Difference between revisions of "Team:CUNY Kingsborough/Design"

 
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<h1 class="title-padding">Design</h1>
 
<h1 class="title-padding">Design</h1>
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<p class="low-rise-padding">The parts shown below apply to our study of the light operon.</p>
  
 
<h2 class="low-rise-padding">Kill Switch for Engineered Bacteria</h2>
 
<h2 class="low-rise-padding">Kill Switch for Engineered Bacteria</h2>
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<p class="no-rise-padding">In 2016, <a id="bodyLink" href="https://2016.igem.org/Team:Kingsborough_NY">we engineered E.coli to break down nitrogenous waste in Jamaica Bay</a>. In 2017, <a id="bodyLink" href="https://2017.igem.org/Team:Kingsborough_NY">we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant</a>. Within this design we had two versions - one with MazF which causes high cell death, and one with LacZ which allowed us to collect data on expression levels without having to worry about high toxicity.</p>
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<p class="low-rise-padding">In 2016, <a id="bodyLink" href="https://2016.igem.org/Team:Kingsborough_NY">we engineered E.coli to break down nitrogenous waste in Jamaica Bay</a>. In 2017, <a id="bodyLink" href="https://2017.igem.org/Team:Kingsborough_NY">we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant</a>. We had two iterations of this design - one producing MazF which causes high cell death and one producing LacZ which allowed us to collect data and analyze expression levels without having to worry about high toxicity. Both versions utilized the pDawn promoter (BBa K1616019) which we characterized this year using models.</p>
  
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<h2 class="low-rise-padding">Design of Light-Activated Kill Switch</h2>
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<h3 class="low-rise-padding">Part# K2268006 (MazF)</h3>
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<h3 class="low-rise-padding">Part# K2268005 (LacZ)</h3>
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<h3 class="low-rise-padding">K2268006</h3>
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<center><img src="https://static.igem.org/mediawiki/2018/5/5b/T--CUNY_Kingsborough--pdawnlacz.jpeg" width=45%"></center>
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Latest revision as of 03:59, 8 December 2018

Background Design Experiments Results
Overview Modeling 101 Light Operon Turing Patterns CRISPR-Cas13a
Human Practices Public Engagement
Team Members Collaborations Attributions

Design

The parts shown below apply to our study of the light operon.

Kill Switch for Engineered Bacteria

In 2016, we engineered E.coli to break down nitrogenous waste in Jamaica Bay. In 2017, we designed a light-activated kill switch to prevent our bacteria from surviving in saltwater outside of the waste treatment plant. We had two iterations of this design - one producing MazF which causes high cell death and one producing LacZ which allowed us to collect data and analyze expression levels without having to worry about high toxicity. Both versions utilized the pDawn promoter (BBa K1616019) which we characterized this year using models.

Part# K2268006 (MazF)

Part# K2268005 (LacZ)