Difference between revisions of "Team:CCU Taiwan/engaging experts"

 
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'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
 
'https://2018.igem.org/Team:CCU_Taiwan';"> <img src="https://static.igem.org/mediawiki/2018/0/08/T--CCU_Taiwan--home_button.png"></img></div>
  
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                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr>
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                     <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a>
 
                         <ul class="sub" id="sub_home" style="cursor:default;">
 
                         <ul class="sub" id="sub_home" style="cursor:default;">
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home2">Medals</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home3">Medals</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home3">For Judges</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home4">For Judges</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Achievements"><li class="list" id="home5">Achievements</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
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                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr>
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                     <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a>
 
<ul class="sub" id="sub_project" style="cursor:default;">
 
<ul class="sub" id="sub_project" style="cursor:default;">
 
    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
 
    <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project4">Demonstration</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab"><li class="list" id="project5">InterLab</li></a>
 
</ul>
 
</ul>
 
</li>
 
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                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr>
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                     <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a>
 
                         <ul class="sub" id="sub_parts" style="cursor:default;">
 
                         <ul class="sub" id="sub_parts" style="cursor:default;">
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts1">Basic Part</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts2">Composite Part</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Improve"><li class="list" id="parts3">Improved Part</li></a>
 
 
                         </ul>
 
                         </ul>
 
                     </li>
 
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                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr>
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                     <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a>
 
                         <ul class="sub" id="sub_modeling" style="cursor:default;">
 
                         <ul class="sub" id="sub_modeling" style="cursor:default;">
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding Model</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model3">Polymer Model</li></a>
 
                         </ul>
 
                         </ul>
 
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                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a><hr>
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                     <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a>
 
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
 
                         <ul class="sub" id="sub_drylab" style="cursor:default;">
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
 
                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a>
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                         </ul>
 
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr>
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    <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a>
 
                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
 
                         <ul class="sub" id="sub_human_practice" style="cursor:default;">
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a>
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                             <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Human Practice</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
 
<a href="https://2018.igem.org/Team:CCU_Taiwan/engaging_experts"><li class="list" id="human_practice4">Engaging Experts</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate"><li class="list" id="human_practice5">Intergrated HP</li></a>
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<a href="https://2018.igem.org/Team:CCU_Taiwan/Integrate"><li class="list" id="human_practice5">Integrated HP</li></a>
 
                         </ul>
 
                         </ul>
 
                     </li>
 
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     </header>
 
     </header>
 +
 +
 +
<div class="indicator">
 +
 +
<div class="pointerHP" id="1"><a href="#ca1">Gene Design</a></div>
 +
<div class="pointerHP" id="2"><a href="#ca2">Material Properties</a></div>
 +
<div class="pointerHP" id="7"><a href="#ca7">Taiwan NCHU</a></div>
 +
<div class="pointerHP" id="itri"><a href="#ca3">Production Line</a></div>
 +
<div class="pointerHP" id="4"><a href="#ca4">Recycling Program</a></div>
 +
<div class="pointerHP" id="6"><a href="#ca6">Manufacturers</a></div>
 +
<div class="pointerHP" id="5"><a href="#ca5">Enterpreneumship</a></div>
 +
 +
</div>
  
 
<div class="backgroundHP">
 
<div class="backgroundHP">
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<div class="photoEngagingExperts"></div>
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<div class="photoEngagingExperts"><h1 class="bigtitle">ENGAGING EXPERTS<h1></div>
 
       <div class="content">
 
       <div class="content">
−
<p class="first">Entrepreneurship</p>
+
<br>
−
<p class="second">Bobsen:</p>
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<p class="first" id="ca1">Gene Design</p>
−
<p class="description">&emsp;&emsp;Bobsen is a paper container laminated manufacturer, after our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line.<br>Followings are information we got form Bobsen:
+
<p class="second">National Taiwan University</p>
−
<br>Mails:
+
−
<br>Corporate Visit Notes:
+
−
</p>
+
−
<hr>
+
−
<p class="second">Chung Hwa Pulp Corporation:</p>
+
−
<p class="description">&emsp;&emsp;Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper and the black liquor. In addition, they recommended that we can ask experts from ITRI (Industrial Technology Research Institute) and professors at Chung-Hsing University for more questions about the characteristics of paper container raw materials. <br><br>
+
−
Following are questions we had asked:<br>
+
−
1. What’s the raw material you use to produce paper?<br>
+
−
2. We knew that “black liquor” would be produced during the procedure of paper manufacturing. What’s the percentage of lignin in black liquor? Is there a technology for recovering lignin or is there any other manufacturers recycling and purifying the black liquor?<br>
+
−
3. Would it have any influence on paper recycling if the paper is doped with lignin?<br><br>
+
  
