804006" style="color:black;"><div class="ec--h4">
 +
BBa_K2804006 
 +
 +
      </div>
 +
</a>
 +
<strong>Superfolder GFP with six-histidine tail</strong>
 +
<div class="ec--BMP--text">
 +
    <div class="ec--p">The adition of an histidine tail to part BBa_K1365020 was done because it allows the characterization of fusion proteins binding sites through atomic force microscopy, thanks to its affinity to the equipment nickel tip.</div>
 +
  </div>
 +
  </div>
 +
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804002" style="color:black;"><div class="ec--h4">BBa_K2804002         
 +
      </div></a>
 +
<strong>Bone morphogenetic protein 2 (BMP2)</strong>
 +
<div class="ec--BMP--text">
 +
    <div class="ec--p">
 +
    Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.<sup>1</sup>  
 +
   
 +
    </div>
 +
  </div>
 +
 +
<div class="ec--h3">Composite parts</div>
 +
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804001" style="color:black;"><div class="ec--h4">BBa_K2804001 
 +
      </div></a>
 +
<strong>CBD cipA fused to BMP2 under the control of LacI promoter</strong>
 +
<div class="ec--BMP--text">
 +
    <div class="ec--p">Therapeutic protein coupled to the CBD  whose expression is regulated by the presence of IPTG in the medium.</div>
 +
  </div>
 +
  </div>
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804004" style="color:black;"><div class="ec--h4">BBa_K2804004
 +
      </div></a>
 +
CBD cipA fused to sfGFP
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 +
</div>
 +
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804005" style="color:black;"><div class="ec--h4">BBa_K2804005
 +
      </div></a>
 +
CBD cipA fused to sfGFP under the control of LacI promoter
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 +
</div>
 +
<div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804014" style="color:black;"><div class="ec--h4">BBa_K2804014
 +
      </div></a>
 +
CBD cipA fused to sfGFP
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 +
</div>
 +
  <div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804015" style="color:black;"><div class="ec--h4">BBa_K2804015
 +
      </div></a>
 +
CBD cipA fused to BMP2
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 
</div>
 
</div>
−
 
+
<table border="0" cellspacing="0" cellpadding="0" width="703">
 +
  <tr>
 +
    <td width="175" nowrap valign="bottom"><p><strong>Name</strong></p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>Description</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p><strong>Type</strong></p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804000</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA with a C-terminal linker</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804001</p></td>
 +
    <td width="448" nowrap valign="bottom"><p>CBD cipA fused to BMP2 under the control of LacI    promoter</p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804002</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>Bone morphogenetic protein 2 (BMP2)</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804004</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to sfGFP</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804005</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to sfGFP under the control    of LacI promoter</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804006</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>Superfolder GFP with six-histidine tail</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804014</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to sfGFP</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  <tr>
 +
    <td width="175"><p>BBa_K2804015</p></td>
 +
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to BMP2</strong></p></td>
 +
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
 +
      </tr>
 +
  </table>
 +
<p>&nbsp;</p>
 +
 +
<a name="ELA"></a>
 +
<div class="ec--h2">
 +
Fusion protein to provide elasticity to the biomaterial
 +
</div>
 +
 +
<div class="ec--h3">Coding parts</div>
 +
 +
<div class="ec--BMP--container">
 +
  <a href="http://parts.igem.org/Part:BBa_K2804011" style="color:black;"><div class="ec--h4">BBa_K2804011   
 +
      </div></a>
 +
ELP V-150
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 +
</div>
 +
<div class="ec--BMP--container">
 +
  <a href="http://parts.igem.org/Part:BBa_K2804012" style="color:black;"><div class="ec--h4">BBa_K2804012 
 +
      </div></a>
 +
ELP V-150
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 +
</div>
 +
 +
<div class="ec--h3">Composite part</div>
 +
 +
  <div class="ec--BMP--container">
 +
<a href="http://parts.igem.org/Part:BBa_K2804013" style="color:black;"><div class="ec--h4">BBa_K2804013   
 +
      </div></a>
 +
CBD cipA fused to ELP_C5 under the control of LacI promoter
 +
<div class="ec--BMP--text">
 +
  <div class="ec--p"></div>
 +
</div>
 
