Difference between revisions of "Team:BioIQS-Barcelona"

 
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<h1> We are BioIQS iGEM team! </h1>
 
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<h3> Project description:</h3>
 
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<p>We would like to present you a brief description of our project on celiac disease:</p>
 
  
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<p>Our project is based on the design of a personalized gluten sensor by using the common tools of synthetic biology. There are already several sensors that are able to detect gluten in the food. However, there are milestones that still have not been overcome. We propose a robust model in which the HLA molecule of a patient is expressed and used as a sensor to detect specific reactive gluten epitopes. </p>
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<p>Coeliac disease (CD) is an autoimmune disorder that is closely related with HLA (Human leukocyte antigen), a type of receptor that are located in the surface of almost every cell of a human tissue, including white blood cells. These molecules are responsible for the correct discrimination between what is self and foreign proteins, guaranteeing the correct immune response against foreign agents that can cause infections. </p>
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<p>Within the HLA protein family there are the highly polymorphic HLA-DQ, which means that there are a lot of existing genetic variants of these molecules. Some of these HLA-DQ variants are able to recognize different gluten epitopes (aminoacid sequence of a protein) and present them to the T cells. More specifically, 25% of the general population carry the HLA-DQ that can recognize gluten derived epitopes, but only 1% of the population suffer from coeliac disease. The reason of such fact is that the inflammatory reaction is triggered by some T cells that can sense the gluten epitope presented by the HLA-DQ.</p>
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<p>Understanding why the inflammatory response is triggered by a certain type of T cells upon epitope presentation by HLA-DQ molecules is essential to uncover the mechanism of CD. Since the first step for the immune response activation is the recognition and binding of the gluten epitope to the HLA-DQ receptor, the development of a personalized sensor to determine reactive epitopes could help to better understand the disease and would also allow the screening of those foods that could potentially trigger an immune response to the patient.</p>
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<p>In our iGEM Project we will design a personalized gluten sensor through a synthetic biology approach. To do so, we decided to build a model based on the HLA expression of the patient which will be coupled to a sensor, allowing the detection of reactive epitopes. The main specificities of our sensor are described as follows:</p>
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<ul>
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<li>Since our sensor will be built according to the patient HLA, it will allow the detection of specific reactive epitopes independently of the food source.</li>
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<li>Additionally, our sensor will be able to detect reactive epitopes even in fermented foods.</li>
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<li>The methodology implemented in our sensor could be used for the identification of new reactive epitopes and unknown allelic variants.</li>
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<li>Our design requires only a DNA sample of the patient. Therefore, the methodology and application of our sensor could be extended for the detection of other HLA related disorders as well as the generation of new research lines for the diagnosis, detection and basic knowledge of these type of disorders.</li>
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<h1> Welcome to iGEM 2018! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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    <navigationbar></navigationbar>
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<li>  <a href="https://2018.igem.org/Competition">Competition Hub</a> </li>
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                        <h1 class="mb-5">We are the BioIQS iGEM team!</h1>
 +
                        <a href="https://2018.igem.org/Team:BioIQS-Barcelona#project" class="btn btn-outline btn-xl js-scroll-trigger">Explore our project!</a>
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                    <h2 class="section-heading orange">Welcome to our page</h2>
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                    <h3 class="orange">A brief resume:</h3>
  
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                    <div class="col-md-auto">
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                        <p class="book orange">Our project is based on the design of a <b>personalized gluten sensor</b> by using the common tools of <b>synthetic biology</b>. There are already several sensors that are able to detect gluten in the food. However, there are milestones that still have not been overcome. We propose a robust model in which the <b>HLA-DQ</b> protein of a patient is expressed and used as a sensor to detect specific reactive gluten epitopes.</p>
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                    </div>
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                    <a class="js-scroll-trigger a-arrow" href="https://2018.igem.org/Team:BioIQS-Barcelona#t-project"><span class="arrow down"></span></a>
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    </section>
  
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>  
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                <div class="col-md-5 image">
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                    <img src="https://static.igem.org/mediawiki/2018/2/26/T--BioIQS-Barcelona--2018_t-cell.png">
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                        <div class="col-md-11 col-lg-7">
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                            <p class="text-b"><b>Celiac disease (CD)</b> is an autoimmune disorder that is closely related with HLA (Human Leukocyte Antigen), a type of cell-surface proteins that are responsible for the regulation of the inmune systems in humans.</p>
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              <div class="col-mx-auto relative microscopi">
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                <div class="col-md-7 ">
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                    <p class="apart fletxa">These molecules are responsible for the correct discrimination between what is self and foreign proteins, guaranteeing the correct immune response against foreign agents that can cause infections.
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                    </p>
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                    <p class="text-b fletxa-d">
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                        Within the HLA protein family there is a subset called HLA-DQ. There are several DQ isoforms that can bind to different gluten-derived epitopes and present them to T-cells. More specifically, 25% of the general population carry the <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Celiac_disease">HLA-DQ haplotype</a> that recognizes gluten-derived epitopes, but only 1% of the population suffer from celiac disease.
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                    <p class="apart yellow">As a consequence of T-cell activation, an inmune response is triggered, causing damage to the enterocytes present in the small intestine.</p>
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                    <!--                    <img src="images/hla-solo.png">-->
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                            <p class="text-b">Understanding why the inflammatory response is triggered by a certain type of T cells upon epitope presentation by HLA-DQ molecules is essential to uncover the mechanism of CD. Since the first step for the immune response activation is the recognition and binding of the gluten epitope to the HLA-DQ receptor, we developed a sensor based on this principle.</p>
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                    </div>
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                </div>
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                <div class="col-md-8 end cnt block-desc-t">
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                    <p class="apart yellow">The development of a personalized sensor to detect reactive epitopes could help to better understand the disease and would also allow the screening of those foods that could potentially trigger an immune response to the patient.</p>
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                </div>
  
