Difference between revisions of "Team:Paris Bettencourt"

 
(22 intermediate revisions by 3 users not shown)
Line 14: Line 14:
  
 
<div class='textbody'>
 
<div class='textbody'>
−
<p>Antibiotic overuse in livestock industry is one of the major drivers to the antibiotic resistance evolution; motivating calls to reduce, replace, and re-think the antibiotic usage in animals. Antimicrobial peptides (AMPs) are a promising alternative to conventional antibiotics. Recently, a class of chemically-synthesized, star-shaped AMPs has been shown to exhibit broad-spectrum antimicrobial activity while maintaining biocompatibility with mammalian cells. In this project, we combinatorially fused a set of known AMPs to structurally diverse, self-assembling protein cores to produce star-shaped complexes. Over 200 fusions were designed and expressed in a cell-free system, then screened for activity, biocompatibility, and membrane selectivity. In addition, we selected 4 AMPs for rational mutagenesis (~12000 variants), and a subset of fusions for molecular dynamic modeling, to identify features of surface charge and star geometry that impact AMP performance. Overall, we aim to create a novel class of selective, non-toxic AMPs which are biologically-produced.</p>
+
<p><b>StarCores</b> are these novel, biologically-synhesized, star-shaped Antimicrobial Peptide (AMP). Like antibiotics, AMPs can be used to kill harmful bacteria. Since AMPs attack bacteria differently than conventional antibiotics, <b>the resistance evolution occurs at a much slower rate.</b></br>
 +
 
 +
<p>We designed StarCores in response to the specific needs of veterinarians, farmers, and policymakers in France. Our goal was to provide a specific solution for killing Gram-negative bacteria on pig farms. Over the summer, we successfully designed, produced, tested, and optimized a novel class of bacteria-killing peptides with <b>high activity and reduced toxicity.</b></br>
 +
 
 +
<p>The story of our project unfolds sequentially. For the best experience of our wiki, we invite you to read the sections below in order, starting with <b>Integrated Human Practices (Human Practices I)</b>.</p>
 
</div>
 
</div>
  
Line 22: Line 26:
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Human_Practices'>
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Human_Practices'>
 
                 <div class='large-block-content'>
 
                 <div class='large-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/d/d6/T--Paris_Bettencourt--Public_Engagement.png">
+
                     <img src="https://static.igem.org/mediawiki/2018/5/54/T--Paris_Bettencourt--HP_Firstpage.jpg">
 
                 </div>
 
                 </div>
 
                 <div>Human Practices I </div>
 
                 <div>Human Practices I </div>
Line 37: Line 41:
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Design'>
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Design'>
 
                 <div class='medium-square-block-content'>
 
                 <div class='medium-square-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/4/4d/T--Paris_Bettencourt--Construct_design.png">
+
                     <img src="https://static.igem.org/mediawiki/2018/0/0d/T--Paris_Bettencourt--Design_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Design</div>
 
                 <div>Design</div>
Line 46: Line 50:
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Production'>
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Production'>
 
                 <div class='medium-square-block-content'>
 
                 <div class='medium-square-block-content'>
−
                      
+
                     <img src="https://static.igem.org/mediawiki/2018/7/71/T--Paris_Bettencourt--Production_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Production</div>
 
                 <div>Production</div>
Line 55: Line 59:
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Testing'>
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Testing'>
 
                 <div class='medium-square-block-content'>
 
                 <div class='medium-square-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/d/d2/T--Paris_Bettencourt--Killing_Assay.png ">
+
                     <img src="https://static.igem.org/mediawiki/2018/5/5e/T--Paris_Bettencourt--Testing_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Testing</div>
 
                 <div>Testing</div>
Line 64: Line 68:
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Modeling'>
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Modeling'>
 
                 <div class='medium-square-block-content'>
 
                 <div class='medium-square-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/6/65/T--Paris_Bettencourt--Modeling_AI.png">
+
                     <img src="https://static.igem.org/mediawiki/2018/3/3d/T--Paris_Bettencourt--Modeling_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Modeling</div>
 
                 <div>Modeling</div>
Line 73: Line 77:
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Optimization'>
 
