Difference between revisions of "Team:BioIQS-Barcelona/Results"

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                                     <h4 class="book orange-medium">Computationally demonstrated that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes or that a specific DQ2- or DQ8-primer has to be used.</h4>
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                                     <h4 class="book orange-medium">Computationally <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Standardization">demonstrated</a> that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes or that a specific DQ2- or DQ8-primer could be used, reducing the cost of the sensor.</h4>
 
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                                     <h4 class="book orange-medium">Successfully designed a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all the HLA-DQ variants possible with only one protocol.</h4>
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                                     <h4 class="book orange-medium">Successfully <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Basic_Part">designed</a> a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all the HLA-DQ variants possible with only one protocol.</h4>
 
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                                     <h4 class="book orange-medium">Thoroughly created different synthetic constructs to ensure the correct expression of the HLA-DQ in a bacterial or yeast host. This would enable us to find the best way to use them in a sensor.</h4>
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                                     <h4 class="book orange-medium">Thoroughly designed and characterized multiple <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Composite_Part">composite parts</a> to ensure the correct expression of the human HLA-DQ receptor in a bacterial or yeast host. This would enable us to determine the best way to produce de sensor.</h4>
 
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                                     <h4 class="book orange-medium">Carefully built a theoretical model to illustrate the essential mechanism of the sensor. Modelling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.</h4>
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                                     <h4 class="book orange-medium">Carefully built a <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Model">theoretical model</a> to illustrate the essential detection mechanism of the sensor. Modelling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.</h4>
 
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Revision as of 00:28, 26 November 2018

BIO IQS

Project | Results

Have a look!

Achievements of our project

1.

Computationally demonstrated that primer binding regions are perfectly conserved or slightly different for both DQ2 and DQ8 celiac haplotypes separately. This means that either the same primer can be used for amplifying all genotypes or that a specific DQ2- or DQ8-primer could be used, reducing the cost of the sensor.

2.

Successfully designed a simple and cheap way to extract the extracellular domain of both alpha and beta chains of the HLA-DQ protein from the human genome. This would make the extraction of all the HLA-DQ variants possible with only one protocol.

3.

Thoroughly designed and characterized multiple composite parts to ensure the correct expression of the human HLA-DQ receptor in a bacterial or yeast host. This would enable us to determine the best way to produce de sensor.

4.

Correctly cloned one of the constructs into a desired expression vector and prepared it for further expression experiments. Cloning is a tedious work and not always results as one would expect so this is an important achievement!

5.

Carefully built a theoretical model to illustrate the essential detection mechanism of the sensor. Modelling has become one of the most important jobs in this project and have allowed us to better understand the role of the sensor.