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                    <a href="https://2018.igem.org/Team:BioIQS-Barcelona#objectiv" class="text-transform js-scroll-trigger">Look our objectives!</a>
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            </div>
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    </section>
  
  
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                    <h2 class="section-heading orange">Objectives</h2>
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                    <h3 class="orange">Here there are:</h3>
  
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2018 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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                        <p class="book">In our iGEM Project we will design a
 +
                            <b>personalized gluten sensor</b> through a synthetic biology approach. To do so, we decided to build a <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Model">model</a> based on the HLA expression of the patient which will be coupled to a sensor, allowing the detection of reactive gluten epitopes.</p>
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                    </div>
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                    <a class="js-scroll-trigger a-arrow" href="https://2018.igem.org/Team:BioIQS-Barcelona#t-objectives"><span class="arrow down"></span></a>
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                </div>
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                            <p class="apart big">first</p>
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                            <p class="text-b">To obtain de HLA-DQ from scratch. That means, to <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Basic_Part" style="color: #032a5e; text-decoration: underline">extract</a> the &alpha; and &beta; chains <b>from the genomic DNA of a celiac patient.</b></p>
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                            <p class="apart big">second</p>
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                            <p class="text-b">To express the human HLA-DQ in a <b><a href="https://2018.igem.org/Team:BioIQS-Barcelona/Composite_Part" style="color: #032a5e; text-decoration: underline">bacterial</a> or yeast cell host.</b></p>
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                        <p class="apart big">third</p>
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                        <!--<img class="image" src="img/epitop.png" alt="Card image cap">-->
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                        <p class="text-b">To develop <b>non-dimensional mathematical and stochastic models</b> to understand and predict the <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Model"  style="color: #032a5e; text-decoration: underline">sensor dynamics.</a></p>
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                    <p class="apart big">fourth</p>
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                    <p class="text-b">To <a href="https://2018.igem.org/Team:BioIQS-Barcelona/Human_Practices" style="color: #032a5e; text-decoration: underline">provide information</a> to the public to <b>clear up common misunderstandings related to celiac disease.</b></p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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Latest revision as of 15:08, 10 December 2018

BIO IQS

We are the BioIQS iGEM team!

Explore our project!

Welcome to our page

A brief resume:

Our project is based on the design of a personalized gluten sensor by using the common tools of synthetic biology. There are already several sensors that are able to detect gluten in the food. However, there are milestones that still have not been overcome. We propose a robust model in which the HLA-DQ protein of a patient is expressed and used as a sensor to detect specific reactive gluten epitopes.

Celiac disease (CD) is an autoimmune disorder that is closely related with HLA (Human Leukocyte Antigen), a type of cell-surface proteins that are responsible for the regulation of the inmune systems in humans.

These molecules are responsible for the correct discrimination between what is self and foreign proteins, guaranteeing the correct immune response against foreign agents that can cause infections.

Within the HLA protein family there is a subset called HLA-DQ. There are several DQ isoforms that can bind to different gluten-derived epitopes and present them to T-cells. More specifically, 25% of the general population carry the HLA-DQ haplotype that recognizes gluten-derived epitopes, but only 1% of the population suffer from celiac disease.

As a consequence of T-cell activation, an inmune response is triggered, causing damage to the enterocytes present in the small intestine.

Understanding why the inflammatory response is triggered by a certain type of T cells upon epitope presentation by HLA-DQ molecules is essential to uncover the mechanism of CD. Since the first step for the immune response activation is the recognition and binding of the gluten epitope to the HLA-DQ receptor, we developed a sensor based on this principle.

The development of a personalized sensor to detect reactive epitopes could help to better understand the disease and would also allow the screening of those foods that could potentially trigger an immune response to the patient.

Look our objectives!

Objectives

Here there are:

In our iGEM Project we will design a personalized gluten sensor through a synthetic biology approach. To do so, we decided to build a model based on the HLA expression of the patient which will be coupled to a sensor, allowing the detection of reactive gluten epitopes.

first

To obtain de HLA-DQ from scratch. That means, to extract the α and β chains from the genomic DNA of a celiac patient.

second

To express the human HLA-DQ in a bacterial or yeast cell host.

third

To develop non-dimensional mathematical and stochastic models to understand and predict the sensor dynamics.

fourth

To provide information to the public to clear up common misunderstandings related to celiac disease.