−
The answers they gave us:<br>
+
                <div id="EX1" class="polaroid">
−
1. The raw material of the paper comes from the pulp, which consists of cellulose and hemicellulose separated from the wood chips.<br>
+
                  <img src="https://static.igem.org/mediawiki/2018/4/49/T--CCU_Taiwan--JOE_experts_01.jpg" style="width:100%">
−
2. The black liquor is produced after the wood chips are cooked, so the black liquor component is lignin. Black liquor will be concentrated and used as a biomass fuel to provide the energy needed for production.<br>
+
                </div>
−
3. You can find paper quality and technology in the relevant literature.<br>
+
 
−
The process of pulping and papermaking is described as follows:<br>
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<p class="second">Professor K.S. Tung</p>
−
1. The process of pulping:<br>
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<p class="description">Institute of Molecular and Cellular Biology<br>National Taiwan University</p>
−
The main raw material of the paper is wood pulp, which is made by peeling and slicing the logs. The principle is to separate the wood chips from the pulp fibers by mechanical or chemical methods, and then the fibers are pulped, bleached and dried to make pulp or pulp board.<br><br>
+
 
−
→ Cooking: Digestion is also called cooking. The purpose is to dissolve the lignin in the wood chips by using chemicals and under high-temperature cooking conditions to dissociate the fibers.<br>
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                <div id="EX2" class="polaroid">
−
→ Bleaching: The color of the pulp after cooking is generally tawny, which is mostly used in packaging kraft. For high grade paper, the color needs to be white, so that the printed pictures and texts are vivid. In order to improve the whiteness of the pulp, it must be bleached.<br><br>
+
                  <img src="https://static.igem.org/mediawiki/2018/d/d5/T--CCU_Taiwan--JOE_experts_02.jpg" style="width:100%">
−
2. The process of papermaking:
+
                </div>
−
The process of paper producing is made of wood pulp or paperboard, through pulping, beating, batching, cleaning, screening, molding, dehydration and drying, then the products can be used for household paper and high-grade cultural paper. If recycled paper is used as the raw material, the process must go through deinking, which is mention to remove the printing ink and impurities on the surface of the recycled paper, process it into recycled pulp and then produce the product through the papermaking process, such products are mostly used for photocopying papers, newspapers, general culture printing paper and cardboard.<br>
+
<br><br>
 +
<p class="description">&emsp;&emsp;Professor K.S. Tung provided us constitutive type of promoter (ADH1 promoter), and auxotrophic marker (LEU2, URA3, TRP1).</p><br><br><br>
 +
<p class="second">Professor C.T. Huang</p>
 +
<p class="description">Department of Biochemical Science and Technology<br>
 +
National Taiwan University
 
</p>
 
</p>
−
<hr>
 
−
<p class="description">ITRI (Industrial Technology Research Institute):<br>
 
−
Through the introduction of Chung Hwa Pulp Corporation, we found ITRI. Dr. Hsieh is an expert specializing in the field of lignin. Since LIGGREEN is a new product similar to lignin, we would like to benefit from Dr. Hsieh, hoping to understand more about the characteristics that LIGGREEN may need or avoid during processing.<br>
 
−
Besides Dr. Hsieh, we also asked Dr. Huang and Dr. Hsu to know the direction of LIGGREEN's future development and cater to market needs.<br><br>
 
−
Following are some questions that we had asked Dr. Hsieh:<br>
 
−
1. We have found that there are three kinds of bonds between G monolignols - β-5, β-O-4, β-β, and β-O-4 is the preferred bond with a ratio of up to 50% in the literature. We want to know that if the information we found is correct. We will use UV spectra to observe these bonds to determine its structure, and we also hope to find these bonds.<br>
 
−
Dr. Hsieh: Yes, the cleavage of lignin is also broken from this place.<br><br>
 
−
2. Monolignol is colorless, yet the lignin is brown. How could we explain the differences on color between these two objects? Is there any way to change the color for lignin? According to the literature, we knew that when lignin is exposed to sunshine, the color will fade, as well as the structure of lignin become fragile.<br>
 
