Difference between revisions of "Team:New York City"

 
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{{New_York_City}}
 
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<h1> Welcome to New York City 2018 iGEM Team! </h1>
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<p>A diverse team of mostly New York City area high school students! </p>
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<img src="https://i.imgur.com/otlUEQi.jpg" width = "600" height = "700">
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<h3>Project Description</h3>
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                <div class="navbar-brand"><a href="https://2018.igem.org/Team:New_York_City">New York City iGEM</a></div>
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<p> Huntington's Disease (HD) is an autosomal dominant disorder that leads to the progressive degeneration of neurons in the brain, which currently has no cure. HD is typically adult-onset and is characterized by a variety of symptoms including amnesia, involuntary movements, poor coordination, and impaired decision-making. Mutations in the huntingtin (HTT) gene causes HD, specifically a trinucleotide repeat of CAG that is abnormally repeated over 40 times. The goal of our project is to test the effectiveness of the plasmid that we generated last year, which targets and blocks endogenous faulty mRNA and releases a corrected RNA strand for proper protein synthesis of the HTT gene. The efficacy of this plasmid will be tested on Huntington cell lines. Since this plasmid has been designed with two different fluorophores to differentiate between the mutated and corrected strands, the effectiveness of this treatment will be tested by evaluating whether the intensity of the corrected fluorophore increases and the mutated fluorophore decreases in the Huntington cell lines.</p>
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                                role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
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                                Project
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                            </a>
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                            <div class="dropdown-menu" aria-labelledby="navbarPR">
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/HD">What is
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                                    Huntington's?</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Description">Description</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Design">Design</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Model">Model</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/InterLab">InterLab</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Results">Results</a>
 
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                        <li class="nav-item dropdown">
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                                Notebook
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                            </a>
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                            <div class="dropdown-menu" aria-labelledby="navbarNB">
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Notebook">Notebook</a>
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                            <a class="nav-link dropdown-toggle py-2 d-none d-md-inline-block" href="#" id="navbarHP"
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                                role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
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                                Human Practices
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                            </a>
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                            <div class="dropdown-menu" aria-labelledby="navbarHP">
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Human_Practices">Human
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                                    Practices</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Integrated_Practices">Integrated
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                                    Practices</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Public_Engagement">Public
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                                    Engagement</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Collaborations">Collaborations</a>
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                        <li class="nav-item dropdown">
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                                role="button" data-toggle="dropdown" aria-haspopup="true" aria-expanded="false">
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                                Team
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                            </a>
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                            <div class="dropdown-menu" aria-labelledby="navbarT">
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Team">Team</a>
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                                <a class="dropdown-item" href="https://2018.igem.org/Team:New_York_City/Attributions">Attributions</a>
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                            </div>
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                        </li>
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                    </ul>
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<div class="container text-center" align="center">
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<h3>Before you start</h3>
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    <div class="row">
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<p> Please read the following pages:</p>
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        <div class="col-sm-12" align="center">
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<ul>
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            <h1 class="display-4">Testing the efficacy of mRNA displacement technique in huntingtin cell lines to
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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                treat Huntington's Disease</h1>
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<li> <a href="https://2018.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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            <br/>
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<li> <a href="https://2018.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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        </div>
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</ul>
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    </div>
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    <div class="row">
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        <div class="col-sm-12" align="center">
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            <img class="headimage" src="https://static.igem.org/mediawiki/2018/1/1c/T--New_York_City--logo.PNG" width=100%/>
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        </div>
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<div class="clear extra_space"></div>
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    <video height="320" width="auto" controls>
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        <source src="https://static.igem.org/mediawiki/2018/9/9c/T--New_York_City--nyc_presentation.mp4">
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<div class="clear extra_space"></div>
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        </video>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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<div align="center">
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    <div class="container row teamRow text-center">
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                    <img class="card-img-top head" src="https://static.igem.org/mediawiki/2018/2/20/T--New_York_City--nyc_5.jpg">
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        </div>
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        <div class="col-sm-4">
  
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                    <img class="card-img-top head" src="https://static.igem.org/mediawiki/2018/2/20/T--New_York_City--nyc_1.jpg">
  
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        </div>
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<div class="clear extra_space"></div>
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                    <img class="card-img-top head" src="https://static.igem.org/mediawiki/2018/e/e0/T--New_York_City--nyc_2.jpg">
  
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        </div>
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    </div>
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    <div class="container row teamRow text-center">
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        <div class="col-sm-4">
  
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                    <img class="card-img-top head" src="https://static.igem.org/mediawiki/2018/4/4e/T--New_York_City--nyc_3.jpg">
  
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<div class="column third_size" >
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        </div>
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        <div class="col-sm-4">
  
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<h3> Uploading pictures and files </h3>
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                    <img class="card-img-top head" src="https://static.igem.org/mediawiki/2018/f/f2/T--New_York_City--bronze.jpg">
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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        </div>
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        <div class="col-sm-4">
  
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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                    <img class="card-img-top head" src="https://static.igem.org/mediawiki/2018/2/20/T--New_York_City--nyc_4.jpg">
  
