Difference between revisions of "Team:IISER-Kolkata"

 
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<div id="logo"><a href="https://2018.igem.org/Team:IISER-Kolkata"><img src="https://static.igem.org/mediawiki/2018/c/cd/T--IISER-Kolkata--bacman.jpg"/></a></div>
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<div class="pagelink" id="homeMenu"><div><p>Home</p></div></div>
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<div class="pagelink" id="homeMenu"><a href="https://2018.igem.org/Team:IISER-Kolkata">Home</a></div>
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<div class="pagelink" id="aboutUsMenu"><div><p>Abstract</p></div></div>
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<div class="pagelink" id="projectMenu"><a href="https://2018.igem.org/Team:IISER-Kolkata/Problem">Project</a></div>
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<div id="iiserk"><a href="https://www.iiserkol.ac.in" target="_blank"><img src="https://static.igem.org/mediawiki/2018/4/4c/T--IISER-Kolkata--iiserk.png"/></a></div>
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<h3>BacMan</h1>
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<div id='main_title'><p style="font-family: blogger !important;font-size: 4vw ;">BacMan</p></div>
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<p> A probiotic bacterial BATMAN to protect the citizens of Gotham from Arsenic Poisoning</p>
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<div id="title_desc"><p style="font-family: sans-serif;"> A probiotic bacterial BATMAN to protect the citizens of Gotham from Arsenic Poisoning.</p>
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<div class="homebutton"><a href="https://2018.igem.org/Team:IISER-Kolkata/Description"><p>Project description</p></a></div>
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<div class="homebutton"><a href="https://2018.igem.org/Team:IISER-Kolkata/Abstract"><p>Project abstract</p></a></div>
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<img src="https://static.igem.org/mediawiki/2018/f/f1/T--IISER-Kolkata--bactman_final.png" alt='BactMan' style="width:25vw;">
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<p style="font-size: 1.2vw;font-family: sans-serif !important;">Though several remediation and decontamination techniques exist to remove Arsenic form water, yet Arsenic poisoning remains a severe ailment in highly affected regions such as West Bengal, India (the province of our Institute) and Bangladesh. <br><br>
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These cases of Arsenic toxicity arise due to heavy intake of the metal through food (rice, other cereals and vegetables) raised in Arsenic laden soil and water paddy. There are no preventive measures to these 'vectors' of the 'disease'. <br> <br>
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We, Team IISER-Kolkata plan to design a probiotic bacteria that can efficiently intake and sequester Arsenic in the physicochemical conditions existing in the human gut. <br> <br>
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Therefore, it will enable us to shield people highly sensitive to Arsenic from its poisoning using low cost but effective result yielding probiotic pill prescription. <br><br>
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We aim to show the proof of concept of our project in Escherichia coli and later move on to probiotic strains such as Lactobacillus acidophilus.<br><br>
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We also plan to design safe techniques to deliver the probiotic pill into the gastrointestinal tract where the bateria will compete with GI tract epithelium for Arsenic absorption.</p>
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<a href="http://www.dbtindia.nic.in/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/2/2c/T--IISER-Kolkata--dbt.jpg"/></a>
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<a href="http://www.iiserkol.ac.in/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/4/4c/T--IISER-Kolkata--iiserk.png"/></a>
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<span style="font-size: 1.5vw;font-family: prime !important"><b>Description:</b></span><br><br>
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<a href="http://www.snapgene.com/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/5/5a/T--IISER-Kolkata--sg.png"/></a>
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Starting with analyzing and surveying of severe challanges in our locality but also of global concern, we came across the issue of Arsenic contamination of groundwater as the project problem. We are in the process of performing ground surveys, social awareness visits and interviews of experts to obtain meaningful data on this problem. <br>
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<a href="https://www.genebridges.com/home" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/e/e9/T--IISER-Kolkata--gb.jpg"/></a>
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However, upon preliminary examination it has come to our notice that though water filtration and decontamination plants exist to remove Arsenic from potable water, yet use of untreated water for irrigation of food crops (rice) and/or raising fishes etc. leads to inclusion of the heavy metal into the staple diet of the locals. Arsenic ingestion through food causes severe poisoning and several long term effects even leading to cancer. <br><br>