−
Dr. Hsieh: I am afraid that I cannot give you more information that how to get the color of lignin. What I can say is that the color of lignin is mainly due to the structure of phenol. In some applications, it is very important not to have color, such as the paper cup you want to use. As for the paint inside the can, the color considerations are relatively small. We had tried to remove the color of lignin, the idea is how to make the structure of phenol disappear. Of course, if you have a way to synthesize lignin, and there is no color, it is also a breakthrough, but the problem is that you have to think about how to judge the structure of your synthesis is lignin? The other is why you should deliberately synthetic lignin? Lignin is attracting attention because it is a material that is readily available in nature, and the amount is huge. The focus is on things that were previously considered as no value. There is a concept of waste utilization, then your team have to think about what is the meaning of deliberately synthesizing lignin? Is color, structurally simple, or is other disadvantages of synthetic lignin without natural lignin? I think you should think twice.<br><br>
 
−
3. The data we have found in the literature now indicate the melting point, density, chemical properties and physical properties of the purified lignin, but these properties will vary due to the large number of lignin species. We want to ask if Doctor can provide the nature of the lignin that you have searched? (Reaction in elastic, acidic solution, etc.) Then we can make the experiment process more smooth.<br>
 
−
Dr. Hsieh: This depends entirely on the source of your lignin. The structure and characteristics of lignin are affected by the species, origin, season, and even the process conditions obtained. This means that if you took an A-source lignin for experimentation and application development, after finding the optimum conditions, however, such conditions have the opportunity not to apply to the B-source of lignin. Of course, if you can develop synthetic lignin may solve this problem.<br><br>
 
−
4. In general pulp and paper manufacturing, NaOH (high-concentration sodium hydroxide) is added to separate lignin from cellulose, and the mixed solution under washing is called black liquor. In the literature, the change of bonding of lignin after the addition of sodium hydroxide makes the proportion of G monolignaols relatively higher. I would like to ask, if we add the enzyme peroxidase16 to black liquor (using the chemical side to adjust the PH to 4-5 and filter, do not destroy the environment of the enzyme), whether it can make the bond that was originally broken by the strong base link again?<br>
 
−
Dr. Hsieh: I cannot answer this question because I am not this doctor of bacteria searching. There is one thing I want to remind you that is that the composition in the black liquor is quite complicated (the compound produced by hemicellulose cracking), so you have to test whether such an enzyme can live in black liquor, and whether the activity will decrease.<br><br>
 
−
5. About the decomposition, we will use Bacillus sp bacteria to decompose lignin in a biodegradable way. If it is a G monolignol polymer, whether this part is feasible?<br>
 
−
Dr. Hsieh: I am sorry that this has exceeded my field, so I cannot give you a better answer.<br><br>
 
−
6. Is lignin in black liquor highly polymerized or small molecule fragments polymerized?<br>
 
−
Dr. Hsieh: The lignin in the black liquor is the lignin of the wood. As you had mentioned above, in the papermaking process, only the strong alkali is used to dissolve the lignin and hemicellulose in the cellulose, and the hemicellulose will deteriorate, but lignin does not degrade into oligomers or even small molecules (chemical processes require further high temperature and pressure to further degrade lignin).<br><br>
 
−
7. If we remove the suspended solids, organic pollutants and toxic substances from black liquor, then add the enzyme Laccase to the black liquor, will it decompose the already polymerized lignin?<br>
 
−
Dr. Hsieh: I checked that Laccase can break down lignin, but I don't know the environment and conditions of decomposition.<br><br>
 
  
  
−
Dr. SHU – CHUAN HUANG:<br>
+
                <div id="EX3" class="polaroid">
−
She recommended that the coverage of the material should be very good. In addition, she recommended that we should refer to the isoo/astm standard, D6400, or the plastic center. Then, she recommended that we should cut the material into small pieces, carry out industrial composting, and detect the decomposition ratio.<br><br>
+
                  <img src="https://static.igem.org/mediawiki/2018/0/0f/T--CCU_Taiwan--JOE_experts_03.jpg" style="width:100%">
 +
                </div>
 +
<br>
 +
<p class="description">&emsp;&emsp;Professor C.T. Huang suggested using constitutive promoter without inducing by methanol. That is GAP promoter which is commonly used in <I>P. pastoris</I>. He also recommended that we could choose commercial pGAPZ A vector from Invitrogen.</p>
 +
 +
<hr>
 +
<p class="first" id="ca2">Material Properties</p>
 +
<p class="second">ITRI (Industrial Technology Research Institute)</p>
 +
                <div id="EX4" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/2/26/T--CCU_Taiwan--JOE_EXPEARTS_NEW01.jpg" style="width:100%">
 +
                </div>
  
