Difference between revisions of "Team:Kyoto/Safety"

 
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<body>
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  <div id="jump"><a href="#wrapper"><img src="https://static.igem.org/mediawiki/2017/c/c5/Kyoto_notebook_jump.png"></a></div>
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<div class="clear"></div>
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  <div id="BACKGROUND">
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<div id="jump">
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  <div id="wrapper">
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  <h1>Safety</h1>
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  <ul class="material">
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      <li><a href="#Parts">1) Parts</a></li>
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      <li><a href="#Primer">2) Primer list</a></li>
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      <li><a href="#Materials">3) Materials</a></li>
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        <ul>
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<li><a href="#Kit">3-1 Kit</a></li>
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<li><a href="#Restriction Enzyme">3-2 Restriction Enzyme</a></li>
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<li><a href="#Polymerase">3-3 Polymerase</a></li>
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<li><a href="#DNA ligase">3-4 DNA ligase</a></li>
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<li><a href="#Marker">3-5 Marker</a></li>
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<li><a href="#Organism">3-6 Organism</a></li>
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<li><a href="#Antibiotics">3-7 Antibiotics</a></li>
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<li><a href="#Equipment">3-8 Equipment</a></li>
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<li><a href="#Backbone">3-9 Backbone</a></li>
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<li><a href="#Buffer">3-10 Buffer</a></li>
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<li><a href="#SDS-PAGE&Westernblotting">3-11 SDS-PAGE&Westernblotting</a></li>
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<!--------------------------------------------------------- BLOCK Contents-2 Materials  END----------------------------------------------------------->
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        </ul>
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  <li><a href="#methods">4) Methods</a></li>
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    <ul>
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<!--------------------------------------------------------- BLOCK Contents-3 Methods PY------------------------------------------------------------>
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<a href="#wrapper">
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<li><a href="#Miniprep">4-1 Miniprep</a></li>
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<img src="https://static.igem.org/mediawiki/2018/1/11/T--Kyoto--upbotton.jpg"></a></div>
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<li><a href="#Gel Extraction and PCR purification">4-2 Gel Extraction and PCR purification</a></li>
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<div id="BACKGROUND">
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<li><a href="#Restriction Enzyme Digestion">4-3 Restriction Enzyme Digestion</a></li>
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<li><a href="#Ligation">4-4 Ligation</a></li>
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<li><a href="#Transformation">4-5 Transformation</a></li>
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<!--------------------------------------------------------- BLOCK Contents-3 Methods  END------------------------------------------------------------>
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  </ul>
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<!-- ######################################################  BLOCK Contents END #################################################-->
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<div style='padding-top: 100px;'><h1 id="wrapper"><img src="https://static.igem.org/mediawiki/2018/6/6b/T--Kyoto--safty.png" width="30%"></div></h1>
  
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<!-----------------------------------------------------BLOCK Parts-------------------------------------------------------------->
 
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<h5 id="Parts">1) Parts</h5>
 
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<h6><a href="https://2017.igem.org/Team:Kyoto/Basic_Part" target="brank">Basic Parts</a></h6>
 
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<h6><a href="https://2017.igem.org/Team:Kyoto/Composite_Part" target="brank">Composite Parts</a></h6>
 
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<!-----------------------------------------------------BLOCK Parts END---------------------------------------------------------->
 
  
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<div class="box27">
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    <span class="box-title"><font face="Segoe UI">Table of contents</font></span>
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    <ul class="index1">
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            <li><a href="#Safe project design"><font face="Segoe UI">1) Safe project design</font></a></li>
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            <li><a href="#Safe lab work"><font face="Segoe UI">2) Safe lab work</font></a></li>
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            <li><a href="#Safe shipment"><font face="Segoe UI">3) Safe shipment</font></a></li>
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            <li><a href="#Link"><font face="Segoe UI">4) Link</font></a></li>
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</ul>
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<!------------------------------------------------------BLOCK primer PY---------------------------------------------------------->
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<h5 id="Safe project design">1)Safe project design</h5>
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<br>
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<p><font size="4">All of the species we expect to use are not harmful for human.</font></p>
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<!-----------------------------------------------------BLOCK Safe project design END---------------------------------------------------------->
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<br><br><br>
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<!-- Table Generated by TableMaker.py Author: OHAD -->
 