</div>
 
</div>
−
</div>
+
<table border="0" cellspacing="0" cellpadding="0" width="703">
−
 
+
  <tr>
−
 
+
    <td width="175" nowrap valign="bottom"><p><strong>Name</strong></p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>Description</strong></p></td>
−
<div class="column third_size">
+
    <td width="80" nowrap valign="bottom"><p><strong>Type</strong></p></td>
−
<div class="highlight decoration_A_full">
+
      </tr>
−
<h3>Inspiration</h3>
+
  <tr>
−
<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
+
    <td width="175"><p>BBa_K2804011</p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>ELP V-150</strong></p></td>
−
<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
<ul>
+
      </tr>
−
<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
+
  <tr>
−
<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
+
    <td width="175"><p>BBa_K2804012</p></td>
−
<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
+
    <td width="448" nowrap valign="bottom"><p><strong>ELP_C5</strong></p></td>
−
</ul>
+
    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
−
</div>
+
      </tr>
−
</div>
+
  <tr>
−
 
+
    <td width="175"><p>BBa_K2804013</p></td>
−
 
+
    <td width="448" nowrap valign="bottom"><p><strong>CBD cipA fused to ELP_C5 under the control    of LacI promoter</strong></p></td>
−
<div class="clear extra_space"></div>
+
    <td width="80" nowrap valign="bottom"><p>Composite</p></td>
−
 
+
      </tr>
−
 
+
  </table>
−
 
+
<p>&nbsp;</p>
−
 
+
−
<div class="column full_size">
+
<a name="CELL"></a>
−
 
+
<div class="ec--h2">
−
<h3>What information do I need to start putting my parts on the Registry?</h3>
+
Cellulose production module
−
<p>The information needed to initially create a part on the Registry is:</p>
+
</div>
−
<ul>
+
−
<li>Part Name</li>
+
<div class="ec--h3">Coding parts</div>
−
<li>Part type</li>
+
−
<li>Creator</li>
+
<div class="ec--BMP--container">
−
<li>Sequence</li>
+
  <a href="http://parts.igem.org/Part:BBa_K2804008" style="color:black;">
−
<li>Short Description (60 characters on what the DNA does)</li>
+
  <div class="ec--h4">
−
<li>Long Description (Longer description of what the DNA does)</li>
+
  BBa_K2804008 
−
<li>Design considerations</li>
+
      </div>
−
</ul>
+
  </a>
−
 
+
CmcAx: Endo-beta-1,4-glucanase ezyme
−
<p>
+
<div class="ec--BMP--text">
−
We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
+
  <div class="ec--p"></div>
−
 
+
</div>
−
</div>
+
</div>
−
 
+
<div class="ec--BMP--container">
−
 
+
  <a href="http://parts.igem.org/Part:BBa_K2804009" style="color:black;"><div class="ec--h4">BBa_K2804009 
−
<div class="clear extra_space"></div>
+
      </div></a>
−
<div class="line_divider"></div>
+
CcpAx: Cellulose-complementing protein
−
<div class="clear extra_space"></div>
+
<div class="ec--BMP--text">
−
 
+
  <div class="ec--p"></div>
−
<div class="column full_size">
+
</div>
−
<h3>Part Table </h3>
+
</div>
−
 
+
  </div>
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
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Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx
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    <td width="175"><p>BBa_K2804008</p></td>
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    <td width="448" nowrap valign="bottom"><p><strong>CmcAx: Endo-beta-1,4-glucanase ezyme</strong></p></td>
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    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
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    <td width="175"><p>BBa_K2804009</p></td>
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    <td width="448" nowrap valign="bottom"><p><strong>CcpAx: Cellulose-complementing protein</strong></p></td>
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    <td width="80" nowrap valign="bottom"><p>Coding</p></td>
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    <td width="175"><p>BBa_K2804010</p></td>
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    <td width="448" nowrap valign="bottom"><p><strong>Operon member of bacterial cellulose    synthase complex: Cmcax and CcpAx</strong></p></td>
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<div class="ec--h4"><a name="RE"></a>REFERENCES</div>
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<div class="ec--p">Zhou,  N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. <a href="https://doi.org/10.1007/s00441-016-2403-0">https://doi.org/10.1007/s00441-016-2403-0</a></div>
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{{Ecuador/footer}}