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<div class="button_link">
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        </div>
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<a href="https://2018.igem.org/Special:Upload">
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UPLOAD FILES
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</a>
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<div class="container" align="center">
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        <div class="col-sm-4">
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            <div class="container">
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                <h2 class="display-4">WHAT</h2>
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                <p align="left" class="lead">Huntington's Disease (HD) is a genetic disorder that results in the breakdown of nerve cells in the
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                    brain. Although the disease affects the whole brain, the basal ganglia, which plays a critical role in
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                    motor functions and behavior, is most severely affected. Thus, symptoms of HD include involuntary
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                    movements, personality changes, and cognitive problems. Over time, the effects of HD is extremely
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                    debilitating, eventually rendering a patient unable to speak, walk, or even reason.</p>
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            <br>
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            <div class="container">
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                <h2 class="display-4">WHY</h2>
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                <p align="left" class="lead">HD is a result of a specific mutation in the huntingtin (HTT) gene, which results in a faulty production
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                    of the HTT protein, mHTT. The mutation is a trinucleotide repeat of the codon CAG, where 40 or more will
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                    lead to the expression of HD.</p>
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            </div>
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        </div>
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        <div class="col-sm-4" align="center">
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            <img src="https://static.igem.org/mediawiki/2018/d/d8/T--New_York_City--neuron.jpg" width=300>
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        <div class="col-sm-4">
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            <div class="container">
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                <h2 class="display-4">WHO</h2>
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                <p align="left" class="lead">The most common form of HD is adult-onset, affecting people between their 30s and 40s.
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                    Many people form families and have children by the time they are diagnosed with HD, which automatically
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                    gives their children a 50% chance of inheriting the disease. HD affects about 1 in every 10,000
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                    people worldwide.</p>
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            <br>
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            <div class="container">
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                <h2 class="display-4">HOW</h2>
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                <p align="left" class="lead">Our goal is to develop a cure to Huntington's Disease by employing RNA strand displacement
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                    technology to target erroneous mRNA within the affected cells and replace it with the corrected RNA
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                    strand for proper protein synthesis.
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                </p>
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2018.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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        <source src="https://static.igem.org/mediawiki/2018/0/06/T--New_York_City--Video.mp4" type="video/mp4">
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<div class="highlight decoration_B_full">
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="button_link">
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<a href="https://2018.igem.org/wiki/index.php?title=Team:New_York_City&action=edit">  
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EDIT PAGE
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</a>
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<div class="container" align="center">
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    <div class="row" align="center">
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        <div class="col-sm-4">
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            <a href="https://hdsa.org/"><img class="sponsor" src="https://static.igem.org/mediawiki/2018/a/a4/T--New_York_City--hdsa.png"/></a>
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        </div>
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        <div class="col-sm-4">
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            <a href="http://www.hdfoundation.org/"><img class="sponsor" src="https://static.igem.org/mediawiki/2018/8/86/T--New_York_City--hdf.png"/></a>
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        </div>
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        <div class="col-sm-4">
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            <a href="https://www.cerebralpalsy.org.au/"><img class="sponsor" src="https://static.igem.org/mediawiki/2018/0/03/T--New_York_City--cpa.png"/></a>
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        </div>
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            <a href="https://www.steptember.us/"><img class="sponsor" src="https://static.igem.org/mediawiki/2018/3/38/T--New_York_City--step.png"/></a>
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<div class="clear extra_space"></div>
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            <a href="https://nya.hdsa.org//"><img class="sponsor" src="https://static.igem.org/mediawiki/2018/6/61/T--New_York_City--ya.png"/></a>
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        <div class="col-sm-4">
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            <a href="https://hdsa.donordrive.com/index.cfm?fuseaction=donorDrive.participant&participantID=48528"><img class="sponsor" src="https://static.igem.org/mediawiki/2018/1/11/T--New_York_City--hope.png"/></a>
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<h3>Tips</h3>
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        </div>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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    <div class="container" align="center">
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<li>Be clear about what you are doing and how you plan to do this.</li>
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        <div class="row" align="center">
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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            <div class="col-sm-3">
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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            </div>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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            <div class="col-sm-2">
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2018.igem.org/Calendar">iGEM 2018 calendar</a> </li>
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                <a href="https://www.facebook.com/HDResolution/"><img class="social" src="https://static.igem.org/mediawiki/2018/6/61/T--New_York_City--fb.png"></a>
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<li>Have lots of fun! </li>
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</ul>  
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            <div class="col-sm-2">
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</div>
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                <a href="https://twitter.com/HDNYC_iGEM"><img class="social" src="https://static.igem.org/mediawiki/2018/4/46/T--New_York_City--twit.png"></a>
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                <a href="https://www.instagram.com/nyc_igem/"><img class="social" src="https://static.igem.org/mediawiki/2018/0/0a/T--New_York_City--ig.png"></a>
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<div class="highlight decoration_A_full">
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            </div>
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<h3>Inspiration</h3>
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            <div class="col-sm-3">
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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            </div>
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<ul>
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        </div>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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        <br />
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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    </div>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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</body>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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Latest revision as of 00:10, 5 December 2018

Testing the efficacy of mRNA displacement technique in huntingtin cell lines to treat Huntington's Disease






WHAT

Huntington's Disease (HD) is a genetic disorder that results in the breakdown of nerve cells in the brain. Although the disease affects the whole brain, the basal ganglia, which plays a critical role in motor functions and behavior, is most severely affected. Thus, symptoms of HD include involuntary movements, personality changes, and cognitive problems. Over time, the effects of HD is extremely debilitating, eventually rendering a patient unable to speak, walk, or even reason.


WHY

HD is a result of a specific mutation in the huntingtin (HTT) gene, which results in a faulty production of the HTT protein, mHTT. The mutation is a trinucleotide repeat of the codon CAG, where 40 or more will lead to the expression of HD.

WHO

The most common form of HD is adult-onset, affecting people between their 30s and 40s. Many people form families and have children by the time they are diagnosed with HD, which automatically gives their children a 50% chance of inheriting the disease. HD affects about 1 in every 10,000 people worldwide.


HOW

Our goal is to develop a cure to Huntington's Disease by employing RNA strand displacement technology to target erroneous mRNA within the affected cells and replace it with the corrected RNA strand for proper protein synthesis.