−
Dr. SU（Green Energy and Environment Research Laboratories）<br>
+
<br><br><br>
−
He suggested us to make sure that our product is waterproof, and the weight will not exceed the paper cups in market too much.<br>
+
<p class="description">&emsp;&emsp;ITRI is a nonprofit R&D organization engaging in applied research and technical services. Founded in 1973, ITRI has played a vital role in transforming Taiwan's economy from a labor-intensive industry to a high-tech industry.</p><br><br><br>
−
</p>
+
<p class="second">Dr. Cheng-Han Hsieh</p>
 +
                <div id="EX5" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/3/35/T--CCU_Taiwan--JOE_EXPEARTS_NEW02.jpg" style="width:100%">
 +
                </div>
 +
 
 +
<p class="description">(The fourth on the left, Dr. Hsieh)</p><br>
 +
<p class="description">Research Fellow - Lignin Analytics<br><br>
 +
&emsp;&emsp;Dr. Cheng-Han Hsieh recommended that we have to control the LIGGREEN in micromolecular to make it have the melting point. He also gave us some directions for decomposing lignin-like material. The Bacillus subtilis may be our good choice.
 +
</p><br><br><br>
 +
<p class="second">Dr. Shu-Chuan Huang</p>
 +
                <div id="EX6" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/0/06/T--CCU_Taiwan--EXP_1.jpg" style="width:100%">
 +
                </div>
 +
 
 +
<p class="description">(The fourth on the left, Dr. Huang)</p><br>
 +
<p class="description">Research Fellow – Material and Chemical Research<br><br>
 +
&emsp;&emsp;Dr. Shu-Chuan Huang recommended that we should refer to the ISO 9000, ASTM D6400, and so on. Then, she recommended that we should cut the material into small pieces, carry out industrial composting, and detect the decomposition ratio.
 +
</p>
 
<hr>
 
<hr>
−
+
<p class="first" id="ca7">National Chung Hsing University</p>
−
<p class="description">CEO of THEFREEN BURGER：<br>
+
−
&emsp;&emsp;He provides us with more thinking and how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society.<br><br>
+
  
−
Feedback:<br><br>
 
−
&emsp;&emsp;Did you finally mention whether I want to use it? There are two sections. First, the product itself, from a sustainable perspective, is definitely the targets we will be looking forward to in the future. From the practical side, the unit price of 2.5oz $6.5 is far higher than many general paper cups! In Taiwan's catering market, there is a general trend of price orientation. Everyone knows that they should pursue value, but when they return to the basic relationship between supply and demand, they seem to be in a difficult situation.<br>
 
  
−
&emsp;&emsp;You are still in the research and development stage, and the cost is certainly not the primary consideration now. This is very reasonable, but what about the relevant future planning? A sweet price point? A sweet production scale? Can Taiwan’s market be rationally expected within a certain period of time? Or is there a chance to switch to foreign market such as Hong Kong or Tokyo and whether the unit price can be perfectly supported?<br>
+
                <div id="EX7" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/0/05/T--CCU_Taiwan--JOE_experts_07.jpg" style="width:100%">
 +
                </div>
  
−
&emsp;&emsp;Recently, I saw several interesting news. The French agent of Sunrise Tea Tai brought this Taiwanese brand into the Louvre, and even a cup of bubble milk tea sold for more expensive than a cup of Starbucks! Such a market or brand demand may also be another consideration that can be written into your script. The opinions on the Internet (of course, both positive and negative are very important) Perhaps it is also possible to add a reference.<br>
+
<p class="second">Prof. Yuan-Shing Perng</p>
 +
<p class="description">Department of Forestry<br>
 +
National Chung Hsing University
 +
</p>
  
