Difference between revisions of "Team:Kyoto/Safety"

(Prototype team page)
 
 
(49 intermediate revisions by 4 users not shown)
Line 1: Line 1:
 
{{Kyoto}}
 
{{Kyoto}}
 +
{{Kyoto/header}}
 
<html>
 
<html>
  
 +
<style type="text/css">
  
−
<div class="column full_size">
+
a:hover { text-decoration:underline;
 +
}
 +
        a:link {color:#007081;
 +
        }
 +
        a:visited {color:#ffbe4b;
 +
        }
 +
        a:hover {color:#25B6CA;
 +
        }
 +
        a:active {color:#FFFC7A;
 +
        }
  
−
<h1> Safety </h1>
 
−
<p>Please visit the <a href="https://2018.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
−
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
+
 +
  ul.index1 {
 +
    list-style:none;
 +
    text-align:left;
 +
    font-family: 'Segoe UI';
 +
    font-size:140%;
 +
    margin-top: 15px;
 +
    margin-left:10%;
 +
    margin-right:10%;
 +
    margin-bottom: 10px;
 +
    color: #f0f8ff;
 +
  }
 +
  ul.index1 ul{
 +
    list-style:none;
 +
   
 +
  }
 +
  li{
 +
    list-style:none;
 +
  }
 +
  .box27 {
 +
    margin-top: 0;margin-left: 0;
 +
    margin-right: 0;margin-bottom: 0;;
 +
    background: #25B6CA;
 +
    padding: 0px 5px 5px 5px;
 +
 
 +
}
 +
  .box27 .box-title {
 +
    position: relative;
 +
    display: inline-block;
 +
    top: 7px;
 +
    margin-left:10%;
 +
    padding: 0 15px ;
 +
    height: 25px;
 +
    line-height: 25px;
 +
    vertical-align: middle;
 +
    font-size: 240%;
 +
    background:  #25B6CA;
 +
    color: #ffffff;
 +
    font-weight: bold;
 +
    border-radius: 5px 5px 0 0;
 +
}
 +
  .box27 p {
 +
    margin: 0;
 +
    padding: 0;
 +
}
 +
  p.description{
 +
    text-align:center;
 +
    margin-left:0 auto;
 +
    margin-right:0 auto;}
 +
  p.description2{
 +
    text-align:center;
 +
    margin-left:47%;
 +
    margin-right:10%;
 +
  }
  
−
</div>
+
p {font-family: 'Segoe UI';
 +
  }
  
  
−
<div class="column two_thirds_size">
+
h1{ margin: 15px;
−
<h3>Safe Project Design</h3>
+
  }
  
−
<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
+
h2 {
 +
background: linear-gradient(transparent 90%, #25B6CA 80%);
 +
margin-left: 10%;
 +
}
  
−
<ul>
+
h5 {
−
<li>Choosing a non-pathogenic chassis</li>
+
background: linear-gradient(transparent 90%, #25B6CA 80%);
−
<li>Choosing parts that will not harm humans / animals / plants</li>
+
margin-left: 10%;
−
<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
+
}
−
<li>Including an "induced lethality" or "kill-switch" device</li>
+
−
</ul>
+
  
−
</div>
 
  
−
<div class="column third_size">
+
#jump{
−
<h3>Safe Lab Work</h3>
+
    position:fixed;
 +
    bottom:5%;
 +
    right:0;
 +
    width:9%;
 +
}
 +
#jump img{
 +
    width:70%;
 +
}
 +
  ul.material {
 +
    margin-left:10%;
 +
    margin-right:10%;
 +
    list-style:none;
 +
    text-align:left;
 +
    font-family: serif, 'Times New Roman';
 +
    font-size:150%;
 +
  }
 +
  ul.material ul{
 +
    list-style:none;
 +
  }
 +
  .material > p {
 +
    word-wrap:break-word;
 +
  }
 +
    table{
 +
    color:#000000;
 +
    border-collapse:collapse;
 +
    border-left:1px solid #000000;
 +
    border-top:1px solid #000000;
 +
    margin-top:30px;
 +
    width:95%;
 +
    table-layout:fixed;
 +
  }
  
−
<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
+
  th{
 +
    border-right:1px solid #ffffff;
 +
    border-bottom:1px solid #ffffff;
 +
    background-color:#556c14;
 +
    color:#ffffff;
 +
  }
  
