Difference between revisions of "Team:CUNY Kingsborough/Design"

 
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<h1>Design</h1>
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:CUNY_Kingsborough/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
 
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<h1 class="title-padding">Design</h1>
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<h3>What should this page contain?</h3>
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<li>Explanation of the engineering principles your team used in your design</li>
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<li>Discussion of the design iterations your team went through</li>
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<li>Experimental plan to test your designs</li>
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<h3>Inspiration</h3>
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
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<p class="low-rise-padding">The parts shown below apply to our study of the light operon.</p>
  
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<h2 class="low-rise-padding">Kill Switch for Engineered Bacteria</h2>
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<p class="low-rise-padding">In 2016, <a id="bodyLink" href="https://2016.igem.org/Team:Kingsborough_NY">we engineered E.coli to break down nitrogenous waste in Jamaica Bay</a>. In 2017, <a id="bodyLink" href="https://2017.igem.org/Team:Kingsborough_NY">we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant</a>. We had two iterations of this design - one producing MazF which causes high cell death and one producing LacZ which allowed us to collect data and analyze expression levels without having to worry about high toxicity. Both versions utilized the pDawn promoter (BBa K1616019) which we characterized this year using models.</p>
  
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<h3 class="low-rise-padding">Part# K2268006 (MazF)</h3>
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<center><img src="https://static.igem.org/mediawiki/2018/9/9f/T--CUNY_Kingsborough--pdawnmazfssra.jpeg" width="45%"></center>
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<h3 class="low-rise-padding">Part# K2268005 (LacZ)</h3>
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<center><img src="https://static.igem.org/mediawiki/2018/5/5b/T--CUNY_Kingsborough--pdawnlacz.jpeg" width=45%"></center>
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Latest revision as of 03:59, 8 December 2018

Background Design Experiments Results
Overview Modeling 101 Light Operon Turing Patterns CRISPR-Cas13a
Human Practices Public Engagement
Team Members Collaborations Attributions

Design

The parts shown below apply to our study of the light operon.

Kill Switch for Engineered Bacteria

In 2016, we engineered E.coli to break down nitrogenous waste in Jamaica Bay. In 2017, we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant. We had two iterations of this design - one producing MazF which causes high cell death and one producing LacZ which allowed us to collect data and analyze expression levels without having to worry about high toxicity. Both versions utilized the pDawn promoter (BBa K1616019) which we characterized this year using models.

Part# K2268006 (MazF)

Part# K2268005 (LacZ)