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<a href="https://www.neb.com/" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/7/7e/T--IISER-Kolkata--neb.jpg"/></a>
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Present purification methods do not cover this side of the problem leaving people open to the risk of Arsenic toxicity. Team iGEM 2018 IISER Kolkata therefore plans to put synthetic biology to use to combat Arsenic toxicity mainly caused by heavy metal laden ingested food.<br>
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<a href="https://eu.idtdna.com/pages" target="_blank"><img class="sponImg" src="https://static.igem.org/mediawiki/2018/2/2e/T--IISER-Kolkata--idt.png"/></a>
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Our aim is to design a probiotic Arsenic sequestering bacteria which will be administered as an inexpensive pill to colonize gut and eliminate the toxic heavy metal entering through diet (mostly food). <br>
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We will first test the idea and establish proof of concept in the easy to work with gram negative E. coli species often called the lab rat of molecular biology and only then move on to working with gram positive Lactobacillus (probiotic species).<br><br>
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<span style="font-size: 1.5vw;font-family: prime !important"><b>Significance:</b> </span><br><br>
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1. Novel prevention based approach as a long term solution to Arsenic Poisoning in severely affected regions of the world.<br>
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2. The problem is severe and relatively undealt with. It is of particular importance in the province of our Institute (West Bengal, India), but is also present in other parts of the globe.<br>
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3. Our approach is innovative, cheap and effective.<br>
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<h4>Visit us Physically</h4>
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<span style="font-size: 1.5vw;font-family: prime !important"><b>Details:</b></span><br><br>
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<p>Lab Number 216, Research Complex Building<br>
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Arsenic is present mostly in its toxic inorganic +3 and +5 ionic forms in water and food that manifest into the poisoning symptoms when ingested. These As+3 and As+5 ions naturally permeate into the bacterial cell through its Phosphate transporters. Most bacteria including E. coli and Lactobacillus however contain ars Operon that confers Arsenic tolerance to it principally by exporting back to the exterior the unintentionally intaken Arsenic ions. <br>
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IISER Kolkata Campus<br>
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This in built resistance mechanism of the bacterium is hampering to our plan as we intend to make the bacteria intake and sequester Arsenic within. Therfore, we will first try to delete arsA and arsB genes of the chromosomally located ars Operon using a gene deletion kit. <br>
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Near Harringhata Farms<br>
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To sequester Arsenic within the bacteria, a Synthetic Phytochelatin under a strong T7 promoter will be used. However, to keep the entire circuit under the control of Arsenic exposure to the cell, the T7 RNA polymerase required for Phytochelatin gene transcription will itself be under arsR the promoter operator that is turned on by deinhibition of ars promoter by binding of As(III) and/or Ars(IV) to the ArsR (repressor). <br><br>
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Mohanpur, Dist: Nadia<br>
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Along with phytochelatin, we have planned to include a gene encoding HMT (heavy metal tolerance protein) from S. pombe and another encoding AOX (Arsenic Oxidase) from Thermus thermophilus to make an Arsenic Responsive Operon. HMT is known to transport Arsenic bound Phytochelatin to the periplasm in gram negative bacteria. <br>
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West Bengal, India-741246</p>
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This will help reduce the cytosolic concentration of arsenic as well as phytochelatin driving the equilibrium to cause more Arsenic intake and Phytochelatin production. AOX is an outer membrane protein in gram negative bacteria which converts external As(III) to As(V). This will help to reduce the more toxic As(III) present in the gut into a relatively docile As(V) form. <br>
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<h4>Visit us virtually</h4>
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Finally, we plan to design an effecient probiotic pill delivery system so that the bacteria can be accurately targetted to the small intestine where majority of the Arsenic absorption occurs. Through out the course of the project we will incorporate the data obtained through "Human Practices exercise" thus integrating newly obtained information with ongoing lab work for a more realistic achievement of our goals.
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<a href="http://www.iiserkol.ac.in/" target="_blank">IISER Kolkata website</a>