<!-- Table Generated by TableMaker.py Author: OHAD -->
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<h5 id = "Primer">2) Primer List</h5>
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<h5 id="Safe lab work">
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<div class="example">
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2)Safe lab work</h5>
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<table width="20px">
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<tr><th>primer name</th><th class="example" width=40%>sequence</th><th width=8%>Length</th><th width=5%>Tm</th><th width=8%>GC%</th><th width=10%>Designer</th><th>Manufacturer</th></tr><tr><td>tropomyosinF</td><td>GATCGAGAAGGACAACGCCC</td><td>20</td><td>65</td><td>60</td><td>Fukuda</td><td>Macrogen Japan Corp</td></tr><tr><td>IK107</td><td>cccgccgccaccatggaggcggccgcaaaatcagg</td><td>35</td><td>94</td><td>71</td><td>Yoshimoto</td><td>Macrogen Japan Corp</td></tr></table>
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<br>
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</div>   <!-- Table end -->
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<p><font size="4">
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    Our experiments are normal molecular biology experiment and does not include special dangerous steps. We will properly follow how to use equipment such as autoclave and centrifuge, and will conduct experiments safely. We treat yeasts, bacteria etc. created by observing the safety guidelines and sterilize after the experiment is over. We will collect all laboratory effluent and culture waste liquid, completely sterilize and detoxify them by prescribed method so as not to let any E. coli and yeast that we made to the natural environment after collection out. For organic solvents, after collection, we ask for treatment at university's environmental safety center. The microorganisms we make do not have infectivity to humans, but for safety we wear gloves and safety glasses for all experiments.
 +
<br><br>
 +
    Prof.Kitabatake, Prof.Woltjen, Prof.Sota and Prof.Ohtan are responsible for all experiments and overseeing the whole process of the experiment. Before the experiment, students participate in a safety workshop held in the university and are undergoing training to conduct safe experiments. Experiments using Escherichia coli and yeast are instructed by experts who are experimenting with such a species in a daily scale.
 +
<br><br>
 +
    We will conduct all experiments safely following the safety guideline of Kyoto university. For gene recombination experiments, we observe Kyoto university safety and occupational health reference manual 2018 (<a href="https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing">https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing</a>) and do so.
 +
<br><br>
 +
    We are registered with the Kyoto University Recombinant DNA Committee and the University follows the guidelines of the Cartagena Protocol on Biosafety to the Convention on Biological Diversity.
 +
</font></p>
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<br><br><br>
  
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<h5 id="Safe shipment">3)Safe shipment</h5>
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<!------------------------------------------------------BLOCK Safe shipment PY------------------------------------------------------>
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<!-- Table Generated by TableMaker.py Author: OHAD -->
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<br>
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<p><font size="4">Before submission, we have read all related pages in the iGEM web page.</font></p>
  
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<h5 id="Materials">3) Materials</h5>
 
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<h6 id = "Kit">3-1 Kit</h6>
 
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<table>
 
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<tr><th>Name</th><th>Supplier</th></tr><tr><td>Wizard&#174; SV Gel and PCR</td><td>Promega</td></tr><tr><td>FastGene™Plasmid Mini Kit</td><td>NIPPON Genetics Co.,Ltd</td></tr>
 
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<!-- Table end -->
 
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<br><br><br>
  
  
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<h5 id="methods">4) Methods</h5>
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<h5 id="Link">4)Link</h5>
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<h6 id = "Miniprep">4-1 Miniprep</h6>
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<p><font size="4" color="#00252A">1.Final safety form</font></p>
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<p>
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<p><font size="4"><a href="https://2018.igem.org/Safety/Final_Safety_Form?team_id=2665"> Safety form</a></font></p>
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Minipreps were performed using FastGene&#8482;Plasmid Mini Kit and and Wizard® Plus SV Minipreps DNA Purification System according to the manufacturer's protocols.
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</p>
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<h6 id = "Soaking">4-17 Soaking</h6>
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<p>We followed this paper as for soaking.<br>
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  <a href="https://link.springer.com/content/pdf/10.1007%2Fs10658-012-0035-0.pdf">https://link.springer.com/content/pdf/10.1007%2Fs10658-012-0035-0.pdf</a></p>
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</ol>
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<br>
 
<br>
 +
<p><font size="4" color="#00252A">2.Kyoto university safety and occupational health reference manual 2018</font></p>
 +
<p><a href="https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing">
 +
<font size="4">https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing</font></a></p>
 
<br>
 
<br>
 
<!-- ~~~~~~~~~~~~~Part END~~~~~~~~~~~~~~ -->
 
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Latest revision as of 03:16, 27 November 2018

Table of contents



1)Safe project design

All of the species we expect to use are not harmful for human.




2)Safe lab work

Our experiments are normal molecular biology experiment and does not include special dangerous steps. We will properly follow how to use equipment such as autoclave and centrifuge, and will conduct experiments safely. We treat yeasts, bacteria etc. created by observing the safety guidelines and sterilize after the experiment is over. We will collect all laboratory effluent and culture waste liquid, completely sterilize and detoxify them by prescribed method so as not to let any E. coli and yeast that we made to the natural environment after collection out. For organic solvents, after collection, we ask for treatment at university's environmental safety center. The microorganisms we make do not have infectivity to humans, but for safety we wear gloves and safety glasses for all experiments.

Prof.Kitabatake, Prof.Woltjen, Prof.Sota and Prof.Ohtan are responsible for all experiments and overseeing the whole process of the experiment. Before the experiment, students participate in a safety workshop held in the university and are undergoing training to conduct safe experiments. Experiments using Escherichia coli and yeast are instructed by experts who are experimenting with such a species in a daily scale.

We will conduct all experiments safely following the safety guideline of Kyoto university. For gene recombination experiments, we observe Kyoto university safety and occupational health reference manual 2018 (https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing) and do so.

We are registered with the Kyoto University Recombinant DNA Committee and the University follows the guidelines of the Cartagena Protocol on Biosafety to the Convention on Biological Diversity.




3)Safe shipment

Before submission, we have read all related pages in the iGEM web page.





1.Final safety form

Safety form


2.Kyoto university safety and occupational health reference manual 2018

https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing