Difference between revisions of "Team:ShanghaiTech/Part Collection"

 
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    <p style="font-family: 'Tw Cen MT'; font-weight: bold; font-size: 35pt">Collection</p>
  
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    <p>We mutated several host RBS (ribosome binding site) and host 16s rRNA to get four orthogonal ribosome systems based on the sequences we got. By combining these orthogonal RBS and orthogonal 16s rRNA, we tested their orthogonality to the host ribosomes and to each other. The results showed significant decrease in the interference of exogenous target gene, which is GFP in this case, and host genes, proving the function of the orthogonal ribosome. Since we would like to introduce the orthogonal ribosome to the iGEM community, we develop a toolkit plasmid that contains the report gene GFP under the orthogonal RBS. The superiority of it is that we have also added restriction enzyme cutting sites before and after the GFP sequence. Hence other users can exchange GFP to their target gene easily and select the right clone by simply detecting the fluorescence intensity of the cells. </p>
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<h1> Part Collection </h1>
 
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
 
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While you should put all the characterization information for your parts on the Registry, you are encouraged to explain how all your parts form a collection on this page.
 
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<h3>Note</h3>
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<p>This page should list all the parts in the collection your team made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page.</p>
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<h3>Best Part Collection Special Prize</h3>
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<p>To be eligible for this award, these parts must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a collection of parts you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part numbers to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.</p>
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Latest revision as of 15:48, 25 November 2018

ShanghaiTech iGEM

Collection

We mutated several host RBS (ribosome binding site) and host 16s rRNA to get four orthogonal ribosome systems based on the sequences we got. By combining these orthogonal RBS and orthogonal 16s rRNA, we tested their orthogonality to the host ribosomes and to each other. The results showed significant decrease in the interference of exogenous target gene, which is GFP in this case, and host genes, proving the function of the orthogonal ribosome. Since we would like to introduce the orthogonal ribosome to the iGEM community, we develop a toolkit plasmid that contains the report gene GFP under the orthogonal RBS. The superiority of it is that we have also added restriction enzyme cutting sites before and after the GFP sequence. Hence other users can exchange GFP to their target gene easily and select the right clone by simply detecting the fluorescence intensity of the cells.


ShanghaiTech iGEM @ 2018