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<h4>Write to us</h4>
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<a href="mailto:psray@iiserkol.ac.in">Dr. Partho Sarothi Ray (Primary PI)</a><br>
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<a href="mailto:arnab.gupta@iiserkol.ac.in">Dr. Arnab Gupta (Secondary PI)</a><br>
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<p id='team_left'>
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<a href="mailto:ppr16ms055@iiserkol.ac.in">Paresh Rana (Team Leader)</a><br>
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<span style='font-size: 2vw;'><b>Dr. Partho Sarothi Ray</b></span><br><br>
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<a href="mailto:team.igem@iiserkol.ac.in">Team iGEM IISER-Kolkata</a><br>
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<span class='ver_tab'></span>Primary PI<br><br><br><br>
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<h4>Talk to us</h4>
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<p>Paresh Rana : +91-9078203201<br>
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<span style='font-size: 2vw;'><b>Dr. Arnab Gupta</b></span><br><br>
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Animish Nilesh Vaze : +91-9273101997</p>
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<span class='ver_tab'></span>Secondary PI<br><br><br><br>
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<span style='font-size: 2vw;'><b>Mr. Debabrato Sutradhar</b></span><br><br>
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<span class='ver_tab'></span>Project Instructor
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<span style='font-size: 1.2vw;'><b>Paresh Priyadarshan Rana</b></span><br>
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<span class='ver_tab'></span>The Team Leader<br><br><br>
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<span style='font-size: 1.2vw;'><b>Animish Nilesh Vaze</b></span><br>
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<span class='ver_tab'></span>The Skeptical Experimentalist<br><br><br>
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<span style='font-size: 1.2vw;'><b>Diptatanu Das</b></span><br>
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<span class='ver_tab'></span>The Skilled Microbiologist <br><br><br>
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<span style='font-size: 1.2vw;'><b>Pallab Datta</b></span><br>
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<span class='ver_tab'></span>The Intelligent Artist<br><br><br>
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<span style='font-size: 1.2vw;'><b>Anmol Kumar</b></span><br>
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<span class='ver_tab'></span>The Cheerful Professionalist<br><br><br>
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<span style='font-size: 1.2vw;'><b>Ankit Amrutkar</b></span><br>
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<span class='ver_tab'></span>The Righteous Physicist<br><br><br>
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<span style='font-size: 1.2vw;'><b>Hrishika Rai</b></span><br>
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<span class='ver_tab'></span>The Starved Biologist<br><br><br>
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<span style='font-size: 1.2vw;'><b>Madhavi Andhari</b></span><br>
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<span class='ver_tab'></span>The Confused Hardworker<br><br><br>
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<span style='font-size: 1.2vw;'><b>Souvik Ghosh</b></span><br>
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<span class='ver_tab'></span>The Drunk Craftsman<br><br><br>
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<span style='font-size: 1.2vw;'><b>Prajwal Padmanabha</b></span><br>
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<span class='ver_tab'></span>&ltdescribe yourself&gt<br><br><br>
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<span style='font-size: 1.2vw;'><b>Pavan Vynatheya</b></span><br>
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<span class='ver_tab'></span>&ltdescribe yourself&gt<br><br><br>
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<span style='font-size: 1.2vw;'><b>Rajalaxmi Saha</b></span><br>
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<span class='ver_tab'></span>The Ghost
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<a class="links" id="socFacebook" href="https://www.facebook.com/iGEM.IISERK/" target="_blank">M</a>
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<a class="links" id="socTwitter" href="https://twitter.com/iGEM_IISERKol" target="_blank">N</a>
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<img id='dbt' src='https://static.igem.org/mediawiki/2018/2/2c/T--IISER-Kolkata--dbt.jpg' alt='Department of Biotechnology'>
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Department of Biotechnology
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<p id='dbt_des'>
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Selected and Awarded Funds through iBEC (pre-iGEM India level competition)
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<img id='gb' src='https://static.igem.org/mediawiki/2018/e/e9/T--IISER-Kolkata--gb.jpg' alt='Gene Bridges'>
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GeneBridges
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<p id='gb_des'>
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Company producing and distributing products and kits for use in Recombinant DNA technology
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<img id='idt' src="https://static.igem.org/mediawiki/2018/2/2e/T--IISER-Kolkata--idt.png" alt='Integrated DNA Technologies'>
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Integrated DNA Technologies
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<p id=idt_des>
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Official sponsor for all iGEM teams supplying customized synthesized gBlocks for upto 20kb genes
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</p>
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<img id='ik' src="https://static.igem.org/mediawiki/2018/4/4c/T--IISER-Kolkata--iiserk.png" alt='IISER Kolkata'>
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<p id='ik_text'>
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IISER Kolkata<br>
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Home Institute<br><br>
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Providing financial assistance to the team
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</p>
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<img id='neb' src="https://static.igem.org/mediawiki/2018/7/7e/T--IISER-Kolkata--neb.jpg" alt='New England Biolabs'>
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<p id='neb_text'>
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New England Biolabs<br>
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</p>
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<p id='neb_des'>
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Official sponsor for all iGEM teams supplying required Mol Bio Kits and chemicals
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</p>
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<img id='sg' src="https://static.igem.org/mediawiki/2018/5/5a/T--IISER-Kolkata--sg.png" alt='SnapGene'>
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SnapGene<br>
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<p id='sg_des'>
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Company producing and distributing softwares for Molecular Biology
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<p id='phys'>
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<b>Visit Us Physically:</b><br><br>
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Lab Number 216, Research Complex Building<br>
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IISER Kolkata Campus<br>
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Near Harringhata Farm<br>
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Mohanpur, Dist: Nadia<br>
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West Bengal, India-741246<br><br><br><br>
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<b>Visit Us Virtually:</b><br><br>
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<a href='https://2018.igem.org/Team:IISER-Kolkata'>iGEM IISER Kolkata</a><br>
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<a href='https://www.iiserkol.ac.in'>IISER Kolkata</a>
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<p id='write'>
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<b>Write to Us:</b><br><br>
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psray_AT_iiserkol_DOT_ac_DOT_in<br>
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Dr. Partho Sarothi Ray<br>
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Primary PI<br><br>
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arnab_DOT_gupta_AT_iiserkol_DOT_ac_DOT_in<br>
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Dr. Arnab Gupta<br>
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Secondary PI<br><br>
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ppr16ms055_AT_iiserkol_DOT_ac_DOT_in<br>
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Paresh Priyadarshan Rana<br>
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Team Leader<br><br>
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team_DOT_igem_AT_iiserkol_DOT_ac_DOT_in<br>
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Team iGEM IISER-Kolkata Official Mail Address
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<b>Facebook:</b><br>
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<a href='https://www.facebook.com/iGEM.IISERK/'>https://www.facebook.com/iGEM.IISERK/</a><br><br>
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<b>Twitter:</b><br>
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<a href='https://twitter.com/iGEM_IISERKol'>https://twitter.com/iGEM_IISERKol</a>
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<div id="footer"><p>Website created by Pavan Vynatheya and Prajwal Padmanabha</p></div>
 
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Latest revision as of 05:32, 24 November 2018

BacMan

A probiotic bacterial BATMAN to protect the citizens of Gotham from Arsenic Poisoning

Visit us Physically

Lab Number 216, Research Complex Building
IISER Kolkata Campus
Near Harringhata Farms
Mohanpur, Dist: Nadia
West Bengal, India-741246

Visit us virtually

IISER Kolkata website

Write to us

Dr. Partho Sarothi Ray (Primary PI)
Dr. Arnab Gupta (Secondary PI)
Paresh Rana (Team Leader)
Team iGEM IISER-Kolkata

Talk to us

Paresh Rana : +91-9078203201
Animish Nilesh Vaze : +91-9273101997

M N