 +
  td{
 +
    border-right:1px solid #000000;
 +
    border-bottom:1px solid #000000;
 +
    word-wrap:break-word;
 +
  }
 +
  p{font-size:100%;}
 +
td.pcr{border-left:0;
 +
    border-top:0;
 +
    background-color:#e8f0d2;}
  
−
<h3>Safe Shipment</h3>
+
</style>
 +
       
 +
<body>
 +
<div class="clear"></div>
 +
<div id="jump">
  
−
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
+
<a href="#wrapper">
 +
<img src="https://static.igem.org/mediawiki/2018/1/11/T--Kyoto--upbotton.jpg"></a></div>
 +
<div id="BACKGROUND">
 +
 
 +
 
 +
<div style='padding-top: 100px;'><h1 id="wrapper"><img src="https://static.igem.org/mediawiki/2018/6/6b/T--Kyoto--safty.png" width="30%"></div></h1>
 +
 
 +
 
 +
<div class="box27">
 +
    <span class="box-title"><font face="Segoe UI">Table of contents</font></span>
 +
    <ul class="index1">
 +
            <li><a href="#Safe project design"><font face="Segoe UI">1) Safe project design</font></a></li>
 +
            <li><a href="#Safe lab work"><font face="Segoe UI">2) Safe lab work</font></a></li>
 +
            <li><a href="#Safe shipment"><font face="Segoe UI">3) Safe shipment</font></a></li>
 +
            <li><a href="#Link"><font face="Segoe UI">4) Link</font></a></li>
 +
           
 +
</ul>
 
</div>
 
</div>
  
  
 +
 +
<!-- ######################################################  BLOCK Contents END #################################################-->
 +
 +
<br><br><br>
 +
<!-----------------------------------------------------BLOCK Safe project design-------------------------------------------------------------->
 +
<h5 id="Safe project design">1)Safe project design</h5>
 +
<br>
 +
<p><font size="4">All of the species we expect to use are not harmful for human.</font></p>
 +
<!-----------------------------------------------------BLOCK Safe project design END---------------------------------------------------------->
 +
<br><br><br>
 +
<!------------------------------------------------------BLOCK Safe lab work---------------------------------------------------------->
 +
<!-- Table Generated by TableMaker.py Author: OHAD -->
 +
<h5 id="Safe lab work">
 +
2)Safe lab work</h5>
 +
 +
<br>
 +
<p><font size="4">
 +
    Our experiments are normal molecular biology experiment and does not include special dangerous steps. We will properly follow how to use equipment such as autoclave and centrifuge, and will conduct experiments safely. We treat yeasts, bacteria etc. created by observing the safety guidelines and sterilize after the experiment is over. We will collect all laboratory effluent and culture waste liquid, completely sterilize and detoxify them by prescribed method so as not to let any E. coli and yeast that we made to the natural environment after collection out. For organic solvents, after collection, we ask for treatment at university's environmental safety center. The microorganisms we make do not have infectivity to humans, but for safety we wear gloves and safety glasses for all experiments.
 +
<br><br>
 +
    Prof.Kitabatake, Prof.Woltjen, Prof.Sota and Prof.Ohtan are responsible for all experiments and overseeing the whole process of the experiment. Before the experiment, students participate in a safety workshop held in the university and are undergoing training to conduct safe experiments. Experiments using Escherichia coli and yeast are instructed by experts who are experimenting with such a species in a daily scale.
 +
<br><br>
 +
    We will conduct all experiments safely following the safety guideline of Kyoto university. For gene recombination experiments, we observe Kyoto university safety and occupational health reference manual 2018 (<a href="https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing">https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing</a>) and do so.
 +
<br><br>
 +
    We are registered with the Kyoto University Recombinant DNA Committee and the University follows the guidelines of the Cartagena Protocol on Biosafety to the Convention on Biological Diversity.
 +
</font></p>
 +
  <!-- Table end -->
 +
   
