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| − | <div id="logo"><img src="https://static.igem.org/mediawiki/2018/f/f1/T--IISER-Kolkata--bactman_final.png"/></div> | + | <div id="logo "><a href="https://2018.igem.org/Team:IISER-Kolkata"><img src="https://static.igem.org/mediawiki/2018/ c/ cd/T--IISER-Kolkata-- bacman. jpg"/ ></a></div> |
| − | <div class="pagelink" id="homeMenu"><div><p>Home</p></div></div> | + | <div class="pagelink" id="homeMenu"><a href="https://2018.igem.org/Team:IISER-Kolkata">Home</a></div> |
| − | <div class="pagelink" id="aboutUsMenu"><div><p>Abstract</p></div></div> | + | <div class="pagelink" id="projectMenu"><a href="https://2018.igem.org/Team:IISER-Kolkata/Problem">Project</a></div> |
| − | <div class="pagelink" id="eventsMenu"><div><p>Description</p></div></div> | + | <div class="pagelink" id="labMenu"><a href="https://2018.igem.org/Team:IISER-Kolkata/Deletion">Lab</a></div> |
| − | <div class="pagelink" id="galleryMenu"><div><p>Details</p></div></div> | + | <div class="pagelink" id="achieveMenu"><a href="https://2018.igem.org/Team:IISER-Kolkata/Results">Achievements</a></div> |
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| − | <div id=" iiserk"><a href="https:// www. iiserkol. ac.in" target="_blank"><img src="https://static.igem.org/mediawiki/2018/ 4/ 4c/T--IISER-Kolkata-- iiserk.png"/></a ></div> | + | <a href="https:// 2018. igem. org/Competition/Results" target="_blank"><img id="goldmedal" src="https://static.igem.org/mediawiki/2018/ d/ d2/T--IISER-Kolkata-- gold.png"/></a> |
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| − | <div></div> | + | <h3>BacMan</h1> |
| − | <div id='main_title'><p style="font-family: blogger !important;font-size: 4vw ;">BactMan</p></div> | + | <p> A probiotic bacterial BATMAN to protect the citizens of Gotham from Arsenic Poisoning</p> |
| − | <div id="title_desc"><p style="font-family: sans-serif;"> A probiotic bacterial BATMAN to protect the citizens of Gotham from Arsenic Poisoning.</p>
| + | <div id="homeflex"> |
| − | <div id=" pagedown"> Down< /div > | + | <div class=" homebutton">< a href="https:// 2018.igem.org/ Team:IISER-Kolkata /Description"> <p>Project description</ p>< /a></div> |
| − | </section>
| + | <div class="homebutton"><a href="https://2018.igem.org/Team:IISER-Kolkata/Abstract"><p>Project abstract</p></a></div> |
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| − | <section id="aboutUs">
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| − | <img src="https://static.igem.org/mediawiki/2018/f/f1/T--IISER-Kolkata--bactman_final.png" alt='BactMan' style="height: 70vh;">
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| − | <p style="font-size: 1.2vw;font-family: sans-serif !important;">Though several remediation and decontamination techniques exist to remove Arsenic form water, yet Arsenic poisoning remains a severe ailment in highly affected regions such as West Bengal, India (the province of our Institute) and Bangladesh. <br><br> | + | |
| − | These cases of Arsenic toxicity arise due to heavy intake of the metal through food (rice, other cereals and vegetables) raised in Arsenic laden soil and water paddy. There are no preventive measures to these 'vectors' of the 'disease'. <br> <br>
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| − | We, Team IISER-Kolkata plan to design a probiotic bacteria that can efficiently intake and sequester Arsenic in the physicochemical conditions existing in the human gut. <br> <br>
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| − | Therefore, it will enable us to shield people highly sensitive to Arsenic from its poisoning using low cost but effective result yielding probiotic pill prescription. <br><br>
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| − | We aim to show the proof of concept of our project in Escherichia coli and later move on to probiotic strains such as Lactobacillus acidophilus.<br><br>
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| − | We also plan to design safe techniques to deliver the probiotic pill into the gastrointestinal tract where the bateria will compete with GI tract epithelium for Arsenic absorption.</p>
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| − | <section id="events"> | + | <section class="mainpage" id="sponsors"> |
| − | <div id='descript'> | + | <a href="http://www.dbtindia.nic.in/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/2/2c/T--IISER-Kolkata--dbt.jpg"/></a> |
| − | <p id="des_left" style="font-family: sans-serif;">
| + | <a href="http://www.iiserkol.ac.in/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/4/4c/T--IISER-Kolkata--iiserk.png"/></a> |
| − | <span style="font-size: 1.5vw;font-family: prime !important"><b>Description:</b></span><br><br>
| + | <a href="http://www.snapgene.com/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/5/5a/T--IISER-Kolkata--sg.png"/></a> |
| − | Starting with analyzing and surveying of severe challanges in our locality but also of global concern, we came across the issue of Arsenic contamination of groundwater as the project problem. We are in the process of performing ground surveys, social awareness visits and interviews of experts to obtain meaningful data on this problem. <br>
| + | <a href="https://www.genebridges.com/home" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/e/e9/T--IISER-Kolkata--gb.jpg"/></a> |