−
&emsp;&emsp;Regarding the government's subsidy policy, some aspects may need to be clarified in this regard. In addition, it is important to understand the market conditions (because our current overall usage and production requirements are not objective, it is recommended that you can refer to the relevant objective price of the relevant package), but how to use the experimental stage and future commercial Properly linked, it is also the key to thinking. As in the other countries' catering pricing and market logic in the letter, how is more likely to accept newer technology trends, or to increase the feasibility of acceptance. Observe and think carefully, it is necessary to learn the key advantages.<br>
+
 
−
</p>
+
                <div id="EX8" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/c/ce/T--CCU_Taiwan--JOE_EXPEARTS_NEW03.jpg" style="width:100%">
 +
                </div>
 +
 
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Prof. Yuan-Shing Perng helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system.
 +
</p><br><br><br>
 +
<p class="second">Prof. Wen-Jau Lee</p>
 +
<p class="description">Department of Forestry<br>
 +
National Chung Hsing University
 +
</p>
 +
 
 +
                <div id="EX9" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/c/c4/T--CCU_Taiwan--JOE_experts_09.jpg" style="width:100%">
 +
                </div>
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Prof. Wen-Jau Lee told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties.
 +
</p>
 
<hr>
 
<hr>
−
<p class="description">Taiwan Recycle Tour<br><br>
+
<p class="first">Recycling System</p>
−
Wu, Meng-Erh Deputy Executive Secretary ：<br>
+
<p class="second">Recycling Fund Management Board</p>
−
He recommended that our products may need to consider the shelf life and the rate of pulping. And he mentioned that the government used the method of Administrative guidance and Subsidy incentive to try to improve the recovery yield. <br>
+
 
−
However, some misunderstandings in the process led to false positives in the news, causing the public to throw paper containers into general garbage disposal.<br>
+
                <div id="EX10" class="polaroid">
−
</p>
+
                  <img src="https://static.igem.org/mediawiki/2018/0/0b/T--CCU_Taiwan--JOE_EXPEARTS_NEW04.jpg" style="width:100%">
 +
                </div>
 +
 +
<p class="second">More Wu</p>
 +
<p class="description">Deputy executive secretary of Recycling Fund Management Board
 +
</p><br>
 +
<p class="description">&emsp;&emsp;More Wu recommended that our products may need to consider the shelf life, because some paper containers such as milk carton are packaging beverage for a long time and the lining of them cannot be leach. And he mentioned that the government used the method of Administrative guidance and Subsidy incentive to try to improve the recovery yield. <br>
 +
However, some misunderstandings in the process led to false positives in the news, causing the public to throw paper containers into general garbage disposal.
 +
</p>
 
<hr>
 
<hr>
−
<p class="description">Professor Shu:<br> <br>
+
<p class="first" id="ca3">Production Line</p>
−
He told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups.<br>
+
<p class="second">Bobsen Paper Corporation</p>
−
</p>
+
 
 +
                <div id="EX11" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/5/58/T--CCU_Taiwan--JOE_experts_11.jpg" style="width:100%">
 +
                </div>
 +
 
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Bobsen Paper Corporation is responsible for producing coated paper and assisting downstream manufacturers in creating creases on paper. After our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line.  
 +
</p>
 
<hr>
 
<hr>
−
<p class="description">Paper Carton Alliance (PCA)：<br> <br>
+
<p class="first" id="ca4">Recycling Program</p>
−
We asked some questions for their exhibition. They supplied us the data reference and defined what the paper containers are.<br>
+
<p class="second">Prof. Ping-Mu Shu</p>
−
</p>
+
<p class="description">Department of Earth and Environmental Science<br>
 +
National Chung Cheng University
 +
</p>
 +
 
 +
 
 +
                <div id="EX12" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/1/14/T--CCU_Taiwan--JOE_experts_12.jpg" style="width:100%">
 +
                </div>
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Prof. Ping-Mu Shu told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups.
 +
</p>
 +
<hr>
 +
<p class="first" id="ca6">Manufacturers & Factories</p>
 +
<p class="second">Lian-Tai</p>
 +
 