 +
<!------------------------------------------------------BLOCK Safe lab work END---------------------------------------------------------->
 +
 +
<br><br><br>
 +
 +
<h5 id="Safe shipment">3)Safe shipment</h5>
 +
<!------------------------------------------------------BLOCK Safe shipment PY------------------------------------------------------>
 +
<!-- Table Generated by TableMaker.py Author: OHAD -->
 +
<br>
 +
<p><font size="4">Before submission, we have read all related pages in the iGEM web page.</font></p>
 +
 +
<!-- Table end -->
 +
<!------------------------------------------------------BLOCK Safe shipment.py END------------------------------------------------------>
 +
<br><br><br>
 +
 +
 +
<h5 id="Link">4)Link</h5>
 +
<!------------------------------------------------------BLOCK Link PY---------------------------------------------------------------->
 +
<br>
 +
<!-- ##############The below part of block is Generated by Methods.py Author: OHAD################# -->
 +
<p><font size="4" color="#00252A">1.Final safety form</font></p>
 +
<p><font size="4"><a href="https://2018.igem.org/Safety/Final_Safety_Form?team_id=2665"> Safety form</a></font></p>
 +
<br>
 +
<p><font size="4" color="#00252A">2.Kyoto university safety and occupational health reference manual 2018</font></p>
 +
<p><a href="https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing">
 +
<font size="4">https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing</font></a></p>
 +
<br>
 +
<!-- ~~~~~~~~~~~~~Part END~~~~~~~~~~~~~~ -->
 +
 +
<!------------------------------------------------------BLOCK Link.py END -----------------------------------------------------------><br><br><br>
 +
      </div>
 +
  </div>
 +
 +
 +
</p>
 +
<!--
 +
NewPP limit report
 +
CPU time usage: 0.007 seconds
 +
Real time usage: 0.007 seconds
 +
Preprocessor visited node count: 9/1000000
 +
Preprocessor generated node count: 42/1000000
 +
Post‐expand include size: 41/2097152 bytes
 +
Template argument size: 0/2097152 bytes
 +
Highest expansion depth: 2/40
 +
Expensive parser function count: 0/100
 +
-->
 +
 +
<!-- Saved in parser cache with key 2017_igem_org:pcache:idhash:1170-0!*!*!*!*!*!* and timestamp 20171027171725 and revision id 271514
 +
-->
 +
</div>             <div class="visualClear"></div>
 +
            </div>
 +
    </div>
 +
 +
        </div>
 +
 +
        <!-- Side Menubar -->
 +
        <div id="sideMenu">
 +
            <a href="https://2017.igem.org">
 +
                <div id="home_logo" >
 +
                <img src="https://static.igem.org/mediawiki/2017/b/bf/HQ_menu_logo.jpg">
 +
                </div>
 +
            </a>
 +
 +
            <div style="clear:both; height:5px;"></div>
 +
 +
            <div id="menuDisplay"></div>  <!- Menu will be loaded here ->
 +
        </div>
 +
 +
        <!-- Pop_Why Div is definded here -->
 +
        <div class="pop_why_cover"></div>
 +
 +
        <div class="pop_why_box" >
 +
            <div class="pop_close">× </div>
 +
            <div class="pop_why_content"><h3> Loading ... </h3></div>
 +
 +
    </div>
 +
 +
    </div>
 +
  </body>
 
</html>
 
</html>

Latest revision as of 03:16, 27 November 2018

Table of contents



1)Safe project design

All of the species we expect to use are not harmful for human.




2)Safe lab work

Our experiments are normal molecular biology experiment and does not include special dangerous steps. We will properly follow how to use equipment such as autoclave and centrifuge, and will conduct experiments safely. We treat yeasts, bacteria etc. created by observing the safety guidelines and sterilize after the experiment is over. We will collect all laboratory effluent and culture waste liquid, completely sterilize and detoxify them by prescribed method so as not to let any E. coli and yeast that we made to the natural environment after collection out. For organic solvents, after collection, we ask for treatment at university's environmental safety center. The microorganisms we make do not have infectivity to humans, but for safety we wear gloves and safety glasses for all experiments.

Prof.Kitabatake, Prof.Woltjen, Prof.Sota and Prof.Ohtan are responsible for all experiments and overseeing the whole process of the experiment. Before the experiment, students participate in a safety workshop held in the university and are undergoing training to conduct safe experiments. Experiments using Escherichia coli and yeast are instructed by experts who are experimenting with such a species in a daily scale.

We will conduct all experiments safely following the safety guideline of Kyoto university. For gene recombination experiments, we observe Kyoto university safety and occupational health reference manual 2018 (https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing) and do so.

We are registered with the Kyoto University Recombinant DNA Committee and the University follows the guidelines of the Cartagena Protocol on Biosafety to the Convention on Biological Diversity.




3)Safe shipment

Before submission, we have read all related pages in the iGEM web page.





1.Final safety form

Safety form


2.Kyoto university safety and occupational health reference manual 2018

https://drive.google.com/file/d/1JyKun0qIsp6O1ts2nJGPSdt23NKrSfBQ/view?usp=sharing