| − | However, upon preliminary examination it has come to our notice that though water filtration and decontamination plants exist to remove Arsenic from potable water, yet use of untreated water for irrigation of food crops (rice) and/or raising fishes etc. leads to inclusion of the heavy metal into the staple diet of the locals. Arsenic ingestion through food causes severe poisoning and several long term effects even leading to cancer. <br><br>
| + | <a href="https://www.neb.com/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/7/7e/T--IISER-Kolkata--neb.jpg"/></a> |
| − | Present purification methods do not cover this side of the problem leaving people open to the risk of Arsenic toxicity. Team iGEM 2018 IISER Kolkata therefore plans to put synthetic biology to use to combat Arsenic toxicity mainly caused by heavy metal laden ingested food.<br>
| + | <a href="https://eu.idtdna.com/pages" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/2/2e/T--IISER-Kolkata--idt.png"/></a> |
| − | Our aim is to design a probiotic Arsenic sequestering bacteria which will be administered as an inexpensive pill to colonize gut and eliminate the toxic heavy metal entering through diet (mostly food). <br>
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| − | We will first test the idea and establish proof of concept in the easy to work with gram negative E. coli species often called the lab rat of molecular biology and only then move on to working with gram positive Lactobacillus (probiotic species).<br><br>
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| − | <span style="font-size: 1.5vw;font-family: prime !important"><b>Significance:</b> </span><br><br>
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| − | 1. Novel prevention based approach as a long term solution to Arsenic Poisoning in severely affected regions of the world.<br>
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| − | 2. The problem is severe and relatively undealt with. It is of particular importance in the province of our Institute (West Bengal, India), but is also present in other parts of the globe.<br>
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| − | 3. Our approach is innovative, cheap and effective.<br>
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| − | </div>
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| | </section> | | </section> |
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| − | <section id="gallery"> | + | <section class="mainpage" id="contactUs"> |
| − | <div id='details'> | + | <div id="contactLeft"> |
| − | <p id='det_cont'> | + | <h4>Visit us Physically</h4> |
| − | <span style="font-size: 1.5vw;font-family: prime !important"><b>Details:</b></span><br><br>
| + | <p>Lab Number 216, Research Complex Building<br> |
| − | Arsenic is present mostly in its toxic inorganic +3 and +5 ionic forms in water and food that manifest into the poisoning symptoms when ingested. These As+3 and As+5 ions naturally permeate into the bacterial cell through its Phosphate transporters. Most bacteria including E. coli and Lactobacillus however contain ars Operon that confers Arsenic tolerance to it principally by exporting back to the exterior the unintentionally intaken Arsenic ions. <br>
| + | IISER Kolkata Campus<br> |
| − | This in built resistance mechanism of the bacterium is hampering to our plan as we intend to make the bacteria intake and sequester Arsenic within. Therfore, we will first try to delete arsA and arsB genes of the chromosomally located ars Operon using a gene deletion kit. <br>
| + | Near Harringhata Farms<br> |
| − | To sequester Arsenic within the bacteria, a Synthetic Phytochelatin under a strong T7 promoter will be used. However, to keep the entire circuit under the control of Arsenic exposure to the cell, the T7 RNA polymerase required for Phytochelatin gene transcription will itself be under arsR the promoter operator that is turned on by deinhibition of ars promoter by binding of As(III) and/or Ars(IV) to the ArsR (repressor). <br><br>
| + | Mohanpur, Dist: Nadia<br> |
| − | Along with phytochelatin, we have planned to include a gene encoding HMT (heavy metal tolerance protein) from S. pombe and another encoding AOX (Arsenic Oxidase) from Thermus thermophilus to make an Arsenic Responsive Operon. HMT is known to transport Arsenic bound Phytochelatin to the periplasm in gram negative bacteria. <br>
| + | West Bengal, India-741246</p> |
| − | This will help reduce the cytosolic concentration of arsenic as well as phytochelatin driving the equilibrium to cause more Arsenic intake and Phytochelatin production. AOX is an outer membrane protein in gram negative bacteria which converts external As(III) to As(V). This will help to reduce the more toxic As(III) present in the gut into a relatively docile As(V) form. <br>
| + | <h4>Visit us virtually</h4> |
| − | Finally, we plan to design an effecient probiotic pill delivery system so that the bacteria can be accurately targetted to the small intestine where majority of the Arsenic absorption occurs. Through out the course of the project we will incorporate the data obtained through "Human Practices exercise" thus integrating newly obtained information with ongoing lab work for a more realistic achievement of our goals.
| + | <a href="http://www.iiserkol.ac.in/" target="_blank">IISER Kolkata website</a> |
| − | </p>
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| | </div> | | </div> |
| − | </section>
| + | <div id="contactRight"> |
| − | | + | <h4>Write to us</h4> |
| − | <section id="ourTeam">
| + | <a href="mailto:psray@iiserkol.ac.in">Dr. Partho Sarothi Ray (Primary PI)</a><br> |
| − | <div id='team'>
| + | <a href="mailto:arnab.gupta@iiserkol.ac.in">Dr. Arnab Gupta (Secondary PI)</a><br> |
| − | <p id='team_left'>
| + | <a href="mailto:ppr16ms055@iiserkol.ac.in">Paresh Rana (Team Leader)</a><br> |
| − | <span style='font-size: 2vw;'><b>Dr. Partho Sarothi Ray</b></span><br><br>
| + | <a href="mailto:team.igem@iiserkol.ac.in">Team iGEM IISER-Kolkata</a><br> |
| − | <span style="display:inline-block; width: 1vw;"></span>Primary PI<br><br><br><br>
| + | <h4>Talk to us</h4> |
| − | | + | <p>Paresh Rana : +91-9078203201<br> |
| − | <span style='font-size: 2vw;'><b>Dr. Arnab Gupta</b></span><br><br>
| + | Animish Nilesh Vaze : +91-9273101997</p> |
| − | <span style="display:inline-block; width: 1vw;"></span>Secondary PI<br><br><br><br>
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| − | <span style='font-size: 2vw;'><b>Mr. Debabrato Sutradhar</b></span><br><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>Project Instructor
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| − | </p>
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| − | <p id='team_right'>
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| − | <span style='font-size: 1.2vw;'><b>Paresh Priyadarshan Rana</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Team Leader<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Animish Nilesh Vaze</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Skeptical Experimentalist<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Diptatanu Das</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Skilled Microbiologist <br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Pallab Datta</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Intelligent Artist<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Anmol Kumar</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Cheerful Professionalist<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Ankit Amrutkar</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Righteous Physicist<br><br><br>
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| − | </p> | + | |
| − | <p id='team_right2'>
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| − | <span style='font-size: 1.2vw;'><b>Hrishika Rai</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Starved Biologist<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Madhavi Andhari</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Confused Hardworker<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Souvik Ghosh</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Drunk Craftsman<br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Prajwal Padmanabha</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span><describe yourself><br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Pavan Vynatheya</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span><describe yourself><br><br><br>
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| − | <span style='font-size: 1.2vw;'><b>Rajalaxmi Saha</b></span><br>
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| − | <span style="display:inline-block; width: 1vw;"></span>The Ghost
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| − | </p>
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| | </div> | | </div> |
| − | </section>
| + | <div id="social"> |
| − | | + | <a class="links" id="socFacebook" href="https://www.facebook.com/iGEM.IISERK/" target="_blank">M</a> |
| − | <section id="sponsors">
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| + | <div id="footer"><p>Website created by Pavan Vynatheya and Prajwal Padmanabha</p></div> |
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