Difference between revisions of "Team:CUNY Kingsborough/Collaborations"

 
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1 class="title-padding">Collaborations</h1>
  
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<h2 class="default-padding">Data Collection for the EtBr Spot Protocol</h2>
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<p class="low-rise-padding">We want our standard curve to be able to predict concentrations from varying levels of pixel intensity. In order to do so, we needed a larger and more diverse dataset. So we asked iGEM teams to perform the following task: dilute 1 uL of DNA of an unknown concentration into 9 uL EtBr (1 ng/uL), photograph the samples under UV light, and send us the images. Using ImageJ®, we compared the pixel intensity of the sample with a known DNA concentration to the predicted pixel intensity based on our standard curve. Special thanks to <b>HD Resolution</b> from the US and <b>Tec de Monterrey_Gdl</b> from Mexico! </p>
  
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<h1>Collaborations</h1>
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<h2 class="default-padding">Predicting Collateral Cleavage Activity of Cas13a</h2>
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Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.
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<h3>Silver Medal Criterion #2</h3>
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Complete this page if you intend to compete for the silver medal criterion #2 on collaboration. Please see the <a href="https://2018.igem.org/Judging/Medals">2018 Medals Page</a> for more information.
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<small>Cas9 - a cleaving RNA in CRISPR technology</small>
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<p class="low-rise-padding">We collaborated with the <b>Columbia University iGEM Team</b> to help them model the collateral cleavage activity of Cas13a, an enzyme used to cleave RNA in CRISPR technology. Our team provided the base system of equations and simulated the equations. <a id="bodyLink" href="https://2018.igem.org/Team:CUNY_Kingsborough/CRISPR-Cas13a">See the details here.</a></p>
  
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<h4> Which other teams can we work with? </h4>
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You can work with any other team in the competition, including software, hardware, high school and other tracks. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.
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<h2 class="default-padding">Exchanging Ideas</h2>
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In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.
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<p class="low-rise-padding"> Thanks to the <b>HD Resolution Team</b> in NYC, USA, we collaborated to host an event to present our projects to each other. They learned about the EtBr Spot Protocol and the light operon and we learned about their goal to cure Huntington's Disease. It was a great experience!</p>
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<small>Credits to Team HD Resolution</small>
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Here are some suggestions for projects you could work on with other teams:
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<li> Improve the function of another team's BioBrick Part or Device</li>
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<li> Characterize another team's part </li>
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<li> Debug a construct </li>
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<li> Model or simulate another team's system </li>
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<li> Test another team's software</li>
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<li> Help build and test another team's hardware project</li>
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<li> Mentor a high-school team</li>
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Latest revision as of 04:06, 8 December 2018

Background Design Experiments Results
Overview Modeling 101 Light Operon Turing Patterns CRISPR-Cas13a
Human Practices Public Engagement
Team Members Collaborations Attributions

Collaborations

Data Collection for the EtBr Spot Protocol

We want our standard curve to be able to predict concentrations from varying levels of pixel intensity. In order to do so, we needed a larger and more diverse dataset. So we asked iGEM teams to perform the following task: dilute 1 uL of DNA of an unknown concentration into 9 uL EtBr (1 ng/uL), photograph the samples under UV light, and send us the images. Using ImageJ®, we compared the pixel intensity of the sample with a known DNA concentration to the predicted pixel intensity based on our standard curve. Special thanks to HD Resolution from the US and Tec de Monterrey_Gdl from Mexico!

Predicting Collateral Cleavage Activity of Cas13a

Cas9 - a cleaving RNA in CRISPR technology

We collaborated with the Columbia University iGEM Team to help them model the collateral cleavage activity of Cas13a, an enzyme used to cleave RNA in CRISPR technology. Our team provided the base system of equations and simulated the equations. See the details here.

Exchanging Ideas

Thanks to the HD Resolution Team in NYC, USA, we collaborated to host an event to present our projects to each other. They learned about the EtBr Spot Protocol and the light operon and we learned about their goal to cure Huntington's Disease. It was a great experience!

Credits to Team HD Resolution