Latest revision as of 02:32, 19 November 2018

iGEM ECUADOR

PARTS

Index

INTRODUCTION
The aim of our project is to obtain a novel bone and cartilage regeneration treatment. The path we take to carry out this was transforming E. coli to produce a fusion protein, conformed by a cellulose binding domain and a protein that induce chondrogenesis and osteogenesis, also a fusion protein conformed by CBD and an elastin like protein was design in order to obtain an elastic biomaterial. We also tried to produce cellulose in E. coli to have a better control in its production with a LacI repressed promoter. The parts are briefly described below (see the part page if more information is needed).
Fusion protein for bone and cartilage regeneration
Coding parts
BBa_K2804000
CBD cipA with a C-terminal linker
This part was design to try to improve the imperial college (2014) CBD cipA part BBa_K1321014, because the CBD N-terminal is where the protein binds to the cellulose and the linker could interfer with this interaction.
BBa_K2804006
Superfolder GFP with six-histidine tail
The adition of an histidine tail to part BBa_K1365020 was done because it allows the characterization of fusion proteins binding sites through atomic force microscopy, thanks to its affinity to the equipment nickel tip.
BBa_K2804002
Bone morphogenetic protein 2 (BMP2)
Bone morphogenetic protein 2 (BMP2), is a member of the transforming growth factor-β (TGF-β) super-family. It is involved in chondrogenesis and osteogenesis in vitro via the expression induction of Sox9, Runx2 and its downstream markers.1
Composite parts
BBa_K2804001
CBD cipA fused to BMP2 under the control of LacI promoter
Therapeutic protein coupled to the CBD whose expression is regulated by the presence of IPTG in the medium.
BBa_K2804004
CBD cipA fused to sfGFP
BBa_K2804005
CBD cipA fused to sfGFP under the control of LacI promoter
BBa_K2804014
CBD cipA fused to sfGFP
BBa_K2804015
CBD cipA fused to BMP2

Name

Description

Type

BBa_K2804000

CBD cipA with a C-terminal linker

Coding

BBa_K2804001

CBD cipA fused to BMP2 under the control of LacI promoter

Composite

BBa_K2804002

Bone morphogenetic protein 2 (BMP2)

Coding

BBa_K2804004

CBD cipA fused to sfGFP

Composite

BBa_K2804005

CBD cipA fused to sfGFP under the control of LacI promoter

Composite

BBa_K2804006

Superfolder GFP with six-histidine tail

Coding

BBa_K2804014

CBD cipA fused to sfGFP

Composite

BBa_K2804015

CBD cipA fused to BMP2

Composite

 

Fusion protein to provide elasticity to the biomaterial
Coding parts
BBa_K2804011
ELP V-150
BBa_K2804012
ELP V-150
Composite part
BBa_K2804013
CBD cipA fused to ELP_C5 under the control of LacI promoter

Name

Description

Type

BBa_K2804011

ELP V-150

Coding

BBa_K2804012

ELP_C5

Coding

BBa_K2804013

CBD cipA fused to ELP_C5 under the control of LacI promoter

Composite

 

Cellulose production module
Coding parts
BBa_K2804008
CmcAx: Endo-beta-1,4-glucanase ezyme
BBa_K2804009
CcpAx: Cellulose-complementing protein
Composite part
BBa_K2804010
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx

Name

Description

Type

BBa_K2804008

CmcAx: Endo-beta-1,4-glucanase ezyme

Coding

BBa_K2804009

CcpAx: Cellulose-complementing protein

Coding

BBa_K2804010

Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx

Composite
REFERENCES
Zhou, N., Li, Q., Lin, X. et al. Cell Tissue Res (2016) 366: 101. https://doi.org/10.1007/s00441-016-2403-0
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UNIVERSIDAD DE LAS FUERZAS ARMADAS ESPE.
Ecuador,Sangolquí, Av. General Rumiñahui s/n y Ambato