               <a href='https://2018.igem.org/Team:Paris_Bettencourt/Optimization'>
 
                 <div class='medium-square-block-content'>
 
                 <div class='medium-square-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/d/d2/T--Paris_Bettencourt--Killing_Assay.png ">
+
                     <img src="https://static.igem.org/mediawiki/2018/d/df/T--Paris_Bettencourt--Optimization_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Optimization</div>
 
                 <div>Optimization</div>
Line 89: Line 93:
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Public_Engagement'>
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Public_Engagement'>
 
                 <div class='large-block-content'>
 
                 <div class='large-block-content'>
−
                   <img src="https://static.igem.org/mediawiki/2018/3/36/T--Paris_Bettencourt--VRPublic_Engagement.png">
+
                   <img src="https://static.igem.org/mediawiki/2018/7/71/T--Paris_Bettencourt--HPII_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Human Practices II (Public Engagement)</div>
 
                 <div>Human Practices II (Public Engagement)</div>
Line 101: Line 105:
 
     <div class='grid-container'>
 
     <div class='grid-container'>
 
         <div class='small-block'>
 
         <div class='small-block'>
−
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Team'>
+
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Members'>
 
                 <div class='small-block-content'>
 
                 <div class='small-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/3/3a/T--Paris_Bettencourt--Group_Work.png">
+
                     <img src="https://static.igem.org/mediawiki/2018/9/97/T--Paris_Bettencourt--Team_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Team</div>
 
                 <div>Team</div>
Line 130: Line 134:
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Medal_Criteria'>
 
             <a href='https://2018.igem.org/Team:Paris_Bettencourt/Medal_Criteria'>
 
                 <div class='small-block-content'>
 
                 <div class='small-block-content'>
−
                     <img src="https://static.igem.org/mediawiki/2018/f/f5/T--Paris_Bettencourt--Medal_Gold.png">
+
                     <img src="https://static.igem.org/mediawiki/2018/c/ca/T--Paris_Bettencourt--Medal_FirstPage.png">
 
                 </div>
 
                 </div>
 
                 <div>Medal Criteria</div>
 
                 <div>Medal Criteria</div>
Line 140: Line 144:
 
</div>
 
</div>
  
 +
<br>
 +
<br>
 +
<br>
 +
<div>
 +
<div class='textbody'>
 +
  <h3>Giant Jamboree 2018 Results!</h3>
 +
<br>
 +
  <img src="https://static.igem.org/mediawiki/2018/c/ca/T--Paris_Bettencourt--Medal_FirstPage.png" style="float:left;width:100px;height:100px;">
 +
  <b>Gold Medal </b><br>
 +
  Nominations for <b>Best New Composite Part </b> and <b>Best Software</b><br><b><a href="https://synbiobeta.com/cell-free-expression-platforms-enable-new-possibilities-at-igem-and-beyond/">Synbiobeta wrote an article on us! </b>
 +
</div>
 
</body>
 
</body>
  

Latest revision as of 00:50, 8 December 2018

Protein scaffolds for star-shaped antimicrobial peptides

StarCores are these novel, biologically-synhesized, star-shaped Antimicrobial Peptide (AMP). Like antibiotics, AMPs can be used to kill harmful bacteria. Since AMPs attack bacteria differently than conventional antibiotics, the resistance evolution occurs at a much slower rate.

We designed StarCores in response to the specific needs of veterinarians, farmers, and policymakers in France. Our goal was to provide a specific solution for killing Gram-negative bacteria on pig farms. Over the summer, we successfully designed, produced, tested, and optimized a novel class of bacteria-killing peptides with high activity and reduced toxicity.

The story of our project unfolds sequentially. For the best experience of our wiki, we invite you to read the sections below in order, starting with Integrated Human Practices (Human Practices I).

Human Practices I
Design
Production
Testing
Modeling
Optimization
Human Practices II (Public Engagement)
Team
Lab Notebook
BioBricks
Medal Criteria



Giant Jamboree 2018 Results!


Gold Medal
Nominations for Best New Composite Part and Best Software
Synbiobeta wrote an article on us!

Centre for Research and Interdisciplinarity (CRI)
Faculty of Medicine Cochin Port-Royal, South wing, 2nd floor
Paris Descartes University
24, rue du Faubourg Saint Jacques
75014 Paris, France
paris-bettencourt-2018@cri-paris.org