 +
 
 +
                <div id="EX13" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/e/e4/T--CCU_Taiwan--JOE_experts_13.jpg" style="width:100%">
 +
                </div>
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Liantai is the only manufacturer with the support from government and controlling the technology to recycle the paper containers and separated paper from the polyethylene lynin.
 +
<br><br>
 +
&emsp;&emsp;The recycling volume of paper containers last year are 25,000 tons, which meant that only 15% of the paper containers had been recycled.
 +
<br><br>
 +
Q:&emsp; Professor Peng had said that the general paper industries could also separate paper from plastic. We wonder to know that why only your manufacture uses the separated technology but not afraid of others replace you?
 +
<br><br>A:&emsp; The general manufacturer does not want to do it at all, it will only increase the cost. The main reason is that the process of separating paper form plastics is complicated, all the entry would be supervised by government, and the obstacle is great. The only advantage is that you can get the money from the government (or from the paper manufacturer).
 +
<br><br>
 +
&emsp;&emsp;Views on disposable paper cups form Lian-Tai: I think the current propaganda is not to use disposable products, but there will be one-time products, but also because of his health problems, and now he has come back again, contradicting the original intention.
 +
Like a fast food restaurant, if it is not used after repeated use, it will cause health problems. In addition, if every fast-food restaurant has to add a sink or a regional cleaning thing, it will also increase the cost. If you give it to a big factory to wash it, it will definitely save more money than you need to clean it.
 +
<br><br>
 +
&emsp;&emsp;In fact, in terms of the types of recycling, while increasing the recycling category of paper containers, there have been many difficulties and the number of procedures has increased. Although the government's decision support has indeed increased the amount of recycling, it has not been reviewed from the entire process of recycling, but it has increased the complexity.
 +
<br>
 +
</p><br><br><br>
 +
<p class="second">Chung Hwa Pulp Corporation</p>
 +
                <div id="EX14" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/d/d7/T--CCU_Taiwan--JOE_experts_14.jpg" style="width:100%">
 +
                </div>
 +
 
 +
 
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper.
 +
</p>
 
<hr>
 
<hr>
−
<p class="description">Chung-Hsing University<br> <br>
+
<p class="first" id="ca5">Enterpreneumship</p>
−
Professor Yuan-Shing Perng: He helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system.<br><br>
+
<p class="second">Yong-Zhong Chen</p>
−
Professor Wen-Jau Lee: He told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties.<br>
+
<p class="description">CEO of THEFREEN BURGER
−
</p>
+
</p>
−
<hr>
+
 
−
<p class="description">Lian-Tai:<br> <br>
+
                <div id="EX15" class="polaroid">
−
Lian-Tai is the only manufacturer in Taiwan that can separate paper from plastic lining. He helped us understand Taiwan's current recycling environment and recycling issues, you can refer to more details in iHP.
+
                  <img src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--JOE_EXPEARTS_NEW05.jpg" style="width:100%">
−
</p>
+
                </div>
 +
<br><br>
 +
                <div id="EX16" class="polaroid">
 +
                  <img src="https://static.igem.org/mediawiki/2018/2/25/T--CCU_Taiwan--JOE_EXPEARTS_NEW06.jpg" style="width:100%">
 +
                </div>
 +
 
 +
<br><br>
 +
<p class="description">&emsp;&emsp;Yong-Zhong Chen made us think about how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society.
 +
</p>
 +
 +
 +
 +
 +
 +
<br><br><br>
 
       </div>
 
       </div>
 
</div>
 
</div>

Latest revision as of 08:52, 1 December 2018

ENGAGING EXPERTS


Gene Design

National Taiwan University

Professor K.S. Tung

Institute of Molecular and Cellular Biology
National Taiwan University



  Professor K.S. Tung provided us constitutive type of promoter (ADH1 promoter), and auxotrophic marker (LEU2, URA3, TRP1).




Professor C.T. Huang

Department of Biochemical Science and Technology
National Taiwan University


  Professor C.T. Huang suggested using constitutive promoter without inducing by methanol. That is GAP promoter which is commonly used in P. pastoris. He also recommended that we could choose commercial pGAPZ A vector from Invitrogen.

Material Properties

ITRI (Industrial Technology Research Institute)




  ITRI is a nonprofit R&D organization engaging in applied research and technical services. Founded in 1973, ITRI has played a vital role in transforming Taiwan's economy from a labor-intensive industry to a high-tech industry.




Dr. Cheng-Han Hsieh

(The fourth on the left, Dr. Hsieh)


Research Fellow - Lignin Analytics

  Dr. Cheng-Han Hsieh recommended that we have to control the LIGGREEN in micromolecular to make it have the melting point. He also gave us some directions for decomposing lignin-like material. The Bacillus subtilis may be our good choice.




Dr. Shu-Chuan Huang

(The fourth on the left, Dr. Huang)


Research Fellow – Material and Chemical Research

  Dr. Shu-Chuan Huang recommended that we should refer to the ISO 9000, ASTM D6400, and so on. Then, she recommended that we should cut the material into small pieces, carry out industrial composting, and detect the decomposition ratio.

National Chung Hsing University

Prof. Yuan-Shing Perng

Department of Forestry
National Chung Hsing University



  Prof. Yuan-Shing Perng helped us test whether the paper laminated with LIGGREEN can be separated into paper pulping and LIGGREEN. In the interview with professor Perng, he confirmed that a small amount of lignin mixed into the ordinary pulp will not affect the paper remaking process, maybe LIGGREEN can be separated, too. He taught us to operate the pulper, after 200 seconds of pulping and beating, LIGGREEN and the paper were indeed separated. This proved that LIGGREEN would not harm in the pulp recycling system.




Prof. Wen-Jau Lee

Department of Forestry
National Chung Hsing University



  Prof. Wen-Jau Lee told us that in the reaction forming LIGGREEN from monolignols bonding to oligomers, there are some bonding nodes would act. He suggested that the oligomers we produced were preferably two-dimensional chain systems, not three-dimensional, to have thermoplastic properties.

Recycling System

Recycling Fund Management Board

More Wu

Deputy executive secretary of Recycling Fund Management Board


  More Wu recommended that our products may need to consider the shelf life, because some paper containers such as milk carton are packaging beverage for a long time and the lining of them cannot be leach. And he mentioned that the government used the method of Administrative guidance and Subsidy incentive to try to improve the recovery yield.
However, some misunderstandings in the process led to false positives in the news, causing the public to throw paper containers into general garbage disposal.

Production Line

Bobsen Paper Corporation



  Bobsen Paper Corporation is responsible for producing coated paper and assisting downstream manufacturers in creating creases on paper. After our fundraising session, Bobsen agreed with us to arrange a corporate visit. Let us visit the factory and equipment of the paper laminating and answer our doubts about the production line.

Recycling Program

Prof. Ping-Mu Shu

Department of Earth and Environmental Science
National Chung Cheng University



  Prof. Ping-Mu Shu told us the truth of recycling in Taiwan, he also told us our product would not influence the paper recycled system because the amount of our LIGGREEN is very low and we do not need to set a recycled spot of LIGGREEN cups.

Manufacturers & Factories

Lian-Tai



  Liantai is the only manufacturer with the support from government and controlling the technology to recycle the paper containers and separated paper from the polyethylene lynin.

  The recycling volume of paper containers last year are 25,000 tons, which meant that only 15% of the paper containers had been recycled.

Q:  Professor Peng had said that the general paper industries could also separate paper from plastic. We wonder to know that why only your manufacture uses the separated technology but not afraid of others replace you?

A:  The general manufacturer does not want to do it at all, it will only increase the cost. The main reason is that the process of separating paper form plastics is complicated, all the entry would be supervised by government, and the obstacle is great. The only advantage is that you can get the money from the government (or from the paper manufacturer).

  Views on disposable paper cups form Lian-Tai: I think the current propaganda is not to use disposable products, but there will be one-time products, but also because of his health problems, and now he has come back again, contradicting the original intention. Like a fast food restaurant, if it is not used after repeated use, it will cause health problems. In addition, if every fast-food restaurant has to add a sink or a regional cleaning thing, it will also increase the cost. If you give it to a big factory to wash it, it will definitely save more money than you need to clean it.

  In fact, in terms of the types of recycling, while increasing the recycling category of paper containers, there have been many difficulties and the number of procedures has increased. Although the government's decision support has indeed increased the amount of recycling, it has not been reviewed from the entire process of recycling, but it has increased the complexity.




Chung Hwa Pulp Corporation



  Chung Hwa Pulp Corporation provided us simple paper pulping and making process, they also answered the raw materials of paper.

Enterpreneumship

Yong-Zhong Chen

CEO of THEFREEN BURGER





  Yong-Zhong Chen made us think about how to conduct market analysis to better develop LIGGREEN. He also reminded us to investigate the status of similar industries in the existing market, in order to better construct the business model and strengthen the link between LOGGREEN and society.